scholarly journals Effect of extraction solvent on qualitative and quantitative analysis of major phyto-constituents and in-vitro antioxidant activity evaluation of Cadaba rotundifolia Forssk leaf extracts

2020 ◽  
Vol 6 (1) ◽  
pp. 1853867
Author(s):  
Teshome Gonfa ◽  
Shewangizawe Teketle ◽  
Tsegu Kiros
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Qualitative And Quantitative Analysis ◽  
Leaf Extracts ◽  
Qualitative And Quantitative ◽  
Extraction Solvent ◽  
Activity Evaluation ◽  
In Vitro Antioxidant Activity

scholarly journals A Review on Chromatography—Mass Spectrometry Applications on Anthocyanin and Ellagitannin Metabolites of Blackberries and Raspberries

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2150
Author(s):  
Dilip K. Rai ◽  
Katerina Tzima
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Health Effects ◽  
Analytical Techniques ◽  
In Vitro Models ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Chromatography Mass Spectrometry

Berries have been widely assessed for their beneficial health effects, predominately due to their high (poly)phenol content of anthocyanins and ellagitannins. After ellagitannins and ellagic acid are metabolized by the gut microbiome, a class of compounds known as urolithins are produced, which exert potential advantageous health effects. Anthocyanins, on the other hand, undergo a complex metabolic pathway after their interaction with microbial and endogenous enzymes, forming a broad range of metabolites and catabolic products. In most cases, in vitro models and cell lines are used to generate metabolites, whereas their assessment in vivo is currently limited. Thus far, several analytical methods have been developed for the qualitative and quantitative analysis of phenolic metabolites in berries, including liquid chromatography, mass spectrometry, and other hyphenated techniques, and have been undoubtedly valuable tools for the detailed metabolite characterization and profiling. In this review, a compilation of studies providing information on the qualitative and quantitative analysis of (poly)phenol metabolites in blackberries and raspberries after the utilization of in vitro and in vivo methods is presented. The different analytical techniques employed are assessed, focusing on the fate of the produced metabolic compounds in order to provide evidence on their characteristics, formation, and beneficial effects.

scholarly journals Comparative Study of Metabolomic Profile and Antioxidant Content of Adult and In Vitro Leaves of Aristotelia chilensis

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 37
Author(s):  
Karina Andrea Crisóstomo-Ayala ◽  
Ana Belén Sabater-Jara ◽  
Claudia Pérez Manriquez ◽  
Federico Ferreres ◽  
Ángel Gil-Izquierdo ◽  
...  
Keyword(s):  
Total Phenolic ◽  
Qualitative And Quantitative Analysis ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Lipophilic Compounds ◽  
Qualitative And Quantitative ◽  
Pharmaceutical Industries ◽  
Aristotelia Chilensis ◽  
Excellent Source

This work aimed to identify the bioactive compounds present in adult maqui (Aristotelia chilensis) leaves from different stages of development and seasons of the year and compare them with leaves obtained from maqui plants grown in vitro. The qualitative and quantitative analysis of maqui leaf extracts by HPLC-DAD-ESI-MSn showed the presence of different polyphenolic compounds classified into galloyl and caffeoyl quinic acids, ellagitannins and ellagic acid- and flavonoid-derivatives. In general, the total phenolic content of the in vitro samples was higher than that of ex vitro samples, whereas the total flavonoid content was higher in winter basal leaves. Additionally, the analysis by HPLC-MS showed that the extract from spring basal leaves was enriched in quercetin, catechin, kaempferol and 3-caffeoyl quinic acids, while in the in vitro leaves extract, quercetin was not present. As regards lipophilic compounds identified by GC/MS, the samples of in vitro leaves showed a high presence of α-tocopherol and β-sitosterol. In contrast, the samples of adult leaves presented a hight level of linolenic and linoleic acids. These results suggest that maqui leaves could be an excellent source of antioxidants and lipophilic compounds for many industries, such as the nutraceutical and pharmaceutical industries.

scholarly journals Isolation of Arbutin from Leaves and Fruits of Buni (Antidesma Bunius L. Spreng) As Tyrosinase Enzym Inhibitor

2021 ◽  
Vol 4 (1) ◽  
pp. 8-18
Author(s):  
Gea Ocktiah Palensina ◽  
Leni Rosita ◽  
Zuraida Sagala
Keyword(s):  
Quantitative Analysis ◽  
Mobile Phase ◽  
Enzyme Inhibitor ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Extractive Substances ◽  
Ic50 Values ◽  
Rf Values ◽  
Tyrosinase Enzyme

Several studies have shown that plant extractive substances have the potential as active compounds inhibiting the enzyme tyrosinase. Arbutin is an enzyme inhibitor of tyrosinase, known as a popular whitening agent used in cosmetics because of its effectiveness in overcoming skin hyperpigmentation. The purpose of this study was to conduct a qualitative and quantitative analysis of arbutin on Buni Leaves and Fruits (Antidesma bunius L. Spreng). The raw simplicia used are mature and young buni leaves, green, red and purple buni fruits. The extraction method is maceration using methanol as solvent. The initial screening for arbutin content was carried out using thin layer chromatography (TLC) and dichlormethan:methanol 50:50 used as mobile phase. Isolation of arbutin content was carried out using Preparative TLC with the same eluent. Qualitative and quantitative analysis were performed using High Pressured Liquid Chromatography (HPLC) with mobile phase of acetonitrile: water 60:40. The tyrosinase enzyme inhibition activity test was then carried out in vitro using 96-well microplate, l-tyrosine and l-dopa were used as substrate at a wavelength of 492 nm. The Rf values obtained ??for mature buni leaves and green buni fruits, respectively 0.61 and 0,62. The retention time of HPLC chromatogram respectively 2,784 minutes and 2,758 minutes. Arbutin levels in leaves and fruits are 7.9 mg / g and 2 mg / g. The activity of the enzyme tyrosinase of mature buni leaves on L-dopa and L-tyrosine substrate were respectively stated as IC50 values ??of 88.7191ppm and 101.33347 ppm. The activity of the enzyme tyrosinase of the green buni fruit on L-dopa and L-tyrosine substrate respectively stated IC50 values ??of 198,0293 ppm and 246,1296 ppm.

scholarly journals Invitro Efficacy of antioxidant activity in ethanolic and aqueous leaf extracts of Andrographis paniculata Nees and Rhinacanthus nasutus Kurz

2020 ◽  
Vol 11 (4) ◽  
pp. 6301-6306
Author(s):  
Akilandeswari G ◽  
Bupesh G ◽  
Vijaya Anand A ◽  
Saradhadevi K M ◽  
Mayur Mausoom Phukan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Radical Scavenging ◽  
The Body ◽  
Andrographis Paniculata ◽  
Peroxide Radical ◽  
Leaf Extracts ◽  
Rhinacanthus Nasutus ◽  
In Vitro Antioxidant Activity

Plants are adundantly and are very promisive to be used as source of drugs in many diseases or infections and also it is a main agents of antioxidants which prevents the oxidative stress that are caused by the free radicals. There are numerous studies based on the pharmaceutical and classification of medicinal plants throughout the world. Leaves, fruits, roots are most frequently plant parts used in many research and studies. Here the present study was aimed to evaluate the in vitro antioxidant activity of aqueous and ethanol leaf extracts of Andrographis paniculata Nees and Rhinacanthus nasutus Kurz. Antioxidant is a substance which is used to prevent some types of cell damage in the body. Determination of their in vitro antioxidant activity were carried out by using methods such as DPPH (2,2-diphenyl-1picrylhydrazyl assay), ABTS (2,2′-azinobis (3-ethylbenzothiazolin 6-sulfonic acid assay), FRAP (ferric reducing antioxidant power assay) and SOD (super oxide anion scavenging) assay, H2O2 (hydrogen peroxide radical scavenging) assay. Moreover the ethanolic leaf extracts showed best antioxidant activity than the aqueous leaf extracts. Experimental results reveals that the leaves of A. paniculata have potent antioxidant and free radical scavenging activity than R. nasutus. Further investigation must be done for these two medicinal plants for the discovery of the bioactive compounds.

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