Boosting Angiogenesis and Functional Vascularization in Injectable Dextran–Hyaluronic Acid Hydrogels by Endothelial-Like Mesenchymal Stromal Cells

Mesenchymal stromal cells (MSCs) are currently used for cartilage cell therapy because of their well proven capacity to differentiate in chondrocytes. The advantage of MSC-based therapy is the possibility of producing a high number of chondrocytes for implants. The transplant procedure, however, has some limitations, since MSCs may produce non-functional chondrocytes. This limit has been challenged by cultivating MSC in media with hydrogels containing hyaluronic acid (HA), extractive chondroitin sulfate (CS), or bio-fermentative unsulphated chondroitin (BC) alone or in combination. Nevertheless, a clear study of the effect of glycosaminoglycans (GAGs) on chondrocyte differentiation is still lacking, especially for the newly obtained unsulfated chondroitin of biotechnological origin. Are these GAGs playing a role in the commitment of stem cells to chondrocyte progenitors and in the differentiation of progenitors to mature chondrocytes? Alternatively, do they have a role only in one of these biological processes? We evaluated the role of HA, CS, and – above all – BC in cell commitment and chondrocyte differentiation of MSCs by supplementing these GAGs in different phases of in vitro cultivation. Our data provided evidence that a combination of HA and CS or of HA and BC supplemented during the terminal in vitro differentiation and not during cell commitment of MSCs improved chondrocytes differentiation without the presence of fibrosis (reduced expression of Type I collagen). This result suggests that a careful evaluation of extracellular cues for chondrocyte differentiation is fundamental to obtaining a proper maturation process.

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Mineralization behavior with mesenchymal stromal cells in a biomimetic hyaluronic acid-based scaffold

Biomaterials ◽

10.1016/j.biomaterials.2010.01.148 ◽

2010 ◽

Vol 31 (14) ◽

pp. 3986-3996 ◽

Cited By ~ 34

Author(s):

Cristina Manferdini ◽

Vincenzo Guarino ◽

Nicoletta Zini ◽

Maria Grazia Raucci ◽

Andrea Ferrari ◽

...

Keyword(s):

Hyaluronic Acid ◽

Mesenchymal Stromal Cells ◽

Stromal Cells

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Biopolymers-calcium phosphate antibacterial coating reduces the pathogenicity of internalized bacteria by mesenchymal stromal cells

Biomaterials Science ◽

10.1039/d0bm00962h ◽

2020 ◽

Vol 8 (20) ◽

pp. 5763-5773

Author(s):

Marie Dubus ◽

Jennifer Varin-Simon ◽

Pierre Prada ◽

Loïc Scomazzon ◽

Fany Reffuveille ◽

...

Keyword(s):

Hyaluronic Acid ◽

Cell Adhesion ◽

Calcium Phosphate ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Stromal Cell ◽

Antibacterial Coating

Coating made of calcium phosphate/chitosan/hyaluronic acid, proposed as an antibacterial substrate for stromal cell adhesion.

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Oral Mesenchymal Stromal Cells in Systemic Sclerosis: Characterization and Response to a Hyaluronic-Acid-Based Biomaterial

Applied Sciences ◽

10.3390/app11178101 ◽

2021 ◽

Vol 11 (17) ◽

pp. 8101

Author(s):

Alina Stanomir ◽

Carmen Mihaela Mihu ◽

Simona Rednic ◽

Cristina Pamfil ◽

Alexandra Roman ◽

...

Keyword(s):

Hyaluronic Acid ◽

Systemic Sclerosis ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Culture Media ◽

Isolated Cells ◽

Normal Medium ◽

Mesenchymal Differentiation ◽

Migration Potential

Introduction. As oral mesenchymal stromal cells (MSCs) have not, to date, been isolated from systemic sclerosis (SSc) patients, the aim of this in vitro experiment was to characterize gingival MSCs (SScgMSCs) and granulation tissue MSCs (SScgtMSCs) from SSc and to evaluate their functionality in comparison to healthy MSCs (hMSCs), in normal or hyaluronic acid (HA) culture media. Materials and Methods. Isolated cells were described by immunophenotyping of surface antigen make-up and by trilineage mesenchymal differentiation capacity. Colony-Forming Unit-Fibroblast (CFU-F) test and migration potential evaluated MSC functionality. Results. All types of MSCs displayed positivity for the following surface markers: CD29, CD73, CD90, CD105, CD44, and CD79a. These cells did not express CD34, CD45, HL-DR, and CD14. Isolated MSCs differentiated into osteoblasts, adipocytes, and chondroblasts. The frequency of CFU-F for SScgtMSCs was significantly lower than that of hMSCs (p = 0.05) and SScgMSCs (p = 0.004) in normal medium, and also markedly lower than that of SScgMSCs (p = 0.09) in HA medium. Following HA exposure, both SScgMSCs and SScgtMSCs migrated significantly less (p = 0.033 and p = 0.005, respectively) than hMSCs. Conclusions. A reduced functionality of MSCs derived from SSc as compared to hMSCs was observed. HA in culture medium appeared to significantly stimulate the migration potential of hMSCs.

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