scholarly journals Preliminary plant design of Escherichia coli BPPTCC-EgRK2 cell culture for recombinant cellulase production using Oil Palm Empty Fruit Bunch (OPEFB) as substrate

Author(s):  
E A Surya ◽  
S F Rahman ◽  
S Zulamraini ◽  
M Gozan
BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 291-307
Author(s):  
Mahdi Shahriarinour ◽  
Mohd Noor Abdul Wahab ◽  
Shuhaimi Mustafa ◽  
Rosfarizan Mohamad ◽  
Arbakariya B. Ariff

The possibility of using treated oil palm empty fruit bunch (OPEFB) fibres as substrate for cellulase production by Aspergillus terreus was studied using shaking flask fermentation. The effect of different chemical pretreatments, i.e. formic acid, acetic acid, propylamine, phosphoric acid, and n-butylamine, on the suitability of OPEFB fibres as fermentation substrate was investigated. The findings revealed that pretreatment with these chemicals significantly (P<0.05) increased the cellulose and reduced the lignin contents prior to enzymatic hydrolysis. However, fermentation using OPEFB fibres pretreated with phosphoric acid gave the highest cellulase production, which was related to high cellulose content. Further improvement in cellulase production was obtained when the chemically pretreated OPEFB fibres were subsequently treated hydrothermally (autoclaved at 160oC for 10 min) and then biologically (using effective microorganisms). The final activity of the three main components of cellulase (FPase, CMCase, and β-glucosidase) obtained in fermentation by A. terreus using optimally treated OPEFB fibres was (0.77 U mL−1, 8.5 U mL-1, and 6.1 U mL-1), respectively. The production of all these three major components of cellulase using pretreated OPEFB fibres (i.e. chemical, hydrothermal, and biological) were about three times higher than those obtained from fermentation using untreated OPEFB fibres.


BioResources ◽  
2012 ◽  
Vol 8 (1) ◽  
Author(s):  
Nor Amira Farhana Harun ◽  
Azhari Samsu Baharuddin ◽  
Mohd Huzairi Mohd Zainudin ◽  
Ezyana Kamal Bahrin ◽  
M. Nazli Naim ◽  
...  

2017 ◽  
Vol 9 (1) ◽  
pp. 17-28
Author(s):  
I Dewa Gede Putra Prabawa ◽  
Nurmilatina Nurmilatina

The objective of this research was to analyse organic fertilizer pellets from water hyacinth and oil palm empty fruit bunch (OPEFB) that have not been widely used in South Kalimantan. The variable of the research was the ratio of water hyacinth and OPEFB: 25%:75%, 50%:50%, and 75%:25%. The fertilizer was produced by heating the materials at 350oC, and then composting the materials using effective microorgnism 4 (EM4), and then mold into pellet shape. The results showed that the drying, heating, and composting process could increase nitrogen and phosphor content. Nitrogen in water hyacinth increased from 0.06% to 2.18%, while its increased from 0.18% to 2.49% in OPEFB. Phosphor in water hyacinth increased from 3.42% to 6.89%, while its increased from 1.95% to 4.70% in OPEFB. The quality of organic fertilizer pellet was evaluated according to Permentan No.70-2011. The result showed that the content of C-organic, macro nutrients, micro nutrients, heavy metals, Escherichia coli, Salmonella sp., moisture content, pH, and residue met the requirements. The organic fertilizer contained the highest nutrient made from 50 % water hyacinth and 50% OPEFB.Keywords: organic fertilizer pellets, oil palm empty fruit bunch, water hyacinth


1997 ◽  
Vol 62 (1-2) ◽  
pp. 1-9 ◽  
Author(s):  
M.S. Umikalsom ◽  
A.B. Ariff ◽  
H.S. Zulkifli ◽  
C.C. Tong ◽  
M.A. Hassan ◽  
...  

2014 ◽  
Vol 61 (4) ◽  
pp. 426-431 ◽  
Author(s):  
Qingxin Li ◽  
Wei Ting Ng ◽  
Sze Min Puah ◽  
Ravindran Vijay Bhaskar ◽  
Loon Siong Soh ◽  
...  

2017 ◽  
Vol 28 (Suppl. 1) ◽  
pp. 171-184 ◽  
Author(s):  
Barbara Ngikoh ◽  
◽  
Noor Adila Abdul Karim ◽  
Jamaliah Jahim ◽  
Farah Diba Abu Bakar ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document