Effects of caffeic acid phenethyl ester (CAPE) on angiogenesis, apoptosis and oxidatıve stress ın various cancer cell lines

2019 ◽  
Vol 94 (7) ◽  
pp. 491-497 ◽  
Author(s):  
F. Fırat ◽  
M. Özgül ◽  
E. Türköz Uluer ◽  
S. Inan
Keyword(s):  
Oxidative Stress ◽  
Caffeic Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Caffeic Acid Phenethyl Ester ◽  
And Oxidative Stress

Apoptosis induced by caffeic acid phenethyl ester in human oral cancer cell lines: Involvement of Puma and Bax activation

2017 ◽  
Vol 84 ◽  
pp. 94-99 ◽  
Author(s):  
Hyun-Ju Yu ◽  
Ji-Ae Shin ◽  
In-Hyoung Yang ◽  
Dong-Hoon Won ◽  
Chi Hyun Ahn ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Caffeic Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Caffeic Acid Phenethyl Ester ◽  
Oral Cancer Cell

scholarly journals PROPOLIS OR CAFFEIC ACID PHENETHYL ESTER (CAPE) INHIBITS GROWTH AND VIABILITY IN MULTIPLE ORAL CANCER CELL LINES.

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Whitney Saarem ◽  
Fang Yu Wang ◽  
Elena Farfel
Keyword(s):  
Oral Cancer ◽  
Caffeic Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Normal Cell ◽  
Cancer Cell Lines ◽  
Caffeic Acid Phenethyl Ester ◽  
Cellular Viability ◽  
Cell Control ◽  
Oral Cancer Cell

Objective: Propolis is a natural antimicrobial resin from honeybee hives that contains caffeic acid phenethyl ester (CAPE), which has anti-proliferative activity against some human cancers, including colon, liver, lung and breast – although limited evidence has evaluated this potential in oral cancers. Based upon this information, the primary objective of this study was to evaluate the anti-tumor effects of CAPE against multiple well-characterized oral cancer cell lines. Methods: Using well-characterized oral cancer cell lines (SCC15, SCC25 and CAL27), CAPE was administered at 100 ug/mL to assess any effects on cellular viability or growth over three days. A normal, non-cancerous cell line (HGF-1) was also included. Results: The results of this pilot study demonstrated that CAPE administration significantly reduced both viability and proliferation in all three oral cancer cell lines.  Viability was significantly reduced between 30.3% and 35.4% among the oral cancer cell lines (p<0.05), but remained unchanged in the HGF-1 normal cell control (p=0.878). Growth was significantly inhibited between 53.1% and 60.6% among the oral cancer cell lines (p<0.05) but was not affected in the HGF-1 normal cell control (p=0.341). Conclusions: Although the reductions in both cellular viability and proliferation were distinct for each cell line, all exhibited a similar trend and were within a narrowly defined range. These results strongly suggest that CAPE administration had a significant and immediate effect on oral cancer growth and viability and therefore should be considered as the basis for future studies as a potential complementary and alternative therapy for oral cancer. Key words: Caffeic Acid Phenethyl Ester (CAPE), Propolis, Oral cancer, Complementary and alternative medicine.

scholarly journals Comprehensive Evaluation of Biological Effects of Pentathiepins on Various Human Cancer Cell Lines and Insights into Their Mode of Action

2021 ◽  
Vol 22 (14) ◽  
pp. 7631
Author(s):  
Lisa Wolff ◽  
Siva Sankar Murthy Bandaru ◽  
Elias Eger ◽  
Hoai-Nhi Lam ◽  
Martin Napierkowski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Biological Effects ◽  
Cancer Cell Lines ◽  
Biological Evaluation ◽  
Human Cancer Cell ◽  
Strand Breaks ◽  
Human Cancer Cell Lines

Pentathiepins are polysulfur-containing compounds that exert antiproliferative and cytotoxic activity in cancer cells, induce oxidative stress and apoptosis, and inhibit glutathione peroxidase (GPx1). This renders them promising candidates for anticancer drug development. However, the biological effects and how they intertwine have not yet been systematically assessed in diverse cancer cell lines. In this study, six novel pentathiepins were synthesized to suit particular requirements such as fluorescent properties or improved water solubility. Structural elucidation by X-ray crystallography was successful for three derivatives. All six underwent extensive biological evaluation in 14 human cancer cell lines. These studies included investigating the inhibition of GPx1 and cell proliferation, cytotoxicity, and the induction of ROS and DNA strand breaks. Furthermore, selected hallmarks of apoptosis and the impact on cell cycle progression were studied. All six pentathiepins exerted high cytotoxic and antiproliferative activity, while five also strongly inhibited GPx1. There is a clear connection between the potential to provoke oxidative stress and damage to DNA in the form of single- and double-strand breaks. Additionally, these studies support apoptosis but not ferroptosis as the mechanism of cell death in some of the cell lines. As the various pentathiepins give rise to different biological responses, modulation of the biological effects depends on the distinct chemical structures fused to the sulfur ring. This may allow for an optimization of the anticancer activity of pentathiepins in the future.

Enhancement of 5-Aminolevulinic acid-induced oxidative stress on two cancer cell lines by gold nanoparticles

2009 ◽  
Vol 43 (12) ◽  
pp. 1214-1224 ◽  
Author(s):  
Shinji Ito ◽  
Norio Miyoshi ◽  
William G. Degraff ◽  
Kunio Nagashima ◽  
Louis J. Kirschenbaum ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gold Nanoparticles ◽  
Cell Lines ◽  
Cancer Cell ◽  
Aminolevulinic Acid ◽  
Cancer Cell Lines

scholarly journals Bile acids mimic oxidative stress induced upregulation of thioredoxin reductase in colon cancer cell lines

2002 ◽  
Vol 23 (8) ◽  
pp. 1281-1288 ◽  
Author(s):  
Sandra Lechner ◽  
Ulf Müller-Ladner ◽  
Klaus Schlottmann ◽  
Barbara Jung ◽  
Michael McClelland ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colon Cancer ◽  
Bile Acids ◽  
Cell Lines ◽  
Cancer Cell ◽  
Thioredoxin Reductase ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines

scholarly journals Atg7- and Keap1-dependent autophagy protects breast cancer cell lines against mitoquinone-induced oxidative stress

Oncotarget ◽  
2014 ◽  
Vol 5 (6) ◽  
pp. 1526-1537 ◽  
Author(s):  
Yanira Gonzalez ◽  
Baikuntha Aryal ◽  
Leena Chehab ◽  
V. Ashutosh Rao
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines

scholarly journals Evaluation of the In Vitro Cytotoxic Activity of Caffeic Acid Derivatives and Liposomal Formulation against Pancreatic Cancer Cell Lines

Materials ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 5813
Author(s):  
Magdalena Zaremba-Czogalla ◽  
Anna Jaromin ◽  
Katarzyna Sidoryk ◽  
Agnieszka Zagórska ◽  
Marcin Cybulski ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cytotoxic Activity ◽  
Caffeic Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Pancreatic Cancer Cell ◽  
Cancer Cell Lines ◽  
Liposomal Formulation ◽  
Caffeic Acid Derivatives

Pancreatic cancer belongs to the most aggressive group of cancers, with very poor prognosis. Therefore, there is an important need to find more potent drugs that could deliver an improved therapeutic approach. In the current study we searched for selective and effective caffeic acid derivatives. For this purpose, we analyzed twelve compounds and evaluated their in vitro cytotoxic activity against two human pancreatic cancer cell lines, along with a control, normal fibroblast cell line, by the classic MTT assay. Six out of twelve tested caffeic acid derivatives showed a desirable effect. To improve the therapeutic efficacy of such active compounds, we developed a formulation where caffeic acid derivative (7) was encapsulated into liposomes composed of soybean phosphatidylcholine and DSPE-PEG2000. Subsequently, we analyzed the properties of this formulation in terms of basic physical parameters (such as size, zeta potential, stability at 4 °C and morphology), hemolytic and cytotoxic activity and cellular uptake. Overall, the liposomal formulation was found to be stable, non-hemolytic and had activity against pancreatic cancer cells (IC50 19.44 µM and 24.3 µM, towards AsPC1 and BxPC3 cells, respectively) with less toxicity against normal fibroblasts. This could represent a promising alternative to currently available treatment options.

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