The Onset Temperature of the Heat-Shock Response and Whole-Organism Thermal Tolerance Are Tightly Correlated in both Laboratory-Acclimated and Field-Acclimatized Tidepool Sculpins (Oligocottus maculosus)

2011 ◽  
Vol 84 (4) ◽  
pp. 341-352 ◽  
Author(s):  
Nann A. Fangue ◽  
Edward J. Osborne ◽  
Anne E. Todgham ◽  
Patricia M. Schulte
Keyword(s):  
Heat Shock ◽  
Heat Shock Response ◽  
Thermal Tolerance ◽  
Onset Temperature ◽  
Shock Response ◽  
Oligocottus Maculosus

Heat shock response of killifish (Fundulus heteroclitus): candidate gene and heterologous microarray approaches

2010 ◽  
Vol 41 (2) ◽  
pp. 171-184 ◽  
Author(s):  
Timothy M. Healy ◽  
Wendy E. Tymchuk ◽  
Edward J. Osborne ◽  
Patricia M. Schulte
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Shock Response ◽  
Thermal Tolerance ◽  
Fundulus Heteroclitus ◽  
Swimming Performance ◽  
Shock Response ◽  
Shock Proteins ◽  
Heterologous Microarray

Northern and southern subspecies of the Atlantic killifish, Fundulus heteroclitus, differ in maximal thermal tolerance. To determine whether these subspecies also differ in their heat shock response (HSR), we exposed 20°C acclimated killifish to a 2 h heat shock at 34°C and examined gene expression in fish from both subspecies during heat shock and recovery using real-time quantitative PCR and a heterologous cDNA microarray designed for salmonid fishes. The heat shock proteins Hsp70-1, hsp27, and hsp30 were upregulated to a greater extent in the high temperature-tolerant southern subspecies than in the less tolerant northern subspecies, whereas hsp70-2 (which showed the largest upregulation of all the heat shock proteins) in both gill and muscle and hsp90α in muscle was upregulated to a greater extent in northern than in southern fish. These data demonstrate that differences in the HSR between subspecies cannot be due to changes in a single global regulator but must occur via gene-specific mechanisms. They also suggest that the role, if any, of hsps in establishing thermal tolerance is complex and varies from gene to gene. Heterologous microarray hybridization provided interpretable gene expression signatures, detecting differential regulation of genes known to be involved in the heat shock response in other species. Under control conditions, a variety of genes were differentially expressed in muscle between subspecies that suggest differences in muscle fiber type and could relate to previously observed differences between subspecies in the thermal sensitivity of swimming performance and metabolism.

Plasticity and acquisition of the thermal tolerance (upper thermal limit and heat shock response) in the intertidal species Palaemon elegans

2016 ◽  
Vol 484 ◽  
pp. 39-45 ◽  
Author(s):  
Juliette Ravaux ◽  
Nelly Léger ◽  
Nicolas Rabet ◽  
Claire Fourgous ◽  
Guillaume Voland ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Response ◽  
Thermal Tolerance ◽  
Thermal Limit ◽  
Intertidal Species ◽  
Shock Response ◽  
Palaemon Elegans ◽  
Upper Thermal Limit

Effects of desiccation and starvation on thermal tolerance and the heat-shock response in forest ants

2017 ◽  
Vol 187 (8) ◽  
pp. 1107-1116 ◽  
Author(s):  
Andrew D. Nguyen ◽  
Kerri DeNovellis ◽  
Skyler Resendez ◽  
Jeremy D. Pustilnik ◽  
Nicholas J. Gotelli ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Response ◽  
Thermal Tolerance ◽  
Shock Response

scholarly journals Stressed out underground?: Transcriptomics illuminates the genome-wide response to heat stress in surface and subterranean diving beetles

2021 ◽  
Author(s):  
Perry G Beasley-Hall ◽  
Terry Bertozzi ◽  
Tessa M Bradford ◽  
Charles S P Foster ◽  
Karl Jones ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Response ◽  
Thermal Tolerance ◽  
Molecular Level ◽  
Differential Expression Analysis ◽  
Increased Risk ◽  
Shock Response ◽  
The Face ◽  
Surface Dwelling

Subterranean habitats are environmentally stable with respect to temperature, humidity, and the absence of light. The transition to a subterranean lifestyle might therefore be expected to cause considerable shifts in an organism's physiology; here, we investigate how subterranean colonisation affects thermal tolerance. Subterranean organisms might be at an increased risk of decline in the face of global temperature rises, but robust data on the fauna is lacking, particularly at the molecular level. In this study we compare the heat shock response of two species of diving beetle in the genus Paroster: one surface-dwelling (P. nigroadumbratus), the other restricted to a single aquifer (P. macrosturtensis). P. macrosturtensis has been previously established as having a lower thermal tolerance compared to surface-dwelling relatives, but the genomic basis of this difference is unknown. By sequencing transcriptomes of experimentally heat-shocked individuals and performing differential expression analysis, we demonstrate both species can mount a heat shock response at high temperatures (35C), in agreement with past survival experiments. However, the genes involved in these responses differ between species, and far greater genes are differentially expressed in the surface species, which may explain its more robust response to heat stress. In contrast, the subterranean species significantly upregulated the heat shock protein gene Hsp68 in the experimental setup under conditions it would likely encounter in nature (25C), suggesting it may be more sensitive to ambient stressors, e.g. handling. The results presented here contribute to an emerging narrative concerning weakened thermal tolerances in obligate subterranean organisms at the molecular level.

Sign in / Sign up

Export Citation Format

Share Document