Cholinesterase activity as a potential biomarker for neurotoxicity induced by pesticides in vivo exposed Oreochromis niloticus (Nile tilapia)

2021 ◽  
pp. 1-25
Author(s):  
Muhammad Amin ◽  
Masarrat Yousuf ◽  
Mohammad Attaullah ◽  
Naveed Ahmad ◽  
Mohamad Nor Azra ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Cholinesterase Activity ◽  
Potential Biomarker

scholarly journals Efficacy and safety of boric acid as a preventive treatment against Saprolegnia infection in Nile tilapia (Oreochromis niloticus)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shimaa E. Ali ◽  
Amr A. A. Gamil ◽  
Ida Skaar ◽  
Øystein Evensen ◽  
Harrison Charo-Karisa
Keyword(s):  
Boric Acid ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Negative Impact ◽  
Preventive Treatment ◽  
Economic Losses ◽  
Continuous Exposure ◽  
Prophylactic Measure

AbstractSaprolegniosis is a worldwide fungal-like infection affecting freshwater fishes and their eggs. Reports show high mortalities and subsequent economic losses annually from Saprolegnia infections. Most therapeutants against Saprolegnia spp. infections are inefficient and some have negative impact on the environment. In this study, we have investigated the ability of boric acid (BA) to prevent Saprolegnia infection in Nile tilapia (Oreochromis niloticus). BA inhibited radial growth of Saprolegnia hyphae in vitro. Complete in vitro growth inhibition was found at a concentration of ≥0.6 g/L. Inhibitory effects were also observed in vivo when Nile tilapia were experimentally challenged with Saprolegnia spores and followed over 10 days post challenge and under continuous exposure to different BA concentrations. No signs of saprolegniosis were observed in fish treated with BA at concentrations of 0.4 g/L and above. Comet assay revealed that BA has low toxicity in tilapia continuously exposed to concentrations of 0.2–0.6 g/L for 96 h. Additionally, no significant histomorphological changes were observed in BA-treated fish compared to non-treated controls. Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) enzyme levels indicated reduction in systemic tissue damage associated with Saprolegnia infection. This study demonstrates the potential of BA as a prophylactic measure against Saprolegnia infection in tilapia, and we recommend additional studies on environmental impact.

The in vivo roles of galectin-2 from Nile tilapia (Oreochromis niloticus) in immune response against bacterial infection

2020 ◽  
Vol 106 ◽  
pp. 473-479
Author(s):  
Jinzhong Niu ◽  
Xinchao Liu ◽  
Zhiqiang Zhang ◽  
Yu Huang ◽  
Jufen Tang ◽  
...  
Keyword(s):  
Immune Response ◽  
Bacterial Infection ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Shrimp lectin (Md-Lec) conjugated copper sulfide nanoparticles enhance the elimination of aquatic pathogens in infected Nile tilapia (Oreochromis niloticus)

RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 44216-44224
Author(s):  
Abdul Salam Rubeena ◽  
Sreeja Lakshmi ◽  
Digi George ◽  
Siva Bala Subramaniyan ◽  
Anbazhagan Veerappan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Oreochromis Niloticus ◽  
Copper Sulfide ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Aquatic Pathogens

Synthesis of Md-Lec-pCuSNPs and its enhanced in vitro and in vivo antibacterial activity.

scholarly journals The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.)

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 34
Author(s):  
Verena Mertes ◽  
Alexander Kashulin Bekkelund ◽  
Leidy Lagos ◽  
Elia Ciani ◽  
Duncan Colquhoun ◽  
...  
Keyword(s):  
Fish Species ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Gadus Morhua ◽  
Atlantic Cod ◽  
Membrane Vesicles ◽  
In Vivo Studies ◽  
Vaccine Candidates ◽  
Nile Tilapia Oreochromis Niloticus

Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens Francisella noatunensis ssp. noatunensis and Francisella orientalis. The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we tested isolated membrane vesicles (MVs) as possible vaccine candidates based on previous trials in zebrafish (Danio rerio) indicating promising vaccine efficacy. Here, the MV vaccine-candidates were tested in their natural hosts, Atlantic cod (Gadus morhua L.) and Nile tilapia (Oreochromis niloticus). Injection of MVs did not display any toxicity or other negative influence on the fish and gene expression analysis indicated an influence on the host immune response. However, unlike in other tested fish species, a protective immunity following vaccine application and immunization period could not be detected in the Atlantic cod or tilapia. Further in vivo studies are required to achieve a better understanding of the development of immunological memory in different fish species.

scholarly journals Transcriptional knockout of steroidogenic factor 1 in vivo in Oreochromis niloticus increased weight and suppressed gonad development using antisense RNA

2021 ◽  
Author(s):  
Zhe Cao ◽  
Jun Qiang ◽  
Jun Zhu ◽  
Hong Li ◽  
Yi Tao ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Antisense Rna ◽  
Nile Tilapia ◽  
Gonad Development ◽  
External Genitalia ◽  
Cloning And Expression ◽  
Steroidogenic Factor 1 ◽  
And Function

Abstract Steroidogenic factor 1 (sf1) is an important regulator of gonad development and function in mammals. However, study of sf1 in fish is limited to cloning and expression and in vitro experiments. Using antisense RNA we knockout transcription of the sf1 gene in Nile tilapia Oreochromis niloticus, and obtain experimental fish in vivo. We demonstrate that antisense RNA can silence sf1 transcription and protein expression, and report suppression of sf1 transcription to affect gonad development and external genitalia formation in Nile tilapia. We also report disfunction of retinal metabolism and fatty acid metabolism to be important causes of weight gain and gonad abnormality with sf1 suppression. The feasibility of using antisense RNA for gene editing in fish is verified, and a new way of studying gene function and performing biological breeding is presented.

scholarly journals LECT2 Protects Nile Tilapia (Oreochromis niloticus) Against Streptococcus agalatiae Infection

2021 ◽  
Vol 12 ◽  
Author(s):  
Qi Li ◽  
Zhiqiang Zhang ◽  
Weiqi Fan ◽  
Yongxiong Huang ◽  
Jinzhong Niu ◽  
...  
Keyword(s):  
Immune Response ◽  
Bacterial Infection ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Head Kidney ◽  
Transcriptional Level ◽  
Reading Frame ◽  
Tissue Damages

Leukocyte cell-derived chemotaxin 2 (LECT2) is a multifunctional cytokine that especially plays an important role in innate immune. However, the roles of LECT2 in the immune response of the economically important fish Nile tilapia (Oreochromis niloticus) against bacterial infection remains unclear. In this study, a lect2 gene from Nile tilapia (On-lect2) was identified, and its roles in the fish’s immune response against bacterial infection were determined and characterised. On-lect2 contains an open reading frame of 456 bp that encodes a peptide of 151 amino acids, as well as the conservative peptidase M23 domain. On-LECT2 is 62%–84% identical to other fish species and about 50% identical to mammals. The highest transcriptional level of On-lect2 was detected in the liver, whereas the lowest levels were detected in the other tissues. Moreover, the On-LECT2 protein is located mainly in the brain and head kidney. The transcriptional levels of On-lect2 substantially increased in the head kidney, brain, liver and spleen after Streptococcus agalactiae infection. Knockdown On-lect2 led to higher mortality due to liver necrosis or haemorrhage and splenomegaly. In vitro analysis indicated that the recombinant protein of On-LECT2 improved phagocytic activity of head kidney-derived macrophages. In vivo challenge experiments revealed several functions of On-LECT2 in the immune response of Nile tilapia against bacterial infection, including promotion of inflammation, reduction of tissue damages and improvement of survival rate.

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