Measuring protein dynamics with ultrafast two-dimensional infrared spectroscopy

2012 ◽  
Vol 23 (6) ◽  
pp. 062001 ◽  
Author(s):  
Katrin Adamczyk ◽  
Marco Candelaresi ◽  
Kirsty Robb ◽  
Andrea Gumiero ◽  
Martin A Walsh ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Protein Dynamics ◽  
Two Dimensional ◽  
Two Dimensional Infrared Spectroscopy

Protein Dynamics by Two-Dimensional Infrared Spectroscopy

2021 ◽  
Vol 14 (1) ◽  
pp. 299-321
Author(s):  
Goran W. Tumbic ◽  
Md Yeathad Hossan ◽  
Megan C. Thielges
Keyword(s):  
Infrared Spectroscopy ◽  
Ir Spectroscopy ◽  
Protein Dynamics ◽  
Two Dimensional ◽  
Spatial And Temporal Scales ◽  
2D Ir ◽  
Functional Dynamics ◽  
Powerful Approach ◽  
Two Dimensional Infrared Spectroscopy

Proteins function as ensembles of interconverting structures. The motions span from picosecond bond rotations to millisecond and longer subunit displacements. Characterization of functional dynamics on all spatial and temporal scales remains challenging experimentally. Two-dimensional infrared spectroscopy (2D IR) is maturing as a powerful approach for investigating proteins and their dynamics. We outline the advantages of IR spectroscopy, describe 2D IR and the information it provides, and introduce vibrational groups for protein analysis. We highlight example studies that illustrate the power and versatility of 2D IR for characterizing protein dynamics and conclude with a brief discussion of the outlook for biomolecular 2D IR.

scholarly journals Does liquid–liquid phase separation drive peptide folding?

2021 ◽  
Author(s):  
Dean N. Edun ◽  
Meredith R. Flanagan ◽  
Arnaldo L. Serrano
Keyword(s):  
Infrared Spectroscopy ◽  
Phase Separation ◽  
Liquid Phase ◽  
Model Membrane ◽  
Peptide Folding ◽  
Two Dimensional ◽  
Intrinsically Disordered ◽  
Intrinsically Disordered Peptide ◽  
Two Dimensional Infrared Spectroscopy ◽  
Liquid Liquid Phase Separation

Two-dimensional infrared spectroscopy reveals folding of an intrinsically disordered peptide when sequestered into a model “membrane-less” organelle.

scholarly journals Understanding the structure and dynamics of hydrogenases by ultrafast and two-dimensional infrared spectroscopy

2019 ◽  
Vol 10 (39) ◽  
pp. 8981-8989 ◽  
Author(s):  
Marius Horch ◽  
Janna Schoknecht ◽  
Solomon L. D. Wrathall ◽  
Gregory M. Greetham ◽  
Oliver Lenz ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Two Dimensional ◽  
Proof Of Concept ◽  
Concept Study ◽  
Structure And Dynamics ◽  
Two Dimensional Infrared Spectroscopy

A proof-of-concept study on a catalytic [NiFe] intermediate reveals structural and dynamical details of hydrogenases by ultrafast and two-dimensional infrared spectroscopies.

scholarly journals Two-Dimensional Infrared Spectroscopy of Antiparallel β-Sheet Secondary Structure

2004 ◽  
Vol 126 (25) ◽  
pp. 7981-7990 ◽  
Author(s):  
Nurettin Demirdöven ◽  
Christopher M. Cheatum ◽  
Hoi Sung Chung ◽  
Munira Khalil ◽  
Jasper Knoester ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Secondary Structure ◽  
Two Dimensional ◽  
Two Dimensional Infrared Spectroscopy ◽  
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