Effect of green bean sprout extract on in vitro shoot multiplication of taro Colocasia esculenta L. var. antiquorum

Effect of Thidiazuron and Casein Hydrolysate on In Vitro Shoot Multiplication of Satoimo (Colocasia esculenta (L.) Schott var antiquorum)ABTRACTSatoimo (Colocasia esculenta (L.) Schott var antiquorum) is an alternative substitute of rice which has a big potential to be developed in Indonesia as an export commodity to Japan. Satoimo production needs to be increased to meet the demands for of the plant seeds. Plant propagation can be done using optimal media to stimulate the formation of shoots through, amongst others, the addition of thidiazuron (TDZ) and casein hydrolysate into the culture medium. This study aimed to determine the optimal concentration of TDZ and casein hydrolysate for in vitro multiplication of satoimo shoots. This research used RAL method with 2 factorials, namely the addition of TDZ at 0; 0.2; 0.6 mg/L concentrations, and of casein hydrolysate at 0; 150; 300; 450 mg/L concentrations. The results showed that the use of 0.6 mg/L TDZ and 150 mg/L casein hydrolysate resulted in the highest number of shoots, with the shoot average number of 6.9 per explant.Keywords: Casein hydrolysate, optimal medium, Satoimo, shoot multiplication, TDZ ABTRAKSatoimo (Colocasia esculenta (L.) Schott var antiquorum) merupakan salah satu bahan alternatif pengganti beras yang memiliki peluang besar untuk dikembangkan di Indonesia, salah satunya sebagai komoditas ekspor ke negara Jepang. Produksi satoimo perlu ditingkatkan untuk memenuhi kebutuhan bibit tanaman tersebut. Perbanyakan tanaman dapat dilakukan menggunakan media yang optimal untuk merangsang pembentukan tunas, salah satunya dengan penambahan thidiazuron (TDZ) dan hidrolisat kasein pada media tanam. Penelitian ini bertujuan untuk mengetahui konsentrasi TDZ dan hidrolisat kasein yang optimal untuk perbanyakan tunas satoimo secara in vitro. Penelitian ini menggunakan metode RAL dengan 2 faktorial yaitu konsentrasi TDZ yang terdiri dari 0; 0,2; 0,6 mg/L dan konsentrasi hidrolisat kasein yang terdiri dari 0; 150; 300; 450 mg/L. Hasil penelitian menunjukkan bahwa pemberian TDZ 0,6 mg/L dan hidrolisat kasein 150 mg/L menghasilkan jumlah tunas tertinggi, dengan rata-rata tunas yang terbentuk 6,9 per eksplan.Kata kunci: Hidrolisat kasein, multiplikasi tunas, optimasi media, Satoimo, TDZ

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MULTIPLIKASI TUNAS DAN INDUKSI UMBI MIKRO SATOIMO (Colocasia esculenta (L.) Schott) PADA BEBERAPA KONSENTRASI SUKROSA DAN BENZILAMINOPURIN

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v3i2.150 ◽

2016 ◽

Vol 3 (2) ◽

pp. 81

Author(s):

Delvi Maretta ◽

Dwi Pangesti Handayani ◽

Henti Rosdayanti ◽

Armelia Tanjung

Keyword(s):

In Vitro Culture ◽

Sucrose Concentration ◽

Shoot Multiplication ◽

Colocasia Esculenta ◽

Mass Cultivation ◽

In Vitro Technique ◽

Two Factors ◽

Microtuber Induction ◽

Single Effect

Taro or Satoimo (Colocasia esculenta (L) Schott var antiquorum) is an alternative of non-rice food. To support saitomo mass cultivation in several regions in Indonesia, shoot multiplication and induction of satoimo microtuber through in vitro technique is amongst the stage to be undertaken. The aims of this study were to determine the effect of BAP (benzylaminopurine) and sucrose for shoot multiplication and microtuber induction of in vitro culture of satoimo. The experiment was arranged in two factors: BAP (0, 1, 2 and 3 mg/L) and sucrose (30, 60, 90 and 120 g/L). The result showed that the single effect of BAP or sucrose and interaction of both significantly increased the number of shoots. The effect of 2 mg/L BAP was more homogeneous than that of 1 and 3 mg/L BAP. Sucrose with the concentration of 30 g/L was the best concentration for shoot multiplication. The highest number of microtuber was achieved with 2 mg/L BAP + 30 g/L sucrose treatments, but tended to decrease due to increasing sucrose concentration. In 2 and 3 mg/L BAP treatments, the number of microtuber increased along with the increasing sucrose concentration.Keywords: satoimo, in vitro shoot, microtuber, benzylaminopurine, sucrose ABSTRAKSatoimo (Colocasia esculenta (L) Schott var antiquorum) merupakan bahan pangan alternatif non-beras. Untuk mendukung produksi massal satoimo di beberapa wilayah di Indonesia, multiplikasi tunas dan induksi umbi mikro secara in vitro merupakan tahapan yang harus dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh BAP dan sukrosa terhadap multiplikasi tunas dan induksi umbi mikro satoimo dalam kultur in vitro. Perlakuan terdiri dari 4 taraf konsentrasi BAP (0, 1, 2 dan 3 mg/L) dan 4 taraf konsentrasi sukrosa (30, 60, 90 dan 120 g/L). Hasil penelitian menunjukkan bahwa BAP dan sukrosa secara tunggal serta interaksinya berpengaruh nyata terhadap multiplikasi tunas in vitro. Pengaruh konsentrasi BAP 2 mg/L lebih homogen dibandingkan perlakuan BAP 1 dan 3 mg/L. Sukrosa 30 g/L merupakan konsentrasi terbaik untuk multiplikasi tunas. Umbi mikro terbanyak terdapat pada perlakuan BAP 1 mg/L + sukrosa 30 g/L tetapi cenderung mengalami penurunan jika konsentrasi sukrosa dinaikkan pada konsentrasi BAP tetap. Pada perlakuan BAP 2 dan 3 mg/L jumlah umbi mikro yang terbentuk cenderung meningkat seiring dengan peningkatan konsentrasi sukrosa.Kata kunci: satoimo, tunas in vitro, umbi mikro, benzilaminopurin, sukrosa

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OPTIMASI MEDIUM PERBANYAKAN IN VITRO TUNAS TALAS KALIURANG (Colocasia esculenta L.) DIPLOID DAN TETRAPLOID

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v7i2.3944 ◽

2020 ◽

Vol 7 (2) ◽

pp. 171-181

Author(s):

Khalisa Aini Sinaga ◽

Dyah Retno Wulandari ◽

Diah Ratnadewi

Keyword(s):

Flow Cytometry ◽

Shoot Proliferation ◽

Shoot Multiplication ◽

Colocasia Esculenta ◽

Plant Materials ◽

Pests And Diseases ◽

Good Taste ◽

Rooting Medium ◽

Optimum Formulation

Optimation of In Vitro Shoot Proliferation Medium for Diploid and Tetraploid Kaliurang Taro (Colocasia esculenta L.) Taro cv. Kaliurang has a good taste and is tolerant to certain pests and diseases but its development is hampered by insufficient number of good quality plant materials. Quality improvement has been carried out through polyploidization. Shoot multiplication is an important step in micropropagation, which often needs specific formulation of culture medium. This study aimed to obtain an optimum formulation of in vitro shoot-inducing medium of taro cv. Kaliurang. Explants from one diploid and three tretraploid clones were subjected to six treatments of medium formulations with various concentrations of thiamine and adenine in BAP-containing MS media. Shoots were then rooted, followed by plantlet acclimatization. Ploidy level was measured using flow cytrometry. The rooting medium was ½ MS without growth hormones, whereas acclimatization medium was a mixture of sterile soil, husk, and cocopeat. The results showed that MS + 2 mg L-1 BAP + 4 mg L-1 thiamine + 2 mg L-1 adenine was the optimum medium with an average 3.45 shoots per explant. Plantlet acclimatization was successful with 99.1% survival. Flow cytometry measurement confirmed tetraploidy level of the regenerants from 3 tetraploid clones. Keywords: adenine, acclimatization, benzyl amino purine, shoot proliferation, thiamine ABSTRAK Talas Kaliurang memiliki rasa yang enak dan toleran terhadap hama dan penyakit tertentu, namun pengembangannya terkendala oleh ketercukupan benih bermutu. Upaya perbaikan mutu tanaman telah dilakukan sebelumnya melalui poliploidisasi. Perbanyakan tunas merupakan langkah penting, yang membutuhkan formula spesifik untuk media kultur. Penelitian ini bertujuan untuk mengoptimasi medium perbanyakan tunas in vitro dari satu klon diploid dan tiga klon tetraploid, yang dikulturkan pada enam konsentrasi tiamin dan adenin dalam media MS yang mengandung BAP. Tunas kemudian diinduksi akar, lalu diaklimatisasi, dan pengukuran tingkat ploidi menggunakan flow cytometry. Media pengakaran adalah ½ MS tanpa ZPT, media aklimatisasi adalah campuran tanah steril, sekam dan kokopit. Hasil penelitian ini menunjukkan bahwa medium MS + 2 mg L-1 BAP + 4 mg L-1 tiamin + 2 mg L-1 adenin merupakan medium optimum dengan rata-rata 3,45 tunas per-eksplan. Hasil aklimatisasi menunjukkan bahwa 99,1% tanaman dapat bertahan hidup. Analisis ploidi dengan flow cytometry menunjukkan bahwa tanaman hasil regenerasi tunas talas Kaliurang tetraploid memiliki tingkat ploidi yang stabil.

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RAPID IN VITRO PROPAGATION OF WESTERN GHATS CULTIVAR - COLOCASIA ESCULENTA FOR THE HIGH FREQUENCY OF PLANTLETS

Journal of Drug Discovery and Therapeutics ◽

10.32553/jddt.v7i2.447 ◽

2019 ◽

Vol 7 (2) ◽

Author(s):

Jyothi R ◽

Srinivasa Murthy K M ◽

Hossein . ◽

Veena .

Keyword(s):

Tissue Culture ◽

Wide Distribution ◽

Colocasia Esculenta ◽

Limiting Factor ◽

Promising Alternative ◽

Rapid Multiplication ◽

Medical Plant ◽

Total Fat ◽

Planting Materials

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

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Effect of additives on enhanced in vitro shoot multiplication and GC-MS analysis of Lawsonia inermis L.

Research on Crops ◽

10.31830/2348-7542.2018.0001.27 ◽

2018 ◽

Vol 19 (3) ◽

Keyword(s):

Shoot Multiplication ◽

Lawsonia Inermis ◽

Effect Of Additives ◽

Ms Analysis

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Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

Molecules ◽

10.3390/molecules26113229 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3229

Author(s):

Mat Yunus Najhah ◽

Hawa Z. E. Jaafar ◽

Jaafar Juju Nakasha ◽

Mansor Hakiman

Keyword(s):

Callus Induction ◽

Antioxidant Activities ◽

Shoot Multiplication ◽

Wild Plants ◽

Wild Plant ◽

Total Phenolic ◽

Nodal Segment ◽

Ms Medium ◽

In Vitro Plantlets

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

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Influence of Cytokinins in Combination with GA3on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis(L.) O. Kuntze)

The Scientific World JOURNAL ◽

10.1155/2014/943054 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Reza Azadi Gonbad ◽

Uma Rani Sinniah ◽

Maheran Abdul Aziz ◽

Rosfarizan Mohamad

Keyword(s):

Camellia Sinensis ◽

Woody Plants ◽

Clonal Propagation ◽

Shoot Multiplication ◽

Shoot Elongation ◽

Nodal Segments ◽

Nodal Segment ◽

Liquid Form ◽

Solid Media

The use ofin vitroculture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis(L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA3) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA3. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

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In vitro shoot multiplication using meta-Topolin and leaf-based regeneration of a withaferin A rich accession of Withania somnifera (L.) Dunal

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113872 ◽

2021 ◽

Vol 171 ◽

pp. 113872

Author(s):

Kuldeep Kaur ◽

Kulwinder Kaur ◽

Abhishek Bhandawat ◽

Pratap Kumar Pati

Keyword(s):

Withania Somnifera ◽

Shoot Multiplication ◽

Withaferin A

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Effects of Sucrose and Other Additives on In Vitro Growth and Development of Purple Coneflower (Echinacea purpurea L.)

Advances in Biology ◽

10.1155/2014/402309 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 3

Author(s):

Dahanayake Nilanthi ◽

Yue-Sheng Yang

Keyword(s):

Plant Growth ◽

Culture Medium ◽

Respiratory Infections ◽

Shoot Multiplication ◽

Echinacea Purpurea ◽

Naphthaleneacetic Acid ◽

Acid Plant ◽

Purple Coneflower ◽

Upper Respiratory

Echinacea purpurea (purple coneflower) is being used for the preparation of more than 240 extracts, salves, and tinctures to help cure diseases like rabies, cold, and upper respiratory infections. Hence, efforts were made to develop a culture medium for successful in vitro culturing of cornflower and to regenerate buds and induce roots to enable mass propagation of selected clones. Of the three levels of sucrose tested as a supplement to MS media (Murashige and Skoog’s medium, 1962) 3% showed better rooting of buds and appeared morphologically normal and identical as compared to those grown at higher and lower concentrations (2 and 4%). The additives hydrolyzed lactabumin (0.0, 100, 300, and 900 mgL−1), peptone (0.0, 100, 300, and 900 mgL−1), and yeast (0.0, 100, 300, and 900 mgL−1) to media containing 0.3 mgL−1 BA (6-benzyladenine) and 0.01 mgL−1 NAA (naphthaleneacetic acid-plant growth regulators) has negatively influenced proliferation of shoots. The higher concentrations of the above have delayed the development of plantlets. Shoot multiplication was enhanced by coconut water with 2% being the best among 4 and 8% tested. Shoot organogenesis was not influenced by copper sulphate (0, 1.5, 3, 6, and 12 mgL−1) and silver nitrate (0.0, 0.5, 2.5, and 12.5 mgL−1) supplements and at higher concentrations of the above inhibited plant growth.

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