Phytase production and phytic acid reduction in rapeseed meal by Aspergillus niger during solid state fermentation

2001 ◽  
Vol 34 (8) ◽  
pp. 715-720 ◽  
Author(s):  
A.I El-Batal ◽  
H Abdel Karem
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Phytic Acid ◽  
Solid State Fermentation ◽  
Rapeseed Meal ◽  
Phytase Production ◽  
Acid Reduction

scholarly journals Enhanced phytase production by Aspergillus niger mutants in solid state fermentation

2021 ◽  
Author(s):  
Shahzad Mahmood ◽  
◽  
Memuna G. Shahid ◽  
Muhammad Nadeem ◽  
Rubina Nelofer ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Research Work ◽  
Wild Strain ◽  
Chemical Mutagenesis ◽  
Poultry Feed ◽  
Control Group ◽  
Phytase Production

The present research work was conducted to improve the phytase production by genetic alteration of Aspergillus niger with induced mutagenesis using solid state fermentation. Strain improvement was carried out in the presence of ultra violet (UV) irradiation and ethylmethane sulphonate (EMS) [0.5% v/v] treatments for various time intervals. We reported an improved strain of Aspergillus niger designated as UV-3 mutant producing a zone of hydrolysis of about 40 mm, in comparison to wild strain (26 mm). The highest enzyme activity was found to be 547.64 IU/g for UV-3 mutant followed by EMS-4 mutant (492.23 IU/g)compared to wild strain which showed 406.45 IU/g of enzyme activity. There was 1.35 fold increase in phytase production after mutation studies of Aspergillus niger. Phytase was applied as poultry feed additive and given to broiler chickens for 5 weeks. The results exhibited that there was increase in body weight gain (BWG) of chicks for experimental group (2028 g) in comparison to control group (1903 g). Thus, physical and chemical mutagenesis was proved as an effective technique for the improvement of strain and ultimately for enhanced and economical phytase production for different industrial applications.

scholarly journals Phytase Production byAspergillus nigerCFR 335 andAspergillus ficuumSGA 01 through Submerged and Solid-State Fermentation

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Gunashree B. Shivanna ◽  
Govindarajulu Venkateswaran
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Level ◽  
Solid Substrate ◽  
Aspergillus Ficuum ◽  
Phytase Production ◽  
Potato Dextrose Broth ◽  
Substrate Medium

Fermentation is one of the industrially important processes for the development of microbial metabolites that has immense applications in various fields. This has prompted to employ fermentation as a major technique in the production of phytase from microbial source. In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase fromAspergillus nigerCFR 335 andAspergillus ficuumSGA 01. It was found that both the fungi were capable of producing maximum phytase on 5th day of incubation in both submerged and solid-state fermentation media.Aspergillus nigerCFR 335 andA. ficuumproduced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat bran solid substrate medium. Enhancement in the enzyme level (76 and 50.7 U/gds) was found when grown in a combined solid substrate medium comprising wheat bran, rice bran, and groundnut cake in the ratio of 2 : 1 : 1. A maximum of 9.6 and 8.2 U/mL of enzyme activity was observed in SmF byA. nigerCFR 335 andA.ficuum, respectively, when grown in potato dextrose broth.

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