High sensitivity and specificity Enzyme-Linked immunosorbent assay for detection of Acetaminophen in herbal tea and liquor

ABSTRACT The reactivities of sera from chronic chagasic patients against the trypomastigote excreted-secreted antigens (TESA) of Trypanosoma cruzi strains with different biodemes were analyzed by TESA-blot and TESA–enzyme-linked immunosorbent assay (ELISA). Although both tests presented high sensitivity and specificity, TESA-ELISA is more appropriate for screening a larger number of samples.

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Detection of Serum Antibodies to Ovine Progressive Pneumonia Virus in Sheep by Using a Caprine Arthritis-Encephalitis Virus Competitive-Inhibition Enzyme-Linked Immunosorbent Assay

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.10.5.862-865.2003 ◽

2003 ◽

Vol 10 (5) ◽

pp. 862-865 ◽

Cited By ~ 21

Author(s):

Lynn M. Herrmann ◽

William P. Cheevers ◽

Katherine L. Marshall ◽

Travis C. McGuire ◽

Melinda M. Hutton ◽

...

Keyword(s):

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Competitive Inhibition ◽

False Negative ◽

High Sensitivity ◽

The United States ◽

Encephalitis Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Caprine Arthritis Encephalitis Virus ◽

Ovine Progressive Pneumonia

ABSTRACT A competitive-inhibition enzyme-linked immunosorbent assay (cELISA) for detection of antibodies to the surface envelope (SU) of caprine arthritis-encephalitis virus (CAEV) was recently reported (L. M. Herrmann, W. P. Cheevers, T. C. McGuire, D. Scott Adams, M. M. Hutton, W. G. Gavin, and D. P. Knowles, Clin. Diagn. Lab. Immunol. 10:267-271, 2003). The cELISA utilizes CAEV-63 SU captured on microtiter plates using the monoclonal antibody (MAb) F7-299 and measures competitive displacement of binding of the anti-CAEV MAb GPB 74A by goat serum. The present study evaluated the CAEV cELISA for detection of antibodies to ovine progressive pneumonia virus (OPPV) in sheep. Three hundred thirty-two sera were randomly selected from 21,373 sheep sera collected throughout the United States to determine the sensitivity and specificity of cELISA and agar gel immunodiffusion (AGID) based on immunoprecipitation (IP) of [35S]methionine-labeled OPPV antigens as a standard of comparison. A positive cELISA test was defined as >20.9 percent inhibition (% I) of MAb 74A binding based on two standard deviations above the mean % I of 191 IP-negative sheep sera. At this cutoff, there were 2 of 141 false-negative sera (98.6% sensitivity) and 6 of 191 false-positive sera (96.9% specificity). Sensitivity and specificity values for IP-monitored AGID were comparable to those for cELISA for 314 of 332 sera with unambiguous AGID results. Concordant results by cELISA and IP resolved 16 of the 18 sera that were indeterminate by AGID. Additional studies evaluated cELISA by using 539 sera from a single OPPV-positive flock. Based on IP of 36 of these sera, there was one false-negative by cELISA among 21 IP-positive sera (95.5% sensitivity) and 0 of 15 false-positives (100% specificity). We conclude that the CAEV cELISA can be applied to detection of OPPV antibodies in sheep with high sensitivity and specificity.

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Integration of Liquid Chromatography with Immunoassay: An Approach Combining the Strengths of Both Methods

Journal of AOAC International ◽

10.1093/jaoac/77.5.1275 ◽

1994 ◽

Vol 77 (5) ◽

pp. 1275-1287 ◽

Cited By ~ 7

Author(s):

Petra M Krämer ◽

Qing X Li ◽

Bruce D Hammock

Keyword(s):

Liquid Chromatography ◽

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Uv Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Multiresidue Analysis ◽

Selective Method ◽

Separation Power ◽

Immunochemical Detection ◽

Very High

Abstract The integration of liquid chromatography (LC) with immunochemical detection combines the superior separation power of LC and the sensitivity and specificity of immunoassays. This approach is shown with 3 LC systems (Perkin-Elmer, C18 RP, 4.6 mm; Varian, C18 RP, 1 mm microbore; Michrom, C18 RP, 1 mm microbore) Integrated with an enzyme-linked immunosorbent assay (ELISA) selective for five 4-nitrophenols. The nitrophenols were separated with the 3 LC systems with isocratic runs of 15 to 20 min. Microbore LC separation showed a 10-20 times reduction in solvent amount compared to conventional separation. LC–immunoassay was about 8- to 10-fold more sensitive compared with LC with UV detection. Integrated LC–immunoassay proved to be a very selective method when 2-methylphenol was injected with an equimolar mixture of 2-amino-4-nitrophenol and 3-methyl-4-nrtrophenol; 2-methy I phenol does not crossreact with the serum used. Only 2 peaks could be seen in the detection, even when 2-methylphenol was present in very high amounts (3000 pmol). Further, the EUSA-LC detection proved to be selective and sensitive for complex matrixes. 2-Amlno-4-nitrophenol was clearly identified in spiked extracts of soil and plant, even when a very small amount (2.4 ng) was injected. Although LC–immunoassay is more labor intensive than LC with UV detection, it offers great advantages in multiresidue analysis and is generally applicable for peak confirmation.

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Assessment of the Sensitivity and Specificity of Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Mycobacterial Antibodies in Bovine Tuberculosis

Journal of Veterinary Medicine Series B ◽

10.1111/j.1439-0450.1987.tb00377.x ◽

1987 ◽

Vol 34 (1-10) ◽

pp. 119-125 ◽

Cited By ~ 17

Author(s):

Viviana Ritacco ◽

. Isabel N. de Kantor ◽

Lucía Barrera ◽

Alfredo Nader ◽

Amelia Bernardelli ◽

...

Keyword(s):

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Bovine Tuberculosis ◽

Enzyme Linked Immunosorbent Assay

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Interpretations of Antibody Responses toSalmonella enterica Serotype Enteritidis gm Flagellin in Poultry Flocks Are Enhanced by a Kinetics-Based Enzyme-Linked Immunosorbent Assay

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.5.4.550-555.1998 ◽

1998 ◽

Vol 5 (4) ◽

pp. 550-555 ◽

Cited By ~ 8

Author(s):

Patrick L. McDonough ◽

Richard H. Jacobson ◽

John F. Timoney ◽

Ahmed Mutalib ◽

David C. Kradel ◽

...

Keyword(s):

Immunosorbent Assay ◽

High Sensitivity ◽

High Specificity ◽

Enzyme Linked Immunosorbent Assay ◽

Analytical Sensitivity ◽

Department Of Agriculture ◽

Trace Back ◽

Elisa Format ◽

Commercial Poultry ◽

Computer Controlled

ABSTRACT Many regulatory and diagnostic programs for the detection ofSalmonella enterica serotype Enteritidis infection in commercial poultry flocks have relied on rapid Pullorum agglutination tests to screen birds because of the shared antigens of S. enterica Enteritidis and S. enterica Pullorum and Gallinarum; however, the use of the enzyme-linked immunosorbent assay (ELISA) format affords better analytical sensitivity than crude agglutination tests. In this study, we adapted our earlier conventional indirect ELISA, using gm flagellin as the antigen, to a kinetics-based, computer-controlled ELISA (KELA). The KELA was used to screen for flagellin antibody from three commercial flocks: (i) a large flock involved in a U.S. Department of Agriculture trace back from a humanS. enterica Enteritidis foodborne outbreak (n = 3,209), (ii) a flock infected with the endemicS. enterica Enteritidis serotype but which also had multiple other salmonella serotypes (n = 65), and (iii) an S. enterica Pullorum-infected flock (n = 12). The first flock (S. entericaEnteritidis prevalence of 2.45% based on culture) provided a field test of the KELA and allowed the calculation of diagnostic sensitivity (D-Sn) and diagnostic specificity (D-Sp). With a cutoff of 10 (used for screening flocks [i.e., high sensitivity]), the KELA has a D-Sn of 95.2% and a D-Sp of 18.5%; with a cutoff of 140 (used in confirmatory flock testing [i.e., high specificity]), the KELA has a D-Sn of 28.0% and a D-Sp of 99.1%. We found that with a cutoff of 60 (D-Sn = 63.1%; D-Sp = 91.6%), we could eliminate reactions in the KELA caused by other non-S. enterica Enteritidis salmonellae. The KELA was also compared to two commercial rapid Pullorum tests, the Solvay (D-Sn = 94.9%; D-Sp = 55.5%) and the Vineland (D-Sn = 62.0%; D-Sp = 75.3%).

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Analysis ofLeishmaniamimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis

Parasitology ◽

10.1017/s0031182018000720 ◽

2018 ◽

Vol 145 (14) ◽

pp. 1938-1948 ◽

Cited By ~ 1

Author(s):

Lígia Moraes Barizon de Souza ◽

Vanete Thomaz Soccol ◽

Ricardo Rasmussen Petterle ◽

Michelle D. Bates ◽

Paul A. Bates

Keyword(s):

Cell Surface ◽

Cutaneous Leishmaniasis ◽

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Individuals ◽

Cell Surfaces ◽

Cell Markers ◽

Antibody Titres

AbstractOligosaccharides are broadly present onLeishmaniacell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Galα1-3Galβis the immunodominant saccharide inLeishmaniacell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-α-galactosyl (α-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Galα1-3Gal terminal fraction (Galα1-3Galβ1-4GlcNAc-HAS and Galα1-3Gal-HAS) and one Galα1-3Gal NGP analogue (Galα1-3Galβ1-3GlcNAc-HAS) were used as antigens. Means of anti-α-Gal antibody titres of CL patients were significantly higher (P< 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.

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Enzyme-Linked Immunosorbent Assay and Microbiologic Culture for Diagnosis of Staphylococcus aureus Intramammary Infection in Cows

Journal of Food Protection ◽

10.4315/0362-028x-59.1.6 ◽

1996 ◽

Vol 59 (1) ◽

pp. 6-10 ◽

Cited By ~ 3

Author(s):

PAUL C. BARTLETT ◽

RONALD J. ERSKINE ◽

PATRICK GASTON ◽

PHILIP M. SEARS ◽

HENDRICUS WILHELMUS HOUDIJK

Keyword(s):

Staphylococcus Aureus ◽

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Relative Sensitivity ◽

Elisa Test ◽

Enzyme Linked Immunosorbent Assay ◽

Infection Status ◽

Intramammary Infection ◽

Previous Infection ◽

Current Infection

Recent reports have indicated that the relative sensitivity and specificity of the ELISA test for detection of intramammary infection of cows with Staphylococcus aureus is not as high as originally reported. It has been suggested that antibodies measured by enzyme-linked immunosorbent assay (ELISA) more closely reflect previous infection status rather than current infection status, and that the delay in antibody formation following infection and the persistence of antibodies after elimination of infection may be responsible for some of the discrepancy observed between ELISA and bacterial culture results conducted on the same milk sample. This study (n = 209 cows) was undertaken to determine if an ELISA for S. aureus intramammary infection more closely reflects previous infection status than it does current infection status, and to ascertain whether correction of this time-delay factor substantially improves calculated values of ELISA relative sensitivity and specificity. Receiver-operator curves were constructed to compare different time-related definitions of microbiologic culture results used for comparison with ELISA results. A greater degree of curvature in receiver-operator curves indicated that ELISA results did more closely reflect culture results performed on milk samples taken 1 and 3 weeks previously. Insignificant improvement in sensitivity and specificity occurred when the database was limited to cows (n = 140) with milk production greater than 13.6 kg/day. However, values of sensitivity were all less than or equal to 90%, and values of specificity were all less than 54%.

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Rapid Detection of Salmonella enterica Serovar Choleraesuis in Blood Cultures by a Dot Blot Enzyme-Linked Immunosorbent Assay

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.7.6.977-979.2000 ◽

2000 ◽

Vol 7 (6) ◽

pp. 977-979 ◽

Cited By ~ 8

Author(s):

Kritsana Janyapoon ◽

Sunee Korbsrisate ◽

Hatairat Thamapa ◽

Sittichai Thongmin ◽

Suwattana Kanjanahareutai ◽

...

Keyword(s):

Monoclonal Antibody ◽

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Salmonella Enterica ◽

Rapid Detection ◽

Direct Detection ◽

Blood Cultures ◽

Enzyme Linked Immunosorbent Assay ◽

Biochemical Method ◽

Dot Blot

ABSTRACT A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella entericaserovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Choleraesuis were positive on testing by the ELISA. In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%.

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Diagnosis of Visceral Leishmaniasis by Enzyme-Linked Immunosorbent Assay Using Urine Samples

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.4.789-794.2002 ◽

2002 ◽

Vol 9 (4) ◽

pp. 789-794 ◽

Cited By ~ 3

Author(s):

Mohammad Zahidul Islam ◽

Makoto Itoh ◽

S. M. Shamsuzzaman ◽

Rusella Mirza ◽

Farzana Matin ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Leishmania Donovani ◽

Laboratory Diagnosis ◽

Urine Samples ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Serum Samples ◽

Crude Antigen

ABSTRACT A diagnostic method has been developed to detect anti-Leishmania donovani immunoglobulin G (IgG) in urine by enzyme-linked immunosorbent assay (ELISA). In measuring anti-L. donovani IgG, IgA, and IgM in urine, the method performed best in the detection of IgG. The sensitivity and specificity of the assay were determined with panels of urine samples from 62 visceral leishmaniasis (VL) patients, 59 healthy controls from areas of endemicity, 53 healthy controls from areas of nonendemicity, 59 malaria patients, 13 tuberculosis patients, 23 cutaneous leishmaniasis patients, and 7 patients with other diseases. Using L. donovani promastigote crude antigen, the test had 93.5% sensitivity (58 positives of 62 VL patient samples) and 89.3% specificity (191 negatives of 214 non-VL patient samples). The ELISA with acetone-treated L. donovani promastigote antigen raised the sensitivity and specificity to 95.0 and 95.3%, respectively. Western blot analysis revealed that most of the samples that cross-reacted with crude antigen in ELISA did not recognize any antigenic component of L. donovani crude antigen. We also checked 40 serum samples from the same group of VL patients for anti-L. donovani IgG and got 90.0% sensitivity with both crude and acetone-treated antigens. As collection of urine is much easier than collection of serum, the detection of anti-L. donovani IgG in urine with acetone-treated antigen will be useful in epidemiological studies. It could be an adjunct of laboratory diagnosis.

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