scholarly journals Specialized Cell-Free DNA Blood Collection Tubes Can Be Repurposed for Extracellular Vesicle Isolation: A Pilot Study

2020 ◽  
Vol 18 (5) ◽  
pp. 462-470 ◽  
Author(s):  
Jessica Heatlie ◽  
Vanessa Chang ◽  
Sandra Fitzgerald ◽  
Yohanes Nursalim ◽  
Kate Parker ◽  
...  
Keyword(s):  
Pilot Study ◽  
Extracellular Vesicle ◽  
Blood Collection ◽  
Cell Free Dna ◽  
Specialized Cell ◽  
Free Dna ◽  
Blood Collection Tubes

scholarly journals Comparison of Blood Collection Tubes from Three Different Manufacturers for the Collection of Cell-Free DNA for Liquid Biopsy Mutation Testing

2017 ◽  
Vol 19 (5) ◽  
pp. 801-804 ◽  
Author(s):  
Christina Alidousty ◽  
Danielle Brandes ◽  
Carina Heydt ◽  
Svenja Wagener ◽  
Maike Wittersheim ◽  
...  
Keyword(s):  
Liquid Biopsy ◽  
Mutation Testing ◽  
Blood Collection ◽  
Cell Free Dna ◽  
Free Dna ◽  
Blood Collection Tubes

Abstract 3110: In depth comparison of cell free DNA blood collection tubes

2020 ◽  
Author(s):  
Joel Desharnais ◽  
Jacob M. Vasquez ◽  
Katya J. Reshatoff ◽  
Quyen Bui ◽  
Han-Mei Chen ◽  
...  
Keyword(s):  
Blood Collection ◽  
Cell Free Dna ◽  
Free Dna ◽  
Blood Collection Tubes

Zero delay plasma vs. Streck cfDNA tubes: Does immediately centrifuging blood at the point of draw improve cell-free DNA signal-to-noise?

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e14529-e14529
Author(s):  
Greg Sommer ◽  
Laura Fredriksen ◽  
Gabriella Iacovetti ◽  
Kyungjin Hong ◽  
Ulrich Schaff
Keyword(s):  
Room Temperature ◽  
Blood Collection ◽  
Signal To Noise ◽  
Blood Draw ◽  
Plasma System ◽  
Cell Free Dna ◽  
Clinical Evaluations ◽  
Free Dna ◽  
Collection Tube ◽  
Blood Collection Tubes

e14529 Background: Sample quality is a critical consideration for high fidelity cell-free DNA (cfDNA) testing. Oncological cfDNA tests used for liquid biopsy typically employ specialty blood collection tubes containing chemical preservatives to minimize degradation of samples prior to lab testing. Here we describe a newly developed device, Zero Delay Plasma– a portable centrifuge and disc system designed to immediately isolate cell-free plasma at the point of blood draw – and evaluate its performance against the Streck cfDNA collection tube. Methods: Whole blood was collected, processed, and stored at room temperature for up to 7 days with both the Zero Delay Plasma system and the Streck cfDNA blood collection tube. Sample hemolysis was measured via cell-free hemoglobin. Genomic contamination and cfDNA signal-to-noise were evaluated by qPCR and electrophoresis, comparing signal from target 150-200bp cfDNA to contaminating longer length genomic sequences in the sample. 2 sets of hemolysis experiments, 2 sets of electrophoresis experiments and 4 sets of qPCR experiments were conducted. Results: Plasma processed with the Zero Delay Plasma system yielded ~4X lower hemolysis levels, ~10X lower genomic contamination, and ~20X higher cfDNA signal-to-noise compared to the Streck cfDNA collection tube after 7 days of storage at room temperature. Conclusions: The Zero Delay Plasma system minimizes sample degradation and analytical background signal for cfDNA testing by immediately removing cells and other contaminants at the point of blood collection. Clinical evaluations are in process.

scholarly journals Cell-Free DNA Blood Collection Tubes Are Appropriate for Clinical Proteomics: A Demonstration in Colorectal Cancer

2018 ◽  
Vol 12 (3) ◽  
pp. 1700121 ◽  
Author(s):  
Juhura G. Almazi ◽  
Peter Pockney ◽  
Craig Gedye ◽  
Nathan D. Smith ◽  
Hubert Hondermarck ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Clinical Proteomics ◽  
Blood Collection ◽  
Cell Free Dna ◽  
Free Dna ◽  
Blood Collection Tubes

scholarly journals Efficient Detection of BRAF Mutation in Plasma of Patients after Long-term Storage of Blood in Cell-Free DNA Blood Collection Tubes

2015 ◽  
Vol 61 (6) ◽  
pp. 886-888 ◽  
Author(s):  
Marc G Denis ◽  
Anne-Chantal Knol ◽  
Sandrine Théoleyre ◽  
Audrey Vallée ◽  
Brigitte Dréno
Keyword(s):  
Braf Mutation ◽  
Blood Collection ◽  
Cell Free Dna ◽  
Long Term Storage ◽  
Free Dna ◽  
Efficient Detection ◽  
Blood Collection Tubes ◽  
Term Storage

Abstract 3145: Clinical evaluation of streck cell-free DNA blood collection tubes for liquid profiling in oncology

2016 ◽  
Author(s):  
Inga Medina Diaz ◽  
Stefan Holdenrieder ◽  
Annette Nocon ◽  
Makbule Kobilay ◽  
Dirk Skowasch ◽  
...  
Keyword(s):  
Clinical Evaluation ◽  
Blood Collection ◽  
Cell Free Dna ◽  
Free Dna ◽  
Blood Collection Tubes

Abstract 5683: Evaluation of the utilization of blood collection tubes for cell-free DNA research

2017 ◽  
Author(s):  
Fumitaka Endo ◽  
Takeshi Iwaya ◽  
Takehiro Chiba ◽  
Mizunori Yaegashi ◽  
Kei Sato ◽  
...  
Keyword(s):  
Blood Collection ◽  
Cell Free Dna ◽  
Free Dna ◽  
Blood Collection Tubes

scholarly journals Hemolysis and Fetal Fraction in Cell-Free DNA Blood Collection Tubes for Noninvasive Prenatal Testing

2020 ◽  
Vol 24 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Renee Stokowski ◽  
Karen White ◽  
Coleen Hacker ◽  
Jigna Doshi ◽  
Maximilian Schmid
Keyword(s):  
Prenatal Testing ◽  
Blood Collection ◽  
Noninvasive Prenatal Testing ◽  
Cell Free Dna ◽  
Free Dna ◽  
Blood Collection Tubes ◽  
Fetal Fraction

scholarly journals Genome-wide study of the effect of blood collection tubes on the cell-free DNA methylome

Epigenetics ◽  
2020 ◽  
pp. 1-11
Author(s):  
Ruben Van Paemel ◽  
Andries De Koker ◽  
Christa Caggiano ◽  
Annelien Morlion ◽  
Pieter Mestdagh ◽  
...  
Keyword(s):  
Blood Collection ◽  
Cell Free Dna ◽  
Dna Methylome ◽  
Free Dna ◽  
Genome Wide ◽  
Blood Collection Tubes ◽  
Genome Wide Study

scholarly journals Optimization of Preanalytical Variables for cfDNA Processing and Detection of ctDNA in Archival Plasma Samples

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Marijana Nesic ◽  
Julie S. Bødker ◽  
Simone K. Terp ◽  
Karen Dybkær
Keyword(s):  
Nucleic Acid ◽  
B Cell Lymphoma ◽  
Circulating Tumor Dna ◽  
Blood Collection ◽  
Plasma Samples ◽  
Cell Free Dna ◽  
Blood Samples ◽  
Free Dna ◽  
The Impact ◽  
Blood Collection Tubes

DNA released from cells into the peripheral blood is known as cell-free DNA (cfDNA), representing a promising noninvasive source of biomarkers that could be utilized to manage Diffuse Large B-Cell Lymphoma (DLBCL), among other diseases. The procedure for purification and handling of cfDNA is not yet standardized, and various preanalytical variables may affect the yield and analysis of cfDNA, including the purification kits, blood collection tubes, and centrifugation regime. Therefore, we aimed to investigate the impact of these preanalytical variables on the yield of cfDNA by comparing three different purification kits DNeasy Blood & Tissue Kit (Qiagen), QIAamp Circulating Nucleic Acid Kit (Qiagen), and Quick-cfDNA Serum & Plasma Kit (Zymo Research). Two blood collection tubes (BCTs), EDTA-K2 and Cell-Free DNA (Streck), stored at four different time points before plasma was separated and cfDNA purified, were compared, and for EDTA tubes, two centrifugation regimes at 2000 × g and 3000 × g were tested. Additionally, we have tested the utility of long-term archival blood samples from DLBCL patients to detect circulating tumor DNA (ctDNA). We observed a higher cfDNA yield using the QIAamp Circulating Nucleic Acid Kit (Qiagen) purification kit, as well as a higher cfDNA yield when blood samples were collected in EDTA BCTs, with a centrifuge regime at 2000 × g . Moreover, ctDNA detection was feasible from archival plasma samples with a median storage time of nine years.

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