scholarly journals Testing cutinase produced by native Trichoderma isolate and its persistence in pod and flower surfaces on cocoa tree in South Sulawesi

2020 ◽  
Vol 486 ◽  
pp. 012160
Author(s):  
Muhammad Junaid ◽  
A Rosmana ◽  
Firman
Keyword(s):  
South Sulawesi ◽  
Trichoderma Isolate ◽  
Cocoa Tree

scholarly journals Identifikasi isolat Phytophthora asal kakao Identification of isolates of Phytophthora from cocoa

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Abu UMAYAH ◽  
Agus PURWANTARA
Keyword(s):  
Molecular Identification ◽  
Stem Canker ◽  
Leaf Blight ◽  
Phytophthora Palmivora ◽  
Seedling Blight ◽  
West Java ◽  
Phytophthora Spp ◽  
South Sulawesi ◽  
Cocoa Production ◽  
Cocoa Tree

Summary Phytophthora spp. are responsible for some serious diseases of cocoa including pod rot, stem canker, leaf blight, seedling blight, and chupon wilt. Eight species of Phytophthora have been isolated from diseased cocoa worldwide, even though only three species cause most losses in cocoa production.  Twenty isolates of  Phytophthora sp. were isolated from various parts of the cocoa tree collected from six cocoa producing provinces in Indonesia, viz. North Sumatera, Lampung, West Java, East Java, South Sulawesi and Southeast Sulawesi.  All isolates were then identified using their morphological charac-teristics and it was concluded that all of the isolates are Phytophthora palmivora. This identification was then confirmed with molecular identification by amplification of ITS of rDNA of the isolates with primers ITS 4 and ITS 5, followed by restriction of the amplicon with enzymes.  The molecular identification confirmed that all isolates are P. palmivora. Ringkasan Phytophthora spp. merupakan penyebab beberapa penyakit penting pada kakao, termasuk busuk buah, kanker batang, hawar daun, hawar bibit, dan layu tunas air.  Delapan spesies Phytophthora telah berhasil diisolasi dari tanaman kakao sakit di seluruh dunia, meskipun hanya tiga spesies yang meng-akibatkan kehilangan produksi kakao yang nyata.  Dua puluh isolat Phytophthora sp. telah diisolasi dari berbagai bagian tanaman kakao yang dikumpulkan dari enam provinsi sentra produksi kakao di Indonesia, yaitu Sumatera Utara, Lampung, Jawa Barat, Jawa Timur, Sulawesi Selatan dan Sulawesi Tenggara.  Semua isolat diidentifikasi berdasarkan sifat-sifat morfologi dan dapat disimpulkan bahwa semua isolat adalah Phytophthora palmivora.  Identifikasi selanjutnya dilakukan secara molekuler dengan amplifikasi daerah ITS dari rDNA isolat menggunakan pasangan primer ITS 4 dan ITS 5, kemudian diikuti dengan pemotongan amplikon menggunakan enzim restriksi. Identifikasi molekuler juga menun-jukkan bahwa semua isolat Phytophthora penyebab penyakit pada kakao adalah P. palmivora.

scholarly journals Identifikasi isolat Phytophthora asal kakao Identification of isolates of Phytophthora from cocoa

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Abu UMAYAH ◽  
Agus PURWANTARA
Keyword(s):  
Molecular Identification ◽  
Stem Canker ◽  
Leaf Blight ◽  
Phytophthora Palmivora ◽  
Seedling Blight ◽  
West Java ◽  
Phytophthora Spp ◽  
South Sulawesi ◽  
Cocoa Production ◽  
Cocoa Tree

Summary Phytophthora spp. are responsible for some serious diseases of cocoa including pod rot, stem canker, leaf blight, seedling blight, and chupon wilt. Eight species of Phytophthora have been isolated from diseased cocoa worldwide, even though only three species cause most losses in cocoa production.  Twenty isolates of  Phytophthora sp. were isolated from various parts of the cocoa tree collected from six cocoa producing provinces in Indonesia, viz. North Sumatera, Lampung, West Java, East Java, South Sulawesi and Southeast Sulawesi.  All isolates were then identified using their morphological charac-teristics and it was concluded that all of the isolates are Phytophthora palmivora. This identification was then confirmed with molecular identification by amplification of ITS of rDNA of the isolates with primers ITS 4 and ITS 5, followed by restriction of the amplicon with enzymes.  The molecular identification confirmed that all isolates are P. palmivora. Ringkasan Phytophthora spp. merupakan penyebab beberapa penyakit penting pada kakao, termasuk busuk buah, kanker batang, hawar daun, hawar bibit, dan layu tunas air.  Delapan spesies Phytophthora telah berhasil diisolasi dari tanaman kakao sakit di seluruh dunia, meskipun hanya tiga spesies yang meng-akibatkan kehilangan produksi kakao yang nyata.  Dua puluh isolat Phytophthora sp. telah diisolasi dari berbagai bagian tanaman kakao yang dikumpulkan dari enam provinsi sentra produksi kakao di Indonesia, yaitu Sumatera Utara, Lampung, Jawa Barat, Jawa Timur, Sulawesi Selatan dan Sulawesi Tenggara.  Semua isolat diidentifikasi berdasarkan sifat-sifat morfologi dan dapat disimpulkan bahwa semua isolat adalah Phytophthora palmivora.  Identifikasi selanjutnya dilakukan secara molekuler dengan amplifikasi daerah ITS dari rDNA isolat menggunakan pasangan primer ITS 4 dan ITS 5, kemudian diikuti dengan pemotongan amplikon menggunakan enzim restriksi. Identifikasi molekuler juga menun-jukkan bahwa semua isolat Phytophthora penyebab penyakit pada kakao adalah P. palmivora.

Immaterial Cost and Production: Maximum Production Cost Level Through Marginal Approach

2020 ◽  
Vol 8 (2) ◽  
pp. 128-149
Author(s):  
Dini Maulana Lestari
Keyword(s):  
Quantitative Study ◽  
Marginal Cost ◽  
Production Cost ◽  
Maximum Level ◽  
Production Costs ◽  
Total Production ◽  
Sugar Factory ◽  
Maximum Production ◽  
Cost Approach ◽  
South Sulawesi

This paper will discuss about the immaterial costs and production yields at one of the refined sugar factory companies in Makassar, South Sulawesi. The theory is based on the fact that Immaterial is a cost that is almsgiving, meaning costs that are outside of the basic costs of the company in producing production, so this research aims to find out: (1) what is the production cost needed to produce this production, (2) the maximum level of production at company from 2013 to 2017. This type of research is a quantitative study because it uses a questionnaire in the form of values ​​that are processed using the marginal cost approach formula. The results of the analysis show that (1) the maximum level of production costs occurred in 2016 amounting to 6,912 with an Immaterial cost of Rp. 2,481,796,800 and the total production produced is 359,077.3 tons (2) The required workforce with the total production produced is 359,077.3 tones of 180 people including the maximum production point which means that the lowest value is achieved (optimal).    

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