The influence of photodynamic therapy parameters on the inactivation of Candida spp: in vitro and in vivo studies

Laser Physics ◽  
2014 ◽  
Vol 24 (4) ◽  
pp. 045601 ◽  
Author(s):  
F Alves ◽  
E G Mima ◽  
L N Dovigo ◽  
V S Bagnato ◽  
J H Jorge ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Studies ◽  
Candida Spp

scholarly journals Antimicrobial photodynamic therapy with fulleropyrrolidine: photoinactivation mechanism of Staphylococcus aureus, in vitro and in vivo studies

2015 ◽  
Vol 99 (9) ◽  
pp. 4031-4043 ◽  
Author(s):  
Mariusz Grinholc ◽  
Joanna Nakonieczna ◽  
Grzegorz Fila ◽  
Aleksandra Taraszkiewicz ◽  
Anna Kawiak ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
In Vivo Studies ◽  
Antimicrobial Photodynamic Therapy

scholarly journals Sonodynamic and sono-photodynamic therapy in oncology

2019 ◽  
Vol 8 (2) ◽  
pp. 31-46
Author(s):  
D. A. Tzerkovsky ◽  
E. L. Protopovich ◽  
D. S. Stupak
Keyword(s):  
Photodynamic Therapy ◽  
Aminolevulinic Acid ◽  
Pulse Frequency ◽  
Antitumor Efficacy ◽  
In Vivo Studies ◽  
Malignant Neoplasms ◽  
Serious Adverse Events ◽  
Treatment Methods

In the present publication, authors have analyzed the results of using sonodynamic and sono-photodynamic therapy with photosensitizing agents of various classes (hematoporphyrin, 5-aminolevulinic acid, chlorin derivatives, etc.) in experimental oncology. In a number of in vitro and in vivo studies, the high antitumor efficacy of the above treatment methods has been proven. Ultrasonic treatment with a pulse frequency of 1–3 MHz and an intensity of 0.7 to 5 W/cm2 , independently and in combination with photo-irradiation of experimental tumors, can significantly improve the cytotoxic properties of photosensitizers. This became the basisfor testing the methodsin patients with malignant neoplasms of various localizations. Scientists fromSouth-East Asia presented the preliminary results of the use of sonodynamic and sono-photodynamic therapy with photosensitizers in the treatment of malignant pathology of the mammary gland, stomach, esophagus, prostate, lung and brain. Analysis of the obtained data indicates the absence of serious adverse events and an increase in the antitumor efficacy of treatment, which included these treatment methods with chlorin-type photosensitizers. 

scholarly journals Porphyrin synthesis from ALA derivatives for photodynamic therapy. In vitro and in vivo studies

2004 ◽  
Vol 90 (8) ◽  
pp. 1660-1665 ◽  
Author(s):  
C Perotti ◽  
H Fukuda ◽  
G DiVenosa ◽  
A J MacRobert ◽  
A Batlle ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Studies ◽  
Porphyrin Synthesis

scholarly journals Liposomal Lapatinib in Combination with Low-Dose Photodynamic Therapy for the Treatment of Glioma

2019 ◽  
Vol 8 (12) ◽  
pp. 2214 ◽  
Author(s):  
Carl Fisher ◽  
Girgis Obaid ◽  
Carolyn Niu ◽  
Warren Foltz ◽  
Alyssa Goldstein ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Lines ◽  
Malignant Gliomas ◽  
Protoporphyrin Ix ◽  
Growth Factor Receptor ◽  
In Vivo Studies ◽  
Epidermal Growth

Background: Malignant gliomas are highly invasive and extremely difficult to treat tumours with poor prognosis and outcomes. Photodynamic therapy (PDT), mediated by Gleolan®, has been studied previously with partial success in treating these tumours and extending lifetime. We aim to determine whether combining PDT using ALA-protoporphyrin IX (PpIX) with a liposomal formulation of the clinical epidermal growth factor receptor (EGFR) inhibitor, lapatinib, would increase the anti-tumour PDT efficacy. Methods: Lapatinib was given in vitro and in vivo 24 h prior to PDT and for 3–5 days following PDT to elicit whether the combination provided any benefits to PDT therapy. Live-cell imaging, in vitro PDT, and in vivo studies were performed to elucidate the effect lapatinib had on PDT for a variety of glioma cell lines and as well as GSC-30 neurospheres in vivo. Results: PDT combined with lapatinib led to a significant increase in PpIX accumulation, and reductions in the LD50 of PpIX mediated PDT in two EGFR-driven cell lines, U87 and U87vIII, tested (p < 0.05). PDT + lapatinib elicited stronger MRI-quantified glioma responses following PDT for two human glioma-derived tumours (U87 and GSC-30) in vivo (p < 0.05). Furthermore, PDT leads to enhanced survival in rats following treatment with lapatinib compared to lapatinib alone and PDT alone (p < 0.05). Conclusions: As lapatinib is approved for other oncological indications, a realization of its potential combination with PDT and in fluorescence-guided resection could be readily tested clinically. Furthermore, as its use would only be in acute settings, long-term resistance should not pose an issue as compared to its use as monotherapy.

scholarly journals Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ballav M. Borah ◽  
Joseph Cacaccio ◽  
Farukh A. Durrani ◽  
Wiam Bshara ◽  
Steven G. Turowski ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Brain Tumor ◽  
Synergetic Effect ◽  
In Vivo Studies ◽  
Treatment Modalities ◽  
Type I ◽  
Sonodynamic Therapy ◽  
Biological Substrates

AbstractThis article presents the construction of a multimodality platform that can be used for efficient destruction of brain tumor by a combination of photodynamic and sonodynamic therapy. For in vivo studies, U87 patient-derived xenograft tumors were implanted subcutaneously in SCID mice. For the first time, it has been shown that the cell-death mechanism by both treatment modalities follows two different pathways. For example, exposing the U87 cells after 24 h incubation with HPPH [3-(1′-hexyloxy)ethyl-3-devinyl-pyropheophorbide-a) by ultrasound participate in an electron-transfer process with the surrounding biological substrates to form radicals and radical ions (Type I reaction); whereas in photodynamic therapy, the tumor destruction is mainly caused by highly reactive singlet oxygen (Type II reaction). The combination of photodynamic therapy and sonodynamic therapy both in vitro and in vivo have shown an improved cell kill/tumor response, that could be attributed to an additive and/or synergetic effect(s). Our results also indicate that the delivery of the HPPH to tumors can further be enhanced by using cationic polyacrylamide nanoparticles as a delivery vehicle. Exposing the nano-formulation with ultrasound also triggered the release of photosensitizer. The combination of photodynamic therapy and sonodynamic therapy strongly affects tumor vasculature as determined by dynamic contrast enhanced imaging using HSA-Gd(III)DTPA.

scholarly journals Methylene Blue-Based Nano and Microparticles: Fabrication and Applications in Photodynamic Therapy

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3955
Author(s):  
Dong-Jin Lim
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Textile Industry ◽  
In Vivo Studies ◽  
Future Perspective ◽  
Pharmacological Properties ◽  
Hydrophilic Nature ◽  
Recent Trends

Methylene blue (MB) has been used in the textile industry since it was first extracted by the German chemist Heinrich Caro. Its pharmacological properties have also been applied toward the treatment of certain diseases such as methemoglobinemia, ifosfamide-induced encephalopathy, and thyroid conditions requiring surgery. Recently, the utilization of MB as a safe photosensitizer in photodynamic therapy (PDT) has received attention. Recent findings demonstrate that photoactivated MB exhibits not only anticancer activity but also antibacterial activity both in vitro and in vivo. However, due to the hydrophilic nature of MB, it is difficult to create MB-embedded nano- or microparticles capable of increasing the clinical efficacy of the PDT. This review aims to summarize fabrication techniques for MB-embedded nano and microparticles and to provide both in vitro and in vivo examples of MB-mediated PDT, thereby offering a future perspective on improving this promising clinical treatment modality. We also address examples of MB-mediated PDT in both cancer and infection treatments. Both in-vitro and in-vivo studies are summarized here to document recent trends in utilizing MB as an effective photosensitizer in PDT. Lastly, we discuss how developing efficient MB-carrying nano- and microparticle platforms would be able to increase the benefits of PDT.

A Diode Laser-Based Singlet Oxygen Monitor for Photodynamic Therapy; in- vitro and in- vivo Studies

2006 ◽  
Author(s):  
Seonkyung Lee ◽  
Danthu H. Vu ◽  
Michael F. Hinds ◽  
Steven J. Davis ◽  
Tayyaba Hasan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Diode Laser ◽  
In Vivo Studies ◽  
Oxygen Monitor
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