scholarly journals Optimization of Upstream Processing for the Production of Bioethanol from Leucaena leucocephala Seeds using Kluyveromyces marxianus UniMAP 1-1

2021 ◽  
Vol 765 (1) ◽  
pp. 012053
Author(s):  
M Z Ishak ◽  
M L Kuan ◽  
K S K Ismail
Keyword(s):  
Kluyveromyces Marxianus ◽  
Leucaena Leucocephala ◽  
Upstream Processing

Forage palatability of Broussonetia papyrifera an invasive species in Ghana: Relative preference and palatability by sheep and goats

2018 ◽  
Vol 2 (2) ◽  
pp. 63-70 ◽  
Author(s):  
R. Obour
Keyword(s):  
Invasive Species ◽  
Leucaena Leucocephala ◽  
Condensed Tannin ◽  
Block Design ◽  
Wet Season ◽  
Broussonetia Papyrifera ◽  
Sheep And Goats ◽  
Relative Preference ◽  
Exotic Tree ◽  
High Level

Broussonetia papyrifera is an exotic tree widely grown for paper production. Due to its prolific regeneration it has invaded forestcanopy gaps and degraded farmlands and has now become an invasive species in Ghana. In enhancing its value for use the plantwas evaluated as potential forage for grazing animals vis-à-vis other two existing forage plants: Ficus exasperata and Leucaenaleucocephala.The study assessed the palatability and preference of Broussonetia papyrifera using sheep and goats for the wet anddry seasons.The species were assessed in indoor pen feeding trials using eight-unit (3×3 m) pens with the cafeteria method.The amount of forage offered was 100g (fresh material) in all instances for each species and for ten minutes. Adesign basedon 3×2×2 factorial in Randomized Complete Block Design (RCBD) was used to test the differences in palatability betweenthe three forage species.Results revealed that palatability was higher (P<0.05) in Leucaena leucocephala compared with Ficusexasperata and Broussonetia papyrifera for sheep and goats across seasons. The trend shown might be the result of the effectsof familiarity with the Leucaena leucocephala since animals tend to select plants that are familiar than newly introduced andunfamiliar plants. The study also revealed high level of condensed tannin (CT) in Broussonetia papyrifera which might haveinterfered with forage intake by the animals.There were no significant differences in palatability of Broussonetia papyrifera forgoat in both dry and wet season interactions and Ficus exasperata for goat in both dry and wet season interactions (P>0.05).Thestudy concluded that Broussonetia papyrifera could be a potential feed for both sheep and goats across seasons.The researchrecommended that livestock farmers should incorporate Broussonetia papyrifera feed into their programmes for both sheep andgoats and should be introduced to animals from infancy so that it may become a familiar feed for them.

Uji Aktivitas Antihiperglikemik Ekstrak Daun Petai Cina (Leucaena leucocephala (Lam.) de Wit) pada Mencit Induksi Aloksan

2019 ◽  
Vol 16 (1) ◽  
pp. 107
Author(s):  
Jena Hayu Widyasti ◽  
Fitri Kurniasari
Keyword(s):  
Leucaena Leucocephala ◽  
Diabetes Melitus

Diabetes melitus merupakan suatu penyakit dengan adanya gangguan sekresi insulin baik karena penurunan sensitifitas maupun kerusakan pada sel beta. Tujuan penelitian ini adalah membuktikan ekstrak daun petai cina (Leucaena leucocephala (Lam.) de Wit) memiliki aktivitas menurunkan kadar gula darah mencit hiperglikemik pada dosis yang efektif. Penelitian antihiperglikemik ini menggunakan mencit putih sebanyak 30 ekor. Pengujian aktivitas antihiperglikemik dilakukan pada 6 kelompok perlakuan yaitu kelompok I (kontrol normal), kelompok II (kontrol negatif, induksi aloksan 200 mg/kg BB dan akuades), kelompok III (induksi aloksan dan glibenklamida 10 mg/kg BB), kelompok IV (induksi aloksan dan ekstrak daun petai cina 200 mg/kg BB), kelompok V (induksi aloksan dan ekstrak daun petai cina 400 mg/kg BB), kelompok VI (induksi aloksan dan ekstrak daun petai cina 300 mg/kg BB). Semua kelompok perlakuan diberikan perlakuan tersebut selama 14 hari dan dilakukan pengukuran kadar gula darah pada hari ke-0, 3, 10, dan 17. Aktivitas antihiperglikemik ekstrak daun petai cina ditunjukkan dengan cara menghitung daya hipoglikemik masing-masing perlakuan. Hasil penelitian menunjukkan bahwa ekstrak etanol daun petai cina mempunyai aktivitas antihiperglikemik pada mencit yang diinduksi aloksan. Pada dosis uji ekstrak etanol daun petai cina 600 mg/kg BB mempunyai aktivitas antihiperglikemik yang efektif dibanding dengan dosis 400 mg/kg BB dan 200 mg/kg BB yang sebanding dengan kelompok kontrol positif.

Kluyveromyces marxianus as a Probiotic Yeast: A Mini-review

2020 ◽  
Vol 16 (8) ◽  
pp. 1163-1169
Author(s):  
Aziz Homayouni-Rad ◽  
Aslan Azizi ◽  
Parvin Oroojzadeh ◽  
Hadi Pourjafar
Keyword(s):  
Kluyveromyces Marxianus ◽  
Yeast Species ◽  
Human Life ◽  
Saccharomyces Boulardii ◽  
Biotechnological Products ◽  
Probiotic Yeast ◽  
Ancient Times ◽  
Main Components ◽  
Micro Organisms ◽  
Harmful Effects

Background: Yeasts play diverse roles in human life. Since ancient times, these micro organisms have been used to produce food products and beverages including bread and beer. Nowadays, the biotechnological products of yeast are some of the main components of commercial products. Objective: Some species of yeast such as Saccharomyces cerevisiae and Saccharomyces boulardii are recognized as probiotic yeast with extensive applications in the food and drug industries. However, certain species like Kluyveromyces marxianus are still not recognized as probiotic micro organisms despite their widespread industrial usage. In this study, the application of K. marxianus in preparing food and the medicinal product was reviewed in terms of its beneficial or harmful effects. Methods: Pub Med, Google Scholar, Scopus, and Science Direct databases were searched by using “Probiotics”, “Yeast”, and “Kluyveromyces marxianus”. Results: The findings suggest that K. marxianus can be recognized as a probiotic yeast species. Conclusion: It can be concluded that K. marxianus may be considered as a probiotic micro organism with a variety of commercial and medical applications.

scholarly journals Farinha de folha de leucena (Leucaena leucocephala Lam. de wit) como fonte de proteína para juvenis de tambaqui (Colossoma macropomum CUVIER, 1818)

2013 ◽  
Vol 43 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Geraldo Pereira Junior ◽  
Manoel Pereira Filho ◽  
Rodrigo Roubach ◽  
Paula de Sousa Barbosa ◽  
Eduardo Shimoda
Keyword(s):  
Leucaena Leucocephala ◽  
Colossoma Macropomum ◽  
Em 12

O tambaqui (Colossoma macropomum) é um peixe onívoro, natural da bacia amazônica, que possui elevado valor comercial. Características de rusticidade e desempenho produtivo destacam esta espécie para criação em cativeiro. Contudo, em criações comerciais de peixes, os custos com alimentação podem corresponder de 60 a 80% dos custos totais de produção, sendo a proteína o nutriente mais caro da dieta. O objetivo deste trabalho foi avaliar o desempenho produtivo de juvenis de tambaqui alimentados com rações contendo farinha de folha de leucena como fonte protéica. 240 juvenis foram distribuídos em 12 aquários experimentais (350 L), em um delineamento experimental inteiramente casualizado com quatro tratamentos (0%, 8%, 16%, 24% de inclusão de farinha de folha de leucena na ração) e três repetições. Foram determinados o ganho de peso, conversão alimentar aparente, taxa de crescimento específico, taxa de eficiência protéica e custo de produção do quilograma de peso vivo ganho. Para as variáveis estudadas, não houve diferença significativa (p>0,05) entre os tratamentos, indicando que é possível incluir até 24% de farinha de folha de leucena em rações para juvenis de tambaqui, sem comprometimento das variáveis estudadas, embora a substituição não tenha representado redução no custo de produção do quilograma de peixe.

Bacterial endosymbiont inhabiting Leucaena leucocephala leaves and their antioxidant and antidiabetic potential

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sridevi Chigurupati ◽  
Shantini Vijayabalan ◽  
Kesavanarayanan Krishnan Selvarajan ◽  
Ahmad Alhowail ◽  
Fatema Kauser
Keyword(s):  
Free Radical ◽  
Crude Extract ◽  
Leucaena Leucocephala ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
16S Rrna Gene Sequencing ◽  
Free Radical Scavenging ◽  
Rrna Gene ◽  
Standard Drug

AbstractObjectivesResearch on endosymbionts is emerging globally and is considered as a potential source of bioactive phytochemicals. The present study examines the antioxidant and antidiabetic of the endophytic crude extract isolated from Leucaena leucocephala leaves.MethodsEndophytic bacteria were isolated from the leaves of L. leucocephala and 16S rRNA gene sequencing was used to establish their identity. The in vitro antioxidant effect of endophytic crude extract (LL) was evaluated using 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) free radical scavenging methods. The in vitro antidiabetic properties of LL were evaluated using α-amylase and α-glucosidase enzyme inhibition assay.ResultsThe isolated endophytic bacteria were identified as Cronobacter sakazakii. LL displayed potent free radical scavenging effect against ABTS and DPPH radicals with an inhibitory concentration 50% (IC50) value of 17.49 ± 0.06 and 11.3 ± 0.1 μg/mL respectively. LL exhibited α-amylase and α-glucosidase inhibition with an IC50 value of 23.3 ± 0.08 and 23.4 ± 0.1 μg/mL respectively compared to the standard drug (acarbose). Both glucose loaded normoglycemic rats and STZ induced diabetic rats treated with LL (200 mg/kg) exhibited a considerable reduction in blood glucose levels p<0.01 after 8 h of treatment when compared to normal and diabetic control rats respectively.ConclusionsThus, the study shows that LL has a wellspring of natural source of antioxidants, and antidiabetic agents and phytoconstituents present in endophytes could be the rich source for bioactive compounds.

scholarly journals Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 62
Author(s):  
Konstantins Dubencovs ◽  
Janis Liepins ◽  
Arturs Suleiko ◽  
Anastasija Suleiko ◽  
Reinis Vangravs ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reaction Medium ◽  
Value Added ◽  
Batch Cultivation ◽  
Batch Processes ◽  
Biomass Growth ◽  
High Cell ◽  
Media Composition ◽  
Fed Batch ◽  
Fed Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.

scholarly journals Correction to: Transcriptome analysis of the thermotolerant yeast Kluyveromyces marxianus CCT 7735 under ethanol stress

2021 ◽  
Author(s):  
Raphael Hermano Santos Diniz ◽  
Juan C. Villada ◽  
Mariana Caroline Tocantins Alvim ◽  
Pedro Marcus Pereira Vidigal ◽  
Nívea Moreira Vieira ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Kluyveromyces Marxianus ◽  
Ethanol Stress ◽  
Thermotolerant Yeast
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