131P A comparative study on cytotoxicity of nanomaterials: decreased cell viability induced by carbon nanotubes and graphen oxide in HepG2 cell line

Abstract Objective In present study, the effects of the leaf extract of Pyrus biossieriana Buhse on tert-Butyl hydroperoxide (t-BHP) induced toxicity in the HepG2 cell line were investigated. Results HepG2 cells were exposed to different concentrations of both extract (1.5, 2.0, and 2.5 mg/mL) and t-BHP (100, 150, and 200 μM). The total flavonoid and phenolic contents, the cell viability, lipid peroxidation, NO generation, and the total antioxidant capacity in cell media were assessed. The amount of arbutin was estimated 12.6% of the dry weight of leaves (equivalent to 126 mg/g). Additionally, the amounts of flavonoids and phenols in extract were estimated 119 mg/g and 418 mg/g, respectively. The cells incubated with t-BHP showed a significant decrease in survival (p < 0.001). Preincubation with extract (1.5 mg/mL and 2.0 mg/mL) attenuated the t-BHP toxicity and increased the cell viability in cells exposed even to the highest concentration of t-BHP (200 μM) (p value < 0.001, and p value = 0.035) respectively. Additionally, treatment with extract reduced the cell growth suppression caused by t-BHP. The P. biossieriana Buhse leaf extract at concentrations of 1.5 and 2.0 mg/mL is capable of attenuating t-BHP-induced cytotoxicity in HepG2 cells.

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Curcumin Protects HepG2 Cells from the Cytotoxic Effect of Drugs with Anti-fibrotic Activity

10.21203/rs.3.rs-289436/v1 ◽

2021 ◽

Author(s):

Mariana Y. Medina-Pizaño ◽

Marina N. Medina-Rosales ◽

Esperanza Sánchez-Alemán ◽

Sandra L. Martínez-Hernández ◽

Liseth R. Aldaba-Muruato ◽

...

Keyword(s):

Cell Viability ◽

Cell Line ◽

Hepg2 Cell ◽

Hepg2 Cells ◽

Morphological Changes ◽

Liver Parenchyma ◽

Hepg2 Cell Line ◽

Secondary Effects ◽

Hamster Model ◽

Hematoxylin And Eosin

Abstract Background: The α and β adrenoblockers have been tested as an alternative treatment for chronic liver lesions such as fibrosis and cirrhosis in animal models, as well as their possible participation during the regeneration of the damage caused by liver cirrhosis in a hamster model. However, it was observed that doxazosin caused slight morphological changes in hepatocytes, while that curcumin showed protection to the hepatic parenchyma. Regardless, the pharmacokinetic effects of these 𝛼/𝛽 adrenoblockers on the hepatocytes' cell viability, possibly involved in the hepatic parenchyma's repopulation during cirrhosis reversal, are unknown. The present study aimed to elucidate the protective effect of curcumin on the possible side effects of doxazosin, tamsulosin, and carvedilol on the HepG2 cell line, drugs already tested with antifibrotic activity.Methods: HepG2 cells were exposed to 0.1, 0.5, 10, and 25 µM of doxazosin, carvedilol, and tamsulosin for 24, 48, and 72 h, for curcumin, cells were pretreated with 1 µM for 1 h before exposure to α and β adrenoblockers. The cell viability was assessed by MTT assay. The morphological changes were determined using hematoxylin and eosin (H&E) staining, scanning electron microscope (SEM), and acridine orange (AO) staining. Results: We observed that the doxazosin decreases cell viability dependently time and dose; carvedilol and tamsulosin increase cell proliferation. However, curcumin induces regulation of these effects in HepG2 cell line, increasing or maintaining viability compared to control. The pretreatment with curcumin regulated AST levels (aspartate aminotransferase) and ALT (alanine aminotransferase) in cells exposed to α and β adrenoblockers. The SEM and H&E staining provided evidence that doxazosin, carvedilol, and tamsulosin induced morphological changes in HepG2 cell line, depending on time and dose, approximately 80% of the cells treated with drugs were balonized, and curcumin protected these effects, maintaining the morphology in 90% of the treated cells.Conclusions: The present study demonstrates that curcumin protected the HepG2 cells against cytotoxicity and morphological changes induced by the α and β adrenoblockers attenuating secondary effects for possible oxidative stress. In this way, it is concluded that these treatments with antifibrotic effect, in co-treatment with curcumin, will not affect the possible repopulation process of the liver parenchyma during the reversion of fibrosis.

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Hepatoprotective activity of Flacourtia jangomas (Lour.) Raeuschleaves and fruit methanolic extract on paracetamol-induced hepatotoxicity in HepG2 Cells

Biomedicine ◽

10.51248/.v41i3.1197 ◽

2021 ◽

Vol 41 (3) ◽

pp. 587-591

Author(s):

Akshaya Pai ◽

Chandrakala Shenoy

Keyword(s):

Cell Viability ◽

Cell Line ◽

Hepg2 Cell ◽

Hepg2 Cells ◽

Methanolic Extract ◽

Hepatoprotective Activity ◽

Negative Control ◽

Hepg2 Cell Line ◽

Fruit Extract ◽

Deciduous Fruit

Introduction and Aim: Plants have become the current focus of research in treating the various diseases and ailments. Flacourtia jangomas (Lour.) Raeusch belongs to the familySalicaceae. Itis a small deciduous fruit tree having immense nutritional and medicinal significance. Different parts of the plant are pharmaceutically used forcuring various ailments. In this study, we investigated the hepatoprotective activity of Flacourtia jangomas (Lour.) Raeusch leaves and fruit methanolic extract on Paracetamol induced HepG2 cell line. Methods: The cytotoxic and hepatoprotective properties were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); lipid peroxidation (malondialdehyde (MDA) levels). Results:The increased cell viability of 140.43± 4.07% and 133.93±3.20%was observed in HepG2 cells treated with methanolic extract of F. jangomas leaf and fruit extract respectively at 10µg/ml concentration and then decreased along with the rise of F. jangomas leaf and fruit extract concentrations. The level of LDH, ALT, AST and MDA decreased after F. jangomas leaf and fruit treatment compared to negative control. Conclusion: This study suggests that the methanolic Extract of F. jangomas (Lour.) Raeusch leaves(FJL)and fruit (FJF) shows hepatoprotective activity in Paracetamol induced HepG2 cell line by the decrease in AST and ALT activities and LDH and MDA level. Hence, it could be considered as a therapeutic agent in curing liver-related diseases.

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