Nutrient Uptake by Marine Invertebrates: Cloning and Functional Analysis of Amino Acid Transporter Genes in Developing Sea Urchins (Strongylocentrotus purpuratus)

2009 ◽  
Vol 217 (1) ◽  
pp. 6-24 ◽  
Author(s):  
Eli Meyer ◽  
Donal T. Manahan
Keyword(s):  
Functional Analysis ◽  
Amino Acid ◽  
Nutrient Uptake ◽  
Marine Invertebrates ◽  
Sea Urchins ◽  
Strongylocentrotus Purpuratus ◽  
Amino Acid Transporter ◽  
Acid Transporter

scholarly journals Functional analysis of human aromatic amino acid transporter MCT10/TAT1 using the yeast Saccharomyces cerevisiae

2017 ◽  
Vol 1859 (10) ◽  
pp. 2076-2085 ◽  
Author(s):  
Satoshi Uemura ◽  
Takahiro Mochizuki ◽  
Goyu Kurosaka ◽  
Takanori Hashimoto ◽  
Yuki Masukawa ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Analysis ◽  
Amino Acid ◽  
Aromatic Amino Acid ◽  
Amino Acid Transporter ◽  
Yeast Saccharomyces Cerevisiae ◽  
Acid Transporter

scholarly journals Effects of arginine on intestinal epithelial cell integrity and nutrient uptake

2016 ◽  
Vol 116 (10) ◽  
pp. 1675-1681 ◽  
Author(s):  
Mi Xia ◽  
Lulu Ye ◽  
Qihang Hou ◽  
Qinghua Yu
Keyword(s):  
Amino Acid ◽  
Epithelial Cell ◽  
Nutrient Uptake ◽  
Intestinal Epithelial Cell ◽  
Amino Acid Transporter ◽  
Acid Uptake ◽  
Intestinal Epithelial ◽  
Physiological Level ◽  
Acid Transporter ◽  
Arginine Concentration

AbstractArginine is a multifaceted amino acid that is critical to the normal physiology of the gastrointestinal tract. Oral arginine administration has been shown to improve mucosal recovery following intestinal injury. The present study investigated the influence of extracellular arginine concentrations on epithelial cell barrier regulation and nutrition uptake by porcine small intestinal epithelial cell line (IPEC-J2). The results show that reducing arginine concentration from 0·7 to 0·2 mm did not affect the transepithelial electrical resistance value, tight-junction proteins (claudin-1, occludin, E-cadherin), phosphorylated extracellular signal-regulated protein kinases (p-ERK) and mucin-1 expression. Furthermore, reducing arginine concentration stimulated greater expression of cationic amino acid transporter (CAT1), excitatory amino acid transporter (EAAT3) and alanine/serine/cysteine transporter (ASCT1) mRNA by IPEC-J2 cells, which was verified by elevated efficiency of amino acid uptake. Glucose consumption by IPEC-J2 cells treated with 0·2 mm-arginine remained at the same physiological level to guarantee energy supply and to maintain the cell barrier. This experiment implied that reducing arginine concentration is feasible in IPEC-J2 cells guaranteed by nutrient uptake and cell barrier function.

Increased expression of an amino acid transporter LAT1 in healing of acetic acid-induced chronic gastric ulcer in rats

2001 ◽  
Vol 120 (5) ◽  
pp. A153-A153
Author(s):  
S MIYAMOTO ◽  
K KATO ◽  
Y ISHII ◽  
S ASAI ◽  
T NAGAISHI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Amino Acid ◽  
Amino Acid Transporter ◽  
Chronic Gastric Ulcer ◽  
Acid Transporter

scholarly journals Identification and Characterization of Inhibitors of a Neutral Amino Acid Transporter, SLC6A19, Using Two Functional Cell-Based Assays

2018 ◽  
Vol 24 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Sanjay J. Danthi ◽  
Beirong Liang ◽  
Oanh Smicker ◽  
Benjamin Coupland ◽  
Jill Gregory ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Throughput Screening ◽  
Amino Acid Transporter ◽  
Neutral Amino Acid ◽  
Imaging Plate ◽  
Acid Uptake ◽  
Functional Assays ◽  
Neutral Amino Acid Transporter ◽  
Acid Transporter ◽  
Pharmacologically Active

SLC6A19 (B0AT1) is a neutral amino acid transporter, the loss of function of which results in Hartnup disease. SLC6A19 is also believed to have an important role in amino acid homeostasis, diabetes, and weight control. A small-molecule inhibitor of human SLC6A19 (hSLC6A19) was identified using two functional cell-based assays: a fluorescence imaging plate reader (FLIPR) membrane potential (FMP) assay and a stable isotope-labeled neutral amino acid uptake assay. A diverse collection of 3440 pharmacologically active compounds from the Microsource Spectrum and Tocriscreen collections were tested at 10 µM in both assays using MDCK cells stably expressing hSLC6A19 and its obligatory subunit, TMEM27. Compounds that inhibited SLC6A19 activity in both assays were further confirmed for activity and selectivity and characterized for potency in functional assays against hSLC6A19 and related transporters. A single compound, cinromide, was found to robustly, selectively, and reproducibly inhibit SLC6A19 in all functional assays. Structurally related analogs of cinromide were tested to demonstrate structure–activity relationship (SAR). The assays described here are suitable for carrying out high-throughput screening campaigns to identify modulators of SLC6A19.

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