scholarly journals Polydeoxyribonucleotide administration promotes the expression of proliferating cell nuclear antigen during liver regeneration in rats

2015 ◽  
Vol 19 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Kwang Ho Yoon ◽  
Wan Jong Kim ◽  
Sang Kyeun Han ◽  
Kook-Il Han ◽  
Kung-Woo Nam ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Proliferating Cell

scholarly journals Effect of the aqueous extract of Sida cordifolia on liver regeneration after partial hepatectomy

2006 ◽  
Vol 21 (suppl 1) ◽  
pp. 37-39 ◽  
Author(s):  
Renata Lemos Silva ◽  
Gustavo Barreto de Melo ◽  
Valdinaldo Aragão de Melo ◽  
Ângelo Roberto Antoniolli ◽  
Paulo Roberto Teixeira Michellone ◽  
...  
Keyword(s):  
Oral Administration ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Aqueous Extract ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Hepatic Regeneration ◽  
Control Group ◽  
Sida Cordifolia ◽  
Proliferating Cell

PURPOSE: The use of medicinal plants for the treatment of human diseases has increased worldwide. Many of them are used by oral administration and, after absorption, may affect many organs. Therefore, this study aimed at assessing the effects of the aqueous extract of Sida cordifolia leaves, popularly known in Brazil as "malva-branca", on liver regeneration. METHODS: Twenty rats were divided into four groups: control, Sida100, Sida200 and Sida400 groups. All animals were submitted to oral administration of distilled water, 100, 200 and 400 mg/kg of the aqueous extract of Sida cordifolia, respectively. Immediately after this, they underwent 67% partial hepatectomy. Twenty four hours later, their livers were removed. Hepatic regeneration was assessed by immunohistochemical staining for proliferating cell nuclear antigen (PCNA) using the PC-10 monoclonal antibody. RESULTS: Sida100 and Sida200 groups disclosed higher liver regeneration indices than control group (p<0.001 and p<0.05, respectively). CONCLUSION: The aqueous extract of Sida cordifolia stimulates liver regeneration after 67% partial hepatectomy in rats.

scholarly journals Proliferative effect of the aqueous extract of Hyptis pectinata on liver regeneration after partial hepatectomy in rats

2006 ◽  
Vol 21 (suppl 1) ◽  
pp. 33-36 ◽  
Author(s):  
Gustavo Barreto Melo ◽  
Renata Lemos Silva ◽  
Valdinaldo Aragão Melo ◽  
Ângelo Roberto Antoniolli ◽  
Paulo Roberto Teixeira Michellone ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Aqueous Extract ◽  
Proliferating Cell Nuclear Antigen ◽  
Serum Enzymes ◽  
Nuclear Antigen ◽  
Proliferative Effect ◽  
Water Administration ◽  
Proliferating Cell ◽  
Hepatic Protection

PURPOSE: This study was carried out to assess the effects of the aqueous extract of Hyptis pectinata leaves on liver regeneration and on serum enzymes (AST, ALT and gamma-GT) after 67% partial hepatectomy in rats. METHODS: AST, ALT and gamma-GT, were determined by conventional procedures using a spectrophotometer (Model E2250-CELM). Liver regeneration was evaluated by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). RESULTS:Oral pretreatment during 4 days at 100 mg/kg increased liver regeneration index. At 200 mg/kg, AST level was statistically decreased in comparison to the group submited to distilled water administration. The other enzymes assessed disclosed no difference when all groups were compared. CONCLUSION: The present study shows that the aqueous extract of Hyptis pectinata leaves contains some biological active principles that stimulate liver regeneration at 100 mg/kg and cause slight hepatic protection at 200 mg/kg.

scholarly journals Biological Role of Proliferating Cell Nuclear Antigen (PCNA) Expression during Rat Liver Regeneration.

1994 ◽  
Vol 27 (2) ◽  
pp. 135-140 ◽  
Author(s):  
TOMOKI NAKAJIMA ◽  
KEIZO KAGAWA ◽  
TAKESHI DEGUCHI ◽  
HIROSHI HIKITA ◽  
TAKESHI OKANOUE ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Rat Liver ◽  
Proliferating Cell Nuclear Antigen ◽  
Biological Role ◽  
Nuclear Antigen ◽  
Rat Liver Regeneration ◽  
Proliferating Cell

scholarly journals Immunohistochemical Study of the Ameliorative Effect of Vitamin E on Liver Regeneration after Different Periods of Partial Hepatectomy

2018 ◽  
Vol 11 (2) ◽  
pp. 661-669
Author(s):  
Mervat M. Halawani ◽  
Gamal S. Abdul Aziz ◽  
Hanan A. Amin ◽  
Hesham N. Mustafa ◽  
Amira A. Elhaggagy
Keyword(s):  
Vitamin E ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Proliferating Cell Nuclear Antigen ◽  
Inhibitory Effect ◽  
Nuclear Antigen ◽  
Albino Rats ◽  
Ameliorative Effect ◽  
Proliferating Cell ◽  
Control Sham

The liver is almost unique in its capacity for regeneration after hepatectomy but the exact mechanisms are not yet fully clarified. Antioxidants have been shown to promote liver regeneration after major hepatectomy. The present study evaluated the ameliorative effect of vitamin E administration on the liver regeneration after different periods of partial hepatectomy (PH) in rats. Fifty-six adult male albino rats were divided into three groups: Control sham operated group; partially hepatectomized group which were divided into three subgroups sacrificed at 1day, 3 days and 7days after the operation respectively; Partially Hepatectomized group with vitamin E pretreatment before PH where the rats were given a daily oral dose of vitamin E until the time of sacrifice of the rats. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and labeling index were demonstrated. After PH, the PCNA positive hepatocytes and the PCNA labeling indices were significantly high after the 1st day and then much decreased after the 3rd day, to be followed by a slight increase at the 7th day. Vitamin E pretreatment in PH rats resulted in a decrease in PCNA positive cells and its labeling indices in the 1st day with a gradual increase in the 3rd and 7th days. Vitamin E has an inhibitory effect in the first 24 hours on liver regeneration followed by stimulatory effect at the third and seventh days after PH. These data indicated that vitamin E pretreatment has an important role in regulation and enhancement of liver regeneration after PH.

Bone marrow cell transplantation is associated with fibrogenic cells apoptosis during hepatic regeneration in cholestatic rats

2013 ◽  
Vol 91 (2) ◽  
pp. 88-94 ◽  
Author(s):  
Simone Nunes de Carvalho ◽  
Dalvaci da Cunha Lira ◽  
Erika Afonso Costa Cortez ◽  
Daniela Caldas de Andrade ◽  
Alessandra Alves Thole ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Liver Fibrosis ◽  
Confocal Microscopy ◽  
Liver Regeneration ◽  
Proliferating Cell Nuclear Antigen ◽  
Caspase 3 ◽  
Nuclear Antigen ◽  
Hepatic Regeneration ◽  
Extracellular Matrix Components ◽  
Proliferating Cell

Liver fibrosis is accompanied by hepatocyte death and proliferation of α-SMA+ fibrogenic cells (activated hepatic stellate cells and myofibroblasts), which synthesize extracellular matrix components that contribute to disorganization of the hepatic parenchyma and loss of liver function. Therefore, apoptosis of these fibrogenic cells is important to hepatic regeneration. This study aimed to analyze the effect of cell therapy using bone marrow mononuclear cell (BMMNC) transplantation on α-SMA expression and on apoptosis of hepatic cells during liver fibrosis induced by bile duct ligation (BDL). Livers were collected from normal rats, fibrotic rats after 14 and 21 days of BDL, and rats that received BMMNC at 14 days of BDL and were analyzed after 7 days. Apoptosis in fibrogenic cells was analyzed by immunoperoxidase, confocal microscopy, and Western blotting, and liver regeneration was assessed by proliferating cell nuclear antigen staining. Results showed that caspase-3 and proliferating cell nuclear antigen expression were significantly increased in the BMMNC-treated group. Additionally, confocal microscopy analysis showed cells coexpressing α-SMA and caspase-3 in these animals, suggesting fibrogenic cell death. These results suggest a novel role for BMMNC in liver regeneration during fibrotic disease by stimulating fibrogenic cells apoptosis and hepatocyte proliferation, probably through secretion of specific cytokines that modulate the hepatic microenvironment toward an antifibrogenic balance.

scholarly journals TRANEXAMIC ACID ACTION ON LIVER REGENERATION AFTER PARTIAL HEPATECTOMY: EXPERIMENTAL MODEL IN RATS

2016 ◽  
Vol 29 (2) ◽  
pp. 102-104 ◽  
Author(s):  
Felipe Antonio SOBRAL ◽  
Henrique DAGA ◽  
Henrique Nogueira RASERA ◽  
Matheus da Rocha PINHEIRO ◽  
Igor Furlan CELLA ◽  
...  
Keyword(s):  
Tranexamic Acid ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Regeneration Time ◽  
Organ Regeneration ◽  
Therapeutic Procedures ◽  
Proliferating Cell ◽  
Mitotic Cells

ABSTRACT Background: Different lesions may affect the liver resulting in harmful stimuli. Some therapeutic procedures to treat those injuries depend on liver regeneration to increase functional capacity of this organ. Aim: Evaluate the effects of tranexamic acid on liver regeneration after partial hepatectomy in rats. Method: 40 rats (Rattus norvegicus albinus, Rodentia mammalia) of Wistar-UP lineage were randomly divided into two groups named control (CT) and tranexamic acid (ATX), with 20 rats in each. Both groups were subdivided, according to liver regeneration time of 32 h or seven days after the rats had been operated. The organ regeneration was evaluated through weight and histology, stained with HE and PCNA. Results: The average animal weight of ATX and CT 7 days groups before surgery were 411.2 g and 432.7 g, and 371.3 g and 392.9 g after the regeneration time, respectively. The average number of mitotic cells stained with HE for the ATX and CT 7 days groups were 33.7 and 32.6 mitosis, and 14.5 and 14.9 for the ATX and CT 32 h groups, respectively. When stained with proliferating cell nuclear antigen, the numbers of mitotic cells counted were 849.7 for the ATX 7 days, 301.8 for the CT 7 days groups, 814.2 for the ATX 32 hand 848.1 for the CT 32 h groups. Conclusion: Tranexamic acid was effective in liver regeneration, but in longer period after partial hepatectomy.

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