scholarly journals Effects of Heat Treatment on Total Phenolic Contents, Antioxidant and Anti-Inflammatory Activities ofPleurotus Sajor-CajuExtract

2013 ◽  
Vol 17 (1) ◽  
pp. 219-225 ◽  
Author(s):  
Wan Zuhainis Saad ◽  
Mahfuzatunajla Hashim ◽  
Syahida Ahmad ◽  
Norhani Abdullah
Author(s):  
Rigoberto Villanueva Guerrero ◽  
Rodolfo Abarca Vargas ◽  
Vera L. Petricevich

Objective: A Bougainvillea x buttiana (var. Rose) Holttum and Standl extract (BxbREE) was prepared and its chemical composition, antioxidant and anti-inflammatory activity were evaluated.Methods: For the analyses of the phytochemical compounds present in BxbREE extract, gas chromatography-mass spectrometry (GC/MS) was used. To explore the anti-oxidant, anti-inflammatory activities, total phenolic contents, carbohydrates, lipids and carrageenan-induce paw edema models, respectively, were used. For in vivo experiments, the extract was orally, intraperitoneally and/or subcutaneously administered at doses of 0.04, 0.4, 4 and 40 mg/kg.Results: GC/MS analyses showed the presence of 7 compounds, including 2-Propenoic acid, 3-(2-hydroxyphenyl)-, (E)-(1.19%); 2-Methoxy-4-vinylphenol (0.22%); 3-O-Methyl-d-glucose (92.14%); n-Hexadecanoic acid (0.76%); Hexadecanoic acid, ethyl ester (1.17%); 9,12-octadecadienoic acid, ethyl ester (1.93%); and 9,12,15-Octadecatrienoic acid, ethyl ester (Z,Z,Z) (2.59%). Phytochemical qualitative analysis showed the presence of total phenolic contents at 320 mg of Gallic acid Equivalent/gram of dried extract (GA-Eq/g extract); carbohydrates 5.18 mg/ml and lipids 13.88 mg/ml. In accordance the structures the major compound was 3-O-Methyl-d-glucose. Our results also clearly indicate that BxbREE decreases inflammation in BALB/c mice as a subplantar injection of carrageenan-induced paw edema. The extract presented a potent dose-dependent inhibitory effect. The edema inhibition percentage was significantly lower in groups of animals treated with BxbREE by via intraperitoneal or subcutaneous when compared with those results obtained for groups treated by orally administration (p<0.001).Conclusion: In conclusion, this study established the anti-oxidant and anti-inflammatory activities of Bougainvillea x buttiana (var. Rose); also, this extract could be considered to be a natural anti-oxidant agent that represents an anti-inflammatory remedy.


Author(s):  
Faisal Razzaque ◽  
Ali Sharif ◽  
Bushra Akhtar ◽  
Humaira Majeed Khan ◽  
Muhammad Furqan Akhtar ◽  
...  

Background: Tylophora hirsuta Wall. has long been used as traditional medicine for the treatment of diabetes. Current study is designed to evaluate the antidiabetic and anti-inflammatory activity of different extracts of aerial parts of Tylophora hirsuta. Methods: Sequential maceration was conducted to obtain extracts. Total phenolic contents were determined by FolinCiocalteau method. The antioxidant activity was assessed by DPPH free radical scavenging assay. The extracts were tested for its inhibitory activity against α-amylase in-vitro. In-vivo anti diabetic assay was conducted using alloxan induced diabetic model and OGTT was conducted on normal rats. ELISA was used to determine the proinflammatory cytokines (TNF-α and IL-6). Polyphenolic composition of the extract was analyzed using a HPLC system. Results: Aqueous extract exhibited highest total phenolic contents (985.24± 3.82 mg GAE/100 g DW), antioxidant activity (IC50 = 786.70 ± 5.23 Conclusion: These results showed that Tylophora hirsuta possess strong antidiabetic and anti-inflammatory potentials and justify its folklore use for the management of diabetes.


2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>


Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 106 ◽  
Author(s):  
Oladapo Oluwaseye Olukomaiya ◽  
Chrishanthi Fernando ◽  
Ram Mereddy ◽  
Xiuhua Li ◽  
Yasmina Sultanbawa

Camelina (Camelina sativa) also known as false flax or gold of pleasure is an oilseed crop of the Brassica (Cruciferae) family. Camelina is not a food crop, however, the by-product (meal or cake) obtained from the oil extraction of camelina seeds is useful as animal feed because of its moderate crude protein content. The dietary use of camelina meal in broiler diets is limited to low inclusion due to the presence of anti-nutritional factors such as fibre, phytic acid, glucosinolates and tannins which have negative effects on broiler performance. Solid-state fermentation (SSF) is a suitable processing method for enriching agroindustrial by-products since it offers several cost-effective and practical advantages. In the present study, the effect of SSF on the nutrient composition, phytic acid and total phenolic contents of expeller-extracted camelina meal was evaluated. Aspergillus ficuum (ATCC 66876) was used for SSF under aerobic conditions at 30oC for 7 days. Unfermented and fermented camelina meals were analyzed for dry matter, crude protein, crude fat, crude fibre, total sugar (sucrose) and starch as well as for pH, phytic acid and total phenolic contents. Crude protein was improved by 6.79% while total sugar and starch were reduced by 90.99% and 75.78%, respectively in the solid-state fermented camelina meal. Phytic acid and total phenolic contents were also decreased by 39.17% and 56.11%, respectively. This study revealed that SSF could be used to improve the nutritional quality of camelina meal for improved use in poultry feed formulation.


Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 624
Author(s):  
Laima Česonienė ◽  
Juozas Labokas ◽  
Ina Jasutienė ◽  
Antanas Šarkinas ◽  
Vilma Kaškonienė ◽  
...  

The aim of the study was to evaluate 11 cultivars of blue honeysuckle (Lonicera caerulea L.) for bioactive compounds, antioxidant capacity, and the antibacterial activity of berries. Total phenolic contents (TPCs) and total anthocyanin contents (TACs) were established by using ethanolic extracts. For contents of organic acids and saccharides, aqueous extracts were used, and vitamin C was determined by using oxalic acid solution. DPPH• radical scavenging capacity was evaluated by using ethanolic extracts; antibacterial activity was assessed by using both ethanolic and aqueous extracts. The TPC varied from 364.02 ± 0.41 mg/100 g in ‘Vostorg’ to 784.5 ± 0.3 mg/100 g in ‘Obilnaja’, and TAC ranged from 277.8 ± 1.1 mg/100 g in ‘Čelnočnaja’ to 394.1 ± 8.4 mg/100 g in ‘Nimfa’. Anthocyanins comprised 53.8% of total phenolic contents on average. Among organic acids, citric acid was predominant, averaging 769.41 ± 5.34 mg/100 g, with malic and quinic acids amounting to 289.90 ± 2.64 and 45.00 ± 0.37 mg/100 g on average, respectively. Contents of vitamin C were 34.26 ± 0.25 mg/100 g on average. Organic acids were most effective in the inhibition of both Gram-positive and Gram-negative bacteria tested. In conclusion, berries of L. caerulea are beneficial not only for fresh consumption, but also as a raw material or ingredients of foods with high health-promoting value.


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