Antioxidant, cytotoxicity, anti-human esophageal squamous cell carcinoma, anti-human Caucasian esophageal carcinoma, anti-adenocarcinoma of the gastroesophageal junction, and anti-distal esophageal adenocarcinoma properties of gold nanoparticles green synthesized by Rhus coriaria L. fruit aqueous extract

The high incidence of esophageal squamous cell carcinoma has been reported in selected ethnic populations including North of Iran. Low survival rate of esophageal carcinoma is partially due to the presence of stem-like cancer cells with chemotherapy resistance. In the current study, we aimed to determine the effects of auraptene, an interesting dietary coumarin with various biological activities, on malignant properties of stem-like esophageal squamous cell carcinoma, in terms of sensitivity to anticancer drugs and expression of specific markers. To do so, the half maximal inhibitory concentration values of auraptene, cisplatin, paclitaxel, and 5-fluorouracil were determined on esophageal carcinoma cells (KYSE30 cell line). After administrating combinatorial treatments, including nontoxic concentrations of auraptene + cisplatin, paclitaxel, or 5-fluorouracil, sensitivity of cells to chemical drugs and also induced apoptosis were assessed. In addition, quantitative real-time polymerase chain reaction was used to study changes in the expression of tumor suppressor proteins 53 and 21 ( P53 and P21), cluster of differentiation 44 ( CD44), and B cell-specific Moloney murine leukemia virus integration site 1 ( BMI-1) upon treatments. Results of thiazolyl blue assay revealed that auraptene significantly ( P < .05) increased toxicity of cisplatin, paclitaxel, and 5-fluorouracil in KYSE30 cells, specifically 72 hours after treatment. Conducting an apoptosis assay using flow cytometry also confirmed the synergic effects of auraptene. Results of quantitative real-time polymerase chain reaction revealed significant ( P < .05) upregulation of P53 and P21 upon combinatorial treatments and also downregulation of CD44 and BMI-1 after auraptene administration. Current study provided evidence, for the first time, that auraptene attenuates the properties of esophageal stem-like cancer cells through enhancing sensitivity to chemical agents and reducing the expression of CD44 and BMI-1 markers.

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Expressions of HPV 16-E6 in esophageal carcinoma and it's clinical significance

Asian Journal of Medical Sciences ◽

10.3126/ajms.v6i6.12537 ◽

2015 ◽

Vol 6 (6) ◽

pp. 39-42

Author(s):

Xing Zhao ◽

Rajina Sahi ◽

Yu-Yang Zhao ◽

Jun Wang ◽

Chun- Hui Li

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Immunohistochemical Method ◽

Esophageal Mucosa ◽

Medical Sciences ◽

Hpv 16 ◽

Hpv16 E6

Aim: To explore the association between HPV16-E6 protein and esophageal squamous cell carcinoma.Materials and Methods: SP immunohistochemical method was used to examine the expression of HPV 16-E6 in 50 cases of esophageal squamous cell carcinoma, 10 cases of normal esophageal squamous cell and 10 cases of adjacent tissue.Results: The expressions of HPV 16-E6 was significantly higher in esophageal carcinoma than in normal esophageal mucosa and in adjacent tissue. The expressions of HPV 16-E6 had correlation with invasive depth (P<0.05), but not with patient age, lymph node metastasis, tumor size (P>0.05).Conclusion: HPV 16-E6 can promote the growth and metastasis of esophageal squamous cell carcinoma and can be a prognostic factor of esophageal squamous cell carcinoma. DOI: http://dx.doi.org/10.3126/ajms.v6i6.12537 Asian Journal of Medical Sciences Vol.6(6) 2015 39-42

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Protein-coding genes combined with long non-coding RNAs predict prognosis in esophageal squamous cell carcinoma patients as a novel clinical multi-dimensional signature

Molecular BioSystems ◽

10.1039/c6mb00585c ◽

2016 ◽

Vol 12 (11) ◽

pp. 3467-3477 ◽

Cited By ~ 15

Author(s):

Jin-Cheng Guo ◽

Chun-Quan Li ◽

Qiu-Yu Wang ◽

Jian-Mei Zhao ◽

Ji-Yu Ding ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Mortality Rate ◽

Esophageal Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Gastrointestinal Cancers ◽

Protein Coding ◽

Protein Coding Genes ◽

Non Coding Rnas

Esophageal carcinoma is one of the most malignant gastrointestinal cancers worldwide, and has a high mortality rate.

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Preliminary evaluation for Bit1 as a potential biomarker for squamous cell carcinoma and adenocarcinoma of esophagus

Tumor Biology ◽

10.1177/1010428317708267 ◽

2017 ◽

Vol 39 (5) ◽

pp. 101042831770826

Author(s):

Jing Chen ◽

Hongtao Liu ◽

Pan Gao ◽

Yiran Hui ◽

Zhenzhen Yang ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Matrix Metalloproteinase ◽

Cell Carcinoma ◽

Esophageal Adenocarcinoma ◽

Squamous Cell ◽

Expression Patterns ◽

Matrix Metalloproteinase 2 ◽

Potential Biomarker ◽

Well Differentiated

Mounting evidence has demonstrated that Bit1 has been investigated as an etiological factor for certain cancers, including esophageal squamous cell carcinoma reported in our previous study, but data regarding possible roles of Bit1 in esophageal squamous cell carcinoma and esophageal adenocarcinoma remain to be elucidated. The purpose of this study was to examine whether Bit1 can be a novel diagnostic marker for the patients with esophageal squamous cell carcinoma and esophageal adenocarcinoma. The results revealed that Bit1 level in esophageal squamous cell carcinoma was significantly higher than that in esophageal adenocarcinoma tissues ( p < 0.05); notably, Bit1 level in esophageal adenocarcinoma tissues was lower than that in paired normal tissues but no difference was found ( p > 0.05). Bit1 expression patterns were completely in accordance with matrix metalloproteinase 2 and Bcl-2 in esophageal squamous cell carcinoma and esophageal adenocarcinoma. In addition, Bit1, Bcl-2, and matrix metalloproteinase 2 expression patterns in different differentiated esophageal squamous cell carcinoma were higher than those in corresponding normal esophageal tissues. Bit1 expression in poorly differentiated esophageal squamous cell carcinoma was significantly higher than that in normal esophageal tissues ( p < 0.05) but not in moderately and well-differentiated esophageal squamous cell carcinoma. Matrix metalloproteinase 2 expression patterns in poorly and moderately differentiated esophageal squamous cell carcinoma were significantly higher than those in corresponding normal esophageal tissues ( p < 0.01) but not in well-differentiated esophageal squamous cell carcinoma tissue ( p > 0.05). Bcl-2 expression patterns in various differentiated esophageal squamous cell carcinoma were higher than those in corresponding normal esophageal tissues with no statistical differences ( p > 0.05). Importantly, Bit1 expression was positively correlated with both matrix metalloproteinase 2 and Bcl-2 expression in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissues ( p < 0.05). Collectively, these preliminary data support further investigation of Bit1 as an important diagnostic factor for esophageal squamous cell carcinoma and esophageal adenocarcinoma.

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