The Effect of Gibberellic Acid (GA3) On The In Vitro Seed Germination of Manggosteen (Garcinia mangostana)

Mangosteen is considered as fruit of many benefits with great popularity, rendering it valuable as an export commodity. Unfortunately, it has a relatively slow growth and long early fruiting time. Generative propagation of mangosteen by seed result in low-yielding productionof qualified seedswith recalcitrant nature which means it cannot be stored for a long time. Whereas the result of vegetative propagation using grafting, branch cuttings and budding has difficulty rooting out. Tissue culture method requires growth regulators to control organogenesis and morphogenesis so it can accelerate the growth of the mangosteen explants. This study was arrangedintwo- factor completely randomized design (CRD). The observed variables werethe strength of the medium (½ and 1 MS) and GA3 concentrations (0 ppm; 0.25 ppm; 0.5 ppm; 0.75 ppm and 1 ppm). The variatonson GA3 concentration (0 ppm; 0.25 ppm; 0.5 ppm; 0.75 ppm; 1 ppm) on different strength MS medium (½ MS and 1 MS) did not significantly affect the growth of radicle and sooth in the in vitro seeds germination of Mangosteen (Garcinia mangostana).

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Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana

E-Journal Menara Perkebunan ◽

10.22302/ppbbi.jur.mp.v74i2.103 ◽

2016 ◽

Vol 74 (2) ◽

Author(s):

Nurita TORUAN-MATHIUS ◽

. LUKMAN ◽

. AGUS-PURWITO

Keyword(s):

Tissue Culture ◽

Clonal Propagation ◽

Ms Medium ◽

Randomized Design ◽

Top Soil ◽

Completely Randomized Design ◽

Reduced Light ◽

In Vitro Micrografting ◽

Cinchona Ledgeriana

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization for Cinchona plantlets from micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of C. ledgeriana QRC clone were used as scions, while of C. succirubra as rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization. Acclimatization consisted of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC for two weeks, followed by aclimatization in a plastic house with 70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan. Pada tanaman kina teknik sambung mikro in vitro belum pernah dilakukan. Tujuan penelitian ini adalah menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup, jumlah daun, berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung mikro yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro. Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur suhu 25 - 27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini 90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal Cinchona sp..

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Optimization of in vitro rooting protocol for tomato (Lycopersicon esculentum mill.) varieties

Journal of Applied and Natural Science ◽

10.31018/jans.v12i3.2233 ◽

2020 ◽

Vol 12 (3) ◽

pp. 365-371

Author(s):

Endalkachew Baye ◽

Temesgen Matewos ◽

Derbew Belew

Keyword(s):

Lycopersicon Esculentum ◽

Root Length ◽

In Vitro Rooting ◽

Root Induction ◽

Ms Medium ◽

Lycopersicon Esculentum Mill ◽

Randomized Design ◽

Optimized Protocol ◽

Completely Randomized Design

In vitro rooting of micropropagated shoots were carried out with the aim of evaluating the root induction responses of two tomatoes (Lycopersicon esculentum MILL) varieties (Gelilema and Chali) using Indole -3- butyric acid (IBA). Seven levels of IBA (0.0, 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5 mg L-1) were used in a completely randomized design (CRD) in factorial combinations (seven level of IBA*two varieties) with three replications. After the plantlets were kept in the rooting media for three weeks, data on rooting percentage, number of roots/shoots and root length in cm were collected. The analysis of variance showed that the interaction of IBA*Var was highly significantly different for rooting percentage, a number of roots/shoot and root length at p<0.01. The highest rooting percentage (100.00±0.00), number of roots/shoot (14.20±0.35) and root length (10.7±0.29) were received from Chali on free Murashige and Skoog medium (MS). At the same time, the lowest percentage of rooting (11.11±0.00), number of roots/shoot (0.887±0.19) and root length (1.00±0.00 cm) were obtained from Gelilema on MS+1.5mg/l IBA. For acclimatization, the in vitro rooted shoots were transplanted into plastic pots containing a mixture of oven sterilized soil and sand at a ratio of 2:1. After three weeks, a survival rate of 67.7% for Chali and 58.1% for Gelilema was obtained. From the above result, it can be concluded that free MS medium was the best for in vitro rooting of the two tomato varieties. The optimized protocol will be useful for rapid in vitro multiplication of the two tomato varieties.

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The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f100204 ◽

2012 ◽

Vol 10 (2) ◽

pp. 54-60

Author(s):

IRMA PUTRI HAYANTI ◽

RETNA BANDRIYATI AMIPUTRI ◽

PRASWANTO PRASWANTO

Keyword(s):

Shoot Growth ◽

Organic Fertilizer ◽

Centella Asiatica ◽

Culture Method ◽

Shoot Formation ◽

Randomized Design ◽

Leaf Formation ◽

Medicinal Crop ◽

Completely Randomized Design

Hayanti IP, Amiputri RB, Praswanto. 2012. The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro. Biofarmasi 10: 54-60. Gotu kola (Centella asiatica L.) is a medicinal crop, needed by local industry of pharmacy. Gotu kola in Indonesia is not enough to supply in market requisites. Tissue culture method was used to multiply gotu kola with a combination of medium from liquid organic fertilizer and pearl grass extract. The purposes of the research were to determine the effect of liquid organic fertilizer and pearl grass extract on the shoot growth of gotu kola in vitro, and to obtain the optimal concentration of liquid organic fertilizer and pearl grass extract combination on the shoot formation of gotu kola in vitro. The research was conducted in November 2010 to June 2011 in Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, Surakarta. The experimental design used was a Completely Randomized Design (CRD) with two treatment factors and three replications. The first factor was liquid organic fertilizer concentrations, i.e. 0 mL/L, 2 mL/L, 4 mL/L and 8 mL/L. The second factor was pearl grass extract, i.e. 0 mL/L, 3 mL/L, 6 mL/L and 12 mL/L. Variables observed were the percentage of shoot formation, the time of shoot formation, the number of shoot, the length of shoot, the percentage of leaf formation, the time of leaf formation and the number of leaf. The result of the research showed that not all of treatments provided shoot formation. The combination of liquid organic fertilizer concentrations 0 mL/L and pearl grass extract 12 mL/L showed the best results, in which the average of length of shoot was 2.16 cm, the number of shoot was 8 and the number of leaf was 8.

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Effect of salinity and drought stress on morphological and biochemical properties of two Iranian fenugreek (Trigonella foenum-graecum) populations

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha49212038 ◽

2021 ◽

Vol 49 (2) ◽

pp. 12038

Author(s):

Afsaneh TAVANGAR ◽

Leila KARAMI ◽

Mohammad HEDAYAT ◽

Gholamreza ABDI

Keyword(s):

Drought Stress ◽

Biochemical Properties ◽

Ms Medium ◽

Randomized Design ◽

Terminal Bud ◽

Micro Propagation ◽

Catalase Peroxidase ◽

Completely Randomized Design ◽

Callus Volume

In this study, micro propagation of two Iranian fenugreek populations and their morphological and biochemical responses to salinity and drought stresses in in vitro culture condition were conducted using factorial experiment in a completely randomized design in three replications. Different explant type (terminal bud, cotyledon and epicotyledon explant) were cultured in MS medium contain different concentration of plant growth regulators such as kin (0, 0.5 and 1 mg / l) and 2,4-D (0.5, 1 and 2 mg / l). Murashige and Skoog (MS) medium supplemented with 1 mg/l kinetin and 2 mg/l 2,4-D showed the highest callus proliferation rate per explants in both populations. The highest amount of callus volume was obtained from the explants of the terminal bud. Proliferated calli from terminal bud explant were green and yellowish, from cotyledon were yellowish to white with soft texture, and the cotyledons were greenish and compact. The results of salinity stresses with different concentrations of sodium chloride (0, 70 and 120 mM) and drought stress with polyethylene glycol (0, 5 and 10%) showed that both stresses decreased callus growth and increased total protein, proline, catalase, peroxidase and trigonelin content in both populations. Trigonelin measurement showed that ‘Borazjan’ papulation had higher trigonelin content, in vitro, than ‘Ardestan’ papulation.

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Production of reserpine of rauwolfia serpentina [L] kurz ex benth through in vitro culture enriched with plant growth regulators of NAA and kinetin

International Journal of Engineering & Technology ◽

10.14419/ijet.v7i2.29.13331 ◽

2018 ◽

Vol 7 (2.29) ◽

pp. 274 ◽

Cited By ~ 1

Author(s):

Muhammad Subandi ◽

Dikayan . ◽

Efrin Firmansyah

Keyword(s):

In Vitro Culture ◽

Growth Regulators ◽

High Performance ◽

Culture Method ◽

Naphthalene Acetic Acid ◽

Randomized Design ◽

Completely Randomized Design ◽

Institute Of Technology ◽

Design Weight

Rauwolfia serpentine [L] Kurz ex Benth is a valuable plant yielding alkaloid of reserpine. The aim of this study was to find the most effective way of reserpine production. Growth regulators {naphthalene acetic acid [NAA] and Kinetin}were applied to promote and to regulate the growth of explant of R. serpentine cultured in vitro. The study was conducted at the laboratory of Bandung Institute of Technology [ITB]. Nine formulations of NAA and Kinetin were the treatments that were repeated twice in completely randomized design. Weight of callus was measured at one, two, three and four weeks after induction of explant. Reserpine analysis was performed by High Performance Liquid Chromatography [HPLC]. The result showed that the best callus induction was the treatment of 2.5 ppm NAA + 2.5 ppm Kinetin, and the highest content of reserpine was in root organ [0.0021 g / L], and in callus [0.0021 g / L] at the age of 4 weeks after induction. There was revealed that in vitro culture method was more productive in producing reserpine compound than the conventional plantation of R serpentine. Production of reserpine by callus culture was more effective and may be the basic for recommended further effort.

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INDUKSI KALUS CENGKEH DARI EKSPAN DAUN MENGGUNAKAN 2,4-D SECARA IN VITRO

J-PEN Borneo : Jurnal Ilmu Pertanian ◽

10.35334/jpen.v2i3.1533 ◽

2020 ◽

Vol 2 (2) ◽

Author(s):

Yulianti Rasud ◽

Zainuddin Basri ◽

Nirwan Sahiri

Keyword(s):

Growth Regulators ◽

Callus Induction ◽

Callus Formation ◽

Cell Mass ◽

Medium Composition ◽

Ms Medium ◽

Plant Parts ◽

Randomized Design ◽

Completely Randomized Design

ABSTRACT Callus induction is one method of tissue culture which is done by stimulating cell division continuously from certain plant parts such as leaves, roots, stems, and so on by using growth regulators to form cell mass. The cell mass (callus) will then regenerate through organogenesis or embryogenesis to become a new plant. One of the growth regulators used for callus induction is 2,4-D. The aims of this experiments was to evaluate the best concentration of 2,4-D for callus induction of clove leaves. The experiment used Completely Randomized Design with treatment tested was concentrations of 2,4-D, consisted of six levels, namely 0.5 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm, 2.5 ppm and 3.0 ppm. Results of this experiments indicated that the best medium composition for callus induction was MS medium supplemented with 0.5 ppm 2,4-D. In the medium composition, the fastest callus formation, namely 6.00 weeks after culture and the percentage of callus formation reached 100% with the color and texture of the resulting callus white and crumb. Keyword : Callus Induction, Clove, 2,4-DABSTRAK Induksi kalus merupakan salah satu metode kultur jaringan yang dilakukan dengan jalan memacu pembelahan sel secara terus menerus dari bagian tanaman tertentu seperti daun, akar, batang, dan sebagainya dengan menggunakan zat pengatur tumbuh hingga terbentuk massa sel. Massa sel (kalus) tersebut selanjutnya akan beregenerasi melalui organogenesis ataupun embriogenesis hingga menjadi tanaman baru. Salah satu zat pengatur tumbuh yang digunakan untuk induksi kalus adalah 2,4-D. Penelitian ini bertujuan menentukan konsentrasi 2,4-D yang lebih baik untuk induksi kalus daun cengkeh. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Media dasar yang digunakan adalah media MS yang ditambahkan berbagai konsentrasi 2,4-D yaitu 0,50 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, dan 3 ppm. Hasil penelitian menunjukkan bahwa komposisi media yang terbaik untuk induksi kalus daun cengkeh adalah media MS yang ditambahkan 0,5 ppm 2,4-D. Pada komposisi media tersebut diperoleh saat muncul kalus paling cepat, yaitu rata-rata 6,00 MST dengan persentase pembentukan kalus tertinggi mencapai 100% dengan warna dan tekstur kalus yang dihasilkan putih dan remah. Kata Kunci : Induksi Kalus, Cengkeh, 2,4-D.

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Efikasi Beberapa Agens Hayati Terhadap Penekanan Pertumbuhan Pyricularia grisea Secara In Vitro

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v23i1.48174 ◽

2021 ◽

Vol 23 (1) ◽

pp. 31

Author(s):

Shyntiya Ayu Lestari ◽

Umi Kalsum ◽

Evan Purnama Ramdan

Keyword(s):

Negative Impact ◽

Culture Method ◽

Biological Agents ◽

Biological Agent ◽

Blast Disease ◽

Pyricularia Grisea ◽

Randomized Design ◽

Completely Randomized Design ◽

Chemical Pesticides

The demand for rice as an important food crop in Indonesia is still constrained by the attack of blast disease caused by Pyricularia grisea. Controlling using synthetic chemical pesticides has a negative impact on the environment so that the use of biological agents is an alternative option. This study aims to determine the effectiveness of several biological agents against the pathogen P. grisea that causes blast disease in rice plants in vitro. The study used a completely randomized design (CRD) with 6 levels of treatment and was repeated 3 times using the dooble culture method. The results of the study showed that the inhibition of P. grisea was the highest in the treatment of P. grisea × Trichoderma sp. and P. grisea × Gliocladium sp. namely 67.04% and 51.85% compared to other treatments. The P. polymyxa and P. fluorescence treatments showed low inhibition, namely 23.70% and 28.89%. Biological agents Trichoderma sp. and Gliocladium sp. able to inhibit the growth of the fungus P. grisea. Each biological agent has a different percentage of inhibition in that caused by the inhibitory mechanism of the biological agent.

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PERTUMBUHAN PLANLET KRISAN (Dendranthema grandiflora Tzvelev) KULTIVAR PINK FIJI SETELAH PENAMBAHAN EKSTRAK TAUGE (Vigna radiata L.) PADA MEDIUM MURASHIGE DANSKOOG (MS) SECARA IN VITRO.

Jurnal Ilmiah Biologi dan Keanekaragaman Hayati ◽

10.23960/jbekh.v5i2.50 ◽

2019 ◽

Vol 5 (2) ◽

pp. 36-41

Author(s):

Nalindri Impitasari ◽

Endang Nurcahyani ◽

Tundjung Tripeni Handayani ◽

Yulianty Yulianty

Keyword(s):

Vigna Radiata ◽

Economic Value ◽

Ornamental Plants ◽

Natural Sciences ◽

Ms Medium ◽

Dendranthema Grandiflora ◽

Community Interest ◽

Randomized Design ◽

Completely Randomized Design

Chrysanthemum (Dendranthema grandiflora Tzvelev) is one of the important ornamental plants in Indonesia and has high economic value . This plant is known as a producer of flowers with attractive shapes and colors . Seeing the magnitude of community interest and the potential utilization of chrysanthemum , causing this plant more and more developed and cultivated . This study aims to determine the concentration of optimum mungbean sprouts extract on the growth of chrysanthemum explants in vita . The addition of mungbean sprouts extracts (Vigna radiata L .) from concentration of 0% v/v , 2% v/v , 4% v/v , 6% v/v and 8% v/v on Murashige and Skoog(MS ) medium to growth eksplan Chrysanthemum (Dendranthema grandiflora Tzvelev ) Pink Fiji cultivars have been carried out in the tissue culture laboratory of Faculty of Mathematics and Natural Sciences , University of Lampung from November to December 2017 . This study used Completely Randomized Design (RAL ) 1 factor with 5 replications . Analysis of BNT variety and test is done at 5% level . The results showed that the extract from mungbean sprouts (Vigna radiata L . ) had no effect on plantlet height , number of shoot and number of chrysanthemum (Dendranthema grandiflora Tzvelev) plantlet leaves. The addition of mungbean spourts extracts on Murashige and skoog (MS) medium show 100% live plantlet.

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Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro

Paspalum: Jurnal Ilmiah Pertanian ◽

10.35138/paspalum.v7i1.107 ◽

2019 ◽

Vol 7 (1) ◽

pp. 16

Author(s):

Ujang Siron ◽

Noertjahyani Noertjahyani ◽

Yana Taryana ◽

Romiyadi Romiyadi

Keyword(s):

Research Method ◽

Dry Weight ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Medium Culture

The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium spectabile orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1. The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves. Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets. BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture

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PENGEMBANGAN BIBIT UNGGUL PORANG (Amarphopallus oncophilus) MELALUI TEKNIK KULTUR IN VITRO UNTUK MENDUKUNG KETAHANAN PANGAN NASIONAL

el–Hayah ◽

10.18860/elha.v3i1.2216 ◽

2001 ◽

Vol 3 (1) ◽

Author(s):

Dwi Suheriyanto ◽

Romaidi Romaidi ◽

Ruri Siti Resmisari

Keyword(s):

Mortality Rate ◽

Harvest Index ◽

Medium Effect ◽

Ms Medium ◽

Plant Propagation ◽

Randomized Design ◽

Young Leaves ◽

Number Of Leaves ◽

Completely Randomized Design

Porang (Amarphophallus. oncophyllus) is a type of plant tubers that have the potential and prospects for development in Indonesia. In addition to readily available, plants are also capable of producing carbohydrates and high harvest index. The last few years a very large porang needs. Porang plant propagation is vegetative and generative in which to make the seeds are ready to harvest takes between 4-6 months. This study aims to determine kinds of the appropriate medium for growing plants porang network and determine the concentration of each medium, so we get a superior porang plants. Research using factorial completely randomized design. The first factor is a growth regulator BAP concentration: 0 mg / l, 0.5 mg / l, 1.0 mg / l, 1.5 mg / l and 2.0 mg / l, while the second factor is the concentration of IBA 0 mg / l, 0.5 mg / l and 1.0 mg / l, in order to obtain 15 treatments and each treatment made 15 replications. Data were collected for the pengkalusan, contamination callus, callus mortality rate, number of buds, leaf buds height, number of leaves and root. The results showed that the addition of plant growth regulators (hormones) BAP in MS medium effect on the number of shoots, buds and the high number of young leaves. The addition of hormone IBA in MS medium pengkalusan affect the process and the number of roots. The addition of hormone BAP and IBA on MS medium did not significantly affect mortality contamination callus and callus. The interaction between BAP and IBA hormone given on MS medium did not significantly affect the pengkalusan, contamination callus, callus mortality rate, number of buds, leaf buds height, number of leaves and root

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