scholarly journals Laboratory Evaluation of Two IGR Insecticides Against DBM Larvae, 1995

1997 ◽  
Vol 22 (1) ◽  
pp. 411-411
Author(s):  
R.F.L. Mau ◽  
L. R. Gusukuma-Minuto
Keyword(s):  
Petri Dish ◽  
Seedling Stage ◽  
Test Material ◽  
Laboratory Evaluation ◽  
Complete Coverage ◽  
Plastic Petri Dish ◽  
True Leaf ◽  
Laboratory Colony ◽  
Previously Treated ◽  
Head Cabbage

Abstract Treatments were evaluated using the leaf dip method. Head cabbage was seeded in community pots. Each pot containing approximately 10 cabbage plants in the 5-7 true leaf seedling stage was inverted and dipped in a solution of the test materials for about 30 sees for complete coverage. The dipped plants were allowed to air dry. For each dip, 1 liter solution of the test material was prepared based on field rate concentrations of 100 gal/acre. Leaves from treated plants were detached and placed in a ventilated plastic petri dish. DBM larvae from a laboratory colony that originated from individuals collected from a cabbage field at Kula, Hawaii and Kamuela, Hawaii were used. Ten 2nd instars were placed on each leaf. Each treatment was replicated 10 times. Xentari was used as the treated check. The number of dead larvae was counted at 24-hour intervals. Larvae were recorded as dead when there was no movement when probed. Leaves were changed every other day with fresh leaves from the previously treated pots.

scholarly journals Laboratory Evaluation of a Neem Product Against DBM Larvae, 1995

1997 ◽  
Vol 22 (1) ◽  
pp. 409-409
Author(s):  
R.F.L. Mau ◽  
L. R. Gusukuma-Minuto
Keyword(s):  
Petri Dish ◽  
Seedling Stage ◽  
Laboratory Evaluation ◽  
Complete Coverage ◽  
Plastic Petri Dish ◽  
True Leaf ◽  
Laboratory Colony ◽  
Treated Plant ◽  
Head Cabbage

Abstract Treatments were evaluated using the leaf dip method. Head cabbage was seeded in community pots. Each pot containing approximately 10 cabbage plants in the 5 true leaf seedling stage was inverted and dipped in a test insecticide mix for about 30 sees for complete coverage. The dipped plants were allowed to air dry. For each dip, 1 liter insecticide mix was prepared based on field rate concentrations of 100 gal/acre. Leaves from treated plants were detached and placed in a ventilated plastic petri dish. DBM larvae from a laboratory colony that originated from individuals collected from a cabbage field at Kula, Hawaii and Kamuela, Hawaii were used. Ten late 2nd instars were placed on each leaf. Fresh leaves from the original treated plant were added every two days. The number of dead larvae was counted at 24-hour intervals. Larvae were recorded as dead when there was no movement when probed.

scholarly journals Laboratory Evaluations of Bioinsecticides Against Dbm Larvae, 1994

1995 ◽  
Vol 20 (1) ◽  
pp. 327-327
Author(s):  
R. F. L. Mau ◽  
L. R. Gusukuma-Minuto ◽  
R. S. Shimabuku
Keyword(s):  
Petri Dish ◽  
Seedling Stage ◽  
Complete Coverage ◽  
Plastic Petri Dish ◽  
True Leaf ◽  
Laboratory Colony ◽  
Treated Plant ◽  
Newly Hatched Larvae ◽  
Head Cabbage ◽  
Respective Solution

Abstract Treatments were evaluated using the leaf dip method. Head cabbage was seeded in community pots. Each pot contained approximately 10 cabbage plants. Potted head cabbage in the 5 true leaf seedling stage were dipped in each respective solution for complete coverage and allowed to air dry. Leaves from treated plants were detached and placed in a ventilated plastic petri dish. DBM larvae from a laboratory colony that originated from individuals collected from a cabbage field at Kula, Hawaii were used. Ten newly hatched larvae were placed on each leaf. Fresh leaves from the original treated plant were added every two days. The number of dead larvae was counted at 24 or 48 hour intervals. Larvae were recorded as dead when there was no movement when probed.

TOXICITY OF INSECTICIDES TO THE ALFALFA LEAFCUTTER BEE, MEGACHILE ROTUNDATA F. (HYMENOPTERA: MEGACHILIDAE), ESTABLISHED BY THREE EVALUATION METHODS

1988 ◽  
Vol 120 (4) ◽  
pp. 297-305 ◽  
Author(s):  
W. A. Charnetski
Keyword(s):  
Petri Dish ◽  
Evaluation Methods ◽  
Laboratory Evaluation ◽  
Evaluation Procedure ◽  
Megachile Rotundata ◽  
Field Cage ◽  
Male And Female ◽  
Significant Difference ◽  
Leafcutter Bee ◽  
Significant Mortality

AbstractThree methods—tube chambers (ventilated and unventilated), petri dish chambers, and field cages—were used to evaluate the toxicity of deltamethrin, trichlorfon, and methoxychlor deposits on alfalfa, Medicago sativa L., to 1- to 7-day-old alfalfa leafcutter bees, Megachile rotundata (F.), 24 and 48 h after exposure. In unventilated tube chambers, all three insecticides were significantly toxic to male bees after 24 and 48 h, but only deltamethrin and trichlorfon were significantly toxic to female bees after 24 h. In ventilated tube chambers, only deltamethrin was significantly toxic by contact and then only to male bees at 24 h. Significant vapor action was observed only for trichlorfon and only in unventilated tube chambers. By the petri dish method, only deltamethrin caused significant mortality to male and female bees. However, bee mortality increased significantly between 24 and 48 h. By the field cage method, there was no significant difference in mortality among treatments and controls within the 24- and 48-h evaluations. Bee mortality in the controls was much higher in the petri dish and field cage methods than in either of the two variations of the tube chamber method. The three evaluation methods are compared and the need for a standardized laboratory evaluation procedure is discussed.

scholarly journals Ovicidal action of insectoacaricide drugs sentry home, Neostomazan 1:200 manufacured by CEVA, Neostomazan 1:200 manufacured by product and Extrazol M on fleas Ctenocephalides spp. eggs

2021 ◽  
pp. 111-118
Author(s):  
Olga Yasynovska
Keyword(s):  
Veterinary Medicine ◽  
Light Microscope ◽  
Petri Dish ◽  
Test Material ◽  
Innovative Technologies ◽  
Ovicidal Effect ◽  
Livestock Products

The aim of the research. To compare the ovicidal efficiency of insectoacaricides of different composition and manufacturers on flea eggs (Ctenocephalides spp.) for treatemnt of the premises where animals live. Materials and methods of the research. The study was conducted on the basis of the clinic of veterinary medicine "Vetservice" Sumy, laboratory "Veterinary Pharmacy" and "Innovative technologies and safety and quality of livestock products" of Sumy National Agrarian University. The ovicidal effect of insectoacaricides on flea eggs of Ctenocephalides spp. was studied. Ctenocephalides spp. eggs were selected from the pet bedding on which the animal spended most of its time, namely the cats. The studied material was selected with a cosmetic brush. Ctenocephalides spp. eggs were placed into a Petri dish of 10 eggs per each dish. The test material was introduced with a dental probe. There were 4 test dishes, which were treated with insectoacaricides (each test dish was treated with a separate drug) and 1 control dish with no treatment. Microscopy was conducted under a light microscope with magnification X8 of each egg, with following treatment of each egg with insectoacaricides. Monitoring was conducted in 24, 48 and 72 hours after treatment. Results. Research has shown that drugs which demonstrated 100 % ovicidal effectiveness were Sentry Home (pyriproxyfen – 0.02 %, permethrin – 0.2 %, n-Octyl Bicyclohepten – 1.0 %) in 24 hours and Neostomazan (CEVA)(transmix – 5.0 g, tetramethrin – 0.5 g) in 72 hours. Conclusions. Insectoacaricide drug Sentry Home (pyriproxyfen – 0.02 %, permethrin – 0.2 %, n-Octyl Bicyclohepten – 1.0 %), used for the treatment of the premises where the animals live, showed the most pronounced ovicidal effect in 24 hours.

THE INFLUENCE OF VARIOUS TEMPERATURES AND (2-CHLOROETHYL) TRIMETHYLAMMONIUM CHLORIDE AND (ALLYL) TRIMETHYLAMMONIUM BROMIDE ON PEPPERS AND TOMATOES

1962 ◽  
Vol 42 (1) ◽  
pp. 142-149 ◽  
Author(s):  
H. Tiessen
Keyword(s):  
Total Yield ◽  
Temperature Treatment ◽  
Seedling Stage ◽  
Trimethylammonium Chloride ◽  
Green Plants ◽  
True Leaf ◽  
Different Temperatures ◽  
Lower Temperature ◽  
Field Setting ◽  
Pepper Plants

Fireball tomato and Calwonder pepper plants were subjected, in the seedling stage, to different temperatures and to both drench and foliar applications of (2-chloroethyl) trimethylammonium chloride (CCC) and (allyl) trimethylammonium bromide (AMAB).Seedlings were exposed to minimum night temperatures of 54° to 56°F. and of 64° to 68°F. from the appearance of the first true leaf for 3 weeks for peppers and until field setting (6 weeks) for tomatoes. For both crops the lower temperature treatment gave an increase in early fruit number and yield.Drench applications of 100 milliliters per band to tomatoes and 900 milliliters per square foot to peppers of 10−3 and 10−4 M solutions of CCC or of AMAB produced shorter, stockier, darker-green plants with reduced foliage spread. Growth was further reduced by the lower temperature treatments. Both drenches reduced total yields in both tomatoes and peppers.Foliar treatments of CCC and of AMAB increased the total yield of peppers started at the warmer temperature. At the cooler temperature, however, yield was not affected by the CCC treatment but was reduced by the AMAB treatment. Foliar treatments with either chemical did not affect the total yield of tomatoes after seedling treatment at either temperature.

scholarly journals Differences in Effectiveness of Antibacterial Power Between Cocoa Peel Extract (Theobroma cocoa L.) and Benzalkonium Chloride 0.1% Against Staphylococcus aureus (In Vitro)

2021 ◽  
Vol 11 (2) ◽  
pp. 56
Author(s):  
Tamara Yuanita ◽  
Mohammed Alaqsha Brysoul Ceson ◽  
Agus Subiyanto
Keyword(s):  
Staphylococcus Aureus ◽  
Benzalkonium Chloride ◽  
Petri Dish ◽  
Test Material ◽  
Control Group ◽  
Control Group Design ◽  
Dental Biofilm ◽  
Significant Difference ◽  
Peel Extract

Background: Staphylococcus aureus is a gram-positive bacteria play a role in the formation of dental biofilm which iscausing dental caries. During tooth preparation, to stop the growth of bacteria, a cavity cleaning agent is given using achemical, namely Benzalkonium Chloride (BAC) 0.1%, but BAC has disadvantages including allergic reactions, tolerantmicrobes, and resistance. Therefore, it is hoped that there will be herbal ingredients that can be used as an alternative.Cocoa peel extract has active compounds of tannins, flavonoids, alkaloids, terpenoids, and saponins which haveantibacterial concentration 6% according to safe concentrations. Purpose: To explain the difference in the effectivenessof the antibacterial power of 6% cocoa peel extract and 0.1% BAC against Staphylococcus aureus (in vitro). Methods:This study was a laboratory experimental in vitro with the posttest only control group design. Using the diffusion methodfor Staphylococcus aureus that divided into two parts, 6% cocoa peel extract and 0.1% BAC. Each petri dish was givendisc paper dripped with 0.01 ml of each test material, then incubated for two days and observed the diameter of theinhibition zone. Results: The average diameter of the inhibition zone formed in the 6% cacao peel extract was 11.5288mm and BAC 0.1% was 18.2925 mm against Staphylococcus aureus. Conclusion: There was a significant difference inthe effectiveness of antibacterial power (p <0.05) between 6% cacao peel extract (Theobroma cacao L.) and 0.1% BACagainst Staphylococcus aureus (In Vitro).

scholarly journals The sperm stewing in its own ROS—in the plastic Petri dish

2017 ◽  
Vol 5 (17) ◽  
pp. 366-366 ◽  
Author(s):  
Horst-Dieter Försterling ◽  
Attila E. Pavláth ◽  
Ádám R. Mester ◽  
Antonio L. B. Pinheiro ◽  
Mario A. Trelles
Keyword(s):  
Petri Dish ◽  
Plastic Petri Dish
Sign in / Sign up

Export Citation Format

Share Document