Effects of In-Ovo injection of Biotin on chick’s embryonic development and physiological traits

Sulfur amino acids are typically the first-limiting amino acids (AA) used in protein metabolism in poultry. Therefore, we hypothesized that their utilization in the pre-hatch period would affect embryonic development, IGF-I and TLR4 gene expression, antioxidant status, serum biochemical profile, and jejunum histomorphometry of newly hatched Ross broiler chicks incubated under heat stress conditions. A total of 150 fertile broiler eggs were subjected to heat stress (39.6 °C for 6 h/d) from d10 until d18 and injected at d 17.5 of incubation with methionine and cysteine (Met-Cys) at a dose of 5.90 mg l-methionine plus 3.40 mg l-cysteine. The effects of Met-Cys administration were examined and compared with the control (Non-injected group) and 0.75% NaCl injected group. The results showed that no significant differences among all groups in serum protein profiles (total protein, albumin, globulin, and albumin/globulin ratio) and creatine kinase were observed. The level of heat shock protein-90 was decreased with Met-Cys In ovo injection. The In ovo injection of Met-Cys also improved the values of total antioxidants capacity and glutathione in examined tissues. At the same time, an increase in fold change mRNA abundance of IGF-I and TLR4 was observed after Met-Cys injection in tested tissues. Finally, an increase of 29% in villus area was found after Met-Cys injection compared to the control group. In conclusion, the In ovo injection of Met-Cys resulted in improved embryonic development, IGF-I and TLR4 gene expression, antioxidant status and jejunum histomorphometry of newly hatched broiler chicks exposed to heat stress during incubation.

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INFLUENCE OF IN OVO INJECTION OF L-ARGININE ON HATCHABILITY AND SOME PHYSIOLOGICAL TRAITS OF INSHAS LOCAL BREED CHICKEN

Egyptian Poultry Science Journal ◽

10.21608/epsj.2018.22925 ◽

2018 ◽

Vol 38 (4) ◽

pp. 1155-1169

Author(s):

aber eshra

Keyword(s):

Physiological Traits ◽

In Ovo ◽

Local Breed ◽

In Ovo Injection

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In ovo injection of branched‐chain amino acids: Embryonic development, hatchability and hatching quality of turkey poults

Journal of Animal Physiology and Animal Nutrition ◽

10.1111/jpn.13111 ◽

2019 ◽

Author(s):

Canan Kop‐Bozbay ◽

Nuh Ocak

Keyword(s):

Amino Acids ◽

Embryonic Development ◽

Branched Chain Amino Acids ◽

In Ovo ◽

Turkey Poults ◽

In Ovo Injection ◽

Branched Chain

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Effects of in ovo injection of Astragalus kahericus polysaccharide on early growth, carcass weights and blood metabolites in broiler chickens

Animal Biotechnology ◽

10.1080/10495398.2021.1924763 ◽

2021 ◽

pp. 1-7

Author(s):

Hussin H. El-Fakhrany ◽

Zenat A. Ibrahim ◽

Elwy A. Ashour ◽

Ali Osman ◽

Mahmoud Alagawany

Keyword(s):

Broiler Chickens ◽

Early Growth ◽

Blood Metabolites ◽

In Ovo ◽

In Ovo Injection

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In Ovo Injection of GABA Can Help Body Weight Gain at Hatch, Increase Chick Weight to Egg Weight Ratio, and Improve Broiler Heat Resistance

Animals ◽

10.3390/ani11051364 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1364

Author(s):

Chris Major Ncho ◽

Akshat Goel ◽

Chae-Mi Jeong ◽

Mohamed Youssouf ◽

Yang-Ho Choi

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Antioxidant Status ◽

Body Weight Gain ◽

Weight Ratio ◽

The Other ◽

Mrna Levels ◽

In Ovo ◽

Total Antioxidant ◽

In Ovo Injection

The aim of this study was to explore the outcomes of an in ovo GABA injection in broilers challenged with HS. In Experiment 1, 210 Arbor Acres eggs were allocated to five treatments: no-injection, and in ovo injection of 0.6 mL of 0%, 5%, 10%, or 20% of GABA. Hatchling weight and CWEWR were significantly increased in the 5% GABA group. In ovo, injection of 10% GABA solution caused a significant decrease in plasma cholesterol and increased plasma total antioxidant capacity of hatchlings. Experiment 2 was conducted with 126 fertile Arbor Acres eggs distributed into one of two groups. At 17.5 days of incubation, one received no injection, and the other was fed 0.6 mL of 10% GABA. On day 10, one subgroup (4 replicates * 3 birds) from each treatment was submitted to HS (38 ± 1 °C for 3 h) while the other was kept at a thermoneutral temperature (29 ± 1 °C). An in ovo injection of GABA significantly increased total antioxidant capacity, but reduced malondialdehyde levels, hepatic mRNA levels of HSP70, FAS, and L-FABP with HS. In conclusion, an in ovo GABA injection improves CWEWR and antioxidant status at hatch, and enhances antioxidant status while downregulating the expression of HSP70 and fatty acid metabolism-related genes in young chicks under HS.

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