Filter systems have the ability to remove pollutants from wastewater. However, to increase the treatment efficiency, the filter made of plants has been recommended which has been called the biofilter. The objective of this study was to examine the performance of biofilter made by Arundo donax L. on domestic wastewater treatment. In this paper, a laboratory study using vertical flow constructed wetlands planted with Arundo donax L. and without any other plants were conducted. For the biofilter planted in Arundo donax L., the removal of TN, NH4+-N and TP 89%, 93% and 98% respectively. In particular, we also got the nitrogen and phosphorus content of different parts of Arundo donax L. which was divided into root, stem and leaves. For the total nitrogen content in the plant, there is a quantitive relationship of stem<root<leaves. For the total phosphorus content, the relationship is root<stem<leaves quantitatively. Whilst, compared with the total removal of nitrogen and phosphorus, the removal via plant absorption accounts for 22% and 29% separately. The results of this research suggest that Arundo donax L. with large leaves could be used as a main plant in the domestic wastewater biofilter.