scholarly journals Mangifera foetida L. (Macang) Source of Potent Antiviral Activity of Against Dengue Virus Serotype 2 (Anti DENV2)

2021 ◽  
Vol 2049 (1) ◽  
pp. 012018
Author(s):  
Fitmawati ◽  
Maya Safitri ◽  
S.N. Kholifah ◽  
Emrizal ◽  
Rodesia Mustika Roza
Keyword(s):  
Dengue Virus ◽  
Vero Cells ◽  
Stem Bark ◽  
Selectivity Index ◽  
Assay Method ◽  
Bark Extract ◽  
Standard Curve ◽  
Virus Serotype ◽  
Stem Bark Extract

Abstract The new discovery about the potential of Mangifera foetida L. as an antiviral will help conservation efforts in nature while maintaining and increasing its biodiversity value. This study aims to characterize the in vitro potential of three varieties of M. foetida L. against the dengue virus. Dengue virus is infected in Vero cells, viral replication was measured using the Viral ToxGlo Assay method. The selectivity ability of Mangifera foetida L. stem bark extract to inhibit the dengue virus was seen from the Selectivity Index (SI) value. The standard curve between the concentration of the compound (μg/mL) and % cell viability was analyzed by linear regression using Microsoft Excel 2010 software. The results showed that the selectivity index (SI) value of M. foetida L stem bark extract of Limus, Manis and Batu varieties were 7.58, 6.82 and 16.43, respectively. It was concluded that the extract of Macang stem bark of the Limus, Manis and Batu varieties had the potential to be used as an antiviral for dengue.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF KNEMA ATTENUATA (HOOK. F. AND THOMSON) WARB ETHANOLIC STEM BARK EXTRACT IN ALBINO WISTAR RATS

2019 ◽  
pp. 330-334
Author(s):  
SUPRIYA RAJA H
Keyword(s):  
Stem Bark ◽  
Inflammatory Activity ◽  
Assay Method ◽  
Bark Extract ◽  
Raw 264.7 ◽  
Myeloperoxidase Activity ◽  
Stem Bark Extract ◽  
Anti Inflammatory

Objective: Knema attenuata (Myristicaceae), popularly known as “wild nutmeg,” is an endemic tree species from Western Ghats, which has been used in folk medicine. Conventionally, the stem bark of K. attenuata is used for treating inflammatory conditions without any scientific information available for the same. The present study was undertaken to evaluate the anti-inflammatory activity of the ethanolic stem bark extract (ESBE) of K. attenuata using in vivo and in vitro screening models. Methods: The ethanolic extract of stem bark was prepared by soxhlation, and its cytotoxicity in RAW 264.7 cell line was assessed using MTT assay method. In vivo anti-inflammatory effect of extract was estimated in rats using carrageenan-induced paw edema model and cotton pellet-induced granuloma model. The in vitro anti-inflammatory activity of the extract was evaluated by cyclooxygenase and lipoxygenase inhibition assay, estimation of myeloperoxidase activity, and determination of cellular nitrite levels in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Results: Toxic symptoms were not observed for the ESBE. The extract demonstrated significant anti-inflammatory activity in both in vivo and in vitro models. The anti-inflammatory action exhibited by the extract was a result of the inhibition of leukocyte migration and nitric oxide pathway and partially by inhibition of mediators such as prostaglandins and leukotrienes. Conclusion: Findings from the study provide the evidence for the popular use of stem bark extract of K. attenuata as a potential anti-inflammatory agent.

scholarly journals Spondias purpurea L. Stem Bark Extract: Antioxidant and in vitro Photoprotective Activities

2021 ◽  
Author(s):  
Francisco Alessandro Rodrigues ◽  
Priscylla Giffony ◽  
Sarah dos Santos ◽  
Jhonyson Guedes ◽  
Maria Elenir Ribeiro ◽  
...  
Keyword(s):  
Active Ingredient ◽  
Antioxidant Activities ◽  
Stem Bark ◽  
Ultra Performance Liquid Chromatography ◽  
Bark Extract ◽  
Protection Factor ◽  
Phenolic Constituents ◽  
Stem Bark Extract ◽  
Spondias Purpurea

Plant phenolic extracts are widely recognized as an important source of natural antioxidant substances and potential compounds for cosmetic formulations. This study aimed to evaluate the chemical profile, photoprotective and antioxidant activities of stem bark extract of Spondias purpurea L. (ciriguela) and its application in photoprotective formulations. Thirty phenolic constituents were annotated by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry in mode negative (UPLC‑QTOF-MS2). The stem bark extract antioxidant and chelation potential, expressed in half maximal inhibitory concentration (IC50), showed 6.25 and 352.22 μg mL−1, respectively. The phenolic extract was used as an active ingredient in six sunscreen formulations, with concentrations ranging from 0.2 to 10%. The ultraviolet (UV) protection properties of the formulations were evaluated by sun protection factor (SPF) values obtained in 0.2 mg mL−1 (0.495 to 2.27) and 2.0 mg mL−1 (2.29 to 15.87). The SPF value for the extract (14.37 and 26.16) was high, but there was a reduction in the base formulation. However, these results suggested that the bioactive extracted of stem bark of Spondias purpurea L. has interesting potential to reduce the damage caused by UV radiation and may be utilized as an active ingredient in a sunscreen formulation.

Bioactive Compounds and in vitro Antimicrobial Activities of Ethanol Stem Bark Extract of Trilepisium madagascariense DC.

2018 ◽  
Vol 14 (7) ◽  
pp. 901-912 ◽  
Author(s):  
Olufunmiso Olusola Olajuyigbe ◽  
Tolulope Esther Onibudo ◽  
Roger Murugas Coopoosamy ◽  
Anofi Omotayo Tom Ashafa ◽  
Anthony Jide Afolayan
Keyword(s):  
Bioactive Compounds ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Bark Extract ◽  
Stem Bark Extract

Lack of in vitro antihyaluronidase activity of methanolic leaf extract of Indigofera tinctoria L and methanolic stem bark extract of Stereospermum suaveolens DC

2015 ◽  
Vol 6 (1) ◽  
pp. 40
Author(s):  
WanigasekaraDaya Ratnasooriya ◽  
ChathurangaBharathee Ranaweera ◽  
WalimuniPrabhashini Abeysekara ◽  
Ranjith Pathirana
Keyword(s):  
Leaf Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Indigofera Tinctoria

scholarly journals Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2

2020 ◽  
Author(s):  
Teguh H. Sucipto ◽  
Fahimah Martak
Keyword(s):  
Dengue Virus ◽  
Inhibitory Concentration ◽  
Platinum Complexes ◽  
Hemorrhagic Fever ◽  
Fold Increase ◽  
Vero Cells ◽  
Inhibitory Effect ◽  
Selectivity Index ◽  
Antiviral Compound ◽  
Virus Serotype

Dengue fever and dengue hemorrhagic fever are transmitted to humans by the Aedes aegypti and Aedes albopictus mosquitoes, with an observed 30-fold increase in global incidence the last 50 years. Despite the tremendous efforts invested anti-dengue virus research, no clinically approved vaccine or antiviral chemotherapeutics are available for humans, and disease treatment is limited to supportive care. Over the years there has been a continuous interest in the chemistry of metal complexes with biological activity, including platinum complexes with antitumor activity and silver complexes with antimicrobial action. Aim of the project was to investigate [Cu(2,4,5-triphenyl-1H- imidazole)2 (H2O)2].Cl2 as antiviral compound that was further tested for inhibitory effect on the replication of dengue virus type 2 (DENV-2) in Vero cell. DENV-2 were infected in Vero cells and replication of virus was measured by Viral ToxGlo with selectivity index value (SI) and determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for com- pound. The standard curve between concentration of compound (μg/mL) and %viability of cells was analyzed by logarithmic cor- relation regression with regression equation. For infection rates, t-test was used to exam- ine the statistical significances among the concentrations of compound. P<0.05 was considered to be significant. The maximum inhibitory concentration (IC50) of [Cu(2,4,5- triphenyl-1H-imidazole)2 (H2O)2].Cl2 against DENV-2 was 98.62 μg/mL. The cytotoxic concentration (CC50) of compound against Vero cells was 300.36μg/mL. The SI values for [Cu(2,4,5-triphenyl-1H-imidazole)2 (H2O)2].Cl2 1.86. Based on selectivity index values, [Cu(2,4,5-triphenyl-1H-imidazole)2 (H2O)2].Cl2 can inhibit the growth of DENV- 2 and has low toxicity values for Vero cells.

In vitro anti-hyperglycemia properties of the aqueous stem bark extract from Strychnos henningsii (GILG)

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
SO Oyedemi ◽  
T Koekemoer ◽  
G Bradley ◽  
M van de Venter ◽  
AJ Afolayan
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract

Antimicrobial triterpenes from the stem bark of Crossopteryx febrifuga

2015 ◽  
Vol 70 (7-8) ◽  
pp. 169-173 ◽  
Author(s):  
Jean Rodolphe Chouna ◽  
Jean-de-Dieu Tamokou ◽  
Pépin Nkeng-Efouet-Alango ◽  
Bruno Ndjakou Lenta ◽  
Norbert Sewald
Keyword(s):  
Candida Parapsilosis ◽  
Enterobacter Aerogenes ◽  
Stem Bark ◽  
Bark Extract ◽  
Dioic Acid ◽  
Antibacterial And Antifungal Activities ◽  
Phytochemical Investigation ◽  
Stem Bark Extract ◽  
Antibacterial And Antifungal

Abstract Phytochemical investigation of the stem bark extract of Crossopteryx febrifuga resulted in the isolation of epimeric mixtures of 3β-urs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-urs-12,20(30)-diene-27,28-dioic acid (1), as well as: 3β-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid (2), together with some known compounds such as the monoglyceride of palmitic acid, as well as β-sitosterol and its glucoside. The structures of the isolated compounds were determined by application of spectroscopic methods. The MeOH extract and compounds 1 and 2 were examined for antimicrobial activity in in vitro assays against bacteria (Enterobacter aerogenes ATCC13048, Escherichia coli ATCC8739, Klebsiella pneumoniae ATCC11296, Staphylococcus aureus) and fungi (Candida parapsilosis, Candida albicans ATCC 9002 and Cryptococcus neoformans IP 90526). The tested samples showed selective activities. The antibacterial and antifungal activities of compound 2 (MIC=8–64 μg/mL) were in some cases equal to or even higher than those of the respective reference drugs chloramphenicol (MIC=16– 64 μg/mL) and nystatin (MIC=128–256 μg/mL).

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