scholarly journals The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.)

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 34
Author(s):  
Verena Mertes ◽  
Alexander Kashulin Bekkelund ◽  
Leidy Lagos ◽  
Elia Ciani ◽  
Duncan Colquhoun ◽  
...  

Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens Francisella noatunensis ssp. noatunensis and Francisella orientalis. The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we tested isolated membrane vesicles (MVs) as possible vaccine candidates based on previous trials in zebrafish (Danio rerio) indicating promising vaccine efficacy. Here, the MV vaccine-candidates were tested in their natural hosts, Atlantic cod (Gadus morhua L.) and Nile tilapia (Oreochromis niloticus). Injection of MVs did not display any toxicity or other negative influence on the fish and gene expression analysis indicated an influence on the host immune response. However, unlike in other tested fish species, a protective immunity following vaccine application and immunization period could not be detected in the Atlantic cod or tilapia. Further in vivo studies are required to achieve a better understanding of the development of immunological memory in different fish species.

2018 ◽  
Vol 23 (3) ◽  
pp. 193
Author(s):  
Samuel Samuel ◽  
Yoga Candra Ditya ◽  
Vipen Adiansyah

Introduksi ikan nila atau “serapia” (Oreochromis niloticus) di Danau Paniai bertujuan untuk meningkatkan diversitas hasil tangkapan dan mengurangi tekanan eksploitasi terhadap jenis ikan endemik. Pertumbuhan dan produksi ikan nila yang cepat tanpa diiringi upaya pengelolaan akan mengancam keberlanjutan ikan endemik di danau. Upaya pengelolaan sumberdaya ikan di suatu perairan membutuhkan informasi dinamika populasi. Penelitian dinamika dan pengelolaan populasi ikan nila dilakukan pada bulan Februari sampai Oktober 2016. Contoh ikan dikumpulkan dari hasil tangkapan nelayan dengan menggunakan jaring insang dengan berbagai ukuran mata jaring dan dari 4 (empat) enumerator pada tujuh stasiun pengamatan. Hasil penelitian menunjukkan populasi ikan nila di Danau Paniai didominasi ukuran panjang individu antara 15-25 cm sebanyak 67,24%. Pola pertumbuhan ikan jantan dan betina adalah isometrik, panjang maksimum rata-rata (L) adalah 37,28 cm dan koefisien pertumbuhan (K) adalah 0,50 per tahun. Mortalitas alami (M) dan mortalitas penangkapan (F) masing-masing sebesar 0,99 dan 0,54 per tahun. Tingkat eksploitasi (E) diperoleh nilai 0,35 lebih kecil dari nilai optimum (E=0,5). Ukuran rata-rata ikan nila tertangkap (Lc) adalah 20,55 cm lebih besar dari ukuran pertama matang gonad (Lm) sebesar 14,73 cm. Nilai Lc>Lm mengindikasikan sebagian besar populasi ikan nila di Danau Paniai sempat melakukan pemijahan sehingga pemanfaatan lebih atau sama dengan nilai optimum diharapkan dapat meningkatkan pemanfataan ikan nila di Danau Paniai. Introduction of nile tilapia (Oreochromis niloticus) in Lake Paniai known as “serapia” is aimed for improving the diversity of catches and reducing the exploitation pressure on the endemic fish species. The rapid growth, reproduction and production of nile tilapia without its management efforts is a new threat to the sustainability of the existence and utilization of endemic fish species in the lake. Management efforts of fish resources require population dynamics information. Research on the dynamics and management of nile tilapia populations was conducted from February to October 2016. Fish samples were collected from fishermen catches using nets with various mesh sizes and from four enumerators at seven observation stations. The results showed that the population of nile tilapia in Lake Paniai was dominated by individual length between 15-25 cm with frequency of 67,24%. The growth pattern of male and female fish were isometric, the average maximum length (L) was 37.28 cm and the growth coefficient (K) was 0.50 per year. Natural mortality (M) and fishing mortality (F) were 0.99 and 0.54 per year respectively. Exploitation rate (E) of 0.35 was smaller than the optimum value (E=0.5). The average size of nile tilapia captured (Lc) was 20.55 cm larger than the first size of gonad maturity (Lm) of 14.73 cm. The Lc value was higher than that the Lm value(Lc>Lm) indicating that most of nile tilapia population in Lake Paniai has spawned so that increasing the more or equal to the optimum value was expected to improve the of nile tilapia fish in Lake Paniai.


2020 ◽  
Vol 41 (1) ◽  
pp. 351
Author(s):  
Gláucia Frasnelli Mian ◽  
Charles Oswaldo Roncancio ◽  
Maria Cristina de Souza Silva ◽  
Amanda Carvalho Rosado Ferreira ◽  
Dircéia Aparecida da Costa Custódio ◽  
...  

Nile tilapia (Oreochromis niloticus) has always been considered more resistant to diseases compared to other fish species. However, in recent decades, this fish species has also been susceptible to many bacterial, fungal, parasitic, and nutritional diseases. In Brazil, streptococcosis is one of the main diseases that affect tilapia and has caused many economic losses. A resistance test was conducted with 93 tilapia of four varieties (UFLA strain and three commercial varieties: SC1, SC2, and SC3) to evaluate resistance to experimental inoculation with Streptococcus agalactiae. Each variety was distributed in five aquariums, with nine specimens of the same variety. Among the five aquariums, four housed fish were intraperitoneally inoculated with 107 CFU/fish of S. agalactiae and one aquarium harbored fish from the control group, which received 0.1 mL of sterile BHI (Brain Heart Infusion). Significant differences were observed in the survival rates accumulated at the end of the experiment (day 15), with values of 22, 36, 36, and 15% for SC1, SC2, SC3, and UFLA, respectively. The UFLA strain presented lower survival than other strains, which did not differ among the 15 days of challenge. These results demonstrate that there are animals with a better response to the resistance test than others.


2020 ◽  
Vol 106 ◽  
pp. 473-479
Author(s):  
Jinzhong Niu ◽  
Xinchao Liu ◽  
Zhiqiang Zhang ◽  
Yu Huang ◽  
Jufen Tang ◽  
...  

RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 44216-44224
Author(s):  
Abdul Salam Rubeena ◽  
Sreeja Lakshmi ◽  
Digi George ◽  
Siva Bala Subramaniyan ◽  
Anbazhagan Veerappan ◽  
...  

Synthesis of Md-Lec-pCuSNPs and its enhanced in vitro and in vivo antibacterial activity.


Mutagenesis ◽  
2014 ◽  
Vol 29 (3) ◽  
pp. 215-219 ◽  
Author(s):  
Karina M. Melo ◽  
Cesar K. Grisolia ◽  
Julio C. Pieczarka ◽  
Ludmilla R. de Souza ◽  
José de Souza Filho ◽  
...  

DEPIK ◽  
2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Auliyanisa Khayra ◽  
Zainal A. Muchlisin ◽  
Muhammad A. Sarong

Abstract. The objective of the present study was to analyze the morphometric variationsof five predominant fish species caught in Lake Aneuk Laot, Sabang City,Weh Island.The study was conducted in December 2015 to January 2016. The survey method was used in this study. The fish target were Nile tilapia (Oreochromis niloticus), tilapia (O. mossambicus), snakeskin gourami (Trichopodus pectoralis), giant gourami (Osphronemus goramy) and snakehead (Channa striata). The fish samples were catched using gillnets. The fish sample were analyzed for traditional and truss network morphometrics. The results showed that there is closed morphological relationship between Nile tilapia and tilapia, and between snakeskin gourami and giant gourami, while the snakehead fish was discriminated apart from the two previous groups.Keywords: Morphometric Nile tilapia, tilapia, snakeskin gourami, giant gourami, snakehead fish, Weh Island Abstrak. Penelitian ini bertujuan untuk menganalisis variasi morfologi ikan hasil tangkapan dominan di Danau Aneuk Laot Kota Sabang dengan menggunakan teknik traditional dan truss morphometric. Penelitian dilakukan pada Desember 2015 sampai Januari 2016. Ikan sampel terdiri dari ikan nila (Oreochromis niloticus), ikan mujair (O. mossambicus), ikan sepat siam (Trichopodus pectoralis), ikan gurami (Osphronemus gorami) dan ikan gabus (Channa striata). Ikan sampel diperoleh dengan menggunakan alat tangkap jaring. Sebanyak 12 karakter traditional morphometric and 14 karakter truss dianalisis dalam penelitian ini.  Hasil penelitian menunjukkan terdapat tiga kelompok ikan, kelompok pertama ikan nila dan ikan mujair, kedua ikan ini kemiripan yang tinggi; kelompok kedua adalah ikan sepat siam dan ikan gurami, kelompok ketiga adalah ikan gabus yang memiliki kemiripan yang jauh dengan kedua kelompok ikan sebelumnya.Kata kunci: Morfometrik, Nila, mujair, sepat siam, gurami, gabus, Pulau Weh


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