Myology of juvenile freshwater prawn Macrobrachium amazonicum (Decapoda, Caridea): Morphology and swimming implication

The migratory behavior of females of the freshwater prawn Macrobrachium amazonicum (Heller, 1862) can indicate a strategy that optimizes the population establishment. With this idea in mind, we evaluated the reproductive biology of M. amazonicum, hypothesizing that females were evenly distributed downstream of Hydroelectric Dam. Specimens were collected monthly for one year, from six sites of Rio Grande river. The specimens were sexed and measured (carapace length; CL). A total of 14,697 adults were captured, 2,864 males (AM), 11,082 non-breeding females (AF) and 751 breeding females (BF). The smallest BF had 3.8 mm CL. The distribution of demographic groups was assessed by a Principal Component Analysis (PCA), which explained 95.16% of the distribution over the collection sites. Breeding females were more abundant in the sites closest to the dam. This result can be explained by rainfall, which varied significantly throughout the year. As the breeding females migrated upstream toward the dam and were more abundant there, our hypothesis of homogeneous distribution was rejected. This behavior probably optimizes larval dispersion. The reproduction was continuous with peaks in the period preceding the maximum rainfall.

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The stage of ovarian development affects organ expression of vitellogenin as well as the morphometry and ultrastructure of germ cells in the freshwater prawn Macrobrachium amazonicum (Heller, 1862)

Theriogenology ◽

10.1016/j.theriogenology.2012.04.019 ◽

2012 ◽

Vol 78 (5) ◽

pp. 981-990 ◽

Cited By ~ 5

Author(s):

M.A.P. Ferreira ◽

B.M. Resende ◽

M.Y.S. Lima ◽

S.S.D. Santos ◽

R.M. Rocha

Keyword(s):

Germ Cells ◽

Ovarian Development ◽

Freshwater Prawn ◽

Macrobrachium Amazonicum

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Toxicity of cryoprotectants agents in freshwater prawn embryos of Macrobrachium amazonicum

Zygote ◽

10.1017/s0967199414000458 ◽

2014 ◽

Vol 23 (6) ◽

pp. 813-820 ◽

Cited By ~ 5

Author(s):

Arthur Vinícius Lourenço Ferreira ◽

Elias José Teles Castro ◽

Mariana Silva Alves Barbosa ◽

Míriam Luzia Nogueira Martins de Sousa ◽

Manoel Paiva de Araújo Neto ◽

...

Keyword(s):

Ethylene Glycol ◽

Dimethyl Sulfoxide ◽

Present Experiment ◽

Morphological Analysis ◽

Freshwater Prawn ◽

Toxicity Tests ◽

Significance Level ◽

Random Samples ◽

Macrobrachium Amazonicum

SummaryThe process of cooling and cryopreservation of prawn embryos is a viable alternative for a continuous supply of larvae for freshwater prawn farming ponds. However, studies involving the application of those techniques as well as on toxicity of cryoprotectants in freshwater prawn embryos are scarce. Thus, this study aims to test the toxicity of methylic alcohol (MET), dimethyl sulfoxide (DMSO) and ethylene glycol (EG) on Macrobrachium amazonicum embryos. For the present experiment, pools of embryos were taken from 15 M. amazonicum females and were divided into three groups and tested in duplicate at concentrations of 10, 5, 3; 1, 0.5 or 0.1%. Toxicity tests were conducted for 24 h in Falcon® pipes to obtain the lethal concentration for 50% of the larvae (LC50). After the set period for testing, random samples of embryos were removed for morphological analysis under stereoscopic microscopes. Results were analysed using analysis of variance (ANOVA) and Tukey's test at a 5% significance level and Trimmed Spearman-Karber Analysis to determine LC50-24 h. DMSO toxicity tests revealed that 5% and 10% concentrations showed the highest toxicity and differed from the control (P ≤ 0.05), 24h-LC50 was 437.4 ± 14.4 µL. MET was less toxic among the tested cryoprotectants and concentrations did not allow the determination of its LC50-24h. For tests with EG, concentrations of 3, 5 or 10% solutions resulted in a 100% mortality to tested embryos; EG was the tested cryoprotectant with the highest toxicity, with an LC50-24h average of 81.91 ± 35.3 µl.

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Reproductive cycle of Macrobrachium amazonicum females (Crustacea, Palaemonidae)

Brazilian Journal of Biology ◽

10.1590/s1519-69842007000300022 ◽

2007 ◽

Vol 67 (3) ◽

pp. 551-559 ◽

Cited By ~ 29

Author(s):

CMS. Sampaio ◽

RR. Silva ◽

JA. Santos ◽

SP. Sales

Keyword(s):

Environmental Parameters ◽

Sexual Cycle ◽

Freshwater Prawn ◽

Total Length ◽

In Captivity ◽

Maturation Stages ◽

Macrobrachium Amazonicum ◽

Monthly Sample ◽

Number Of Individuals ◽

Maturation Age

Macrobrachium amazonicum is considered a favorite Brazilian species of freshwater prawn for cultivation as a result of its quick development and because it is easy to maintain in captivity. The aim of this work is to describe the sexual cycle stages and determine maturation age of the female M. amazonicum, which was collected monthly from June, 2002 to May, 2003 in the Jaguaribe River, Itaiçaba, Ceará. A monthly sample of water was also collected to determine the following parameters: temperature, dissolved oxygen, pH and salinity. A monthly sample of females was selected among the individuals caught, to determine the total weight (W T), carapace length (L C) and abdomen+telson length (L A+T) and to register the number of non-ovigerous females (NOF) and ovigerous females (OF). Determining ovarian maturation stages of M. amazonicum was done in a laboratory by observing macroscopic characters such as coloring, size, location and appearance of ovarians examined by transparent carapace. The first maturation age was determined from the relative frequency of the total length (L T) of young and adult females. The environmental parameters of the Jaguaribe River did not hold any influence in the number of individuals collected. A total of 1,337 prawns were sampled, 513 males (38.4%) and 824 females (61.6%). The proportion between males and females in the studied population was of 1:1.6. Among the collected females, 492 (50.7%) did not carry eggs in their abdomens (NOF) and 332 (40.3%) carried eggs in their abdomens (OF). There was no record of intact females. Non-ovigerous females with mature ovaries were recorded throughout all the months of collection. The female ovaries were classified as immature (IM), rudimentary (RU), intermediary (IN) and mature (M). M. amazonicum females reach their first sexual maturity between 4.5 and 5.5 cm of total length.

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Histomorphologic characterization of the hepatopancreas of freshwater prawn Macrobrachium rosenbergii (De Man, 1879)

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-10497 ◽

2018 ◽

Vol 70 (5) ◽

pp. 1539-1546 ◽

Cited By ~ 1

Author(s):

M.A.S. Silva ◽

M.E. Almeida Neto ◽

B.O. Ramiro ◽

I.T.F. Santos ◽

R.R. Guerra

Keyword(s):

Macrobrachium Rosenbergii ◽

Distal Region ◽

Proximal Region ◽

Freshwater Prawn ◽

Cell Type ◽

Nutrient Storage ◽

Macrobrachium Amazonicum ◽

De Man ◽

First Time

ABSTRACT This study aimed to describe the Macrobrachium rosenbergii hepatopancreas histomorphology. The hepatopancreas is constituted by a set of blind end tubules, divided into proximal, middle, and distal regions, with the epithelium formed by E, F, B, R, and M cells differently of other species. Measurements of the length and width of the tubules were 419.64+69.09µm and 117.42+16.99µm, respectively. The percentage of each cell type per region was: proximal region (40%B, 20%F, 6.7%M, 33.3%R), middle region (45.4%B, 18.2%F, 9.1%M, and 27.3%R) and distal region (36.4%E, 27.2%B, 18.2%F, 9.1%M, 9.1%R). Cell B that stores glycogen and lipids, is the most commonly found cell in proximal and middle regions. In the distal region, the E, responsible for the mitosis, is the most prominent. M, responsible by nutrient storage, is numerically constant among the portions differently in the Macrobrachium amazonicum. The study for the first time also suggests that in addition to digestive enzymes, the F cell produces protective mucus. The present study generated for the first time a morphometric profile of M. rosenbergii hepatopancreas, demonstrating differences from other species, and can be an important tool for new studies in nutrition, reproduction, and production with the species.

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Morphometry, frequency and ultrastructure of male germ cells in morphotypes of the freshwater prawn Macrobrachium amazonicum (Decapoda: Palaemonidae)

Zoologischer Anzeiger ◽

10.1016/j.jcz.2018.11.002 ◽

2019 ◽

Vol 278 ◽

pp. 46-56 ◽

Cited By ~ 2

Author(s):

Gicelle M.F. Silva ◽

Yanne A. Mendes ◽

Ivana K.S. Viana ◽

Liziane A.B. Gonçalves ◽

Renata S. Oliveira ◽

...

Keyword(s):

Germ Cells ◽

Freshwater Prawn ◽

Male Germ Cells ◽

Macrobrachium Amazonicum

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Isolation and characterization of SNPs within HSC70 gene in the freshwater prawn Macrobrachium amazonicum

Conservation Genetics Resources ◽

10.1007/s12686-013-9867-y ◽

2013 ◽

Vol 5 (3) ◽

pp. 631-633 ◽

Cited By ~ 1

Author(s):

Danielly Veloso Blanck ◽

Wagner C. Valenti ◽

Patrícia Domingues de Freitas ◽

Pedro Manoel Galetti Junior

Keyword(s):

Freshwater Prawn ◽

Isolation And Characterization ◽

Macrobrachium Amazonicum

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Fecundity and fertility of Macrobrachium amazonicum (Crustacea, Palaemonidae)

Brazilian Journal of Biology ◽

10.1590/s1519-69842004000300012 ◽

2004 ◽

Vol 64 (3a) ◽

pp. 489-500 ◽

Cited By ~ 19

Author(s):

R. R. da Silva ◽

C. M. S. Sampaio ◽

J. A. Santos

Keyword(s):

Linear Relationship ◽

Macrobrachium Rosenbergii ◽

High Volume ◽

Total Weight ◽

Freshwater Prawn ◽

Total Length ◽

Average Fecundity ◽

Develop Technology ◽

Macrobrachium Amazonicum ◽

Average Fertility

In Brazil, studies with native freshwater prawn species were discontinued due to great importance of Macrobrachium rosenbergii. Therefore, it is necessary to continue investigations about our species, in order to develop technology adequate to our reality and in a future allow prawn farmers to culture other species. The aim of this study was to determine the fecundity and fertility of Macrobrachium amazonicum captured monthly from June 1999 to June 2001 from Jaguaribe River, Itaiçaba, Ceará, Brazil. Prawns were collected using fishing net and transferred at Biological Science Laboratory, Ceará State University (Fortaleza, Ceará, Brazil). Among the ovigerous M. amazonicum females, 60 were randomly selected to determine fecundity. The eggs adhered to the pleopods were taken out and they were then placed in a Gilson solution, and then stored in alcohol 70%. Individual fecundity was determined from the total egg counting, using a stereoscopic microscope. To determine fertility, ovigerous M. amazonicum females were stored in individual 10 L-glass tanks maintained under strong aeration. After the hatching, the larvae were siphoned and counted. The data referring to total length and weight of all the females, storage date, coloration and number of eggs, weight and coloration of gonad and number of hatched larvae were noted. With respect to average fecundity (F) by length classes, the lowest and highest number of eggs observed was 696 and 1,554, respectively. As for fecundity by weight classes, the lowest number of eggs observed was 760 and the highest, 1,690. The highest number of eggs observed individually per hatching was 2,193. Average fecundity/total length (L) and average fecundity/total weight (W) may be expressed by a linear relationship. The adjusted equations are: F = -411.6 + 203.1 L (p < 0.0001) and F = 566.4 + 157.3 W (p < 0.0001), respectively. In the analysis of average fertility (N) per length classes, the lowest and highest number of larvae observed was 374 and 1,301, respectively. With respect to fertility per weight classes, the lowest number of larvae was 581 and the highest, 1,391. In this work, the maximum number of larvae observed per hatching for females kept in laboratory was 2,594. Average fertility/total length and average fertility/total weight may be expressed by a linear relationship. The adjusted equations are: N = -1042.7 + 264.9 L (p < 0.0002); N = 384.1 + 160.3 W (p < 0.003). From these results we can deduced that fecundity and fertility of M. amazonicum are lower than ones commercial species, nevertheless it can be captured during all year long. The number of captured prawns was large in the months whose level of water was low; the inverse was observed in the months that the river was with a high volume of water.

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Genetic variability of the Amazon River prawn Macrobrachium amazonicum (Decapoda, Caridea, Palaemonidae)

Contributions to Zoology ◽

10.1163/18759866-08001003 ◽

2011 ◽

Vol 80 (1) ◽

pp. 67-83 ◽

Cited By ~ 50

Author(s):

Fernanda G. Vergamini ◽

Leonardo G. Pileggi ◽

Fernando L. Mantelatto

Keyword(s):

Genetic Divergence ◽

Natural Populations ◽

Intraspecific Variability ◽

Unknown Origin ◽

Single Species ◽

Northeastern Brazil ◽

Freshwater Prawn ◽

Mato Grosso ◽

Wide Range ◽

Macrobrachium Amazonicum

The freshwater prawn Macrobrachium amazonicum is widely distributed in South America, and occupies habitats with a wide range of salinities. Several investigations have revealed the existence of wide intraspecific variability among different populations, although the understanding of this variability is still fragmentary and incomplete. We compared and characterized inland and coastal populations of M. amazonicum from Brazil, using molecular data (16S and COI mtDNA) to describe the degree of variability, structure, and relationships among them. Genetic divergence rates among populations showed variability at the intraspecific level. All the analyses evidenced significant genetic divergence among populations, structuring them in three groups: I- inland waters of the Amazonian Hydrographic Region (HR); II- Paraná/Paraguay HR; and III- coastal systems of northern and northeastern Brazil. Phylogenetic reconstructions revealed that the populations form a single monophyletic clade, which supports their characterization as a single species. Clade I was a sister clade of that formed by clades II and III, which were themselves sister clades. Populations from Sertãozinho/ Miguelópolis and Avaré, introduced into the state of São Paulo, may have originated from natural populations in the states of Mato Grosso do Sul and Pará, respectively. Geographical isolation probably contributed to the observed variation, and if this isolation continues, M. amazonicum may undergo speciation within its broad geographical distribution. The sequences obtained here can be used as name-tags for population identification, and the DNA barcodes are useful to identify the origin of specimens used in different freshwater-prawn cultures or introduced populations of unknown origin.

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