Application of Solid Phase Extraction (SPE) Media Rods to Assess Degree of NAPL Encapsulation in In Situ Deposited NAPL Sediments

Solid-phase extraction embedded dialysis (SPEED technology) is an innovative procedure developed to physically separate in-situ, during the cultivation, the mycelium of filament forming microorganisms, such as actinomycetes and fungi, and the XAD-16 resin used to trap the secreted specialized metabolites. SPEED consists of an external nylon cloth and an internal dialysis tube containing the XAD resin. The dialysis barrier selects the molecular weight of the trapped compounds, and prevents the aggregation of biomass or macromolecules on the XAD beads. The external nylon promotes the formation of a microbial biofilm, making SPEED a biofilm supported cultivation process. SPEED technology was applied to the marine Streptomyces albidoflavus 19-S21, isolated from a core of a submerged Kopara sampled at 20 m from the border of a saltwater pond. The chemical space of this strain was investigated effectively using a dereplication strategy based on molecular networking and in-depth chemical analysis. The results highlight the impact of culture support on the molecular profile of Streptomyces albidoflavus 19-S21 secondary metabolites.

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An in situ modification sorbent for magnetic dispersive micro solid-phase extraction of anti-inflammatory drugs in the human urine sample before their determination with high-performance liquid chromatography

Chemical Papers ◽

10.1007/s11696-021-01761-1 ◽

2021 ◽

Author(s):

Zohreh Bagheri Zomoorodi ◽

Mahboubeh Masrournia ◽

Mohamad Reza Abedi

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Solid Phase Extraction ◽

High Performance ◽

Solid Phase ◽

Phase Extraction ◽

In Situ Modification ◽

Human Urine Sample ◽

Inflammatory Drugs

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In Situ Miniaturised Solid Phase Extraction (m-SPE) for Organic Pollutants in Seawater Samples

Journal of Analytical Methods in Chemistry ◽

10.1155/2018/7437031 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

B. Abaroa-Pérez ◽

G. Sánchez-Almeida ◽

J. J. Hernández-Brito ◽

D. Vega-Moreno

Keyword(s):

Solid Phase Extraction ◽

Organic Pollutants ◽

Marine Environment ◽

Persistent Organic Pollutants ◽

Solid Phase ◽

In Situ Monitoring ◽

Phase Extraction ◽

Polycyclic Aromatic ◽

Seawater Samples

Solid phase extraction (SPE) is a consolidated technique for determining pollutants in seawater samples. The current tendency is to miniaturise systems that extract and determine pollutants in the environment, reducing the use of organic solvents, while maintaining the quality in the extraction and preconcentration. On the other hand, there is a need to develop new extraction systems that can be fitted to in situ continual monitoring buoys, especially for the marine environment. This work has developed a first model of a low-pressure micro-SPE (m-SPE) for persistent organic pollutants (POPs) that can be simply applied to in situ monitoring in the marine environment. This system reduces the volumes of sample and solvents required in the laboratory in comparison with conventional SPE. In the future, it could be used in automated or robotic systems in marine technologies such as marine gliders and oceanographic buoys. This system has been optimised and validated to determine polycyclic aromatic hydrocarbons (PAH) in seawater samples, but it could also be applied to other kinds of persistent organic pollutants (POPs) and emerging pollutants.

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