Herbal plants produce a diverse range of bioactive molecules, making them a rich source of different types of medicines. Thus, proper scientific evidence or assessment has become the criteria for the acceptance of herbal health claims. Plants-based antioxidants are now preferred to synthetic ones because of safety concerns. There is an increasing interest in natural antioxidants e.g. Polyphenols, present in medicinal and dietary plants, which might help prevent oxidative damage. Natural antioxidant increases the antioxidant capacity of plasma and reduces the risk of disease. As such developing a polyhedral formulation will definitely produce a synergistic effect as needed comparable to standard drugs that are available in the market all over the world. The polyherbal formulation, which has a combination of medicinal herbs such as Zingiber officinale (sukku), Nigella sativa (karunjeeragam), Plectranthus amboinicus (karpooravalli), Pipeer cubeba (Vaal melagu), Trigonella foenum graecum (venthayam) powder was tested for its an antioxidant activity phenolic and alkaloid contents in vitro. The purpose of the present study was to investigate the In-vitro antioxidant phenolic and alkaloid content of polyherbal formulation and its application for treating life-threatening diseases such as cancer, cardiac disease, and diabetes mellitus. In this paper, we report the result of such studies in order to orient future investigations towards the finding of new, potent safe, and easily available food antioxidants.