Protective effect of Fragaria ananassa methanolic extract on cadmium chloride (CdCl2)-induced hepatotoxicity in rats

2017 ◽  
Vol 27 (5) ◽  
pp. 335-345 ◽  
Author(s):  
Manal F. Elkhadragy ◽  
Ahmed E. Abdel Moneim
Keyword(s):  
Protective Effect ◽  
Cadmium Chloride ◽  
Methanolic Extract ◽  
Fragaria Ananassa

scholarly journals Protective effects of Fragaria ananassa methanolic extract in a rat model of cadmium chloride-induced neurotoxicity

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Manal F. Elkhadragy ◽  
Rami B. Kassab ◽  
Dina Metwally ◽  
Rafa S. Almeer ◽  
Rewaida Abdel-Gaber ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Glutathione Peroxidase ◽  
Brain Tissue ◽  
Cadmium Chloride ◽  
Methanolic Extract ◽  
Oxidative Enzymes ◽  
Protective Effects ◽  
Fragaria Ananassa ◽  
Neuronal Toxicity ◽  
The Brain

Cadmium (Cd) is a common environmental toxicant that has harmful effects on plants, animals, and humans. The present study evaluated the protective effects of Fragaria ananassa methanolic extract (SME) on cadmium chloride (CdCl2)-induced neuronal toxicity in rats. Male albino rats were intraperitoneally (i.p) injected with CdCl2 (6.5 mg/kg) for 5 days with or without the SME (250 mg/kg). We measured the levels of Cd, lipid peroxidation (LPO), nitric oxide, glutathione (GSH), and oxidative enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase, and glutathione reductase (GR) in the whole brain homogenate. Compared with the control group, the Cd-intoxicated group showed a marked increase in the brain levels of Cd, LPO, and nitric oxide and a decrease in the levels of GSH and all tested antioxidant enzymes. Compared with Cd-intoxicated rats, the rats pretreated with SME showed restoration of oxidative balance in the brain tissue. While the expression of brain SOD2, CAT, glutathione peroxidase 1, and GR was down-regulated in the Cd-treated group, the expression of these enzymes was up-regulated in rats pretreated with SME. In addition, administration of SME before CdCl2 increased the Bcl-2 expression, but significantly decreased the expression of Bax. Immunohistochemical analysis showed that compared with Cd-intoxicated rats, rats pretreated with SME showed a decrease in the protein expression of tumor necrosis factor α (TNF-α). Our findings indicate that SME protects the brain tissue from Cd-induced neuronal toxicity by improving the antioxidant system and increasing antiapoptotic and anti-inflammatory activities.

scholarly journals Protective effect of butylated hydroxytoluene (BHT) against the clastogenic acitivity of cadmium chloride and potassium dichromate in hamster ovary cells

1999 ◽  
Vol 22 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Claudia A. Grillo ◽  
Analía I. Seoane ◽  
Fernando N. Dulout
Keyword(s):  
Protective Effect ◽  
Chromosomal Aberration ◽  
Cho Cells ◽  
Cadmium Chloride ◽  
Potassium Dichromate ◽  
Chinese Hamster ◽  
Butylated Hydroxytoluene ◽  
Chromosomal Damage ◽  
Combined Treatments ◽  
Chromatin Bridges

The effect of butylated hydroxytoluene (BHT), a widely used food additive, on chromosomal alterations induced by cadmium chloride (CC) and potassium dichromate (PD) in Chinese hamster ovary (CHO) cells was studied both at metaphase and anaphase-telophase. CHO cells were cultured for 15-16 h in the presence of PD (6.0, 9.0 or 12.0 <FONT FACE="Symbol">m</font>M), BHT (1.0 <FONT FACE="Symbol">m</font>g/ml), or PD plus BHT as well as CC (0.5, 1.0 and 2.0 <FONT FACE="Symbol">m</font>M), BHT or CC plus BHT for the analysis of chromosomal aberrations. To perform the anaphase-telophase test, cells were cultured in cover glasses and treated 8 h before fixation with the same chemicals. An extra dose of CC (4 <FONT FACE="Symbol">m</font>M) was used in this test. Both metal salts significantly increased chromosomal aberration frequencies in relation to untreated controls, and to DMSO- and BHT-treated cells. Post-treatment with BHT decreased the yield of chromosomal damage in relation to treatments performed with CC and PD. However, chromosomal aberration frequencies were significantly higher than those of the controls. In the anaphase-telophase test, CC significantly increased the yield of lagging chromosomes with the four doses employed and the frequency of lagging fragments with the highest dose. In combined treatments of CC and BHT, frequencies of the two types of alterations decreased significantly in relation to the cells treated with CC alone. No significant variation was found in the frequencies of chromatin bridges. Significant increases of numbers of chromatin bridges, lagging chromosomes and lagging fragments were found in cells treated with PD. The protective effect of BHT in combined treatments was evidenced by the significant decrease of chromatid bridges and lagging chromosomes in relation to PD-treated cells. Whereas BHT is able to induce chromosomal damage, it can also protect against oxidative damage induced by other genotoxicants.

Protective Effect of Grape and Apple Juices against Cadmium Intoxication in the Kidney of Rats

Drug Research ◽  
2020 ◽  
Vol 70 (11) ◽  
pp. 503-511
Author(s):  
Bianca Andrade Handan ◽  
Carolina Foot Gomes De Moura ◽  
Caroline Margonato Cardoso ◽  
Aline Boveto Santamarina ◽  
Luciana Pellegrini Pisani ◽  
...  
Keyword(s):  
Protective Effect ◽  
Cadmium Chloride ◽  
Apple Juice ◽  
Tissue Injury ◽  
Grape Juice ◽  
Control Group ◽  
Histopathological Analysis ◽  
Gene Expressions ◽  
Cadmium Intoxication ◽  
Male Wistar Rats

Abstract Background The objective of this study was to evaluate protective effect of grape and apple juices against toxicity induced by cadmium in the kidney of rats. Methods A total of 20 male-Wistar rats were distributed into four groups (n=5): Control group: animals received an intraperitoneal (i.p.) injection of 0.9% saline solution and after 15 days, 1 mL of water was administered for 15 days, via gavage; Cadmium group: animals received an intraperitoneal injection of cadmium chloride (1.2 mg/kg) and after 15 days, 1 mL of water was administered for 15 days via gavage; Cadmium+Grape Juice: animals received an i.p. injection of cadmium chloride (1.2 mg/kg), and after 15 days, 0.8 mL of grape juice was administered for 15 days, via gavage; Cadmium+Apple Juice: animals received i.p. injection of cadmium chloride (1.2 mg/kg) and after 15 days, 1.0 mL of apple juice was administered for 15 days, via gavage. Results Histopathological analysis revealed severe tubular lesion and necrosis in the group exposed to cadmium, while animals exposed to grape or apple juices showed a significant reduction of tissue injury. 8-OHdG immunoexpression, DNA damage, cytochrome C and catalase gene expressions and Toll like signaling pathway (TLR2, and pIKKα/β) decreased in animals treated with grape juice when compared to cadmium group. Conclusion Taken together, we conclude that grape and apple juices had a protective effect by means of antioxidant, antigenotoxic actions and for promoting tissue regeneration in the kidney of rats following cadmium intoxication.

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