Microbial Sand Stabilization Using Corn Steep Liquor Culture Media and Industrial Calcium Reagents in Cementation Solutions

Effects of corn steep liquor (organic fertilizer, OF) and conventional chemical fertilizer (CF) on the growth and yield of bok choy (Brassica rapa var. chinensis) in summer and autumn hydroponic growing systems were compared. When OF and CF were applied with the same amount of total nitrogen in summer cultivation, there was no significant difference between yields; however, the growth rate in OF was slower than in CF. When OF was applied with twice the amount of nitrogen in CF (OF2), bok choy growth and yield were significantly inhibited in summer cultivation, likely owing to dissolved oxygen deficiency and different rates of nitrification and nitrogen absorbance by the plant root. Although the contents of potassium, calcium, and magnesium in bok choy showed no difference among the three treatments in both cultivation seasons, the carbon/nitrogen ratio tended to be higher in OF and OF2 than in CF. Lower nitric acid and higher ascorbic acid content was found in OF and OF2 than in CF. Overall, our results suggest that a comparable yield is expected by using the same nitrogen amount with a conventional recipe of chemical fertilization in autumn cultivation. However, further improvement of hydroponic management is needed in summer cultivation.

Download Full-text

Effects of corn steep liquor on β-poly(l-malic acid) production in Aureobasidium melanogenum

AMB Express ◽

10.1186/s13568-020-01147-8 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Genan Wang ◽

Bingyi Shi ◽

Pan Zhang ◽

Tingbin Zhao ◽

Haisong Yin ◽

...

Keyword(s):

Malic Acid ◽

Low Cost ◽

Corn Steep Liquor ◽

Enrichment Analysis ◽

Tca Cycle ◽

Water Soluble ◽

Growth And Yield ◽

The Tca Cycle ◽

Steep Liquor ◽

Aureobasidium Melanogenum

Abstractβ-poly(l-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Yp/x) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.

Download Full-text

Production of 1,3-propanediol by Clostridium beijerinckii DSM 791 from crude glycerol and corn steep liquor: Process optimization and metabolic engineering

Bioresource Technology ◽

10.1016/j.biortech.2016.04.020 ◽

2016 ◽

Vol 212 ◽

pp. 100-110 ◽

Cited By ~ 42

Author(s):

Daiana Wischral ◽

Jianzhi Zhang ◽

Chi Cheng ◽

Meng Lin ◽

Lucas Monteiro Galotti De Souza ◽

...

Keyword(s):

Metabolic Engineering ◽

Process Optimization ◽

Crude Glycerol ◽

Corn Steep Liquor ◽

Clostridium Beijerinckii ◽

Steep Liquor

Download Full-text

Synthesis of the Microbial Polysaccharide Gellan from Dairy and Plant-Based Processing Coproducts

Polysaccharides ◽

10.3390/polysaccharides2020016 ◽

2021 ◽

Vol 2 (2) ◽

pp. 234-244

Author(s):

Thomas P. West

Keyword(s):

Corn Steep Liquor ◽

Nitrogen Sources ◽

Soluble Starch ◽

Culture Conditions ◽

Water Soluble ◽

Production Costs ◽

Microbial Polysaccharide ◽

Steep Liquor ◽

The Cost ◽

Condensed Distillers Solubles

This review examines the production of the microbial polysaccharide gellan, synthesized by Sphingomonas elodea, on dairy and plant-based processing coproducts. Gellan is a water-soluble gum that structurally exists as a tetrasaccharide comprised of 20% glucuronic acid, 60% glucose and 20% rhamnose, for which various food, non-food and biomedical applications have been reported. A number of carbon and nitrogen sources have been tested to determine whether they can support bacterial gellan production, with several studies attempting to optimize gellan production by varying the culture conditions. The genetics of the biosynthesis of gellan has been explored in a number of investigations and specific genes have been identified that encode the enzymes responsible for the synthesis of this polysaccharide. Genetic mutants exhibiting overproduction of gellan have also been identified and characterized. Several dairy and plant-based processing coproducts have been screened to learn whether they can support the production of gellan in an attempt to lower the cost of synthesizing the microbial polysaccharide. Of the processing coproducts explored, soluble starch as a carbon source supported the highest gellan production by S. elodea grown at 30 °C. The corn processing coproducts corn steep liquor or condensed distillers solubles appear to be effective nitrogen sources for gellan production. It was concluded that further research on producing gellan using a combination of processing coproducts could be an effective solution in lowering its overall production costs.

Download Full-text