A Comparative Histological Study of Wound Healing following Nd:YAG Laser with Different Energy Parameters and Conventional Surgical Incision in Rat Skin

1995 ◽  
Vol 13 (1) ◽  
pp. 11-16 ◽  
Author(s):  
GEORGE E. ROMANOS ◽  
STAVROS PELEKANOS ◽  
JÖRG-RUDOLF STRUB
Keyword(s):  
Wound Healing ◽  
Nd:Yag Laser ◽  
Histological Study ◽  
Rat Skin ◽  
Surgical Incision ◽  
Energy Parameters

Effects of Nd:YAG laser on wound healing processes: Clinical and immunohistochemical findings in rat skin

1995 ◽  
Vol 16 (4) ◽  
pp. 368-379 ◽  
Author(s):  
George E. Romanos ◽  
Stavros Pelekanos ◽  
Jörg-Rudolf Strub
Keyword(s):  
Wound Healing ◽  
Nd:Yag Laser ◽  
Rat Skin ◽  
Healing Processes

In Vivo and ex Vivo Approaches to Studying the Biomechanical Properties of Healing Wounds in Rat Skin

2013 ◽  
Vol 135 (10) ◽  
Author(s):  
Clare Y. L. Chao ◽  
Gabriel Y. F. Ng ◽  
Kwok-Kuen Cheung ◽  
Yong-Ping Zheng ◽  
Li-Ke Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Ex Vivo ◽  
Normal Skin ◽  
Biomechanical Properties ◽  
Skin Wound ◽  
Sprague Dawley ◽  
Rat Skin ◽  
Mechanical Stiffness ◽  
Air Jet

An evaluation of wound mechanics is crucial in reflecting the wound healing status. The present study examined the biomechanical properties of healing rat skin wounds in vivo and ex vivo. Thirty male Sprague-Dawley rats, each with a 6 mm full-thickness circular punch biopsied wound at both posterior hind limbs were used. The mechanical stiffness at both the central and margins of the wound was measured repeatedly in five rats over the same wound sites to monitor the longitudinal changes over time of before wounding, and on days 0, 3, 7, 10, 14, and 21 after wounding in vivo by using an optical coherence tomography-based air-jet indentation system. Five rats were euthanized at each time point, and the biomechanical properties of the wound tissues were assessed ex vivo using a tensiometer. At the central wound bed region, the stiffness measured by the air-jet system increased significantly from day 0 (17.2%), peaked at day 7 (208.3%), and then decreased progressively until day 21 (40.2%) as compared with baseline prewounding status. The biomechanical parameters of the skin wound samples measured by the tensiometer showed a marked reduction upon wounding, then increased with time (all p < 0.05). On day 21, the ultimate tensile strength of the skin wound tissue approached 50% of the normal skin; while the stiffness of tissue recovered at a faster rate, reaching 97% of its prewounded state. Our results suggested that it took less time for healing wound tissues to recover their stiffness than their maximal strength in rat skin. The stiffness of wound tissues measured by air-jet could be an indicator for monitoring wound healing and contraction.

STUDY OF WOUND HEALING IN RATS TREATED WITH SKIN OF POISONOUS FROG, ODORRANA HOSII

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nur Amirah Md Sungif ◽  
Ramlah Zainudin ◽  
Dayangku Norlida Awang Ojep ◽  
Ahmad Hata Rasit
Keyword(s):  
Wound Healing ◽  
Hair Follicle ◽  
Normal Saline ◽  
Antimicrobial Activities ◽  
Skin Secretion ◽  
Rat Skin ◽  
Wound Area ◽  
Biological Dressing ◽  
Defensive Mechanism ◽  
Potential Use

A grafting techniques or using various synthetic and biological dressing also widely used to protect the wound area. There are 8 peptides with differential antimicrobial activities contained in Odorrana hosii’s skin secretion. However, to our best knowledge no study has been scientifically conducted to reveal the value off this species on wound healing. Primarily, the aim of this study was to look at the potential use of O. hosii’s skin as a biological dressing in wound healing management. This study assessed the wound healing in rat compared between wound grafted with O. hosii’s skin and wound treated with normal saline dressing. Histological examination was done to assess the wound healing activities after 14 days. The result shown, both wounds which were treated with O. hosii’s skin and untreated wound heal completely on day 14 as the epidermis and dermis completely close. Histologically, the percentage of neutrophils, macrophages and fibroblasts, were reduced on day 14. However, wounded skin, which was treated with O. hosii’s skin, had better healing quality as more new tissues and hair follicle regrowth compared with the untreated wound. It is suggested that poison gland in the O. hosii’s skin did not harm the wounded rat skin, instead, poison that act as defensive mechanism can help the species to fight the pathogen on the wound.

Onset and Localization of Collagen Synthesis during Wound Healing in Open Rat Skin Wounds

1979 ◽  
Vol 160 (4) ◽  
pp. 458-462 ◽  
Author(s):  
I. K. Cohen ◽  
C. D. Moore ◽  
R. F. Diegelmann
Keyword(s):  
Wound Healing ◽  
Collagen Synthesis ◽  
Skin Wounds ◽  
Rat Skin

scholarly journals Immunohistological changes in skin wounds during the early periods of healing in a rat model

2012 ◽  
Vol 57 (No. 2) ◽  
pp. 77-82 ◽  
Author(s):  
F. Sabol ◽  
L. Dancakova ◽  
P. Gal ◽  
T. Vasilenko ◽  
M. Novotny ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wide Spectrum ◽  
Smooth Muscle Actin ◽  
Healing Process ◽  
Skin Wound ◽  
Hair Follicles ◽  
In Vivo Model ◽  
Wound Healing Process ◽  
Rat Skin

The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin&nbsp;14, &alpha;-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.

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