scholarly journals SNPs detection in 5’-UTR region of the MC4R gene in Garut sheep

2021 ◽  
Vol 888 (1) ◽  
pp. 012001
Author(s):  
A P Z N L Sari ◽  
I R Athifa ◽  
Panjono ◽  
R Hidayat ◽  
A Barli ◽  
...  
Keyword(s):  
Growth Traits ◽  
Equilibrium Analysis ◽  
Sample Population ◽  
Alignment Position ◽  
Blood Samples ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
Genotype Frequencies ◽  
Hardy Weinberg Equilibrium ◽  
G 14

Abstract The melanocortin-4 receptor (MC4R) gene has been linked to controlling feeding behavior and body weight. The objective of this study was to detect the polymorphism within the 5’-UTR region of the MC4R gene in Garut sheep. A total of 36 blood samples were isolated and amplified using primers (forward: 5’-TTCGTTTGGGGCAAGTCAAG-3’ and reverse: 5’-GGAAACGCTCACCAACATGT-3’). Two SNPs, g.396C/T and g.399C/G, were discovered in the 5‘UTR region based on sequence alignment (position number based on Genbank acc no. NC_040274). For both SNPs, only two genotypes were found in the samples. Both SNPs had identical allele and genotype frequencies. The C allele (86%, n=26) was higher than the T (g.396C/T) and G allele (g.399C/G) (14%, n=10). The homozygous CC genotype has a higher frequency (72%) in both SNP g.396C/T and g.399C/G, followed by CT and CG genotype (28%), respectively. The Hardy-Weinberg equilibrium analysis resulted in the sample population did not deviate (χ2<5.59). Further analysis could be suggested to provide an overview of this polymorphism effect in Garut sheep’s growth traits.

scholarly journals Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat

2018 ◽  
Vol 43 (4) ◽  
pp. 343 ◽  
Author(s):  
L. Latifah ◽  
D. Maharani ◽  
A. Kustantinah ◽  
T. Hartatik
Keyword(s):  
Growth Traits ◽  
Receptor Gene ◽  
Marker Gene ◽  
Average Daily Gain ◽  
Mc4r Gene ◽  
Relationship Analysis ◽  
Melanocortin 4 Receptor ◽  
Genotype Frequencies ◽  
Potential Marker ◽  
Melanocortin 4 Receptor Gene

Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5’-TCGGGCGTCTTGTTCATCAT-3’ and R: 5’-CAAGACTGGGCACTGCTTCA-3’). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat.

Association study of rs17782313 polymorphism near MC4R gene with obesity/overweight, BMI, and hedonic hunger among women from Northwestern Iran

2021 ◽  
pp. 1-12
Author(s):  
Mahan Narjabadifam ◽  
Morteza Bonyadi ◽  
Seyed Abbas Rafat ◽  
Reza Mahdavi ◽  
Fereshteh Aliasghari
Keyword(s):  
Medical Condition ◽  
Sequencing Data ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
Group Methods ◽  
Pcr Rflp ◽  
Hedonic Hunger ◽  
Hardy Weinberg Equilibrium ◽  
Northwestern Iran ◽  
Different Populations

BACKGROUND: Obesity, as a medical condition, results from interactions between environmental and genetic factors. The rs17782313 polymorphism, located 188kb downstream of the Melanocortin 4 Receptor (MC4R) gene, is one of the essential candidate genetic markers that has shown the highest association with obesity in different populations. OBJECTIVE: This study aimed to investigate the possible associations of rs17782313 polymorphism near the MC4R gene with obesity/overweight, body mass index (BMI), and hedonic hunger among women from the Iranian Azeri ethnic group. METHODS: Five hundred sixty-three women, composed of 396 patients with obesity/overweight and 167 unrelated healthy controls, were genotyped for the rs17782313 polymorphism by applying the polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) method. RESULTS: This population was in Hardy-Weinberg equilibrium (P = 0.878). The study confirmed a significant association of rs17782313 with obesity, where subjects carrying the C/C genotype had higher odds of obesity (OR = 2.681, P = 0.005, 95%CI:1.340–5.365). Also, C allele carriers have statistically significantly higher BMI scores than those carrying the T allele (P = 0.029). However, no significant associations were found among PFS scores and genotypic/allelic groups of rs17782313 polymorphism (P = 0.368). CONCLUSIONS: Our findings suggest that rs17782313 polymorphism is strongly associated with obesity and BMI but not with hedonic hunger among Northwest Iran women. Moreover, the sequencing data analysis in several homozygous and heterozygous carriers of the C allele led to identifying a novel frameshift variant with TCT deletion (rs534212081) in the 166 upstream of rs17782313, which has not been reported so far.

scholarly journals Melanocortin-4 receptor and leptin as genes for the selection of superior Madrasin cattle

2021 ◽  
pp. 3224-3228
Author(s):  
Budi Utomo ◽  
Rimayanti Rimayanti ◽  
Indah Norma Triana ◽  
Amaq Fadholly
Keyword(s):  
Genetic Improvement ◽  
Fragment Length Polymorphism ◽  
Growth Traits ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
Polymerase Chain ◽  
Selection Of ◽  
Restriction Fragment Length

Background and Aim: The genetic improvement of cattle through livestock section is based on quantitative, qualitative, and molecular characteristics. This study examined polymorphisms of the melanocortin-4 receptor (MC4R) and leptin genes as a reference for the selection of superior breeds in Madrasin cattle. Materials and Methods: The leptin and MC4R genes of Madrasin cattle were amplified using polymerase chain reaction (PCR); then, restriction fragment length polymorphism of the leptin gene was performed using the restriction enzyme BsaA1, at site 2793 with ACGT point position. Results: The leptin gene was divided into three bands, namely, AA with one fragment (522 bp), CG with two fragments (441 bp and 81 bp), and AG with three fragments (522 bp, 441 bp, and 81 bp). The MCR-4 gene was divided into three bands, namely, 493 bp, 318 bp, and 175 bp. Conclusion: The MC4R and leptin genes can act as molecular markers for growth traits in Madrasin cattle and can be used to genetically optimize and improve growth. The GG allele of the MC4R gene and the AA allele of the leptin gene can be used in Madrasin cattle.

scholarly journals Melanocortin-4 Receptor (MC4R) gene polymorphism and its effect on growth traits in Madura cattle

2019 ◽  
Vol 44 (1) ◽  
pp. 38
Author(s):  
P. W. Prihandini ◽  
S. Sumadi ◽  
G. Suparta ◽  
D. Maharani
Keyword(s):  
Growth Traits ◽  
Balance Control ◽  
Direct Sequencing ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
Pcr Rflp ◽  
Reverse Primer ◽  
Selection For

Melanocortin-4 receptor (MC4R) gene has an important role in the regulation of feed intake and energy balance control. The objective of this study was to identify the single nucleotide polymorphisms (SNPs) of MC4R gene and their association with growth traits in Madura cattle. A total of 198 calves were used in this study.Forward primer: 5’-GTCGGGCGTCTTGTTCATC-3’and reverse primer: 5’-GCTTGTGTTTAGCATCGCGT-3’ were used to amplify approximately 493 bp of MC4R gene. The results showed that two SNPs, g.1133C>G and g.1108C>T were identified by direct sequencing. The PCR-RFLP method was performed to genotype all individuals studied based on SNP g.1133C>G, and its SNP was significantly associated with shoulder height (SH) at yearling age (P<0.05). Animals with GG genotype had a higher SH (110.35±6.40cm) than those with CC (102.00±8.00 cm) and CG genotype (105.96±6.23 cm). The SNP g.1133 C>G changed amino acid from valine to leucine. In conclusion, the SNP g.1133C>G of the MC4R gene may be used as a marker-assisted selection for SH trait in Madura cattle.

scholarly journals Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

2018 ◽  
Vol 43 (2) ◽  
pp. 87
Author(s):  
D. Maharani ◽  
A. Fathoni ◽  
S. Sumadi ◽  
T. Hartatik ◽  
M. Khusnudin
Keyword(s):  
Body Length ◽  
Growth Traits ◽  
Average Daily Gain ◽  
Pcr Amplification ◽  
Growth Trait ◽  
Chest Circumference ◽  
Nucleotide Polymorphisms ◽  
Chi Square ◽  
Mc4r Gene ◽  
Genotype Frequencies

MC4R gene is known as an important candidate gene for the growth trait. The purpose of this research was to identify the MC4R gene in Kebumen Ongole grade cattle and examine its association with growth traits. Data of birth weight (BW), weaning weight (WW), birth body length (BBL), birth chest circumference (BCC), birth shoulder height (BSH), weaning body length (WBL), weaning chest circumference(WCC), weaning shoulder height (WSH) and average daily gain (ADG) were collected and used for analysis of MC4R gene. Sixty blood samples were collected for DNA isolation and PCR amplification. The single nucleotide polymorphisms (SNP) g.1133 C>G was used for genotyping by using PCR-RFLP methods. The frequenciy of G allele (0.59) was greater than C allele (0.41). The highest genotype frequencies have been detected in CG heterozygote animals (0.52) followed by GG (0.33) and CC (0.15) in homozygote animals. The results of Pearson ‘s Chi-square test indicated that the population was not deviate (P>0.05) from the Hardy-Weinberg equilibrium (HWE). The SNP g. 1133 C>G of MC4R gene indicated affecting high birth body length with GG genotype (P<0.05). In conclusion, the SNP g. 1133 C>G may can be a marker for birth body length of calf selection.

scholarly journals Association of the melanocortin 4 receptor (MC4R) gene polymorphism with growth traits of Hu sheep

2020 ◽  
Vol 192 ◽  
pp. 106206
Author(s):  
Huili Shan ◽  
Xuemei Song ◽  
Yuhao Cao ◽  
Pei Xiong ◽  
Jianliang Wu ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Growth Traits ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
Hu Sheep

scholarly journals Association of a missense mutation of the <i>MC4R</i> gene with growth traits in cattle (Brief report) (Assoziation einer Mutation im MC41 Gen mit Wachstumsmerkmalen beim Rind)

2006 ◽  
Vol 49 (5) ◽  
pp. 515-516
Author(s):  
C. L. Zhang ◽  
H. Chen ◽  
Y. H. Wang ◽  
X. Y. Lan ◽  
L. Zhang ◽  
...  
Keyword(s):  
Missense Mutation ◽  
Energy Homeostasis ◽  
Growth Traits ◽  
G Protein Coupled Receptors ◽  
Ingestive Behavior ◽  
Coding Region ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
G Protein Coupled ◽  
Human And Mouse

Abstract. Melanocortin-4 receptor (MC4R) is one of five G-protein-coupled receptors binding melanocortins that is implicated in the control of ingestive behavior and energy homeostasis. Mutations have been described in the human and mouse MC4R genes which are associated with obesity (YEO et al., 1998; HUSZAR et al., 1997). Moreover, a mutation in porcine MC4R is associated with economically important traits in the pig (KIM et al., 2000). The SNPs reported in bovine MC4R coding region were specific to breeds (VALLE et al., 2004; THUE et al., 2001; HAEGEMAN et al., 2001). In the present experiment over 95% of the coding region of MC4R were screened to detect the SNPs in the predominant cattle breeds of China. Association of a missense mutation of MC4R gene with growth traits was analyzed.

scholarly journals Melanocortin-4 Receptor (MC4R) Gene as the Main Gene for Rapid Growth Selection in Beef Cattle

2019 ◽  
Vol 29 (2) ◽  
pp. 85 ◽  
Author(s):  
Peni Wahyu Prihandini ◽  
D Maharani
Keyword(s):  
Beef Cattle ◽  
Candidate Gene ◽  
Rapid Growth ◽  
Genetic Improvement ◽  
Growth Traits ◽  
Economic Traits ◽  
Improvement Program ◽  
Selection Program ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor

Genetic improvement for economic traits such as growth is important in beef cattle selection program. This paper discusses melanocortin-4 receptor (MC4R) gene as a candidate gene for rapid growth based on its function, location and pathway, as well as its polymorphism and identified genotypes associated with economic traits, and its application as a marker-assisted selection. MC4R is a prominent gene encoding growth traits and has a critical role in mediating the effect of leptin in regulating food consumption and energy balance. Many identified MC4R gene polymorphisms are associated with growth traits. The MC4R gene, therefore, is considered as a functional candidate gene for growth traits and can be used as a marker in selection program based on molecular genetics. The use of molecular markers such as MC4R gene, therefore, can be applied in genetic improvement program for growth traits in cattle. By using MC4R gene, the efforts to build breeding system in small populations can be proposed. For instance, with an effective population size (Ne) of about 40 heads, the first generation (G1) will be obtained with a composition of GG 61.1%, CG 33.3% and CC 5.6% and male and female ratio of 178:11 and generates ideal Ne of 41 heads. In the third generation, therefore, GG composition will be 100%. The results of molecular analysis can be further used as a guideline in the development and genetic improvement strategies of beef cattle.

scholarly journals Polymorphism of gene glutathion-s-transferase Pi in teenagers from Buryat ethnic group living in the irkutsk region

2016 ◽  
Vol 1 (5) ◽  
pp. 165-169
Author(s):  
Беляева ◽  
Elena Belyaeva ◽  
Ершова ◽  
Oksana Ershova
Keyword(s):  
Ethnic Group ◽  
Group Living ◽  
Chi Square ◽  
Blood Samples ◽  
Weinberg Equilibrium ◽  
Genotype Frequencies ◽  
Irkutsk Region ◽  
Significant Difference ◽  
Hardy Weinberg Equilibrium ◽  
Gstp1 Gene

Human glutathione-S-transferases play an important role in phase II detoxification process. But poly-morphism in the GSTP1 gene has not been studied in certain populations.Aim: to determine the distribution of allele and genotype frequencies of GSTP1 gene in teenagers from Buryat ethnic group.Materials and methods. Blood samples were obtained from 55 teenagers from Buryat ethnic group. There were 28 males and 27 females with an age of 14.05±0.99 years over the range of 13 to 16. DNA was isolated from blood samples. The polymerase chain reaction was used to amplify A313G and C341T markers of the GSTP1 gene. Chi-square testing was used to evaluate the significant difference of the GSTP1 genotype frequencies between observed and expected values.Results. Allele and genotype frequencies of A313G and C341T markers GSTP1 were determined in teenagers from Buryat ethnic group. The study showed that the frequencies of A and G alleles at the A313G marker were 0.809 and 0.191 while those of C and T alleles at the C341T marker were 0.973 and 0.027, respectively. The distribution of the genotype frequencies at the A313G marker were consistent with expected in a Hardy–Weinberg equilibrium (χ2=0 .77; d.f.=1; p&#62;0.05). However, the distribution of the genotype frequencies at the C341T marker were not consistent with expected in a Hardy – Weinberg equilibrium (χ2= 0.043; d.f.= 1; p&#60;0.05). It was because the homozygous of T allele was not found in the ethnic group of Buryat.

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