Antitumor Therapeutic Effects of E7 Subunit and DNA Vaccines in an Animal Cervical Cancer Model: Antitumor Efficacy of E7 Therapeutic Vaccines Is Dependent on Tumor Sizes, Vaccine Doses, and Vaccine Delivery Routes

2006 ◽  
Vol 25 (5) ◽  
pp. 277-286 ◽  
Author(s):  
Jeong-Im Sin ◽  
Sa-Hyun Hong ◽  
Young-Ja Park ◽  
Jae-Bok Park ◽  
Youn Seok Choi ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Vaccines ◽  
Vaccine Delivery ◽  
Antitumor Efficacy ◽  
Therapeutic Effects ◽  
Cancer Model ◽  
Therapeutic Vaccines ◽  
Delivery Routes

scholarly journals Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Samira Sanami ◽  
Fatemeh Azadegan-Dehkordi ◽  
Mahmoud Rafieian-Kopaei ◽  
Majid Salehi ◽  
Maryam Ghasemi-Dehnoo ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
T Lymphocytes ◽  
Tertiary Structure ◽  
Therapeutic Vaccine ◽  
Epitope Prediction ◽  
Therapeutic Effects ◽  
Epitope Vaccine ◽  
In Vivo Experiments

AbstractCervical cancer, caused by human papillomavirus (HPV), is the fourth most common type of cancer among women worldwide. While HPV prophylactic vaccines are available, they have no therapeutic effects and do not clear up existing infections. This study aims to design a therapeutic vaccine against cervical cancer using reverse vaccinology. In this study, the E6 and E7 oncoproteins from HPV16 were chosen as the target antigens for epitope prediction. Cytotoxic T lymphocytes (CTL) and helper T lymphocytes (HTL) epitopes were predicted, and the best epitopes were selected based on antigenicity, allergenicity, and toxicity. The final vaccine construct was composed of the selected epitopes, along with the appropriate adjuvant and linkers. The multi-epitope vaccine was evaluated in terms of physicochemical properties, antigenicity, and allergenicity. The tertiary structure of the vaccine construct was predicted. Furthermore, several analyses were also carried out, including molecular docking, molecular dynamics (MD) simulation, and in silico cloning of the vaccine construct. The results showed that the final proposed vaccine could be considered an effective therapeutic vaccine for HPV; however, in vitro and in vivo experiments are required to validate the efficacy of this vaccine candidate.

scholarly journals DNA vaccines for cervical cancer: from bench to bedside

2007 ◽  
Vol 39 (6) ◽  
pp. 679-689 ◽  
Author(s):  
Chien-Fu Hung ◽  
Archana Monie ◽  
Ronald D Alvarez ◽  
T-C Wu
Keyword(s):  
Cervical Cancer ◽  
Dna Vaccines

The effects of photodynamic therapy with Photodithazine on HPV 16 E6/E7 associated cervical cancer model

2011 ◽  
Vol 15 (03) ◽  
pp. 174-180 ◽  
Author(s):  
Lan Ying Wen ◽  
Su-Mi Bae ◽  
Jin Hwan Do ◽  
Kye-Shin Park ◽  
Woong Shick Ahn
Keyword(s):  
Cervical Cancer ◽  
Photodynamic Therapy ◽  
Growth Inhibition ◽  
Biological Significance ◽  
Light Irradiation ◽  
Tumor Growth Inhibition ◽  
Cancer Model ◽  
Hpv 16

Photodynamic therapy (PDT) is a promising treatment for cancer that has been recently accepted in the clinic. In this study, we examined a biological significance of PDT with a chlorin-based photosensitizer, Photodithazine, on cervical cancer model. When human papillomavirus type 16 (HPV16)- transformed mouse TC-1 cells were exposed to varied doses of Photodithazine with light irradiation (6.25 J/cm2), the significant growth inhibition of TC-1 cells was observed at 0.75 μg/mL of Photodithazine. The damaged cells by Photodithazine/PDT were categorized to be early and late apoptosis, as determined by annexin V staining. Photodithazine was primarily localized at lysosome apparatus within TC-1 cells while it was rapidly accumulated and sustained for initial 3 h in tumor tissue of TC-1 tumor bearing mice after IV injection. The tumor growth inhibition by Photodithazine/PDT with light irradiation (300 J/cm2) was examined after injection of various concentration of Photodithazine in tumor mice system. Our results show that Photodithazine/PDT might have significant advantages in the selective killing of tumor lesions in HPV 16 E6/E7 associated cervical cancer model, both in vitro and in vivo.

Advances in dendritic cell-based therapeutic vaccines for cervical cancer

2007 ◽  
Vol 7 (10) ◽  
pp. 1473-1486 ◽  
Author(s):  
Stefania Bellone ◽  
Sergio Pecorelli ◽  
Martin J Cannon ◽  
Alessandro D Santin
Keyword(s):  
Cervical Cancer ◽  
Dendritic Cell ◽  
Therapeutic Vaccines

Antitumor effects of internal iliac arterial infusion of platinum compounds in a rabbit cervical cancer model

1997 ◽  
Vol 89 (2) ◽  
pp. 286-290 ◽  
Author(s):  
H ITAMOCHI ◽  
J KIGAWA ◽  
Y MINAGAWA ◽  
X CHENG ◽  
M OKADA ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Platinum Compounds ◽  
Cancer Model ◽  
Antitumor Effects ◽  
Arterial Infusion ◽  
Internal Iliac

scholarly journals Therapeutic effects of adoptive splenocyte transfer following in situ AdIL-12 gene therapy in a mouse prostate cancer model

2005 ◽  
Vol 13 (1) ◽  
pp. 91-98 ◽  
Author(s):  
T Saika ◽  
N Kusaka ◽  
V Mouraviev ◽  
T Satoh ◽  
H Kumon ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gene Therapy ◽  
Therapeutic Effects ◽  
Cancer Model ◽  
Mouse Prostate ◽  
Prostate Cancer Model

scholarly journals Three-Dimensional Printing of a Hemorrhagic Cervical Cancer Model for Postgraduate Gynecological Training

Cureus ◽  
2017 ◽  
Author(s):  
Michael Bartellas ◽  
Stephen Ryan ◽  
Gregory Doucet ◽  
Deanna Murphy ◽  
Jacqueline Turner
Keyword(s):  
Cervical Cancer ◽  
Three Dimensional ◽  
Cancer Model ◽  
Three Dimensional Printing ◽  
Dimensional Printing

Chemopreventive and Antitumor Efficacy of Curcumin in a Spontaneously Developing Hen Ovarian Cancer Model

2017 ◽  
Vol 11 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Kazim Sahin ◽  
Cemal Orhan ◽  
Mehmet Tuzcu ◽  
Nurhan Sahin ◽  
Hakkı Tastan ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Antitumor Efficacy ◽  
Cancer Model

Photodynamic effects of Radachlorin® on cervical cancer model

2005 ◽  
Vol 09 (12) ◽  
pp. 835-840 ◽  
Author(s):  
Sun-Young Kwak ◽  
Dae-Seog Lim ◽  
Su-Mi Bae ◽  
Yong-Wook Kim ◽  
Joon-Mo Lee ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Golgi Apparatus ◽  
Biological Significance ◽  
Annexin V ◽  
Light Irradiation ◽  
Control Group ◽  
Cancer Model ◽  
In Vivo Experiments

Photodynamic therapy (PDT) has been reported to be effective for treating various tumors and induce apoptosis in many tumor cells. In this study, we examined a biological significance of PDT with a chlorin-based photosensitizer, Radachlorin®, in a cervical cancer model, TC-1 cells. When TC-1 cells were exposed to varied doses of Radachlorin® with light irradiation (6.25 J/cm2), PDT induced a dose-dependent growth inhibition of TC-1 cells. All of these cells were significantly damaged after light irradiation and categorized to be early and late apoptosis, as determined by annexin V staining. Radachlorin® localized primarily into the Golgi apparatus of cells in 12 h of the treatment, and weak fluorescence intensity was also detected in mitochondria. On the other hand, in the in vivo experiments, following light irradiation (100 J/cm2), retarded tumor growth was significant in mice treated with Radachlorin®, as compared to the control group. Taken together, we propose that PDT after the application of Radachlorin® may induce the Golgi apparatus-mediated apoptosis of cervical cancer cells in vitro, and also be effective in the mice system.

Sign in / Sign up

Export Citation Format

Share Document