scholarly journals Proliferative effect of the aqueous extract of Hyptis pectinata on liver regeneration after partial hepatectomy in rats

2006 ◽  
Vol 21 (suppl 1) ◽  
pp. 33-36 ◽  
Author(s):  
Gustavo Barreto Melo ◽  
Renata Lemos Silva ◽  
Valdinaldo Aragão Melo ◽  
Ângelo Roberto Antoniolli ◽  
Paulo Roberto Teixeira Michellone ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Aqueous Extract ◽  
Proliferating Cell Nuclear Antigen ◽  
Serum Enzymes ◽  
Nuclear Antigen ◽  
Proliferative Effect ◽  
Water Administration ◽  
Proliferating Cell ◽  
Hepatic Protection

PURPOSE: This study was carried out to assess the effects of the aqueous extract of Hyptis pectinata leaves on liver regeneration and on serum enzymes (AST, ALT and gamma-GT) after 67% partial hepatectomy in rats. METHODS: AST, ALT and gamma-GT, were determined by conventional procedures using a spectrophotometer (Model E2250-CELM). Liver regeneration was evaluated by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). RESULTS:Oral pretreatment during 4 days at 100 mg/kg increased liver regeneration index. At 200 mg/kg, AST level was statistically decreased in comparison to the group submited to distilled water administration. The other enzymes assessed disclosed no difference when all groups were compared. CONCLUSION: The present study shows that the aqueous extract of Hyptis pectinata leaves contains some biological active principles that stimulate liver regeneration at 100 mg/kg and cause slight hepatic protection at 200 mg/kg.

scholarly journals Effect of the aqueous extract of Sida cordifolia on liver regeneration after partial hepatectomy

2006 ◽  
Vol 21 (suppl 1) ◽  
pp. 37-39 ◽  
Author(s):  
Renata Lemos Silva ◽  
Gustavo Barreto de Melo ◽  
Valdinaldo Aragão de Melo ◽  
Ângelo Roberto Antoniolli ◽  
Paulo Roberto Teixeira Michellone ◽  
...  
Keyword(s):  
Oral Administration ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Aqueous Extract ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Hepatic Regeneration ◽  
Control Group ◽  
Sida Cordifolia ◽  
Proliferating Cell

PURPOSE: The use of medicinal plants for the treatment of human diseases has increased worldwide. Many of them are used by oral administration and, after absorption, may affect many organs. Therefore, this study aimed at assessing the effects of the aqueous extract of Sida cordifolia leaves, popularly known in Brazil as "malva-branca", on liver regeneration. METHODS: Twenty rats were divided into four groups: control, Sida100, Sida200 and Sida400 groups. All animals were submitted to oral administration of distilled water, 100, 200 and 400 mg/kg of the aqueous extract of Sida cordifolia, respectively. Immediately after this, they underwent 67% partial hepatectomy. Twenty four hours later, their livers were removed. Hepatic regeneration was assessed by immunohistochemical staining for proliferating cell nuclear antigen (PCNA) using the PC-10 monoclonal antibody. RESULTS: Sida100 and Sida200 groups disclosed higher liver regeneration indices than control group (p<0.001 and p<0.05, respectively). CONCLUSION: The aqueous extract of Sida cordifolia stimulates liver regeneration after 67% partial hepatectomy in rats.

scholarly journals Immunohistochemical Study of the Ameliorative Effect of Vitamin E on Liver Regeneration after Different Periods of Partial Hepatectomy

2018 ◽  
Vol 11 (2) ◽  
pp. 661-669
Author(s):  
Mervat M. Halawani ◽  
Gamal S. Abdul Aziz ◽  
Hanan A. Amin ◽  
Hesham N. Mustafa ◽  
Amira A. Elhaggagy
Keyword(s):  
Vitamin E ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Proliferating Cell Nuclear Antigen ◽  
Inhibitory Effect ◽  
Nuclear Antigen ◽  
Albino Rats ◽  
Ameliorative Effect ◽  
Proliferating Cell ◽  
Control Sham

The liver is almost unique in its capacity for regeneration after hepatectomy but the exact mechanisms are not yet fully clarified. Antioxidants have been shown to promote liver regeneration after major hepatectomy. The present study evaluated the ameliorative effect of vitamin E administration on the liver regeneration after different periods of partial hepatectomy (PH) in rats. Fifty-six adult male albino rats were divided into three groups: Control sham operated group; partially hepatectomized group which were divided into three subgroups sacrificed at 1day, 3 days and 7days after the operation respectively; Partially Hepatectomized group with vitamin E pretreatment before PH where the rats were given a daily oral dose of vitamin E until the time of sacrifice of the rats. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and labeling index were demonstrated. After PH, the PCNA positive hepatocytes and the PCNA labeling indices were significantly high after the 1st day and then much decreased after the 3rd day, to be followed by a slight increase at the 7th day. Vitamin E pretreatment in PH rats resulted in a decrease in PCNA positive cells and its labeling indices in the 1st day with a gradual increase in the 3rd and 7th days. Vitamin E has an inhibitory effect in the first 24 hours on liver regeneration followed by stimulatory effect at the third and seventh days after PH. These data indicated that vitamin E pretreatment has an important role in regulation and enhancement of liver regeneration after PH.

scholarly journals TRANEXAMIC ACID ACTION ON LIVER REGENERATION AFTER PARTIAL HEPATECTOMY: EXPERIMENTAL MODEL IN RATS

2016 ◽  
Vol 29 (2) ◽  
pp. 102-104 ◽  
Author(s):  
Felipe Antonio SOBRAL ◽  
Henrique DAGA ◽  
Henrique Nogueira RASERA ◽  
Matheus da Rocha PINHEIRO ◽  
Igor Furlan CELLA ◽  
...  
Keyword(s):  
Tranexamic Acid ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Regeneration Time ◽  
Organ Regeneration ◽  
Therapeutic Procedures ◽  
Proliferating Cell ◽  
Mitotic Cells

ABSTRACT Background: Different lesions may affect the liver resulting in harmful stimuli. Some therapeutic procedures to treat those injuries depend on liver regeneration to increase functional capacity of this organ. Aim: Evaluate the effects of tranexamic acid on liver regeneration after partial hepatectomy in rats. Method: 40 rats (Rattus norvegicus albinus, Rodentia mammalia) of Wistar-UP lineage were randomly divided into two groups named control (CT) and tranexamic acid (ATX), with 20 rats in each. Both groups were subdivided, according to liver regeneration time of 32 h or seven days after the rats had been operated. The organ regeneration was evaluated through weight and histology, stained with HE and PCNA. Results: The average animal weight of ATX and CT 7 days groups before surgery were 411.2 g and 432.7 g, and 371.3 g and 392.9 g after the regeneration time, respectively. The average number of mitotic cells stained with HE for the ATX and CT 7 days groups were 33.7 and 32.6 mitosis, and 14.5 and 14.9 for the ATX and CT 32 h groups, respectively. When stained with proliferating cell nuclear antigen, the numbers of mitotic cells counted were 849.7 for the ATX 7 days, 301.8 for the CT 7 days groups, 814.2 for the ATX 32 hand 848.1 for the CT 32 h groups. Conclusion: Tranexamic acid was effective in liver regeneration, but in longer period after partial hepatectomy.

Effect of natural antioxidants and apocynin on LPS-induced endotoxemia in rabbit

2000 ◽  
Vol 19 (11) ◽  
pp. 604-614 ◽  
Author(s):  
V Ben-Shaul ◽  
L Lomnitskil ◽  
A Nyska ◽  
M Carbonatto ◽  
S Peano ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Proliferating Cell Nuclear Antigen ◽  
Immunohistochemical Staining ◽  
Microsomal Fraction ◽  
Natural Antioxidants ◽  
Treated Group ◽  
Nuclear Antigen ◽  
Sod Activity ◽  
Proliferative Effect ◽  
Proliferating Cell

The objective of this study was to compare the prophylactic effects of the natural antioxidant from spinach (NAO) and apocynin, on the hepatic oxidative stress and liver damage induced by lipopolysaccharide (LPS). Male New Zealand rabbits were challenged with LPS with or without 8 days of antioxidant pretreatment. Pretreatment with NAO, but not apocynin, significantly (p<0.05) decreased the levels of hydroperoxides and malondialdehyde (MDA) in the liver cytosolic fraction and the activity of NADPH oxidase-generated superoxide in the microsomal fraction, compared to LPS alone. The activity of glutathione peroxidase (G-POX) was significantly (p<0.05) increased in the LPS-treated group, whereas treatment with NAO, but not apocynin, significantly (p< 0.05) decreased G-POX activity. Pretreatment with the same antioxidants had no significant effects on superoxide dismutase (SOD) activity, whereas an increased level of catalase (CAT) was obtained in all LPS-treated groups. TUNEL immunohistochemical staining in the LPS-treated animals indicated that there was no increase in apoptosis outside of necrotic foci. However, apoptotic hepatocytes were observed within areas of focal necrosis in animals exposed to LPS alone or LPS plus apocynin. Hepatocyte cell proliferation was tested by the proliferating-cell nuclear antigen (PCNA) tool, which indicated a proliferative effect in the LPS group, whereas the effect disappeared in the antioxidant-treated groups. The prophylactic effect of NAO on liver pathology and the significant decreases in lipid peroxidation products and NADPH oxidase activity suggest the use of NAO as an efficient strategy for treatanent of endotoxemia.

scholarly journals Proliferative effect of aqueous extract of Hyptis fructicosa on liver regeneration after partial hepatectomy in rats

2012 ◽  
Vol 27 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Sônia Oliveira Lima ◽  
Luciano da Costa Viana ◽  
Fábio Rafael Teixeira de Santana ◽  
Ségio Zucoloto ◽  
Ricardo Luiz de Albuquerque Junior ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Aqueous Extract ◽  
Hepatocyte Proliferation ◽  
Hepatic Regeneration ◽  
Control Group ◽  
Immunohistochemical Technique ◽  
Proliferative Effect

PURPOSE: To evaluate the effect of aqueous extract of Hyptis fructicosa on hepatic regeneration after partial hepatectomy in rats. METHODS: Sixteen rats were divided in two groups: C (Control Group) and HF (Whose rats received aqueous extract of Hyptis fructicosa during 4 days using the dose of 100 mg/kg/day). On the consecutive day of this treatment, the animals of both groups underwent hepatectomy of about 67% of liver. Twenty four hours later, they were sacrificed, and the remaining mass of liver was removed and prepared to be studied through the PCNA immunohistochemical technique. RESULTS: The liver regeneration index of HF group was 53.56 ± 18.91%, while in C group was 21.12 ± 8.29% (p=0.0003). CONCLUSION: These results show that the administration of aqueous extract of Hyptis fructicosa using the dose of 100mg/kg/day increased the hepatocyte proliferation in the group HF.

scholarly journals Resina Draconis Reduces Acute Liver Injury and Promotes Liver Regeneration after 2/3 Partial Hepatectomy in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Zhi-yong He ◽  
Kai-han Lou ◽  
Jia-hui Zhao ◽  
Ming Zhang ◽  
Lan-chun Zhang ◽  
...  
Keyword(s):  
Liver Injury ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Acute Liver Injury ◽  
Liver Weight ◽  
Nuclear Antigen ◽  
Protective Effects ◽  
Weight Restoration ◽  
Resina Draconis ◽  
Proliferating Cell

Aim. To investigate the protective effects and possible mechanisms of action of resina draconis (RD) on acute liver injury and liver regeneration after 2/3 partial hepatectomy (PH) in mice. Methods. 2/3 PH was used to induce acute liver injury. Mice were divided into three groups: sham, vehicle + 2/3 PH, and RD + 2/3 PH. Resina draconis was administered intragastrically after 2/3 PH into the RD + 2/3 PH group, and the same volume of vehicle (1% sodium carboxymethyl cellulose) was injected into the vehicle + 2/3 PH group and sham group mice. The index of liver to body weight (ILBW) and proliferating cell nuclear antigen (PCNA) were assayed to evaluate liver regeneration. Blood and liver tissues were collected for serological and western blotting analysis. Results. Resina draconis protected against 2/3 PH-induced acute severe liver injury and promoted liver regeneration as shown by significantly increased ILBW compared with that of controls. 2/3 PH increased serum AST and ALT levels, which were significantly decreased by RD treatment, while 2/3 PH decreased serum TP and ALB, which were increased by RD treatment. In the RD + 2/3 PH group, PCNA expression was significantly increased compared with the 2/3 PH group. Further, hepatocyte growth factor (HGF), TNFα, and EGFR levels were increased in the RD group at postoperative days 2 and 4 compared with the those in the 2/3 PH group. Conclusion. Our results suggest that RD ameliorates acute hepatic injury and promotes liver cell proliferation, liver weight restoration, and liver function after 2/3 PH, probably via HGF, TNFα, and EGFR signaling.

scholarly journals The effect of laser on remanescent liver tissue after 90% hepatectomy in rats

2006 ◽  
Vol 21 (suppl 1) ◽  
pp. 29-32 ◽  
Author(s):  
Alexandre Ferreira Oliveira ◽  
Tiago Castro e Silva ◽  
Ajith Kumar Sankarankutty ◽  
Eduardo Garcia Pacheco ◽  
Juliana Ferreira ◽  
...  
Keyword(s):  
Partial Hepatectomy ◽  
Proliferating Cell Nuclear Antigen ◽  
Proliferative Activity ◽  
Wistar Rats ◽  
Liver Parenchyma ◽  
Serum Levels ◽  
Nuclear Antigen ◽  
Serum Aminotransferases ◽  
Hepatic Injuries ◽  
Proliferating Cell

PURPOSE: To evaluate the effect of laser beam on remanescent liver after partial hepatectomy 90%. METHODS: Wistar rats, (N= 42), were divided into six groups with seven specimens each. The partial hepatectomy (HP) was performed in all animals through exeresis of approximately 90% of the liver parenchyma. The animals from groups HP and Laser application, HPL24, HPL48 and HPL72 undertook laser irradiation carried out through application (dose of 22.5 J/cm²) in five different sites in the remanescent liver. The rats were then sacrificed 24, 48 and 72hours after HP procedure, for the liver regeneration analysis,using the Proliferating Cell Nuclear Antigen (PCNA),and for dosage of serum aminotransferases. RESULTS: Were demonstrated an increase of the serum levels of alanine aminotransferase for the group of 24 hours and a decrease for the group of 72 hours exposed to laser. The index of marked cells had a considerable more improvement for the group of 72 hours exposed in laser compared to other groups. CONCLUSION: Laser did not cause hepatic injuries additional to the partial hepatectomy and perhaps led to a benefit by stimulating the proliferative activity.

Proliferating cell nuclear antigen (PCNA) expression in regenerating rat liver after partial hepatectomy

1994 ◽  
Vol 39 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Stamatios E. Theocharis ◽  
Antigone S. Skopelitou ◽  
Alexandra P. Margeli ◽  
Kitty J. Pavlaki ◽  
Christos Kittas
Keyword(s):  
Partial Hepatectomy ◽  
Rat Liver ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Regenerating Rat Liver ◽  
Proliferating Cell
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