Hesperozygis ringens (Benth.) Epling essential oil: antifungal activity and effect on ergosterol content of wood-decay fungi

Abstract The chemical composition of the essential oil of Chamaecyparis formosensis wood has been examined. GC-MS data and retention indices for reference samples were used to identify 32 constituents. α-Eudesmol (18.06%), β-guaiene (8.0%), (–)-β-cadinene (7.89%), γ-costal (7.03%), α-muurolol (6.49%), 4α-hydroxy-4β-methyldihydrocostol (5.52%), σ-selinene (4.78%), santolina triene (4.60%), eremophilene (4.32%), humulene (4.11%), myrtenol (4.11%), and τ-cadinene (3.25%) were the most abundant components. Tests with the typical wood decay fungi, Laetiporus sulphureus and Trametes versicolor, proved the antifungal activity of the oil, as the growth of L. sulphureus and T. versicolor was inhibited at concentrations of 50 and 100 μg ml−1, respectively. The following characteristic volatile compounds were isolated and purified from ethyl acetate fractions: epi-cubenol, chamaecynone, myrtenol, cis-myrtanol, 12-hydroxyisointermedenol and 4α-hydroxy-4β-methyldi-hydrocostol. Chamaecynone possessed the strongest antifungal activity, with an antifungal index of 88.2% and 67.3% for L. sulphureus and T. versicolor at a dose of 50 μg ml−1, respectively.

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The Composition, Anti-mildew and Anti-wood-decay Fungal Activities of the Leaf and Fruit Oils of Juniperus Formosana from Taiwan

Natural Product Communications ◽

10.1177/1934578x1300800936 ◽

2013 ◽

Vol 8 (9) ◽

pp. 1934578X1300800 ◽

Cited By ~ 4

Author(s):

Yu-Chang Su ◽

Kuan-Ping Hsu ◽

Eugene I-Chen Wang ◽

Chen-Lung Ho

Keyword(s):

Essential Oil ◽

Wood Decay ◽

Decay Fungi ◽

Active Source ◽

Wood Decay Fungi ◽

Leaf Oil ◽

Fruit Oil ◽

Type Apparatus

In this study, anti-mildew and anti-wood-decay fungal activities of the leaf and fruits essential oil and its constituents from Juniperus formosana were evaluated in vitro against seven mildew fungi and four wood decay fungi, respectively. The main compounds responsible for the anti-mildew and anti-wood-decay fungal activities were also identified. The essential oil from the fresh leaves and fruits of J. formosana were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The leaf oil mainly consisted of α-pinene (41.0%), limonene (11.5%), α-cadinol (11.0%), elemol (6.3%), and β-myrcene (5.8%); the fruit oil was mostly α-pinene (40.9%), β-myrcene (32.4%), α-thujene (5.9%) and limonene (5.9%). Comparing the anti-mildew and anti-wood-decay fungal activities of the oils suggested that the leaf oil was the most effective. For the anti-mildew and anti-wood-decay fungal activities of the leaf oil, the active source compounds were determined to be α-cadinol and elemol.

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DAYA HAMBAT EKSTRAK KULIT JATI (Tectona grandis Linn F) TERHADAP PERTUMBUHAN JAMUR PELAPUK KAYU Schizophyllum commune Fries

JURNAL HUTAN LESTARI ◽

10.26418/jhl.v7i3.36433 ◽

2019 ◽

Vol 7 (3) ◽

Author(s):

Ferry Vernando Hutabarat ◽

Farah Diba ◽

Lolyta Sisillia

Keyword(s):

Antifungal Activity ◽

Screening Test ◽

Schizophyllum Commune ◽

Wood Decay ◽

Petri Dish ◽

Tectona Grandis ◽

Bark Extract ◽

Phytochemical Screening ◽

Decay Fungi ◽

Wood Decay Fungi

The aim of the research was to examine the antifungal activity and the most effective concentration of teak bark (Tectona grandis Linn F) extract in inhibiting wood decay fungi Schizophyllum commune Fries. Teak bark was derived from BKPH Ledok, Sambong district, Blora Regency, West Java. The Teak bark made into particle with size pass of 40 mesh and retained 60 mesh. Then one kilogram of teak bark particle was maceration with 10 liter of aquadest. The extract then mixes with potatoes dextrose agar (PDA) with several concentration i.e. 0%, 0.25%, 0.5%, 0.75% and 1% and pour into petri dish. One isolate of Schizophyllum commune was put in the center of PDA in each petri dish then incubated for nine days. The bioactive compound of extract teak bark was evaluation used phytochemical screening. Result of the research showed that teak bark extract at concentration 0.25% and 0.5% has antifungal activity values was 27.98% and 40.38% and categorized as medium inhibition growth of S. commune. Meanwhile at concentration 0.75% and 1% the AFA values was 59.94% and 65.51% categorized as strong inhibition growth of S. commune. The bioactive compounds from phytochemical screening test were alkaloid, flavonoid, terpenoid, fenolik, antrakuinon, and saponin. The extract of teak bark with concentration 0.75% and 1% are the most effective concentrations in inhibiting the growth of wood decay fungi S. commune Fries.Keywords : antifungal, phytochemical screening test, Schizophyllum commune, Tectona grandis

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A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI

Revista Árvore ◽

10.1590/1806-908820200000010 ◽

2020 ◽

Vol 44 ◽

Author(s):

Carlos Garrido Pinheiro ◽

Nadia Helena Bianchini ◽

Alana Silveira Pavlack ◽

Marlove Fátima Brião Muniz ◽

Victor Dos Santos Barboza ◽

...

Keyword(s):

Wood Decay ◽

Brown Rot ◽

White Rot ◽

Uv Spectrophotometry ◽

Decay Fungi ◽

Potato Dextrose Agar Medium ◽

Ergosterol Content ◽

Wood Decay Fungi ◽

Antifungal Action ◽

Wet Weight

ABSTRACT Ergosterol is responsible for important functions in the fungal plasma membrane. The influence of fungitoxic agents on membrane ergosterol content is one of the most important mechanisms of antifungal action and its knowledge allows the generation of products that associate active compounds of different mechanisms, consequently improving the effectiveness of wood preservatives. Therefore, this study optimized a method for quantifying ergosterol in wood-decay fungi. The white-rot species selected were Ganoderma applanatum and Trametes versicolor, while the brown-rot were Gloeophyllum trabeum and Lentinus lepideus. Mycelial discs of each species were transferred to Petri dishes containing a cellophane-covered potato-dextrose-agar medium. Mycelia of each fungus were collected, weighed, and transferred to test tubes with 5 mL of 25% alcoholic potassium hydroxide. The tubes were vortexed for 5 min, subjected to ultrasound for 5 min, incubated at 85 °C for 4 h, followed by the addition of 2 mL of sterile distilled water and 5 mL of n-heptane and subsequent ultrasound shaking for 2 min. The n-heptane layer was analyzed by UV spectrophotometry between 230 and 300 ηm. The blank sample only contained n-heptane. The mycelia wet weight of the fungi ranged from 0.061 to 0.296 g. Ergosterol content was 0.007% for Lentinus lepideus and 0.004% for the other species. The absorbance was higher than the ones observed in the blank for all samples. The adapted method was efficient for ergosterol extraction.

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Composition and Antifungal Activities of the Leaf Essential Oil of Neolitsea parvigemma from Taiwan

Natural Product Communications ◽

10.1177/1934578x1100600935 ◽

2011 ◽

Vol 6 (9) ◽

pp. 1934578X1100600

Author(s):

Chen-Lung Ho ◽

Pei-Chun Liao ◽

Eugene I-Chen Wang ◽

Yu-Chang Su

Keyword(s):

Essential Oil ◽

Trichoderma Viride ◽

Wood Decay ◽

Aspergillus Clavatus ◽

Decay Fungi ◽

Wood Decay Fungi ◽

Sesquiterpene Hydrocarbons ◽

Leaf Oil ◽

Leaf Essential Oil ◽

Main Components

The hydrodistillated leaf essential oil of Neolitsea parvigemma was analyzed to determine its composition and yield. Sixty-two compounds were identified, the main components being β-caryophyllene (14.2%), β-eudesmol (12.9%), α-cadinol (10.2%) and τ-cadinol (8.8%). Oxygenated sesquiterpenes (48.9%) and sesquiterpene hydrocarbons (48.8%) were the predominant groups of compounds. The antifungal indexes of the leaf oil against the 7 fungi, Aspergillus clavatus, A. niger, Chaetomium globosum, Cladosporium cladosporioides, Myrothecium verrucaria, Penicillium citrinum and Trichoderma viride, were 100.0, 72.3, 100.0, 100.0, 100.0, 75.8 and 88.6% at a 1 mg/mL concentration, respectively. The oil also exhibited anti-wood-decay-fungi activity against Trametes versicolor, Phaneochaete chrysosporium, Phaeolus schweintizii, and Lenzites sulphureu with MIC values of 50, 50, 25 and 25 μg/mL, respectively. For the antifungal and anti-wood-decay fungal activities of the oil, the active source compounds were determined to be α-cadinol, β-eudesmol and τ-cadinol.

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Structure-activity relationships of cadinane-type sesquiterpene derivatives against wood-decay fungi

Holzforschung ◽

10.1515/hf.2005.100 ◽

2005 ◽

Vol 59 (6) ◽

pp. 620-627 ◽

Cited By ~ 30

Author(s):

Chi-Lin Wu ◽

Shih-Chang Chien ◽

Sheng-Yang Wang ◽

Yueh-Hsiung Kuo ◽

Shang-Tzen Chang

Keyword(s):

Antifungal Activity ◽

Wide Spectrum ◽

Wood Decay ◽

Wood Preservatives ◽

Decay Fungi ◽

Structure Activity Relationships ◽

Chemical Structures ◽

Structure Activity ◽

Wood Decay Fungi

Abstract Cadinane-type sesquiterpenes have a wide spectrum of biological activity, but their use as wood preservatives and the structure-activity relationships of their derivatives have not yet been reported. A total of 13 compounds were synthesized from T-cadinol, T-muurolol, and α-cadinol and their chemical structures were confirmed by IR, MS, and 1H and 13C NMR. The antifungal properties of 16 compounds against three wood-decay fungi were evaluated in vitro. α-Cadinol showed strong antifungal activity against Lenzites betulina, Trametes versicolor, and Laetiporus sulphureus (total mean IC50 0.10 mM). Among the derivatives synthesized, 3β-ethoxy-T-muurolol (0.24 mM), 4ξH-cadinan-10β-ol (0.25 mM), 4ξH-muurolan-10β-ol (0.29 mM), and 4ξH-cadinan-10α-ol (0.25 mM) showed good antifungal activity against all fungi tested. Correlation was observed between the antifungal activity of the compounds tested and log P. Furthermore, the presence of an unsaturated double bond and oxygen-containing functional groups in the compounds plays a key role in their antifungal activity. The stereo configuration of cadinane-type sesquiterpenes also influences their antifungal activity. Understanding how the structure of natural compounds relates to their antifungal function is important and may facilitate their application as novel wood preservatives.

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Composition, in vitro Cytotoxicity, Anti-mildew and Anti-wood-decay Fungal Activities of the Bark Essential Oil of Cunninghamia lanceolata var. konishii from Taiwan

Natural Product Communications ◽

10.1177/1934578x1801300632 ◽

2018 ◽

Vol 13 (6) ◽

pp. 1934578X1801300

Author(s):

Yu-Chang Su ◽

Kuang-Ping Hsu ◽

Chen-Lung Ho

Keyword(s):

Essential Oil ◽

Human Cancer ◽

Wood Decay ◽

In Vitro Cytotoxicity ◽

Cunninghamia Lanceolata ◽

Decay Fungi ◽

Human Cancer Cell Lines ◽

Wood Decay Fungi ◽

Type Apparatus

In this study, cytotoxicity, anti-mildew and anti-wood-decay fungal activities of the bark essential oil and its constituents from Cunninghamia lanceolata var. konishii were evaluated in vitro against three human cancer cell lines, seven mildew fungi, and four wood decay fungi, respectively. The main compounds responsible for the cytotoxicity, anti-mildew, and anti-wood decay fungal activities were also identified. The essential oil from the barks of C. lanceolata var. konishii was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The bark oil mainly consisted of ferruginol (15.1%), β-caryophyllene (11.2%), α-cadinol (10.9%) and cedrol (10.1%). The essential oil exhibited cytotoxic activity against human oral, liver, and lung cancer cells. The active source compounds were α-cadinol, cedrol, β-caryophyllene, and ferruginol. The bark oil was shown to have excellent anti-mildew and anti-wood-decay fungal activities, the active compounds being evaluated as α-cadinol, cedrol, and ferruginol.

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Antifungal activity of phytochemicals fromEucalyptushybrid leaves against some plant pathogenic and wood decay fungi

Archives of Phytopathology and Plant Protection ◽

10.1080/03235408.2012.727073 ◽

2012 ◽

Vol 45 (19) ◽

pp. 2347-2354 ◽

Cited By ~ 2

Author(s):

Vinay Kumar Varshney ◽

Amit Pandey ◽

Praveen Kumar Onial ◽

Rameshwar Dayal

Keyword(s):

Antifungal Activity ◽

Wood Decay ◽

Decay Fungi ◽

Wood Decay Fungi

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Phylogenetic analysis of the genus Fusarium and their antifungal activity against wood-decay and sapstain fungi

Holzforschung ◽

10.1515/hf-2012-0124 ◽

2013 ◽

Vol 67 (4) ◽

pp. 473-478 ◽

Cited By ~ 2

Author(s):

Young Min Lee ◽

Joo-Hyun Hong ◽

Hanbyul Lee ◽

Byoung Jun Ahn ◽

Gyu-Hyeok Kim ◽

...

Keyword(s):

Antifungal Activity ◽

Plant Pathogens ◽

Wood Decay ◽

Decay Fungi ◽

Chemical Treatments ◽

Genus Fusarium ◽

Wood Decay Fungi ◽

Unknown Species ◽

Genotypic Characteristics ◽

First Time

Abstract Microorganism-based biological protections are alternatives to chemical treatments. Worldwide, Fusarium species are important plant pathogens, and their mycotoxins are antimicrobial. In this study, 18 Fusarium strains isolated in Korea have been investigated. Nine Fusarium species, including two unknown species, were identified based on their genotypic characteristics. Among the identified species, Fusarium armeniacum and Fusarium commune were observed for the first time in Korea. The antifungal activity of Fusarium crude extracts was assessed by growth inhibition against two wood-decay fungi and three sapstain fungi. Most Fusarium strain extracts revealed strong antifungal activity against wood-decay fungi. Strains of the unknown Fusarium sp. 2, which was found in wood samples, showed strong antifungal activity against all wood-decay and sapstain fungi tested in this study.

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