Effect of chitosan as adsorben to bacto agar quality from Gelidium sp.

It was showed that gums induced by Fusarium oxysporum f.sp. tulipae in tulip bulbs applied to mineral Czapek-Dox Broth with Bacto Agar (CzDA) medium, containing sucrose substantially stimulated abundant growth of mycelium and sporulation of the pathogen. Addition of arabinose, xylose and their mixture, sugars occurring in tulip gum polysaccharide, to CzDA medium caused that mycelium was very sparse and sporulation was only slightly stimulated by arabinose. Mycelium growth on mineral CzDA medium without sucrose, was poor and sparse but addition of gum to the medium caused formation of abundant mycelium and increased sporulation of the pathogen. It is possible that polysaccharide of tulip gum may act mainly as elicitor and partially as substrate in regulation of mycelium growth and sporulation of Fusarium oxysporum f.sp. tulipae.

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PENGARUH BAP (BENZIL ADENIN PURIN) DAN NAA (NAPHTHALEN ACETIC ACID) TERHADAP EKSPLAN TANAMAN TURI (Sesbania grandiflora) DALAM MEDIA MULTIPLIKASI In Vitro

Pastura ◽

10.24843/pastura.2015.v05.i01.p13 ◽

2017 ◽

Vol 5 (1) ◽

pp. 35

Author(s):

Mardhiyetti Mardhiyetti ◽

Zulfadli Syarif ◽

Novirman Jamarun ◽

Irfan Suliansyah

Keyword(s):

Acetic Acid ◽

Sesbania Grandiflora ◽

Murashige Skoog ◽

Bacto Agar

Turi merupakan leguminosa pohon. Perbanyakan turi secara vegetatif sulit dilakukan karena kemampuan turi untuk tumbuh kembali setelah dilakukan pemotongan sangat rendah. Penelitian dalam upaya perbanyakan turi melalui metode multiplikasi tanaman turi secara in vitro telah dilakukan di Laboratorium Kultur Jaringan. Eksplan yang digunakan adalah kotiledon, daun dan hipokotil steril tanaman turi. Media dasar yang digunakan adalah MS (Murashige skoog) yang diperkaya dengan vitamin. Sebagai sumber energi digunakan sukrosa sebanyak 30 g/l dan bacto agar 8g/l. Penelitian ini terdiri dari perlakuan BAP sebagai salah satu jenis sitokinin) secara tunggal yaitu MS + BAP 1 mg/l dan MS + BAP 2 mg/l, dan kombinas BAP dengan NAA (sebagai salah satu jenis auksin) yaitu MS + BAP 1mg/l + NAA 0,08 mg/l, MS + BAP 2 mg/l + NAA 0,08 mg/l. Hasil penelitian menunjukkan bahwa kombinasi auksin dan sitokinin mampu memperbanyak shootled turi.Kata kunci: turi, ksplan, Benzil Adenin Purin, Naphthalen acetic acid, in vitro

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Controlling vitrification in Larixdecidua via culture media manipulation

Canadian Journal of Forest Research ◽

10.1139/x89-205 ◽

1989 ◽

Vol 19 (10) ◽

pp. 1334-1337 ◽

Cited By ~ 4

Author(s):

J. McLaughlin ◽

D. F. Karnosky

Keyword(s):

Mortality Rate ◽

Total Nitrogen ◽

Normal Development ◽

Culture Media ◽

Adventitious Bud ◽

Adventitious Buds ◽

Reversion Frequency ◽

The Media ◽

Media Manipulation ◽

Bacto Agar

Vitrification rates obtained from our inverted embryo system were significantly decreased by lowering the cytokinin concentrations in Brown and Lawrence medium containing 10 mM L-glutamine from 10 to 1 mg/L, or by replacing 10 mM L-glutamine with equimolar concentrations of Ca(NO3)2 or by adding 1 g/L Gelrite to the normal 10 g/L Difco–Bacto agar in the culture media. In all treatments, decreased vitrification was accompanied with decreased adventitious bud production. With reduced N6-benzylaminopurine, vitrification decreased from 77 to 29%, but bud production decreased from 61 to 17 buds per explant and mortality increased from 3 to 33%. Incorporation of Ca(NO3)2 into the media decreased vitrification from 87 to 21%, but the number of adventitious buds per embryo decreased from 75 to 42. Vitreous shoots were reverted to normal development with an 81% reversion frequency and a 6% mortality rate by culturing these shoots on Gresshof and Doy medium with one-half total nitrogen. Elongation of previously vitreous shoots was best when these shoots were cultured on Gresshof and Doy medium + 0.5 mg/L N6-benzylaminopurine for 2 weeks, followed by subculture on Gresshof of and Doy medium + 10 g/L charcoal.

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