Improved glucose tolerance with restored expression of glucose transporter 4 in C57BL/6 mice after a long period of high-fat diet feeding

2014 ◽  
Vol 18 (3) ◽  
pp. 197-203 ◽  
Author(s):  
Jinil Kim ◽  
Ja In Jeong ◽  
Kwang Min Kim ◽  
Inho Choi ◽  
Richard E. Pratley ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
High Fat Diet ◽  
Glucose Transporter ◽  
Glucose Transporter 4 ◽  
High Fat ◽  
Long Period

scholarly journals Effects of Lactobacillus on Mice with Diabetes Induced by High-Fat Diet with Streptozotocin (STZ)

2018 ◽  
Vol 8 (8) ◽  
pp. 1249 ◽  
Author(s):  
Xiaoyong Chen ◽  
Fang Tan ◽  
Ruokun Yi ◽  
Jianfei Mu ◽  
Xin Zhao ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
High Fat Diet ◽  
Glucose Transporter ◽  
Regulatory Element ◽  
Hepatic Insulin Resistance ◽  
Average Weight ◽  
Oral Glucose ◽  
High Fat ◽  
Glut 4 ◽  
Tnf Α

This study aimed to evaluate and compare the effects of heat-killed and live Lactobacillus on mice with diabetes induced by high-fat diet with streptozotocin (STZ). Results based on body weight and liver pathological changes, oral glucose tolerance test, and related serum index (AST (aspartate aminotransferase), ALT (alanine aminotransferase), MDA (malondialdehyde), TNF-α (tumor necrosis factor α), INS (insulin), and GC (glucagon) and gene expression of IL-1β (Interleukin 1β), IRS-1(Insulin receptor substrate 1), GLUT-4 (glucose transporter type 4), PPARγ (peroxisome proliferators-activated receptor γ), and SREBP-1c (sterol-regulatory element-binding protein-1c) levels indicated that Lactobacillus fermentum (LF) and Lactobacillus plantarum (LP) could increase the average weight, alleviate the degree of damage in the liver, and improve the glucose tolerance of mice with diabetes. LF and LP also participated in the downregulation of AST, ALT, MDA, TNF-α, INS, and GC in serum, as well as the inhibition of IL-1β, TNF-α, IRS-1, GLUT-4, PPARγ, and SREBP-1c expression. These regulating effects were remarkable, and the regulating effect of the live group was significantly better than that of the heat-killed group. This study suggested that LF and LP can significantly alleviate liver damage and hepatic insulin resistance in mice with diabetes and that the acting mechanisms of LF and LP were related to cellular components and their activities.

Comparison between pre-exercise casein peptide and intact casein supplementation on glucose tolerance in mice fed a high-fat diet

2018 ◽  
Vol 43 (4) ◽  
pp. 355-362 ◽  
Author(s):  
Yutaka Matsunaga ◽  
Yuki Tamura ◽  
Yasuyuki Sakata ◽  
Yudai Nonaka ◽  
Noriko Saito ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Endurance Training ◽  
Soleus Muscle ◽  
High Fat Diet ◽  
Glucose Transporter ◽  
Area Under The Curve ◽  
Abdominal Fat ◽  
Oral Glucose ◽  
High Fat

We hypothesized that along with exercise, casein peptide supplementation would have a higher impact on improving glucose tolerance than intact casein. Male 6-week-old ICR mice were provided a high-fat diet to induce obesity and glucose intolerance. The mice were randomly divided into 4 treatment groups: control (Con), endurance training (Tr), endurance training with intact casein supplementation (Cas+Tr), and endurance training with casein peptide supplementation (CP+Tr). The mice in each group were orally administrated water, intact casein, or casein peptide (1.0 mg/g body weight, every day), and then subjected to endurance training (15–25 m/min, 60 min, 5 times/week for 4 weeks) on a motor-driven treadmill 30 min after ingestion. Our results revealed that total intra-abdominal fat was significantly lower in CP+Tr than in Con (p < 0.05). Following an oral glucose tolerance test, the blood glucose area under the curve (AUC) was found to be significantly smaller for CP+Tr than for Con (p < 0.05). Moreover, in the soleus muscle, glucose transporter 4 (GLUT4) protein levels were significantly higher in CP+Tr than in Con (p < 0.01). However, intra-abdominal fat, blood glucose AUC, and GLUT4 protein content in the soleus muscle did not alter in Tr and Cas+Tr when compared with Con. These observations suggest that pre-exercise casein peptide supplementation has a higher effect on improving glucose tolerance than intact casein does in mice fed a high-fat diet.

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