Virulence characterization of Puccinia striiformis f. sp. tritici collections from six countries in 2013 to 2020

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat in Ethiopia. In total, 97 isolates were recovered from stripe rust samples collected in Ethiopia in 2013 and 2014. These isolates were tested on a set of 18 Yr single-gene differentials for characterization of races and 7 supplementary differentials for additional information of virulence. Of 18 P. striiformis f. sp. tritici races identified, the 5 most predominant races were PSTv-105 (21.7%), PSTv-106 (17.5%), PSTv-107 (11.3%), PSTv-76 (10.3%), and PSTv-41 (6.2%). High frequencies (>40%) were detected for virulence to resistance genes Yr1, Yr2, Yr6, Yr7, Yr8, Yr9, Yr17, Yr25, Yr27, Yr28, Yr31, Yr43, Yr44, YrExp2, and YrA. Low frequencies (<40%) were detected for virulence to Yr10, Yr24, Yr32, YrTr1, Hybrid 46, and Vilmorin 23. None of the isolates were virulent to Yr5, Yr15, YrSP, and YrTye. Among the six collection regions, Arsi Robe and Tiyo had the highest virulence diversities, followed by Bekoji, while Bale and Holeta had the lowest. Evaluation of 178 Ethiopian wheat cultivars and landraces with two of the Ethiopian races and three races from the United States indicated that the Ethiopian races were more virulent on the germplasm than the predominant races of the United States. Thirteen wheat cultivars or landraces that were resistant or moderately resistant to all five tested races should be useful for breeding wheat cultivars with resistance to stripe rust in both countries.

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Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici

Molecular Biology Reports ◽

10.1007/s11033-009-9823-9 ◽

2009 ◽

Vol 37 (2) ◽

pp. 1045-1052 ◽

Cited By ~ 45

Author(s):

Bo Liu ◽

Xiaodan Xue ◽

Suping Cui ◽

Xiaoyu Zhang ◽

Qingmei Han ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Rust Pathogen

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Virulence Characterization of Puccinia striiformis f. sp. tritici Using a New Set of Yr Single-Gene Line Differentials in the United States in 2010

Plant Disease ◽

10.1094/pdis-01-14-0071-re ◽

2014 ◽

Vol 98 (11) ◽

pp. 1534-1542 ◽

Cited By ~ 89

Author(s):

Anmin Wan ◽

Xianming Chen

Keyword(s):

United States ◽

Stripe Rust ◽

Large Scale ◽

Puccinia Striiformis ◽

Yellow Rust ◽

Single Gene ◽

The United States ◽

The Past ◽

History Of

Puccinia striiformis f. sp. tritici causes stripe rust (yellow rust) of wheat and is highly variable in virulence toward wheat with race-specific resistance. During 2010, wheat stripe rust was the most widespread in the recorded history of the United States, resulting in large-scale application of fungicides and substantial yield loss. A new differential set with 18 yellow rust (Yr) single-gene lines was established and used to differentiate races of P. striiformis f. sp. tritici, which were named as race PSTv in distinction from the PST races identified in the past. An octal system was used to describe the virulence and avirulence patterns of the PSTv races. From 348 viable P. striiformis f. sp. tritici isolates recovered from a total of 381 wheat and grass stripe rust samples collected in 24 states, 41 races, named PSTv-1 to PSTv-41, were identified using the new set of 18 Yr single-gene differentials, and their equivalent PST race names were determined on the previous set of 20 wheat cultivar differentials. The frequencies and distributions of the races and their virulences were determined. The five most predominant races were PSTv-37 (34.5%), PSTv-11 (17.5%), PSTv-14 (7.2%), PSTv-36 (5.2%), and PSTv-34 (4.9%). PSTv-37 was distributed throughout the country while PSTv-11 and PSTv-14 were almost restricted to states west of the Rocky Mountains. The races had virulence to 0 to 13 of the 18 Yr genes. Frequencies of virulences toward resistance genes Yr6, Yr7, Yr8, Yr9, Yr17, Yr27, Yr43, Yr44, YrTr1, and YrExp2 were high (67.0 to 93.7%); those to Yr1 (32.8%) and YrTye (31.3%) were moderate; and those to Yr10, Yr24, Yr32, and YrSP were low (3.4 to 5.7%). All of the isolates were avirulent to Yr5 and Yr15.

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Molecular Characterization of a Fus3/Kss1 Type MAPK from Puccinia striiformis f. sp. tritici, PsMAPK1

PLoS ONE ◽

10.1371/journal.pone.0021895 ◽

2011 ◽

Vol 6 (7) ◽

pp. e21895 ◽

Cited By ~ 24

Author(s):

Jun Guo ◽

Xiwei Dai ◽

Jin-Rong Xu ◽

Yulin Wang ◽

Pengfei Bai ◽

...

Keyword(s):

Molecular Characterization ◽

Puccinia Striiformis

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Cloning and characterization of a calcium binding EF-hand protein gene TaCab1 from wheat and its expression in response to Puccinia striiformis f. sp. tritici and abiotic stresses

Molecular Biology Reports ◽

10.1007/s11033-010-0501-8 ◽

2010 ◽

Vol 38 (6) ◽

pp. 3857-3866 ◽

Cited By ~ 26

Author(s):

Hao Feng ◽

Xiaomin Wang ◽

Yanfei Sun ◽

Xiaojie Wang ◽

Xianming Chen ◽

...

Keyword(s):

Calcium Binding ◽

Abiotic Stresses ◽

Protein Gene ◽

Puccinia Striiformis ◽

Ef Hand

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Development and characterization of novel microsatellite markers in Puccinia striiformis f.sp. tritici and their transferability in Puccinia species

Journal of Phytopathology ◽

10.1111/jph.12876 ◽

2019 ◽

Vol 168 (2) ◽

pp. 120-128

Author(s):

Siddanna Savadi ◽

Pramod Prasad ◽

Subhash Chander Bhardwaj ◽

Ruchi Rathore ◽

Kiran Sharma ◽

...

Keyword(s):

Microsatellite Markers ◽

Puccinia Striiformis

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Molecular Characterization of International Collections of the Wheat Stripe Rust Pathogen Puccinia striiformis f. sp. tritici Reveals High Diversity and Intercontinental Migration

Phytopathology ◽

10.1094/phyto-09-19-0355-r ◽

2020 ◽

Vol 110 (4) ◽

pp. 933-942 ◽

Cited By ~ 1

Author(s):

Dipak Sharma-Poudyal ◽

Qing Bai ◽

Anmin Wan ◽

Meinan Wang ◽

Deven See ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Yellow Rust ◽

Management Strategies ◽

Global Scale ◽

Asian Populations ◽

Wheat Diseases ◽

Geographic Regions ◽

High Level

Puccinia striiformis f. sp. tritici causes stripe rust (yellow rust), one of the most important wheat diseases worldwide. To understand the genetic variation of the pathogen in a global scale, 283 P. striiformis f. sp. tritici isolates collected from 16 countries in eight geographic regions were genotyped using 24 codominant simple sequence repeat markers. The overall collection had a high level of genetic diversity, and the diversity levels in the Asian populations were generally higher than those of the other regions. Heterozygosity of isolates ranged from 0 to 75%, with an average of 46%. Mean heterozygosity in individual countries ranged from 34 to 59%. A total of 265 multilocus genotypes (MLGs) were detected, which were classified into eight molecular groups. Some of the molecular groups were present in all geographic regions. Moreover, many isolates from different regions were found to be identical or very closely related MLGs. Analysis of molecular variance revealed high variation within countries and intermediate variation between countries, but it revealed low and insignificant variation among geographic regions. Pairwise comparisons of regional populations detected considerable effective migrants and only low to moderate levels of differentiation. The molecular genotypes had a moderate level of correlation with the virulence phenotypes, and some of the molecular/virulence groups contained isolates from different continents. The results indicate tremendous migrations of P. striiformis f. sp. tritici and warrant the development of management strategies considering the global pathogen population.

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Development and characterization of novel wheat-rye 1RS•1BL translocation lines with high resistance to Puccinia striiformis f. sp. tritici

Phytopathology ◽

10.1094/phyto-07-21-0313-r ◽

2022 ◽

Author(s):

Tianheng Ren ◽

Qing Jiang ◽

Zixin Sun ◽

Zhenglong Ren ◽

Feiquan Tan ◽

...

Keyword(s):

Stripe Rust ◽

High Resistance ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Sources Of Resistance ◽

Chinese Landrace ◽

New Gene ◽

Improvement Programs ◽

Translocation Lines

Wheat-rye 1RS•1BL translocations from Petkus rye have contributed substantially to wheat production worldwide with their great disease resistance and yield traits. However, the resistance genes on the 1RS chromosomes have completely lost their resistance to newly emerged pathogens. Rye could widen the variation of 1RS as a naturally cross-pollinated related species of wheat. In this study, we developed three new 1RS•1BL translocation lines by crossing rye inbred line BL1, selected from Chinese landrace rye Baili, with wheat cultivar Mianyang11. These three new translocation lines exhibited high resistance to the most virulent and frequently occurring stripe rust pathotypes and showed high resistance in the field where stripe rust outbreaks have been most severe in China. One new gene for stripe rust resistance, located on 1RS of the new translocation lines, is tentatively named YrRt1054. YrRt1054 confers resistance to Puccinia striiformis f. sp. tritici pathotypes that are virulent toward Yr9 and YrCn17. This new resistance gene, YrRt1054, is available for wheat improvement programs. The present study indicated that rye cultivars may carry additional untapped variation as potential sources of resistance.

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