scholarly journals HOMOGENEOUS RABBIT 7S ANTI-IGG WITH ANTIBODY SPECIFICITY FOR PEPTIDOGLYCAN

1973 ◽  
Vol 138 (5) ◽  
pp. 1184-1193 ◽  
Author(s):  
Viktor A. Bokisch ◽  
Jen Wei Chiao ◽  
David Bernstein ◽  
Richard M. Krause
Keyword(s):  
Cell Wall ◽  
Antigenic Determinant ◽  
Antibody Specificity ◽  
Streptococcal Cell Wall ◽  
Cell Wall Peptidoglycan ◽  
Precipitin Reaction ◽  
Antigen Antibody ◽  
The Relationship

The relationship between 7S anti-IgG and antibodies to streptococcal cell wall peptidoglycan was examined for four streptococcal Group C antisera. Homogeneous 7S anti-IgG components in these sera were isolated by means of an IgG immunoadsorbent column. For two of the four antisera, the anti-peptidoglycan activity of the 7S anti-IgG had specificity for the pentapeptide, L-Ala-D-Glu-γ-L-Lys-D-Ala-D-Ala, the antigenic determinant of peptidoglycan, as well as for the Fc of IgG. Detailed studies on the 7S anti-IgG from one of the antisera revealed that the pentapeptide inhibited the coprecipitation reaction of 7S anti-IgG R3387 with antigen-antibody complexes and the precipitin reaction between 7S anti-IgG R3387 and its anti-idiotype serum.

scholarly journals THE LYSIS OF GROUP A HEMOLYTIC STREPTOCOCCI BY EXTRACELLULAR ENZYMES OF STREPTOMYCES ALBUS

1952 ◽  
Vol 96 (6) ◽  
pp. 569-580 ◽  
Author(s):  
Maclyn McCarty
Keyword(s):  
Cell Wall ◽  
Extracellular Enzymes ◽  
Group A Streptococci ◽  
Carbohydrate Component ◽  
Streptococcal Cell Wall ◽  
Intact Cells ◽  
Enzymatic Lysis ◽  
Streptomyces Albus ◽  
Group A ◽  
Isolated Cell

Cell wall preparations of uniform chemical constitution have been obtained from several strains of group A streptococci. The isolated cell walls are dissolved by the same fractions of the Streptomyces albus enzymes that are effective in the lysis of intact cells, and it is likely that enzymatic lysis of group A streptococci is effected by an attack on the cell wall. The streptococcal cell wall, as prepared in this study, consists of approximately two-thirds carbohydrate and one-third protein. Small amounts of other components may be present. The carbohydrate component, which is composed primarily of N-acetyl-glucosamine and rhamnose, is the group-specific C carbohydrate. The evidence indicates that one of the streptomyces enzymes is directed toward the carbohydrate component of the cell wall.

Targeting Bacterial Cell Wall Peptidoglycan Synthesis by Inhibition of Glycosyltransferase Activity

2015 ◽  
Vol 87 (2) ◽  
pp. 190-199 ◽  
Author(s):  
Michael F. Mesleh ◽  
Premraj Rajaratnam ◽  
Mary Conrad ◽  
Vasu Chandrasekaran ◽  
Christopher M. Liu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Peptidoglycan Synthesis ◽  
Cell Wall Peptidoglycan
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