Determination of CAST gene polymorphism in sheep of the Volgograd breed

Objective.To characterize cumulative joint damage (CJD) patterns in rheumatoid arthritis (RA) and determine their associations with demographic/clinical features and HLA-DRB1 gene polymorphism.Methods.Hand and foot radiographs were obtained from 404 patients with RA. CJD patterns were determined by 3 derivations from Sharp/van der Heijde scores, obtained by the mathematical division of scores for hands/feet (Sharp-h/f score), fingers/wrists (Sharp-f/w score), and erosion/space narrowing (Sharp-e/sn score), respectively. DNA and serum were obtained for determination of HLA-DRB1 polymorphism, rheumatoid factor (RF), and anticitrullinated protein antibodies (ACPA).Results.Patients with wrist-dominant CJD pattern were more likely to have severe RA than those with finger-dominant pattern (68.4% vs 46.0%; p = 0.036) as were those with foot-dominant vs hand-dominant CJD pattern (76.5% vs 56.4%; p = 0.044). HLA-DRB1 shared epitope (SE) alleles were associated with erosion-dominant CJD pattern (p = 0.021). Patients with erosion-dominant CJD pattern had higher levels of RF and ACPA than those with space-narrowing–dominant CJD pattern (median RF 71.35 U/ml vs 22.05 U/ml, respectively; p = 0.003; median ACPA 187.9 U/ml vs 143.2 U/ml, respectively; p < 0.001). The majority of triple-positive patients (SE+, RF+, ACPA+) had erosion-dominant CJD pattern (62.3%) while the majority of triple-negative patients (SE–, FR–, ACPA–) had space narrowing–dominant CJD pattern (75%; p = 0.017). ACPA was associated with HLA-DRB1 SE alleles (p < 0.05). Patients with foot-dominant CJD pattern were taller than those with hand-dominant CJD pattern (p = 0.002); those with erosion-dominant CJD pattern had higher weight and body mass index than those with space narrowing–dominant CJD pattern (p = 0.014, p = 0.001).Conclusion.CJD patterns were associated with disease severity, HLA-DRB1 SE status, presence and titer of ACPA and RF, and morphometric features.

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Insight in Leptin Gene Polymorphism and Impact on Milk Traits in Autochtonous Busha Cattle

Acta veterinaria ◽

10.2478/acve-2019-0012 ◽

2019 ◽

Vol 69 (2) ◽

pp. 153-163 ◽

Cited By ~ 1

Author(s):

Milan Maletić ◽

Nevres Paprikić ◽

Miodrag Lazarević ◽

Aida Hodžić ◽

Vesna Davidović ◽

...

Keyword(s):

Gene Polymorphism ◽

Freezing Point ◽

Cattle Breed ◽

Milk Traits ◽

Native Breed ◽

Cell Counts ◽

Pcr Rflp ◽

Endangered Population ◽

High Production

Abstract Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p=0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (−0.54°C) had significantly higher (p=0.004) average FPD values than those with AB genotype (−0.58°C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.

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THE ROLE OF POLYMORPHISMS OF MATRIX METALLOPROTEINASES’ POLYMORPHISMS 1 AND 12 IN THE FORMATION OF WHEEZING SYNDROME AMONG CHILDREN WITH RECURRENT BRONCHITIS

Wiadomości Lekarskie ◽

10.36740/wlek202107108 ◽

2021 ◽

Vol 74 (7) ◽

pp. 1595-1599

Author(s):

Maryna I. Strelkova ◽

Ganna S. Senatorova ◽

Valentin V. Polyakov

Keyword(s):

Matrix Metalloproteinases ◽

Gene Polymorphism ◽

Lower Risk ◽

Comparison Group ◽

Allergic Inflammation ◽

Gene Families ◽

Acute Bronchitis ◽

Dominant Model

The aim: Matrix metalloproteinases (MMP) play an important role in the architecture and remodeling of the lungs. There are 2 gene families of MMP among significantly different genes – MMP-1 and MMP-12, which are closely related to the pathophysiological processes of allergic inflammation, damage and restoration of tissues and the body’s defense against pathogens. Materials and methods: 70 examined children were divided into 2 groups: 37 children who had acute recurrent bronchitis complicated by wheezing syndrome, the comparison group included 33 children with acute bronchitis. The determination of gene polymorphism was carried out using ELISA analysis. Results: In the dominant model, carriers of the 2G allele genotypes had 3,45 times lower risk of wheezing syndrome compared with patients with the 1G/1G genotype (OR = 3,45, 95% CI: 1,07-11.15, p<0,05). In the dominant model, carriers of G-allele genotypes had a 4,2-fold lower risk of wheezing syndrome compared with patients with the AA genotype (OR = 4,2; 95% CI (CI) = 1,09- 16,09; p <0,05). Conclusions: Polymorphism rs1799750 in the MMP-1 gene increases the risk of developing the wheezing syndrome among children with acute recurrent bronchitis in 3,5 times. The rs2276109 polymorphism in the MMP-12 gene reduces the risk of wheezing syndrome by 4,2 times among children with acute recurrent bronchitis.

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Correlation between porcine CAST gene polymorphism with muscle fiber histological traits and carcass characteristics

Hereditas (Beijing) ◽

10.1360/yc-007-0065 ◽

2007 ◽

Vol 29 (01) ◽

pp. 65 ◽

Cited By ~ 1

Author(s):

Yan-Qun WU

Keyword(s):

Gene Polymorphism ◽

Muscle Fiber ◽

Carcass Characteristics ◽

Cast Gene

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Interleukin-4 −33 C/T Gene Polymorphisms are not Associated with Type 2 Diabetes Mellitus

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.5419 ◽

2020 ◽

Vol 8 (A) ◽

pp. 833-836

Author(s):

Milahayati Daulay ◽

Mutiara Indah Sari

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Gene Polymorphism ◽

Gene Polymorphisms ◽

Interleukin 4 ◽

Healthy Control ◽

Different Populations

BACKGROUND: Various studies have shown variations in the interleukin-4 (IL) gene associated with type 2 diabetes mellitus (T2DM) in different populations and ethnicities. AIM: The objective of this study was to determine the genetic variation of the IL-4 −33 C/T gene in some T2DM patients compared with healthy control in Medan, Sumatera Utara province, Indonesia. METHODS: DNA from T2DM patients and healthy control was isolated, then the determination of the IL-4 −33 C/T gene polymorphism was carried out using the polymerase chain reaction-restriction fragment length polymorphism technique. RESULTS: In all samples of this study, IL-4 −33 C/T gene polymorphisms were found in patients with T2DM and healthy control but were not statistically significant. CONCLUSION: The IL-4 −33 C/T gene polymorphism has no relationship with the incidence of T2DM in a portion of our population in Medan, Sumatera Utara province, Indonesia.

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Polymorphism of genes of Growth Hormone (GH) and Calpastatin (CAST) in wool-and-meat producing sheep

Glavnyj zootehnik (Head of Animal Breeding) ◽

10.33920/sel-03-2007-01 ◽

2020 ◽

pp. 6-11

Author(s):

L. Skorykh ◽

I. Fominova ◽

E. Surzhikova ◽

D. Kovalenko

Keyword(s):

Growth Hormone ◽

Gene Polymorphism ◽

Animal Husbandry ◽

Breeding Programs ◽

Biochemical Processes ◽

Gh Gene ◽

Meat Productivity ◽

Physiological And Biochemical ◽

Modern Technologies ◽

Cast Gene

At present, modern technologies involving the use of DNA markers, which are widely used in national breeding programs in a number of countries with developed animal husbandry are of considerable interest and have a signifi cant infl uence on improving productivity traits. A signifi cant number of genes associated with meat productivity and meat quality have been identifi ed. In sheep breeding such research has been developed only recently. Due to the growing interest in the production of young mutton and lamb, priority is given to the study of genes that control meat productivity. However, the most popular are genetic markers that are interconnected with candidate genes, whose protein products play a signifi cant role in the formation or regulation of physiological and biochemical processes. Alleles of the Growth Hormone (GH) gene and Calpastatin (CAST) gene are considered as potential markers of sheep meat productivity. In this regard, the purpose of this work was to study the polymorphism of GH and CAST genes in crossbred wool-and-meat producing sheep (1/2 Poll Dorset × 1/2 North Caucasian wool-and-meat producing breed) bred in the Stavropol Territory. The analysis of Somatotropin and Calpastatin gene polymorphism was performed by PCR-PDRF. When studying GH gene polymorphism, the genotypes AA, AB and BB have been found, the frequency of occurrence of which was 29,7; 42,8 and 27,5 %, respectively. Allele A prevailed in the studied animals – 0,786, allele B was – 0,214. The CAST gene was represented by two genotypes MM and MN (the NN genotype was absent in the animals of this group) with a frequency of 87,9 and 12,1 %, respectively. The frequency of the M allele was high at 0,94, while the n allele was low at 0,06. The results obtained during the researches indicate the establishment of a variety of allelic variants of Somatotropin and Calpastatin genes in wool-and-meat producing sheep. Further research will be aimed at studying the association of identifi ed polymorphisms with economically useful traits.

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