scholarly journals Microtubers production by using Temporary Immersion System (TIS) bioreactor to potato varieties

2021 ◽  
Vol 886 (1) ◽  
pp. 012005
Author(s):  
Septi Andriani ◽  
Luthfi Aziz Mahmud Siregar ◽  
Irda Safni
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Potato Seed ◽  
Temporary Immersion System ◽  
Temporary Immersion ◽  
Potato Varieties ◽  
Randomized Design ◽  
Tissue Culture Method ◽  
Certified Potato Seed

Abstract As the national potatoes growth has become lower from year to year, it is now known that the problems came from the lack of certified potato seed varieties and the minimum access to sophisticated technology to make a good potato variety. The solution that can be made is to utilize microtubers as an efficient factor. The purpose of this research is to find the most effective method in micro tubers cultivation in vitro by comparing the conventional tissue culture method and the Temporary Immersion System (TIS) bioreactor method to four different potato varieties (Granola L., Dayang Sumbi, Atlantic Malang, Maglia). This research uses a split-plot design with a completely randomized design by using two factorial. The result of this study shows that the microtubers in the multiplication and production step using the TIS bioreactor method has a higher average compared to the conventional tissue culture method. As the various details, Dayang Sumbi has the highest parameter such as most sprouts, primer roots, diameter, wet weights, and fastest time growth. Granola L. excel in planlet height and most tubers. Atlantic Malang in the most multiplication and nodus. Meanwhile Maglia excel in most leaves.

scholarly journals In Vitro Effect of Erythropoietin on the Spleen of The Polycythemic Mouse

Blood ◽  
1967 ◽  
Vol 29 (6) ◽  
pp. 852-858
Author(s):  
YASUSADA MIURA ◽  
FUMIMARO TAKAKU ◽  
KIKU NAKAO
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Stem Cell Differentiation ◽  
Mouse Spleen ◽  
In Vitro Method ◽  
Heme Synthesis ◽  
Tissue Culture Method ◽  
Vitro Effect

Abstract 1. An in vitro method to observe radiosensitivity of stem cells was developed in the present study. In vivo and in vitro effect of 60Co irradiation on the erythropoietin-induced stem cell differentiation into erythroblasts was observed, using a tissue culture method of polycythemic mouse spleen. Response to erythropoietin was demonstrated by an appearance of heme synthesis and erythroblasts in spleen fragments. 2. A significant correlation between the rate of appearance of erythroblasts and heme synthesis of the spleen fragments was observed. 3. After irradiation, marked impairment of both heme synthesis and production of erythroblasts was observed, yielding D37 values in the vicinity of 70 r in vivo and 120 r in vitro irradiation, respectively. 4. Marked recovery of erythropoietin-induced heme synthesis in the polycythemic mouse spleen was observed 9 days after 300 r irradiation, with an "overshooting" phenomenon on the 12th day.

scholarly journals Study of Seaweed Kappaphycus alvarezii Explants Growth in the Different Salinity Concentrations

2021 ◽  
Vol 13 (1) ◽  
pp. 97
Author(s):  
Muhammad Aris ◽  
Fatma Muchdar ◽  
Rusmawati Labenua
Keyword(s):  
Tissue Culture ◽  
Kappaphycus Alvarezii ◽  
Environmental Parameters ◽  
Culture Method ◽  
Salinity Treatment ◽  
Culture Methods ◽  
Culture Techniques ◽  
Randomized Design ◽  
Salinity Levels

HighlightThe best salinity for the thallus growth of K. alvarezii is 32 mg/LThe salinity indicates the osmotic balance of K. alvarezii seaweedThis osmoregulation process affects the nutrient absorption of K. alvarezii seaweedThis study aims to develop the availibility of superior seeds of K. alvareziiAbstract Kappaphycus alvarezii is one of the leading commodities in Indonesian waters. Demand for this commodity is quite high, as reflected in the increasing volume of exports each year. Fulfillment of these demands is obtained from the production of cultivation. Generally farmers get natural seaweed seedlings, namely cuttings from existing seaweed. The continuous use of seeds from nature can cause deterioration in the quality and quantity. Handling the problem of quality deterioration from seaweed seeds originating from nature, can be overcome by multiplying the seeds through tissue culture methods in vitro. In term of tissue culture techniques method, the most important thing to note is environmental parameters. An environment parameter that changes suddenly such as salinity can inhibit the growth of seaweed. Thus, this work is attampting the different salinity treatment on seaweed explants K. alvarezii. This study aims at determining (weight) the explants of K. alvarezii with a comparison of the different salinity levels in the in vitro tissue culture method. The method used in this study was a completely randomized design (CRD) with the different salinity treatments namely 30, 31, 32, 33, and 34 ppt. The results showed that the different salinities influenced the growth rate of K. alvarezii seaweed explants with the best explant growth at the salinity of 31 ppt, while the lowest growth value was obtained at 34 ppt

Toxin action b. Novyi (b. Oedematiens Weinberg-Sequin) on tissue growth in vitro and on cell metabolism. To the study of anaerobes and their toxin using the explantation method

1935 ◽  
Vol 31 (2) ◽  
pp. 271-272
Author(s):  
G. Barg
Keyword(s):  
Tissue Culture ◽  
Cell Metabolism ◽  
Anaerobic Bacteria ◽  
Culture Method ◽  
Tissue Growth ◽  
Tissue Culture Method ◽  
Series Of Experiments

The author has shown in a series of experiments that the tissue culture method is quite applicable for the study of anaerobic bacteria and their toxins, especially those of them that are significantly weakened by filtration.

scholarly journals Usage of the tissue culture method for the development of new forms of Fagopyrum esculentum Moench

2019 ◽  
pp. 3-6 ◽  
Author(s):  
E. N. Barsukova ◽  
A. G. Klykov ◽  
E. L. Chikina
Keyword(s):  
Heavy Metal ◽  
Tissue Culture ◽  
Copper Ions ◽  
Culture Method ◽  
Fagopyrum Esculentum ◽  
Tissue Culture Method ◽  
Fagopyrum Esculentum Moench ◽  
Effect Of Copper ◽  
Positive Effect

The article presents results of usage of the tissue culture method on buckwheat (Fagopyrum esculentum Moench) together with the selective factor increased concentrations of heavy metal ions in the nutrient medium. On the basis of Izumrud (Russia), Kitavase (Japan), hybrid Izumrud x Kitavase there were developed regenerant lines tolerant to copper and zinc. Negative action effect of copper ions was observed from the second generation in the second posterity of the plants-regenerants, developed from the callus, in the form of the chlorophyll-free mutants. Positive effect of the heavy metals was observed on increasing of rutin content of the plants-regenerants in vitro. Selective evaluation in the field conditions of the samples tolerant to heavy metal let to choose from them the most adaptive to monsoon conditions in Primorskykrai, which combine valuable traits of big grain (weight of 1000 grains 36-38 g), high seed productivity (1,9-3,2 g) and high rutin content in aboveground mass of plants (2,8-4,3 %).

scholarly journals Genotypic variability of Pinus sylvestris L. on the drought-resistance attribute

2019 ◽  
Vol 23 (1) ◽  
pp. 15-23
Author(s):  
E. Yu. Amineva ◽  
A. P. Gureev ◽  
T. M. Tabatskaya ◽  
O. S. Mashkina ◽  
V. N. Popov
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Drought Resistance ◽  
Culture Method ◽  
Callus Cultures ◽  
Seed Progeny ◽  
Tissue Culture Method ◽  
Drought Resistant ◽  
Resistant Genotypes

There was an increase and intensification of droughts in connection with global climate change in recent decades. Not all plant organisms are able to adapt to changing environmental conditions. Therefore, the question of stressresistant (drought-resistant) genotypes selection for breeding is quite urgent. This problem also concerns forest tree plants, including Scots pine, which is one of the main foresters in the Voronezh region. The results of vegetative and generative sphere analysis of individual Scotch pine trees with the help of biotechnology, molecular-genetic and cytogenetic method are given in this study. The possibility of applying the tissue culture method in vitro for testing initial plants for stress resistance, including drought resistance, is explained by the interconnection of cells, tissues and the whole plant properties. It is shown that the cytogenetic characteristics of the seed progeny and the indicators of callusogenic reactions do not always coincide in different genotypes: in some cases energy resources are spent on ontogeny protection, while, in other cases, to reproductive function maintain. There are trees, in which the state the generative sphere in arid years is at the level of optimal years, and their callus cultures reaction remains unchanged even under simulated conditions of drought. Based on the results obtained for the selection of Scots pine drought-resistant genotypes we suggest applying a system of criteria characterizing both the ability of the vegetative sphere to survive in drought conditions on the basis of the tissue culture method in vitro (callus tissue formation speed, its viability, frequency of callusogenesis), and the state of the generative sphere with the help of cytogenetic analysis of seed progeny (frequency of mitosis pathologies, the proportion of cells with micronuclei, mitotic activity). The expediency of applying the biotechnological approach was proved by analysis of the gene expression level of stress proteins: the level of AbaH gene expression correlates greatly with the indicator of the viability of callus cultures, including ones on a nutrient medium with an additional stress agent (NaCl). Trees that can be assigned to drought-resistant ones, according to the results of the analysis, should be recommended for use in breeding.

scholarly journals The Effect of Gibberellic Acid (GA3) On The In Vitro Seed Germination of Manggosteen (Garcinia mangostana)

el–Hayah ◽  
2019 ◽  
Vol 6 (4) ◽  
pp. 144-151
Author(s):  
Andini Andini ◽  
Riska Aqidatud Dzaroini ◽  
Ruri Siti Resmisari
Keyword(s):  
Slow Growth ◽  
Culture Method ◽  
Ms Medium ◽  
Garcinia Mangostana ◽  
Randomized Design ◽  
Tissue Culture Method ◽  
Long Time ◽  
Completely Randomized Design ◽  
Seeds Germination

Mangosteen is considered as fruit of many benefits with great popularity, rendering it valuable as an export commodity. Unfortunately, it has a relatively slow growth  and long early fruiting time. Generative propagation of mangosteen by seed result in low-yielding productionof qualified seedswith recalcitrant nature which means it cannot be stored for a long time. Whereas the result of vegetative propagation using grafting, branch cuttings and budding has difficulty rooting out. Tissue culture method requires growth regulators to control organogenesis and morphogenesis so it can accelerate the growth of the mangosteen explants. This study was arrangedintwo- factor completely randomized design (CRD). The observed variables werethe strength of the medium (½ and 1 MS) and GA3 concentrations (0 ppm; 0.25 ppm; 0.5 ppm; 0.75 ppm and 1 ppm). The variatonson GA3 concentration (0 ppm; 0.25 ppm; 0.5 ppm; 0.75 ppm; 1 ppm) on  different strength MS medium (½ MS and 1 MS) did not significantly affect the growth of radicle and sooth in the  in vitro seeds germination of Mangosteen (Garcinia mangostana).

scholarly journals 65 years of in vitro culture in Poland

2013 ◽  
Vol 82 (3) ◽  
pp. 183-192
Author(s):  
Maciej Zenkteler ◽  
Elżbieta Zenkteler
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Plant Disease Resistance ◽  
Culture Method ◽  
Short Review ◽  
Meristem Culture ◽  
Biological Processes ◽  
Practical Applications ◽  
Tissue Culture Method

This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements.

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