Biodiesel synthesis in a solvent-free system by recombinant Rhizopus oryzae: comparative study between a stirred tank and a packed-bed batch reactor

2017 ◽  
Vol 35 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Albert Canet ◽  
KÍrian Bonet-Ragel ◽  
M. Dolors Benaiges ◽  
Francisco Valero
Keyword(s):  
Comparative Study ◽  
Rhizopus Oryzae ◽  
Batch Reactor ◽  
Packed Bed ◽  
Solvent Free ◽  
Stirred Tank ◽  
Free System ◽  
Biodiesel Synthesis ◽  
Solvent Free System

scholarly journals Performance of an enzymatic packed bed reactor running on babassu oil to yield fatty ethyl esters (FAEE) in a solvent-free system

2015 ◽  
Vol 2 (2) ◽  
pp. 242-247 ◽  
Author(s):  
Aline Simões ◽  
Lucas Ramos ◽  
Larissa Freitas ◽  
Julio C. Santos ◽  
Gisella M. Zanin ◽  
...  
Keyword(s):  
Packed Bed ◽  
Solvent Free ◽  
Ethyl Esters ◽  
Packed Bed Reactor ◽  
Free System ◽  
Babassu Oil ◽  
Solvent Free System

Immobilized recombinant Rhizopus oryzae lipase for the production of biodiesel in solvent free system

2010 ◽  
Vol 67 (1-2) ◽  
pp. 45-51 ◽  
Author(s):  
You-dong Wang ◽  
Xiao-yong Shen ◽  
Zhi-lin Li ◽  
Xun Li ◽  
Fei Wang ◽  
...  
Keyword(s):  
Rhizopus Oryzae ◽  
Solvent Free ◽  
Rhizopus Oryzae Lipase ◽  
Free System ◽  
Solvent Free System

scholarly journals Continuous Production of 2-Phenylethyl Acetate in a Solvent-Free System Using a Packed-Bed Reactor with Novozym® 435

Catalysts ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 714 ◽  
Author(s):  
Shang-Ming Huang ◽  
Hsin-Yi Huang ◽  
Yu-Min Chen ◽  
Chia-Hung Kuo ◽  
Chwen-Jen Shieh
Keyword(s):  
Chemical Synthesis ◽  
Flow Rate ◽  
Packed Bed ◽  
Solvent Free ◽  
Packed Bed Reactor ◽  
Packed Bed Bioreactor ◽  
Free System ◽  
Phenethyl Alcohol ◽  
Solvent Free System ◽  
Molar Conversion

2-Phenylethyl acetate (2-PEAc), a highly valued natural volatile ester, with a rose-like odor, is widely added in cosmetics, soaps, foods, and drinks to strengthen scent or flavour. Nowadays, 2-PEAc are commonly produced by chemical synthesis or extraction. Alternatively, biocatalysis is a potential method to replace chemical synthesis or extraction for the production of natural flavour. Continuous synthesis of 2-PEAc in a solvent-free system using a packed bed bioreactor through immobilized lipase-catalyzed transesterification of ethyl acetate (EA) with 2-phenethyl alcohol was studied. A Box–Behnken experimental design with three-level-three-factor, including 2-phenethyl alcohol (2-PE) concentration (100–500 mM), flow rate (1–5 mL min−1) and reaction temperature (45–65 °C), was selected to investigate their influence on the molar conversion of 2-PEAc. Then, response surface methodology and ridge max analysis were used to discuss in detail the optimal reaction conditions for the synthesis of 2-PEAc. The results indicated both 2-PE concentration and flow rate are significant factors in the molar conversion of 2-PEAc. Based on the ridge max analysis, the maximum molar conversion was 99.01 ± 0.09% under optimal conditions at a 2-PE concentration of 62.07 mM, a flow rate of 2.75 mL min−1, and a temperature of 54.03 °C, respectively. The continuous packed bed bioreactor showed good stability for 2-PEAc production, enabling operation for at least 72 h without a significant decrease of conversion.

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