OPTIMAL PRODUCTION OF L-threo-2,3-DIHYDROXYPHENYLSERINE (L-threo-DOPS) ON A LARGE SCALE BY DIASTEREOSELECTIVITY-ENHANCED VARIANT OF L-THREONINE ALDOLASE EXPRESSED INEscherichia coli

2012 ◽  
Vol 42 (2) ◽  
pp. 143-154 ◽  
Author(s):  
Hui-Jeong Gwon ◽  
Hideki Yoshioka ◽  
Nho-Eul Song ◽  
Jong-Hui Kim ◽  
Young-Ran Song ◽  
...  
Keyword(s):  
Large Scale ◽  
Inescherichia Coli ◽  
Optimal Production ◽  
Threonine Aldolase

Analytical redefinition of DOL and managerial investment decisions

2014 ◽  
Vol 40 (7) ◽  
pp. 734-754 ◽  
Author(s):  
Yoram Kroll ◽  
David Yechiam Aharon
Keyword(s):  
Large Scale ◽  
Investment Decision ◽  
Production Level ◽  
Fixed Cost ◽  
Theoretical Formulation ◽  
Optimal Production ◽  
Demand Shocks ◽  
Content Type ◽  
Operating Leverage ◽  
The Impact

Purpose – The purpose of this paper is to develop alternative analytical measures for the degree of operating leverage (DOL) that reflect the impact of uncertain demand shocks in the product's market on optimal production levels, sales and profits of the firm. Design/methodology/approach – The elasticity measures are constructed according to a theoretical formulation of optimal production level that corresponds to demand shocks for given predetermined levels of fixed cost. Findings – The paper suggests two main findings. First, the analytical marginal DOL is at least twice the traditional DOL depending on the structure of the shock, the production function and demand's elasticity. The traditional DOL is equal to the measure only when large-scale negative demand prompts the firm to abandon production. Second, the paper also provides an analytical measure of DOL in terms of elasticity of profit to sales rather than to production level. Both theoretically and empirically elasticity of profit to sales can be better measured and better reflects risk. Research limitations/implications – This paper should be extended to encompass multiple shocks on demand and supply while investigating the empirical multi variants distribution of the shocks. Practical implications – The model can be used by managers who are well informed about the fixed and variable costs of their firm. The model determines the mean profit- risk trade off which is an important factor in all investment decision problems. Originality/value – Surprisingly and according to the best knowledge, this paper is the first attempt in the literature for alternative analytical DOLs’ formulations that is coherent with basic economic theories of optimal production level under risk.

Large-scale purification and characterization of recombinant human stem cell factor inEscherichia coli

2017 ◽  
Vol 64 (4) ◽  
pp. 509-518 ◽  
Author(s):  
Liang-hua Chen ◽  
Feng Cai ◽  
Dan-ju Zhang ◽  
Li Zhang ◽  
Peng Zhu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Large Scale ◽  
Inescherichia Coli ◽  
Human Stem Cell ◽  
Purification And Characterization ◽  
Human Stem Cell Factor

scholarly journals Melon ethylene-mediated transcriptome and methylome dynamics provide insights to volatile production

2020 ◽  
Author(s):  
Ari Feder ◽  
Chen Jiao ◽  
Navot Galpaz ◽  
Julia Vrebalov ◽  
Yimin Xu ◽  
...  
Keyword(s):  
Transcriptional Repression ◽  
Large Scale ◽  
Cytosine Methylation ◽  
Acc Oxidase ◽  
Threonine Aldolase ◽  
Genome Methylation ◽  
Ester Biosynthesis ◽  
Small Set ◽  
Aldolase Activity ◽  
Mutant Complementation

AbstractDuring climacteric ripening large-scale transcriptional modifications are governed by ethylene. While ripening-related chromatin modifications are also known to occur, a direct connection between these factors has not been demonstrated. We characterized ethylene-mediated transcriptome modification, genome methylation dynamics, and their relation to organoleptic modifications during fruit ripening in the climacteric melon and an ethylene repressed line where the fruit-specific ACC oxidase 1 (ACO1) gene was targeted by antisense. The ACO1 antisense line exhibited mainly reduced transcriptional repression of ripening-related genes associated with DNA CHH hypomethylation at the onset of ripening. Additionally, transcription of a small set of ethylene-induced genes, including known ripening-associated genes, was inhibited by ACO1 repression and this inhibition was associated with CG hypermethylation. In the ACO1 antisense line, the accumulation of aromatic compounds, which are mainly derived from the catabolism of amino acids, is known to be inhibited. One of the ethylene-mediated transcriptionally up-regulated genes, CmTHA1, encoding a threonine aldolase, exhibited differential cytosine methylation. Threonine aldolase catalyzes the conversion of L-threonine/L-allo threonine to glycine and acetaldehyde and thus is likely involved in threonine-dependent ethyl ester biosynthesis. Yeast mutant complementation and incubation of melon discs with labeled threonine verified CmTHA1 threonine aldolase activity, revealing an additional ethylene-dependent amino acid catabolism branch involved in climacteric melon ripening.

Some comments about observations and image processing of comet 29P/Schwassmann-Wachmann 1

1999 ◽  
Vol 173 ◽  
pp. 243-248
Author(s):  
D. Kubáček ◽  
A. Galád ◽  
A. Pravda
Keyword(s):  
Image Processing ◽  
Large Scale ◽  
Harvard College ◽  
Oak Ridge ◽  
Large Scale Structures ◽  
Short Period Comet ◽  
Short Period ◽  
Scale Structures ◽  
Harvard College Observatory ◽  
Period Comet

AbstractUnusual short-period comet 29P/Schwassmann-Wachmann 1 inspired many observers to explain its unpredictable outbursts. In this paper large scale structures and features from the inner part of the coma in time periods around outbursts are studied. CCD images were taken at Whipple Observatory, Mt. Hopkins, in 1989 and at Astronomical Observatory, Modra, from 1995 to 1998. Photographic plates of the comet were taken at Harvard College Observatory, Oak Ridge, from 1974 to 1982. The latter were digitized at first to apply the same techniques of image processing for optimizing the visibility of features in the coma during outbursts. Outbursts and coma structures show various shapes.

Evolution of Large-Scale Coronal Structures

1994 ◽  
Vol 144 ◽  
pp. 29-33
Author(s):  
P. Ambrož
Keyword(s):  
Magnetic Field ◽  
Field Line ◽  
Large Scale ◽  
Coronal Magnetic Field ◽  
Source Surface ◽  
Solar Cycles ◽  
Characteristic Relationship ◽  
The Magnetic Field ◽  
Coronal Structures

AbstractThe large-scale coronal structures observed during the sporadically visible solar eclipses were compared with the numerically extrapolated field-line structures of coronal magnetic field. A characteristic relationship between the observed structures of coronal plasma and the magnetic field line configurations was determined. The long-term evolution of large scale coronal structures inferred from photospheric magnetic observations in the course of 11- and 22-year solar cycles is described.Some known parameters, such as the source surface radius, or coronal rotation rate are discussed and actually interpreted. A relation between the large-scale photospheric magnetic field evolution and the coronal structure rearrangement is demonstrated.

Large-scale Motion of Solar Filaments

2000 ◽  
Vol 179 ◽  
pp. 205-208
Author(s):  
Pavel Ambrož ◽  
Alfred Schroll
Keyword(s):  
Magnetic Flux ◽  
Proper Motion ◽  
Time Scale ◽  
Large Scale ◽  
Accuracy Of Measurements ◽  
Solar Filaments ◽  
General Movement ◽  
Scale Motion ◽  
Velocity Scatter

AbstractPrecise measurements of heliographic position of solar filaments were used for determination of the proper motion of solar filaments on the time-scale of days. The filaments have a tendency to make a shaking or waving of the external structure and to make a general movement of whole filament body, coinciding with the transport of the magnetic flux in the photosphere. The velocity scatter of individual measured points is about one order higher than the accuracy of measurements.

Some applications of electron beam microanalysis techniques in VLSI process evaluation

1983 ◽  
Vol 41 ◽  
pp. 160-161
Author(s):  
Simon Thomas
Keyword(s):  
Integrated Circuits ◽  
Process Evaluation ◽  
Large Scale ◽  
Technology Development ◽  
Analytical Techniques ◽  
Successful Implementation ◽  
Analysis Of Failures ◽  
Characterization Of Materials ◽  
Circuits Vlsi

Trends in the technology development of very large scale integrated circuits (VLSI) have been in the direction of higher density of components with smaller dimensions. The scaling down of device dimensions has been not only laterally but also in depth. Such efforts in miniaturization bring with them new developments in materials and processing. Successful implementation of these efforts is, to a large extent, dependent on the proper understanding of the material properties, process technologies and reliability issues, through adequate analytical studies. The analytical instrumentation technology has, fortunately, kept pace with the basic requirements of devices with lateral dimensions in the micron/ submicron range and depths of the order of nonometers. Often, newer analytical techniques have emerged or the more conventional techniques have been adapted to meet the more stringent requirements. As such, a variety of analytical techniques are available today to aid an analyst in the efforts of VLSI process evaluation. Generally such analytical efforts are divided into the characterization of materials, evaluation of processing steps and the analysis of failures.

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