Secoiridoid glycosides from the fruits of Cornus officinalis

The effects of a Chinese herb, Cornus officinalis, on the motility of human sperm was studied. An aqueous extract was prepared from the dried fruits of the herb and used in this study. The crude extract at a final concentration of 0.5 μg/μl in phosphate buffered saline (pH 7.4) increased sperm motility from 25.8 ± 7.7% to 42.8 ± 10.3% (i.e. 68% increase, n = 7), as determined by the computer-aided-sperm-analysis (CASA) method. The crude extract was fractionated by high-performance liquid chromatography (HPLC) into four fractions: Cl , C2, C3 and C4. Their effects on sperm motility were further studied by CASA. Only the C4 fraction showed substantial stimulatory effects on sperm motility. At a concentration of 5 ng/μl, C4 increased the sperm motility from 15.7 ± 3.8% to 34.5 ± 6.4% (i.e. 120% increase, n = 6) by CASA and from 14.9 ± 4.3 to 28.5 ± 8.1 (i.e. 91% increase, n = 8) by transmembrane migration ratio (TMMR) method. This result suggests that C4 is the active component in Cornus officinalis that enhances sperm motility.

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Two new secoiridoid glycosides from the leaves of Olea europaea L.

Journal of Asian Natural Products Research ◽

10.1080/10286020903310979 ◽

2009 ◽

Vol 11 (11) ◽

pp. 940-944 ◽

Cited By ~ 8

Author(s):

Xiao-Fei Wang ◽

Chen Li ◽

Yan-Ping Shi ◽

Duo-Long Di

Keyword(s):

Olea Europaea ◽

Olea Europaea L ◽

Secoiridoid Glycosides ◽

Olea Europaéa

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Morphological, anatomical and genetic differentiation of Cornus mas, Cornus officinalis and their interspecific hybrid

Dendrobiology ◽

10.12657/denbio.070.005 ◽

2013 ◽

Vol 70 ◽

pp. 45-57 ◽

Cited By ~ 8

Author(s):

Maria Morozowska ◽

Barbara Gawrońska ◽

Agata Woźnicka

Keyword(s):

Genetic Differentiation ◽

Interspecific Hybrid ◽

Cornus Officinalis ◽

Cornus Mas

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Glycogen Phosphorylase-a is a Common Target for Anti-Diabetic Effect of Iridoid and Secoiridoid Glycosides

Journal of Pharmacy & Pharmaceutical Sciences ◽

10.18433/j3fs4f ◽

2013 ◽

Vol 16 (4) ◽

pp. 530 ◽

Cited By ~ 15

Author(s):

Hitesh B Vaidya ◽

Abeer A Ahmed ◽

Ramesh K Goyal ◽

Sukhinder K Cheema

Keyword(s):

Diabetes Mellitus ◽

Medicinal Plants ◽

Glycogen Phosphorylase ◽

Docking Studies ◽

Chemical Components ◽

Active Components ◽

Active Chemical ◽

Treatment Of Diabetes ◽

Secoiridoid Glycosides ◽

Common Target

Purpose. Diabetes mellitus is characterized by hyperglycemia resulting from defects in insulin secretion, action or both. The use of medicinal plants for the treatment of diabetes mellitus dates back from the Ebers papyrus of about 1550 B.C. One of the major problems with herbal drugs is that the active ingredients are not well defined. It is important to know the active components and their molecular interactions which will help to analyze their therapeutic efficacy and also to standardize the product. There are a number of medicinal plants known for their anti-diabetic effect that possess similarities in their active chemical components, e.g. iridoid and secoiridoid glycosides. Methods. In this study, we have compared the structure of various iridoid and secoiridoid glycosides to design a novel pharmacophore. We further developed a structure-activity relationship for the inhibition of glycogen phosphorylase-a. Conclusion. By using docking studies, we are proposing, for the first time, that inhibition of glycogen phosphorylase-a activity is a common target for iridoids and secoiridoids to elicit anti-diabetic effects. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

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Investigation of the Effect of Rice Wine on the Metabolites of the Main Components of Herbal Medicine in Rat Urine by Ultrahigh-Performance Liquid Chromatography-Quadrupole/Time-of-Flight Mass Spectrometry: A Case Study onCornus officinalis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/306712 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10

Author(s):

Gang Cao ◽

Hao Cai ◽

Xianke Yue ◽

Sicong Tu ◽

Baochang Cai ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Herbal Medicine ◽

Rice Wine ◽

Rat Urine ◽

Flight Mass Spectrometry ◽

Cornus Officinalis ◽

Ultrahigh Performance Liquid Chromatography ◽

Main Components

Ultrahigh-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF/MS) was developed for rapid and sensitive analysis of the effect of rice wine on the metabolites of the main components of herbal medicine in rat urine. UsingCornus officinalisas a model of herbal medicine, the metabolite profiles of crude and processed (steaming the crude drug presteeped in rice wine)Cornus officinalisextracts in rat urine were investigated. The metabolites ofCornus officinaliswere identified by using dynamic adjustment of the fragmentor voltage to produce structure-relevant fragment ions. In this work, we identified the parent compounds and metabolites of crude and processedCornus officinalisin rats. In total, three parent compounds and seventeen new metabolites ofCornus officinaliswere found in rats. The contents of the parent compounds and metabolitesin vivovaried significantly after intragastric (i.g.) administration of aqueous extracts of crude and processedCornus officinalis. Data from this study suggests that UPLC-QTOF/MS could be used as a potential tool for uncovering the effects of excipients found in the metabolites of the main components of herbal medicine,in vivo, to predict and discover the processing mechanisms of herbal medicine.

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