Biological pretreatment of rice straw with Streptomyces griseorubens JSD-1 and its optimized production of cellulase and xylanase for improved enzymatic saccharification efficiency

2016 ◽  
Vol 46 (6) ◽  
pp. 575-585 ◽  
Author(s):  
Dan Zhang ◽  
Yanqing Luo ◽  
Shaohua Chu ◽  
Yuee Zhi ◽  
Bin Wang ◽  
...  
Keyword(s):  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Biological Pretreatment ◽  
Streptomyces Griseorubens ◽  
Saccharification Efficiency

scholarly journals Simultaneous Biological Pretreatment and Saccharification of Rice Straw by Ligninolytic Enzymes from Panus neostrigosus I9 and Commercial Cellulase

2021 ◽  
Vol 7 (10) ◽  
pp. 853
Author(s):  
Ariyah Terasawat ◽  
Sivawan Phoolphundh
Keyword(s):  
Rice Straw ◽  
Continuous Fermentation ◽  
Enzymatic Saccharification ◽  
Ligninolytic Enzymes ◽  
Biofuel Production ◽  
Laccase Production ◽  
Cellulolytic Enzymes ◽  
Biological Pretreatment ◽  
Maximum Percentage ◽  
Enzymatic Accessibility

The utilization of rice straw for biofuel production is limited by its composition. The pretreatment process is required to improve the enzymatic accessibility of polysaccharides in the biomass prior to enzymatic saccharification. In this study, simultaneous biological pretreatment and saccharification (SPS) of rice straw starting from laccase production by Panus neostrigosus I9 was operated in a 2-L fermenter. It was found that fungal physiology was strongly influenced by the agitation, and that the highest laccase production was obtained at an agitation speed of 750 rpm (209.96 ± 0.34 U/L). The dilution rate of 0.05 h−1 was set in continuous fermentation which resulted in laccase activity of 678.49 ± 20.39 U/L, approximately three times higher than that in batch culture. Response surface methodology (RSM) was applied to achieve the condition for maximum percentage of delignification. The maximum percentage of delignification of 45.55% was accomplished after pretreatment of rice straw with laccase enzyme 39.40 U/g rice straw at 43.70 °C for 11.19 h. Reducing sugar of 3.85 ± 0.15 g/L was obtained from the digested rice straw in a SPS reactor, while non-pretreated rice straw gave only 1.13 ± 0.10 g/L within 12 h of incubation. The results indicated that simultaneous biological pretreatment and saccharification (SPS) of rice straw by laccase helped to improve the accessibility of cellulose by cellulolytic enzymes.

scholarly journals A semi-dominant mutation in OsCESA9 improves biomass enzymatic digestibility and salt tolerance by relatively remodeling cell walls in rice

2020 ◽  
Author(s):  
Yafeng Ye ◽  
Shuoxun Wang ◽  
Kun Wu ◽  
Yan Ren ◽  
Hongrui Jiang ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Cellulose Content ◽  
Normal Plant ◽  
Wild Type ◽  
Negative Effects ◽  
Straw Return ◽  
Almost All

Abstract Background: Cellulose synthase (CESA) mutants have potential use in straw processing due to their lower cellulose content, but almost all of the mutants exhibit defective phenotypes in plant growth and development. Balancing normal plant growth with reduced cellulose content remains a challenge, as cellulose content and normal plant growth are typically negatively correlated with one another. Result: Here, the rice (Oryza sativa) semi-dominant brittle culm (sdbc) mutant Sdbc1, which harbors a substitution (D387N) at the first conserved aspartic acid residue of OsCESA9, exhibits lower cellulose content and reduced secondary wall thickness as well as enhanced biomass enzymatic saccharification compared with the wild type (WT). Further experiments indicated that the OsCESA9D387N mutation may compete with the wild-type OsCESA9 for interacting with OsCESA4 and OsCESA7, further forming non-functional or partially functional CSCs. The OsCESA9/OsCESA9D387N heterozygous plants increase salt tolerance through scavenging and detoxification of ROS and indirectly affecting related gene expression. They also improve rice straw return to the field due to their brittle culms and lower cellulose content without any negative effects in grain yield and lodging. Conclusion: Hence, manipulation of OsCESA9D387N can provide the perspective of the rice straw for biofuels and bioproducts due to its improved enzymatic saccharification.

Bioethanol Production from Rice Straw in the MAFF Research Project, Japan

2009 ◽  
Vol 1219 ◽  
Author(s):  
Ken Tokuyasu
Keyword(s):  
Heat Treatment ◽  
Saccharomyces Cerevisiae ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Bioethanol Production ◽  
Chemical Pretreatment ◽  
Simple Method ◽  
Herbaceous Biomass ◽  
Free Glucose ◽  
Simultaneous Saccharification

AbstractRice straw is among the most abundant herbaceous biomass, and regarded as the central feedstock for bioethanol production in Japan. We found that significant amounts of soft carbohydrates (SCs), defined as carbohydrates readily recoverable by mere extraction from the biomass or brief enzymatic saccharification, exist in rice straw in the form of free glucose, free fructose, sucrose, starch, and β-1,3-1,4-glucan. Based on the finding, we proposed a simple method for bioethanol production from rice straw samples with SCs, by a heat treatment for sterilization and starch gelatinization, followed by simultaneous saccharification/fermentation with Saccharomyces cerevisiae. This method would offer an efficient process for bioethanol production without the aid of harsh thermo/chemical pretreatment step.

Evaluation of Fungal Pretreatments for Enzymatic Saccharification of Rice Straw

2010 ◽  
Vol 43 (4) ◽  
pp. 401-405 ◽  
Author(s):  
Masayuki Taniguchi ◽  
Daisuke Takahashi ◽  
Daisuke Watanabe ◽  
Kenji Sakai ◽  
Kazuhiro Hoshino ◽  
...  
Keyword(s):  
Rice Straw ◽  
Enzymatic Saccharification

Sulfite–formaldehyde pretreatment on rice straw for the improvement of enzymatic saccharification

2013 ◽  
Vol 142 ◽  
pp. 218-224 ◽  
Author(s):  
Feng Gu ◽  
Wangxia Wang ◽  
Lei Jing ◽  
Yongcan Jin
Keyword(s):  
Rice Straw ◽  
Enzymatic Saccharification

scholarly journals A semi-dominant mutation in OsCESA9 improves rice straw return to the field and increases salt tolerance by remodeling the cell wall in rice

2020 ◽  
Author(s):  
Yafeng Ye ◽  
Shuoxun Wang ◽  
Kun Wu ◽  
Yan Ren ◽  
Hongrui Jiang ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Cellulose Content ◽  
Normal Plant ◽  
Wild Type ◽  
Negative Effects ◽  
Biomass Saccharification ◽  
Straw Return

Abstract Background Cellulose synthase (CESA) mutants have potential use in straw processing due to their lower cellulose content, but almost all of the mutants exhibit defective phenotypes in plant growth and development. Balancing normal plant growth with reduced cellulose content remains a challenge, as cellulose content and normal plant growth are typically negatively correlated with one another.Result Here, the rice (Oryza sativa) semi-dominant brittle culm (sdbc) mutant Sdbc1, which harbors a substitution (D387N) at the first conserved aspartic acid residue of OsCESA9, exhibits lower cellulose content and reduced secondary wall thickness as well as enhanced biomass enzymatic saccharification compared with the wild type. Further experiments indicated that the OsCESA9D387N mutation may compete with the wild-type OsCESA9 for interacting with OsCESA4 and OsCESA7, further forming non-functional or partially functional CSCs. The OsCESA9/OsCESA9D387N heterozygous plants increase salt tolerance through scavenging and detoxification of ROS and indirectly affecting related gene expression. They also improve rice straw return to the field due to their brittle culms and lower cellulose content without any negative effects in grain yield and lodging.Conclusion Hence, manipulation of OsCESA9D387N can enhance biomass saccharification and simultaneously facilitate the decay of rice straw in the soil, providing a new strategy for bioenergy crop breeding.

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