Mitochondrial heteroplasmy and pseudogenes in the freshwater prawn, Macrobrachium amazonicum (Heller, 1862): DNA barcoding and phylogeographic implications

The migratory behavior of females of the freshwater prawn Macrobrachium amazonicum (Heller, 1862) can indicate a strategy that optimizes the population establishment. With this idea in mind, we evaluated the reproductive biology of M. amazonicum, hypothesizing that females were evenly distributed downstream of Hydroelectric Dam. Specimens were collected monthly for one year, from six sites of Rio Grande river. The specimens were sexed and measured (carapace length; CL). A total of 14,697 adults were captured, 2,864 males (AM), 11,082 non-breeding females (AF) and 751 breeding females (BF). The smallest BF had 3.8 mm CL. The distribution of demographic groups was assessed by a Principal Component Analysis (PCA), which explained 95.16% of the distribution over the collection sites. Breeding females were more abundant in the sites closest to the dam. This result can be explained by rainfall, which varied significantly throughout the year. As the breeding females migrated upstream toward the dam and were more abundant there, our hypothesis of homogeneous distribution was rejected. This behavior probably optimizes larval dispersion. The reproduction was continuous with peaks in the period preceding the maximum rainfall.

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The stage of ovarian development affects organ expression of vitellogenin as well as the morphometry and ultrastructure of germ cells in the freshwater prawn Macrobrachium amazonicum (Heller, 1862)

Theriogenology ◽

10.1016/j.theriogenology.2012.04.019 ◽

2012 ◽

Vol 78 (5) ◽

pp. 981-990 ◽

Cited By ~ 5

Author(s):

M.A.P. Ferreira ◽

B.M. Resende ◽

M.Y.S. Lima ◽

S.S.D. Santos ◽

R.M. Rocha

Keyword(s):

Germ Cells ◽

Ovarian Development ◽

Freshwater Prawn ◽

Macrobrachium Amazonicum

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Myology of juvenile freshwater prawn Macrobrachium amazonicum (Decapoda, Caridea): Morphology and swimming implication

Arthropod Structure & Development ◽

10.1016/j.asd.2020.100973 ◽

2020 ◽

Vol 58 ◽

pp. 100973

Author(s):

Thalles Fernando Rocha Ruiz ◽

Gabriel Vieira Martins Rezende Gois ◽

João Candido Rodrigues Rocha ◽

Mateus Rossetto Vidal ◽

Mario Vitor Buzete Gardinal ◽

...

Keyword(s):

Freshwater Prawn ◽

Macrobrachium Amazonicum

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Toxicity of cryoprotectants agents in freshwater prawn embryos of Macrobrachium amazonicum

Zygote ◽

10.1017/s0967199414000458 ◽

2014 ◽

Vol 23 (6) ◽

pp. 813-820 ◽

Cited By ~ 5

Author(s):

Arthur Vinícius Lourenço Ferreira ◽

Elias José Teles Castro ◽

Mariana Silva Alves Barbosa ◽

Míriam Luzia Nogueira Martins de Sousa ◽

Manoel Paiva de Araújo Neto ◽

...

Keyword(s):

Ethylene Glycol ◽

Dimethyl Sulfoxide ◽

Present Experiment ◽

Morphological Analysis ◽

Freshwater Prawn ◽

Toxicity Tests ◽

Significance Level ◽

Random Samples ◽

Macrobrachium Amazonicum

SummaryThe process of cooling and cryopreservation of prawn embryos is a viable alternative for a continuous supply of larvae for freshwater prawn farming ponds. However, studies involving the application of those techniques as well as on toxicity of cryoprotectants in freshwater prawn embryos are scarce. Thus, this study aims to test the toxicity of methylic alcohol (MET), dimethyl sulfoxide (DMSO) and ethylene glycol (EG) on Macrobrachium amazonicum embryos. For the present experiment, pools of embryos were taken from 15 M. amazonicum females and were divided into three groups and tested in duplicate at concentrations of 10, 5, 3; 1, 0.5 or 0.1%. Toxicity tests were conducted for 24 h in Falcon® pipes to obtain the lethal concentration for 50% of the larvae (LC50). After the set period for testing, random samples of embryos were removed for morphological analysis under stereoscopic microscopes. Results were analysed using analysis of variance (ANOVA) and Tukey's test at a 5% significance level and Trimmed Spearman-Karber Analysis to determine LC50-24 h. DMSO toxicity tests revealed that 5% and 10% concentrations showed the highest toxicity and differed from the control (P ≤ 0.05), 24h-LC50 was 437.4 ± 14.4 µL. MET was less toxic among the tested cryoprotectants and concentrations did not allow the determination of its LC50-24h. For tests with EG, concentrations of 3, 5 or 10% solutions resulted in a 100% mortality to tested embryos; EG was the tested cryoprotectant with the highest toxicity, with an LC50-24h average of 81.91 ± 35.3 µl.

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Reproductive cycle of Macrobrachium amazonicum females (Crustacea, Palaemonidae)

Brazilian Journal of Biology ◽

10.1590/s1519-69842007000300022 ◽

2007 ◽

Vol 67 (3) ◽

pp. 551-559 ◽

Cited By ~ 29

Author(s):

CMS. Sampaio ◽

RR. Silva ◽

JA. Santos ◽

SP. Sales

Keyword(s):

Environmental Parameters ◽

Sexual Cycle ◽

Freshwater Prawn ◽

Total Length ◽

In Captivity ◽

Maturation Stages ◽

Macrobrachium Amazonicum ◽

Monthly Sample ◽

Number Of Individuals ◽

Maturation Age

Macrobrachium amazonicum is considered a favorite Brazilian species of freshwater prawn for cultivation as a result of its quick development and because it is easy to maintain in captivity. The aim of this work is to describe the sexual cycle stages and determine maturation age of the female M. amazonicum, which was collected monthly from June, 2002 to May, 2003 in the Jaguaribe River, Itaiçaba, Ceará. A monthly sample of water was also collected to determine the following parameters: temperature, dissolved oxygen, pH and salinity. A monthly sample of females was selected among the individuals caught, to determine the total weight (W T), carapace length (L C) and abdomen+telson length (L A+T) and to register the number of non-ovigerous females (NOF) and ovigerous females (OF). Determining ovarian maturation stages of M. amazonicum was done in a laboratory by observing macroscopic characters such as coloring, size, location and appearance of ovarians examined by transparent carapace. The first maturation age was determined from the relative frequency of the total length (L T) of young and adult females. The environmental parameters of the Jaguaribe River did not hold any influence in the number of individuals collected. A total of 1,337 prawns were sampled, 513 males (38.4%) and 824 females (61.6%). The proportion between males and females in the studied population was of 1:1.6. Among the collected females, 492 (50.7%) did not carry eggs in their abdomens (NOF) and 332 (40.3%) carried eggs in their abdomens (OF). There was no record of intact females. Non-ovigerous females with mature ovaries were recorded throughout all the months of collection. The female ovaries were classified as immature (IM), rudimentary (RU), intermediary (IN) and mature (M). M. amazonicum females reach their first sexual maturity between 4.5 and 5.5 cm of total length.

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Histomorphologic characterization of the hepatopancreas of freshwater prawn Macrobrachium rosenbergii (De Man, 1879)

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-10497 ◽

2018 ◽

Vol 70 (5) ◽

pp. 1539-1546 ◽

Cited By ~ 1

Author(s):

M.A.S. Silva ◽

M.E. Almeida Neto ◽

B.O. Ramiro ◽

I.T.F. Santos ◽

R.R. Guerra

Keyword(s):

Macrobrachium Rosenbergii ◽

Distal Region ◽

Proximal Region ◽

Freshwater Prawn ◽

Cell Type ◽

Nutrient Storage ◽

Macrobrachium Amazonicum ◽

De Man ◽

First Time

ABSTRACT This study aimed to describe the Macrobrachium rosenbergii hepatopancreas histomorphology. The hepatopancreas is constituted by a set of blind end tubules, divided into proximal, middle, and distal regions, with the epithelium formed by E, F, B, R, and M cells differently of other species. Measurements of the length and width of the tubules were 419.64+69.09µm and 117.42+16.99µm, respectively. The percentage of each cell type per region was: proximal region (40%B, 20%F, 6.7%M, 33.3%R), middle region (45.4%B, 18.2%F, 9.1%M, and 27.3%R) and distal region (36.4%E, 27.2%B, 18.2%F, 9.1%M, 9.1%R). Cell B that stores glycogen and lipids, is the most commonly found cell in proximal and middle regions. In the distal region, the E, responsible for the mitosis, is the most prominent. M, responsible by nutrient storage, is numerically constant among the portions differently in the Macrobrachium amazonicum. The study for the first time also suggests that in addition to digestive enzymes, the F cell produces protective mucus. The present study generated for the first time a morphometric profile of M. rosenbergii hepatopancreas, demonstrating differences from other species, and can be an important tool for new studies in nutrition, reproduction, and production with the species.

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