Expressions of HPV 16-E6 in esophageal carcinoma and it's clinical significance

Aim: To explore the association between HPV16-E6 protein and esophageal squamous cell carcinoma.Materials and Methods: SP immunohistochemical method was used to examine the expression of HPV 16-E6 in 50 cases of esophageal squamous cell carcinoma, 10 cases of normal esophageal squamous cell and 10 cases of adjacent tissue.Results: The expressions of HPV 16-E6 was significantly higher in esophageal carcinoma than in normal esophageal mucosa and in adjacent tissue. The expressions of HPV 16-E6 had correlation with invasive depth (P<0.05), but not with patient age, lymph node metastasis, tumor size (P>0.05).Conclusion: HPV 16-E6 can promote the growth and metastasis of esophageal squamous cell carcinoma and can be a prognostic factor of esophageal squamous cell carcinoma. DOI: http://dx.doi.org/10.3126/ajms.v6i6.12537 Asian Journal of Medical Sciences Vol.6(6) 2015 39-42

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Expressions of HPV 16-E6 in esophageal carcinoma and it’s clinical significance

Journal of Pathology of Nepal ◽

10.3126/jpn.v5i9.13786 ◽

2015 ◽

Vol 5 (9) ◽

pp. 752-755

Author(s):

Zhao Xing ◽

R Sahi ◽

Zhao Yu-Yang ◽

Wang Jun ◽

Chun-Hui Li

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Immunohistochemical Method ◽

Esophageal Mucosa ◽

Hpv 16 ◽

Hpv16 E6 ◽

E6 Protein

Background?Gastrointestinal cancer is the most common malignant tumor in China. This research aims to explore the association between HPV16-E6 protein and esophageal squamous cell carcinoma.Materials and Methods: SP immunohistochemical method was used to examine the expression of HPV 16-E6 in 50 cases of esophageal squamous cell carcinoma, 10 cases of normal esophageal squamous cell and 10 cases of adjacent tissue.Results: The expressions of HPV 16-E6 was significantly higher in esophageal carcinoma than in normal esophageal mucosa and in adjacent tissue. The expressions of HPV 16-E6 had correlation with invasive depth (P<0.05), but not with patient age, lymph node metastasis, tumor size (P>0.05).Conclusion: HPV 16-E6 can promote the growth and metastasis of esophageal squamous cell carcinoma and can be a prognostic factor of esophageal squamous cell carcinoma.Journal of Pathology of Nepal (2015) Vol. 5, 752-755

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Expressions of HPV 16-E6 in esophageal carcinoma and its clinical significance

Journal of Advances in Internal Medicine ◽

10.3126/jaim.v4i2.16897 ◽

2017 ◽

Vol 4 (2) ◽

pp. 57-60

Author(s):

Xing Zhao ◽

Rajina Sahi ◽

Yu-Yang Zhao ◽

Jun Wang ◽

Chun-Hui Li

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Immunohistochemical Method ◽

Esophageal Mucosa ◽

Hpv 16 ◽

Hpv16 E6 ◽

E6 Protein

Aim: The aim of the study was to explore the association between HPV16-E6 protein and esophageal squamous cell carcinoma.Methods: SP immunohistochemical method was used to examine the expression of HPV 16-E6 in 50 cases of esophageal squamous cell carcinoma, 10 cases of normal esophageal squamous cell and 10 cases of adjacent tissue.Result: The expressions of HPV 16-E6 was significantly higher in esophageal carcinoma than in normal esophageal mucosa and in adjacent tissue. The expressions of HPV 16-E6 had a significant correlation with invasive depth (P<0.05), but not with the patient age, lymph node metastasis and tumor size (P>0.05).Conclusion: HPV 16-E6 can promote the growth and metastasis of esophageal squamous cell carcinoma and can be a prognostic factor of esophageal squamous cell carcinoma.

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Expressions of HPV 16-E6 in Esophageal Carcinoma and its Clinical Significance

Journal of College of Medical Sciences-Nepal ◽

10.3126/jcmsn.v10i4.12970 ◽

2015 ◽

Vol 10 (4) ◽

pp. 1-5

Author(s):

Xing Zhao ◽

Rajina Sahi ◽

Yu-Yang Zhao ◽

Ju Wang ◽

Chun-Hui Li

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Immunohistochemical Method ◽

Anatomical Location ◽

Esophageal Mucosa ◽

Hpv 16 ◽

E6 Protein

Background and Objective: The role of (Human Papilloma Virus) HPV in cancer of certain anatomical location, such as cervix, has been widely recognized. The present study was conducted to explore the association between HPV 16-E6 protein and esophageal squamous cell carcinoma.Methods: SP immunohistochemical method was used to examine the expression of HPV 16-E6 in 50 cases of esophageal squamous cell carcinoma, 10 cases of normal esophageal squamous cell and 10 cases of adjacent tissue.Results: The expression of HPV 16-E6 was significantly higher in esophageal carcinoma than in normal esophageal mucosa and in adjacent tissue. The expressions of HPV 16-E6 had significant correlation with invasive depth (P<0.05), but not with patient age, lymph node metastasis, tumor size (P>0.05).Conclusion: HPV 16-E6 can promote the growth and metastasis of esophageal squamous cell carcinoma and can be a prognostic factor of esophageal squamous cell carcinoma.JCMS Nepal 2014; 10(4):1-5

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PS02.048: DECREASED EXPRESSION OF PRSS27 IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA

Diseases of the Esophagus ◽

10.1093/dote/doy089.ps02.048 ◽

2018 ◽

Vol 31 (Supplement_1) ◽

pp. 134-134

Author(s):

Masayoshi Terayama ◽

Teruki Hagiwara ◽

Kazuhiko Yamada ◽

Daisuke Soma ◽

Kyoko Nohara ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Clinical Features ◽

Squamous Cell ◽

Cell Layer ◽

Normal Mucosa ◽

Esophageal Mucosa ◽

Gene And Protein Expression ◽

Significant Difference

Abstract Background Alcohol drinking and smoking are substantial risk factors of esophageal squamous cell carcinoma (ESCC) and are supposed to induce genetic mutations and epigenetic disorders, including aberrant DNA methylation. Previously, we have conducted transcriptome and methylome analyses of a paired specimen of ESCC and adjacent non-cancerous tissues and found that both gene expression and promotor methylation of PRSS27 were perturbed in ESCC. PRSS27 was a trypsin-like serine protease (also known as marapsin) and expressed in normal esophagus; however, little is known about the significance of PRSS27 expression in ESCC. In this study, we evaluated the expression of PRSS27 in many ESCC cases in relation with clinical features and the prognosis. Methods ESCC tissue specimens were obtained from 80 patients who had undergone esophagectomy between April 2008 and December 2016 in our hospital and were subjected to immunostaining for PRSS27. ESCC cases were classified into PRSS27-negative and PRSS27-positive groups and difference of clinical features and the prognosis between the groups was analyzed. Results The mRNA expression of PRSS27 was significantly decreased in ESCC compared with those in matched normal mucosa (P < 0.0001). Histologically, PRSS27 was highly expressed in spinous cells of suprabasal cell layer but not in basal cell layer of normal esophageal mucosa. In contrast, 37 of 80 (47%) ESCC exhibited decreased intensity of PRSS27 staining when compared with that in normal mucosa, and 53% (43/80) of ESCC showed almost no staining of PRSS27. Although the prognosis of PRSS27-positive cases were worse in trend than that of PRSS27-negative cases, there was no significant difference (P = 0.0763) Conclusion PRSS27 gene and protein expression was both downregulated in ESCC; its functional significance in relation to malignancy is underinvestigation. Disclosure All authors have declared no conflicts of interest.

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Auraptene Attenuates Malignant Properties of Esophageal Stem-Like Cancer Cells

Technology in Cancer Research & Treatment ◽

10.1177/1533034616650119 ◽

2016 ◽

Vol 16 (4) ◽

pp. 519-527 ◽

Cited By ~ 10

Author(s):

Saffiyeh Saboor-Maleki ◽

Fatemeh B. Rassouli ◽

Maryam M. Matin ◽

Mehrdad Iranshahi

Keyword(s):

Polymerase Chain Reaction ◽

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Esophageal Carcinoma ◽

Cell Carcinoma ◽

Cancer Cells ◽

Real Time ◽

Squamous Cell ◽

Chain Reaction ◽

Polymerase Chain

The high incidence of esophageal squamous cell carcinoma has been reported in selected ethnic populations including North of Iran. Low survival rate of esophageal carcinoma is partially due to the presence of stem-like cancer cells with chemotherapy resistance. In the current study, we aimed to determine the effects of auraptene, an interesting dietary coumarin with various biological activities, on malignant properties of stem-like esophageal squamous cell carcinoma, in terms of sensitivity to anticancer drugs and expression of specific markers. To do so, the half maximal inhibitory concentration values of auraptene, cisplatin, paclitaxel, and 5-fluorouracil were determined on esophageal carcinoma cells (KYSE30 cell line). After administrating combinatorial treatments, including nontoxic concentrations of auraptene + cisplatin, paclitaxel, or 5-fluorouracil, sensitivity of cells to chemical drugs and also induced apoptosis were assessed. In addition, quantitative real-time polymerase chain reaction was used to study changes in the expression of tumor suppressor proteins 53 and 21 ( P53 and P21), cluster of differentiation 44 ( CD44), and B cell-specific Moloney murine leukemia virus integration site 1 ( BMI-1) upon treatments. Results of thiazolyl blue assay revealed that auraptene significantly ( P < .05) increased toxicity of cisplatin, paclitaxel, and 5-fluorouracil in KYSE30 cells, specifically 72 hours after treatment. Conducting an apoptosis assay using flow cytometry also confirmed the synergic effects of auraptene. Results of quantitative real-time polymerase chain reaction revealed significant ( P < .05) upregulation of P53 and P21 upon combinatorial treatments and also downregulation of CD44 and BMI-1 after auraptene administration. Current study provided evidence, for the first time, that auraptene attenuates the properties of esophageal stem-like cancer cells through enhancing sensitivity to chemical agents and reducing the expression of CD44 and BMI-1 markers.

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HPV16 E6-E7 Induces Cancer Stem-like Cell Phenotypes and Chemoradioresistance in Esophageal Squamous Cell Carcinoma Through Activation of PI3K/Akt Signaling Pathway

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/j.ijrobp.2016.06.2027 ◽

2016 ◽

Vol 96 (2) ◽

pp. E559

Author(s):

S. Che ◽

R. Xi ◽

S. Pan ◽

X. Chen ◽

B. Hui ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Signaling Pathway ◽

Squamous Cell ◽

Akt Signaling ◽

Akt Signaling Pathway ◽

Hpv16 E6 ◽

Cell Phenotypes

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HPV Infection and its Relationship with Expression of PIK3CA, PIK3CB in Esophageal Squamous Cells Carcinoma

10.21203/rs.3.rs-502500/v1 ◽

2021 ◽

Author(s):

Feifei Wen ◽

Yangyang Li ◽

Shuang He ◽

Xiaoyang Xu ◽

Ningjie Guo ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Hpv Infection ◽

Tumor Location ◽

Esophageal Mucosa ◽

Squamous Cells ◽

Hpv Status

Abstract Background: Esophageal squamous cell carcinoma (ESCC) is a highly lethal cancer. Human papillomavirus (HPV) is currently considered as a potential risk factor for ESCC, but this assumption is still contradictory. P16INK4a (p16) staining can be used as an alternative marker of HPV oncogene activity. This study was to investigate the role of HPV in esophageal cancer and to compare the relationship between HPV status and PIK3CA, PIK3CB. Methods: Immunohistochemistry, Western blot, and RT-PCR analyses were performed to detect the expression of p16, PIK3CA, PIK3CB and p53 in 156 cases of esophageal squamous cell carcinoma. The patients were followed up by telephone or clinic. Results: The positive rates of p16, PIK3CA, PIK3CB and p53 in esophageal squamous cell carcinoma were significantly higher than those in normal esophageal mucosa. The overexpression of p16 was closely related to tumor location, TNM stage, differentiation and lymph node metastasis. In addition, the expression of p16 was positively correlated with the expression of PIK3CA, but not with the expression of PIK3CB and p53. Survival analysis showed that p16 was a good prognostic marker, while PIK3CA and p53 were poor prognostic markers. Conclusions: HPV infection is associated with ESCC. The imbalance of PI3K/Akt signaling pathway is closely related to HPV infection and prognosis. Detection of p16 and PIK3CA is of great significance in evaluating the prognosis and optimizing the treatment of esophageal squamous cell carcinoma.

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Early Esophageal Squamous Cell Carcinoma and Precancerous Lesions can be Detected by white Light Endoscopy with the Aid of White Substance

10.21203/rs.3.rs-37996/v1 ◽

2020 ◽

Author(s):

Yingbang Wang ◽

Guodong Yang ◽

Xiaoying Zhang ◽

Xiaoming Zhang ◽

Linyi Wu ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Precancerous Lesions ◽

Diagnostic Value ◽

Esophageal Mucosa ◽

Neoplastic Lesions ◽

The Difference ◽

White Substance

Abstract Previous studies have shown that the presence of white substance on the esophageal mucosa is associated with tumor lesions of the flat esophageal mucosa. Since the early lesions of esophageal mucosa are mostly flat, we reasoned that the white substance might also be indicative of early esophageal squamous cell carcinoma and precancerous lesions. Objective: To investigate the diagnostic value of white substance in the detection of flat esophageal mucosal lesions and precancerous lesions associated with early esophageal squamous cell carcinoma Methods: Clinical and pathological data of patients diagnosed with flat esophageal mucosal disease were collected. The lesions were divided into a neoplastic group and a non-neoplastic group, and the clinicopathological differences between the groups were analyzed. The patients were also divided into two groups based on the presence of white substance after endoscopic examination: a “white substance” group and a “non-white substance” group. The differences between the two groups were analyzed. The diagnostic value of white substance for detection of early esophageal squamous cell carcinoma and related precancerous lesions was calculated and the pathological nature of white substance inferred. Results: In total, 183 patients with flat esophageal mucosal lesions were enrolled, including 92 (50.3%) with neoplastic lesions and 91 (49.7%) with non-neoplastic lesions. Forty-nine cases (26.8%) presented white substance in the esophageal mucosa. White substance was mainly found in female patients (57.1%), middle esophagus (75.5%), and 60-69-year-old patients (51.0%). Moreover, white substance was more frequently found in neoplastic than in non-neoplastic lesions (43.5% vs. 11.0%, p < 0.05). Consistently, neoplastic lesions were more represented in the white substance group than in the non-white substance group and the difference was statistically significant (79.6 vs. 20.4%, p < 0.05). 51% of the patients in the white substance group, but only 26.1% of those in the non-white substance group, had hyperkeratosis and necrosis, and the difference was statistically significant (p < 0.05). The diagnostic sensitivity of white substance for detection of early esophageal squamous cell carcinoma and precancerous lesions was 42.4% (95% CI, 32.8%–52.6%),the specificity 89.0% (95% CI, 80.8%–94.1%).Conclusions: White substance in the mucosa of flat esophageal lesions exhibited high specificity for the diagnosis of early esophageal squamous cell carcinoma and precancerous lesions. Thus, the presence of white substance in the esophageal mucosa, even in the absence of obvious lesions, may reflect the presence of latent early esophageal squamous cell carcinoma and precancerous lesions. White substance was associated with hyperkeratosis and necrosis.

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