scholarly journals DEMONSTRATION OF ACID PHOSPHATASE-CONTAINING GRANULES AND CYTOPLASMIC BODIES IN THE EPITHELIUM OF FOETAL RAT DUODENUM DURING CERTAIN STAGES OF DIFFERENTIATION

1963 ◽  
Vol 18 (2) ◽  
pp. 251-265 ◽  
Author(s):  
O. Behnke
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Parallel Study ◽  
Frozen Sections ◽  
Cytoplasmic Bodies ◽  
Dense Bodies ◽  
Foetal Rat ◽  
Rat Duodenum ◽  
Myelin Figures

Dense cytoplasmic bodies surrounded by one or two unit membranes and containing mitochondria, vesicles, ribosomes, rough and smooth surfaced endoplasmic reticulum, and lamellated membranes (myelin figures) have been observed in the differentiating mucosa of the duodenum of rat foetuses by electron microscopy. Generally, the cytoplasmic components in the bodies seem to be in varying stages of disintegration. The bodies are found in greatest number on the 17th and 18th day of gestation, i.e. at the onset of differentiation. At this period of development the epithelium is stratified, and the villus formation is initiated by invagination of the epithelium by buds of mesenchyme followed by a splitting of the epithelium along the sides of the invaginations. When the villi have formed, the stratified epithelium has changed to the simple columnar type and the dense bodies have largely disappeared. Simultaneously, the lumen has widened considerably. In a parallel study with the light microscope, frozen sections incubated for the demonstration of acid phosphatase activity revealed the reaction product to be localized in bodies of the same size and distribution as the dense bodies found by electron microscopy. Hence, it seems that the bodies are altered and enlarged lysosomes (cytolysomes) active during the intensive differentiative events in the small intestine during the last part of intra-uterine life.

Copper Localization in Cirrhotic Rat Liver by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 38-39 ◽  
Author(s):  
J. C. Russ ◽  
E. McNatt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Copper Acetate ◽  
Lead Citrate ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Electron Mode ◽  
Scanning Electron

In order to study the retention of copper in cirrhotic liver, rats were made cirrhotic by carbon tetrachloride inhalation twice weekly for three months and fed 0.2% copper acetate ad libidum in drinking water for one month. The liver tissue was fixed in osmium, sectioned approximately 2000 Å thick, and stained with lead citrate. The section was examined in a scanning electron microscope (JEOLCO JSM-2) in the transmission electron mode.Figure 1 shows a typical area that includes a red blood cell in a sinusoid, a disse, and a portion of the cytoplasm of a hepatocyte which contains several mitochondria, peribiliary dense bodies, glycogen granules, and endoplasmic reticulum.

Formation of chlamydospores in Gilbertella persicaria

1981 ◽  
Vol 59 (5) ◽  
pp. 908-928 ◽  
Author(s):  
Martha J. Powell ◽  
Charles E. Bracker ◽  
David J. Sternshein
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Acid Phosphatase ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Marker Enzyme ◽  
Transparent Layer ◽  
Thiamine Pyrophosphatase ◽  
Gilbertella Persicaria

The cytological events involved in the transformation of vegetative hyphae of the zygomycete Gilbertella persicaria (Eddy) Hesseltine into chlamydospores were studied with light and electron microscopy. Thirty hours after sporangiospores were inoculated into YPG broth, swellings appeared along the aseptate hyphae. Later, septa, traversed by plasmodesmata, delimited each end of the hyphal swellings and compartmentalized these hyphal regions as they differentiated into chlamydospores. Nonswollen regions adjacent to chlamydospores remained as isthmuses. Two additional wall layers appeared within the vegetative wall of the developing chlamydospores. An alveolate, electron-dense wall formed first, and then an electron-transparent layer containing concentrically oriented fibers formed between this layer and the plasma membrane. Rather than a mere condensation of cytoplasm, development and maturation of the multinucleate chlamydospores involved extensive cytoplasmic changes such as an increase in reserve products, lipid and glycogen, an increase and then disappearance of vacuoles, and the breakdown of many mitochondria. Underlying the plasma membrane during chlamydospore wall formation were endoplasmic reticulum, multivesicular bodies, vesicles with fibrillar contents, vesicles with electron-transparent contents, and cisternal rings containing the Golgi apparatus marker enzyme, thiamine pyrophosphatase. Acid phosphatase activity was localized cytochemically in a cisterna which enclosed mitochondria and in vacuoles which contained membrane fragments. Tightly packed membrane whorls and single membrane bounded sacs with finely granular matrices surrounding vacuoles were unique during chlamydospore development. Microbodies were rare in the mature chlamydospore, but endoplasmic reticulum was closely associated with lipid globules. As chlamydospores developed, the cytoplasm in the isthmus became highly vacuolated, lipid globules were closely associated with vacuoles, mitochondria were broken down in vacuoles, unusual membrane configurations appeared, and eventually the membranes degenerated. Unlike chlamydospores, walls of the isthmus did not thicken, but irregularly shaped appositions containing numerous channels formed at intervals on the inside of these walls. The pattern of cytoplasmic transformations during chlamydospore development is similar to events leading to the formation of zygospores and sporangiospores.

scholarly journals LOCALIZATION OF PEROXIDASE ACTIVITY IN MICROBODIES OF FETAL MOUSE LIVER

1969 ◽  
Vol 17 (7) ◽  
pp. 454-466 ◽  
Author(s):  
EDWARD ESSNER
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Peroxidase Activity ◽  
Mouse Liver ◽  
Fetal Liver ◽  
Light And Electron Microscopy ◽  
Small Population ◽  
Fetal Mouse ◽  
Fetal Mouse Liver

The peroxidase activity of microbodies in fetal mouse liver was studied by light and electron microscopy. Two types of microbodies were present; a small population of bodies that lacked a nucleoid, predominant on the 16th day of gestation, and a larger population of nucleoid-bearing microbodies, predominant on the 19th day, in association with the rough endoplasmic reticulum from which they probably originate. Both types of bodies were visualized when incubated for peroxidase activity but were negative (19th day) for acid phosphatase activity. The findings suggest that the anucleoid- and nucleoid-bearing organelles together constitute the microbody population of the fetal liver.

scholarly journals CYTOCHEMICAL STUDIES OF LYSOSOMES, GOLGI APPARATUS AND ENDOPLASMIC RETICULUM IN SECRETION AND PROTEIN UPTAKE BY ADRENAL MEDULLA CELLS OF THE RAT

1968 ◽  
Vol 16 (5) ◽  
pp. 320-336 ◽  
Author(s):  
ERIC HOLTZMAN ◽  
REGINA DOMINITZ
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Golgi Apparatus ◽  
Adrenal Medulla ◽  
Secretory Granules ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Frozen Sections ◽  
Protein Uptake ◽  
Thiamine Pyrophosphatase

The adrenalin-producing cells of the rat adrenal medulla have been studied by light and electron microscopy. Frozen sections of glutaraldehyde-perfused material were incubated for demonstration of "marker" enzymes for lysosomes (acid phosphatase, aryl sulfatase) and Golgi apparatus (thiamine pyrophosphatase). In addition, the uptake and fate of intravenously administered horseradish peroxidase was followed. Acid phosphatase activity is demonstrable in secretory granules, Golgi saccules, vesicles in the Golgi area and in the agranular tubules and cisternae (GERL) from which secretory granules appear to form at the inner surface of the Golgi apparatus. Endoplasmic reticulum with ribosomes on only one surface is closely apposed to both inner and outer aspects of the Golgi apparatus. Peroxidase is taken up in vesicles, tubules and "cup-like" bodies. The latter apparently transform into multivesicular bodies. A possible source of the acid phosphatase found in multivesicular bodies is the small vesicles from the Golgi apparatus or GERL.

scholarly journals Canine Cutaneous Histiocytoma A Light and Electron Microscopic Study

1970 ◽  
Vol 7 (1) ◽  
pp. 12-27 ◽  
Author(s):  
D. F. Kelly
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Acid Phosphatase ◽  
Microscopic Study ◽  
Mononuclear Cell ◽  
Electron Microscopic Study ◽  
Light And Electron Microscopy ◽  
Perinuclear Region ◽  
Electron Microscopic

Cutaneous histiocytomas from 4 dogs were examined by light and electron microscopy. A large (up to 10 μ in diameter) mononuclear cell with prominent filiform processes of the plasma membrane predominated. Its cytoplasm contained relatively small amounts of endoplasmic reticulum and mitochondria, only occasional lysosomes, fibrils, most obvious in the perinuclear region, and small amounts of cytoplasmic debris. Acid phosphatase was not detected. Fibroblasts and collagen formed a small part of the lesion, except at the junction with surrounding dermis, where fibers were plentiful. The morphologic features of the lesion are compatible with the suggestion that the predominant cell is of histiocytic type.

scholarly journals THE LOCALIZATION OF ACID PHOSPHATASE IN RAT LIVER CELLS AS REVEALED BY COMBINED CYTOCHEMICAL STAINING AND ELECTRON MICROSCOPY

1961 ◽  
Vol 11 (1) ◽  
pp. 47-66 ◽  
Author(s):  
S. J. Holt ◽  
R. Marian Hicks
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Light Microscope ◽  
Staining Pattern ◽  
Dense Body ◽  
Butyl Methacrylate ◽  
Nuclear Staining ◽  
Heterogeneous Distribution ◽  
Dense Bodies ◽  
Before And After

Discrete localization of stain in pericanalicular granules was found in 10 µ frozen sections of formol-phosphate-sucrose-fixed liver stained by the Gomori acid phosphatase technique and examined in the light microscope. The staining patterns, before and after treatment with Triton X-100 and lecithinase, were identical with those previously reported for formol-calcium-fixed material treated in the same way, and it can be assumed that the stained granules are identical with "lysosomes." Examination in the light microscope of the staining patterns and lead penetration in fixed blocks and slices of various dimensions showed nuclear staining and other artefacts to be present, produced by the different rates of penetration of the various components of the staining medium into the tissue. A uniform pericanalicular staining pattern could be obtained, however, with slices not more than 50 µ thick, into which the staining medium could penetrate rapidly from both faces. The staining pattern produced in 50 µ slices was the same both at pH 5.0 and pH 6.2, and was not altered by subsequent embedding of the stained material in butyl methacrylate. Electron microscopy showed the fine structure of fixed 50 µ frozen slices to be well preserved, but it deteriorated badly when they were incubated in the normal Gomori medium at pH 5.0 before postfixing in osmium tetroxide. After incubation in the Gomori medium at pH 6.2, the detailed morphology was substantially maintained. In both cases lead phosphate, the reaction product, was found in the pericanalicular regions of the cell, but only in the vacuolated dense bodies and never in the microbodies. Not every vacuolated dense body contained lead, and stained and unstained bodies were sometimes seen adjacent to each other. This heterogeneous distribution of stain within a morphologically homogeneous group of particles is consistent with de Duve's suggestion (9) that there is a heterogeneous distribution of enzymes within the lysosome population. It is concluded from these investigations that the vacuolated dense bodies seen in the electron microscope are the morphological counterparts of the "lysosomes" defined biochemically by de Duve.

The York Platelet Syndrome: A Third Case and New Findings

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 2526-2526
Author(s):  
James G White ◽  
Regents Professor ◽  
Merel Gunay-Aygun
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Golgi Complex ◽  
Analytical Electron Microscopy ◽  
Common Finding ◽  
High Dose ◽  
Dense Bodies ◽  
New Findings ◽  
Whole Mount ◽  
Analytical Electron

Abstract Abstract 2526 Studies published in 2003 described a mother and son with a mitochondrial myopathy and abnormal platelets containing giant electron opaque organelles and large, multilayered organelles resembling targets. The condition has remained a familial disorder until recently when a new patient, a four year old female, with the same myopathy and platelet ultrastructure was discovered. Now the disorder can be considered a syndrome named after the initial family, the York Platelet Syndrome (YPS). Whole mount preparations of unfixed, unstained YPS platelets revealed that the giant opaque organelles (OO) were as inherently electron dense as delta granules (dense bodies, DB) but several times larger. Analytical electron microscopy revealed both organelles were rich in phosphorous and calcium, suggesting they might have the same origin. However, measurements of serotonin and adenine nucleotides in YPS platelets revealed normal levels. Also, staining YPS platelets for acid phosphatase with cerium as the capture ion and using analytical electron microscopy to determine its location in whole mount preparations revealed the presence of cerium in the giant OO, but not in DB. The findings suggested the OO might be giant secretory organelles. However, exposure of YPS platelets to high dose thrombin caused complete secretion of α granules and DB, but OO and TO were not released through the open canalicular system or the surface membrane. Thus, OO and TO are not secretory organelles. Studies of YPS in the new patient have confirmed and extended these findings. Staining her cells with diaminobenzidine and H202 to detect platelet peroxidase has revealed enzyme reaction product in both OO and TO, as well as in channels of the dense tubular system. Thus, the giant OO and TO organelles contain proteins and elements usually separated into vesicles in the Golgi Complex and reassembled into alpha granules, dense bodies and lysosomes in megakaryocytes. Instead proteins and elements from the rough and smooth endoplasmic reticulum (SER) develop into giant OO and TO without passing through lamellae of the Golgi complex. Involvement of the SER and DTS in formation of the giant organelles is strongly supported by YPS platelets from the new patient. Large, flat sheets and coils of SER were a common finding in the new YPS patient platelets, and present, but less frequent in the original two YPS patients. The findings strongly support the concept that the YPS in a disorder of megakaryocyte endoplasmic reticulum allowing the formation OO and TO which continue to develop in the DTS of YPS circulating platelets. It is the first such disorder to be described in human megakaryocytes and platelets. Disclosures: No relevant conflicts of interest to declare.

scholarly journals FINE STRUCTURE OBSERVATIONS OF THE UPTAKE OF INTRAVENOUSLY INJECTED PEROXIDASE BY THE RAT CHOROID PLEXUS

1967 ◽  
Vol 15 (3) ◽  
pp. 160-165 ◽  
Author(s):  
NORWIN H. BECKER ◽  
ALEX B. NOVIKOFF ◽  
H. M. ZIMMERMAN
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Cerebrospinal Fluid ◽  
Choroid Plexus ◽  
Smooth Endoplasmic Reticulum ◽  
Multivesicular Bodies ◽  
Adult Rats ◽  
Dense Bodies ◽  
Pinocytotic Vesicles ◽  
Membrane Bound

The uptake by the choroid plexus of adult rats of intravenously injected horseradish peroxidase has been investigated by electron microscopy. Within 4 min, the injected protein passes the capillary and is rapidly distributed through extracellular space and choroidal cells. Peroxidase enters the choroidal cells within coated vesicles which act as pinocytotic vesicles. At 15 min, peroxidase activity is present in numerous membrane-bound vesicles, multivesicular bodies, dense bodies and what appear to be segments of smooth endoplasmic reticulum. None of the peroxidase-containing organelles is seen to empty to the ventricular surface. Egress of the extracellular peroxidase into the cerebrospinal fluid is apparently blocked by apical zonulae occludentes between the choroidal cells.

scholarly journals ELECTRON MICROSCOPIC LOCALIZATION OF GLYCOPROTEINS IN PITUITARY CELLS OF DUCK AND QUAIL

1971 ◽  
Vol 19 (12) ◽  
pp. 775-797 ◽  
Author(s):  
ANDRÉE TIXIER-VIDAL ◽  
RENÉE PICART
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Secretory Granules ◽  
Periodic Acid ◽  
Pituitary Cells ◽  
Electron Microscopic ◽  
Gonadotropic Cells ◽  
Dense Bodies

Structures demonstrating the presence of glycoproteins, acid phosphatase activity and OsO4 impregnation were localized by means of the electron microscope in duck and in quail pituitary cells. Two methods for the electron microscopic demonstration of glycoproteins were used: a chromic acid-phosphotungstic acid mixture on glycol-methacrylate-embedded tissues, and the periodic acid-thiocarbohydrazide-silver proteinate technique. Both methods showed glycoproteins in the following sites: ( a) the secretory granules in three types of cells (A, B, C) which are part of the seven different cells of the avian pituitary; ( b) the several kinds of dense bodies which are richer in reaction product than the secretory granules. A correlation with previous studies on similar species of birds is helpful in identifying each of the three positive types of cells as thyrotropic cell (A), prolactin cell (B) and gonadotropic cell (C). The presence of glycoproteins within the Golgi saccules (on condensing granules) was found with the periodic acid-thiocarbohydrazide-silver proteinate method in these gonadotropic cells only. In gonadotropic and thyrotropic cells, acid phosphatase activity is weak in the inner Golgi saccules and strong in the "Golgi Endoplasmic Reticulum Lysosomes" system, in the lysosomes, in the dense bodies and in the vacuolated dense bodies. The structures which are richest in glycoproteins are also those which have the most acid phosphatase activity. On the contrary, OsO4-stained structures in duck gonadotropic cells (nuclear pericisterna, rough endoplasmic reticulum, cisternae and outer Golgi saccules) have no glycoproteins or acid phosphatase activity.

Ultrastructure of Solitary Enchondromas

1984 ◽  
Vol 9 (1) ◽  
pp. 95-97 ◽  
Author(s):  
MARILYN L. ZIMNY ◽  
I. REDLER
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Cell Types ◽  
Transmission Electron ◽  
The Matrix ◽  
Small Bones ◽  
Dying Cells ◽  
Myelin Figures

Solitary enchondromas obtained from the small bones of the hand were studied with transmission electron microscopy. Three cell types were seen as follows: (1) young looking, active cells with extensive dilated rough endoplasmic reticulum and well defined Golgi and mitochondria; (2) older looking, degenerating cells with dilated rough endoplasmic reticulum, well defined Golgi, glycogen masses, vacuoles containing tropocollagen, lipid and myelin figures; and (3) dying cells showing loss of cell membrane and lysosomal-like bodies. A young chondroblastic cell may try to mature, become a normal chondrocyte that produces normal matrix but it does not succeed and dies. Enchondromal cells are not capable of forming tropocollagen or synthesizing proteoglycans for the matrix.

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