Methane production and microbial protein synthesis in adult sheep fed total mixed ration as mash and as complete feed block

2019 ◽  
Vol 10 (3) ◽  
pp. 241-253 ◽  
Author(s):  
Randhir Singh Bhatt ◽  
Lalit Soni ◽  
Artabandhu Sahoo
2019 ◽  
Vol 59 (9) ◽  
pp. 1674 ◽  
Author(s):  
Metha Wanapat ◽  
Thiwakorn Ampapon ◽  
Kampanat Phesatcha ◽  
Sungchhang Kang

Replacement of chemical compounds by dietary sources as rumen enhancers have been of great interest and concern by researchers. Four, rumen-fistulated swamp buffalo bulls with average liveweight of 365 ± 15.0 kg were randomly assigned to treatments, to investigate the impact of banana flower powder (BAFLOP) as a rumen modifier on pH, rumen fermentation, nutrient digestibility, microbial protein synthesis and volatile fatty acids. All buffaloes were allotted according to a 4 × 4 Latin square design. Dietary supplementation treatments were as follows: 2 g concentrate/kg bodyweight (BW; T1), 15 g concentrate/kg BW (T2), 15 g concentrate/kg BW plus BAFLOP 300 g/head.day (T3) and 15 g concentrate/kg BW plus BAFLOP 600 g/head.day (T4). Untreated rice straw was fed ad libitum. The findings showed that total feed intake was increased in buffaloes fed a diet supplemented with concentrate at 2 g/kg BW, while rice straw intake was reduced. Nutrient digestibility was increased by BAFLOP supplementation at both levels (T3 and T4; P < 0.05). Ruminal pH dropped (5.9) in buffaloes fed with concentrate at 15 g/kg BW, while buffaloes with BAFLOP supplementation could maintain ruminal pH when fed with high-concentrate diet. Ruminal ammonia-nitrogen increased in the buffaloes fed concentrate at 15 g/kg BW, especially with BAFLOP supplementation. Feeding high-concentrate diet increased the concentrations of ruminal total volatile fatty acids and propionic acid (C3), while the concentration of acetic acid and the acetic acid:C3 ratio and methane production were subsequently reduced (P < 0.05). In addition, efficiency of microbial protein synthesis was increased by the BAFLOP feeding (P < 0.05). In the present study, using BAFLOP as a dietary rumen enhancer at 300–600 g/head.day resulted in an increased rumen pH, C3 concentration, nutrient digestibility and microbial protein synthesis, while mitigating ruminal methane production. Higher nutrient digestibility and lower ruminal methane production, more dietary energy and production efficiency are expected.


2019 ◽  
Vol 52 (4) ◽  
pp. 1609-1615 ◽  
Author(s):  
Chaichana Suriyapha ◽  
Thiwakorn Ampapon ◽  
Bounnaxay Viennasay ◽  
Maharach Matra ◽  
Chinda Wann ◽  
...  

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1266
Author(s):  
Michelle Siqueira ◽  
Juana Chagas ◽  
João Paulo Monnerat ◽  
Carolina Monteiro ◽  
Robert Mora-Luna ◽  
...  

This study evaluated the effect of different roughages fed to sheep on nutrient and water intake, ingestive behavior, nitrogen balance, microbial protein synthesis, fermentation parameters, and methane production using an in vitro gas production system. The treatments consisted of five diets: cactus cladodes Nopalea (NUB) and Opuntia (OUB), both with the addition of sugarcane bagasse (SB) and urea/ammonium sulfate (urea/as); Tifton hay (TH); corn silage (CS); and sorghum silage (SS), also with added urea/as. The NUB provided greater (p ≤ 0.03) intakes of dry matter (1024 g/d), digestible organic matter (670 g/d), and crude protein (161 g/d) than those on the SS. The NUB provided greater (p < 0.01) dietary water intake (3023 g/d) than TH. The time spent on rumination was shorter (p < 0.01), and the idle time was longer in animals fed NUB and OUB than TH and CS. Microbial protein synthesis was not affected (p = 0.27). The final pH (6.4) of the incubation fluid and the concentration of NH3-N (39.05 mg/dL) were greater for NUB and OUB. Ruminal parameters and methane production were little or not affected by tested forages. We recommend using cactus cladodes in combination with sugarcane bagasse and urea/as in sheep diets.


Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 261 ◽  
Author(s):  
Chanadol Supapong ◽  
Anusorn Cherdthong ◽  
Metha Wanapat ◽  
Pin Chanjula ◽  
Sutipong Uriyapongson

The influence of sulfur included in fermented total mixed ration (FTMR) containing fresh cassava root on rumen characteristics, microbial protein synthesis, and blood metabolites in cattle was evaluated. Four Thai native beef cattle were randomly assigned according to a 2 × 2 factorial in a 4 × 4 Latin square design, and dietary treatments were as follows: factor A included a level of sulfur at 1% and 2% in total mixed ration, and factor B featured ensiling times at zero and 7 days. Digestibility of dry matter was increased when FTMR was supplemented with 2% sulfur. Blood thiocyanate increased by 69.5% when ensiling time was 7 days compared to no ensiling (p < 0.01). Bacterial populations were significantly different in the FTMR containing sulfur at 2% and 7 days of ensiling. Furthermore, microbial crude protein and efficiency of microbial protein synthesis were higher in the FTMR containing 2% sulfur and 7 days of ensiling (p < 0.01). Thus, high levels of hydrocyanic acid from fresh cassava root could be detoxified by a sulfur addition with an ensiling process to become nontoxic to cattle.


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