Commentary on: Fat Graft Enhanced With Adipose-Derived Stem Cells in Aesthetic Breast Augmentation: Clinical, Histological, and Instrumental Evaluation

2020 ◽  
Vol 40 (9) ◽  
pp. 978-980
Author(s):  
Alexandra Condé-Green
Keyword(s):  
Stem Cells ◽  
Breast Augmentation ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Instrumental Evaluation ◽  
Aesthetic Breast

Fat Graft Enhanced With Adipose-Derived Stem Cells in Aesthetic Breast Augmentation: Clinical, Histological, and Instrumental Evaluation

2019 ◽  
Vol 40 (9) ◽  
pp. 962-977 ◽  
Author(s):  
Pietro Gentile ◽  
Ashutosh Kothari ◽  
Donato Casella ◽  
Claudio Calabrese
Keyword(s):  
Stem Cells ◽  
Soft Tissue ◽  
Breast Augmentation ◽  
Preoperative Evaluation ◽  
Fat Graft ◽  
Control Group ◽  
Adipose Derived Stem Cells ◽  
Level Of Evidence ◽  
Breast Hypoplasia ◽  
3D Volume

Abstract Background Fat graft enhanced with adipose-derived stem cells (FG-e-ASCs) has been utilized in outcomes of radiotherapy after mastectomy, breast soft tissue defects, ulcers, and loss of substance. The authors present their experience utilizing FG-e-ASCs in breast augmentation. Objectives The aim of this study was to evaluate the safety and efficacy of a study group (SG) regarding utilization of FG-e-ASCs in breast augmentation for aesthetic improvement, comparing the results with a control group (CG). Methods A total of 46 patients affected by breast hypoplasia were treated with FG-e-ASCs, comparing results with those of a CG (n = 30) treated with fat graft not enhanced with adipose-derived stem cells (FG-ne-ASCs). The preoperative evaluation included a complete clinical evaluation, a photographic assessment, magnetic resonance imaging of the soft tissue, ultrasound, and mammography. Postoperative follow-up took place at 1, 3, 7, 12, 24, and 48 weeks and then annually. Results The patients treated with FG-e-ASCs showed 58% maintenance of the contour restoring and of 3-dimensional (3D) volume after 3 years compared with the patients of the CG treated with FG-ne-ASCs, who showed 29% maintenance. In 67.4% (n = 31) of breast augmentations treated with FG-e-ASCs, we observed a restoration of the breast contour and an increase of 10.3 mm in the 3D volume after 36 months, which was observed in only 20.0% (n = 6) of patients in the CG treated with FG-ne-ASCs. Volumetric persistence in the SG was higher than that in the CG (P < 0.0001 SG vs CG). Conclusions Utilization of FG-e-ASCs was safe and effective in this series of cases performed. Level of Evidence: 4

scholarly journals Fat graft – the natural choice for reconstructive, regenerative and aesthetic surgery

2017 ◽  
Vol 5 (2) ◽  
pp. 113-117
Author(s):  
Samir Ibrahim ◽  
Joanna Rybacka-Mossakowska ◽  
Sławomir Michalak
Keyword(s):  
Stem Cells ◽  
Donor Site ◽  
Aesthetic Surgery ◽  
Fat Grafting ◽  
Fat Graft ◽  
Clinical Effects ◽  
Adipose Derived Stem Cells ◽  
Safe Alternative ◽  
Natural Choice ◽  
Reconstructive Operations

AbstractThe search for appropriate filler, which can be used for aesthetic and reconstructive operations is currently one of challenges for plastic surgery. The application of absorbable and permanent artificial fillers may cause adverse events. Thus, autologous fat grafting can be a safe alternative. Moreover, fat tissue is rich in adipose-derived stem cells (ASC), which can be successfully used for regenerative procedures. The paper reviews reports on fat grafting procedures, which indicate risks and their possible prophylactic.Adipose tissue is a much more prolific source of ASCs than bone marrow. Basically, ASC are characterized by a spectrum of markers: CD11b-CD45-CD13+CD73+CD90+, which can be widened by CD36+CD-106-CD10+CD26+CD49d+CD49e+CD3-D49f -PODXL- to improve phenotyping. It is suggested to use at least two negative markers and two positive markers during the same phenotyping analysis. Fat transfer requires appropriate approach, planning and technique to make it clinically successful.Fat grafting fulfills the expectations for ideal injectable agent, which can be used for aesthetic and reconstructive surgery. To improve the survival of fat graft, careful decisions on donor site, local anesthetic administration, liposuction method, processing and placement methods need to be made. Moreover, fat is the source of adipose-derived stem cells which can be used for regenerative procedures. A proper transformation and identification of those cells is required to improve clinical effects.

Synergistic Effect of Adipose-Derived Stem Cells and Fat Graft on Wrinkles in Aged Mice

2019 ◽  
Vol 143 (6) ◽  
pp. 1637-1646 ◽  
Author(s):  
Kikap Kim ◽  
Yingfang Fan ◽  
Guang Lin ◽  
Yoon Kyung Park ◽  
Chang Sik Pak ◽  
...  
Keyword(s):  
Stem Cells ◽  
Synergistic Effect ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Aged Mice

Promotion of Primary Murine Breast Cancer Growth and Metastasis by Adipose-Derived Stem Cells Is Reduced in the Presence of Autologous Fat Graft

2019 ◽  
Vol 143 (1) ◽  
pp. 137-147 ◽  
Author(s):  
Simon Gebremeskel ◽  
John Gencarelli ◽  
Alison J. Gareau ◽  
Terry Levatte ◽  
Anton Dugandzic ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Fat Graft ◽  
Cancer Growth ◽  
Adipose Derived Stem Cells ◽  
Autologous Fat ◽  
Autologous Fat Graft ◽  
Breast Cancer Growth

The Effect of Combined Use of Platelet Rich Plasma and Adipose Derived Stem Cells on Fat Graft Survival

2012 ◽  
Vol 130 ◽  
pp. 71
Author(s):  
Nevra Seyhan ◽  
Dogan Alhan ◽  
Ali Ugur Ural ◽  
Armagan Unal ◽  
Mustafa Cihad Avunduk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graft Survival ◽  
Platelet Rich Plasma ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Combined Use

Antiaging Treatment of the Facial Skin by Fat Graft and Adipose-Derived Stem Cells

2015 ◽  
Vol 135 (4) ◽  
pp. 999-1009 ◽  
Author(s):  
Luiz Charles-de-Sá ◽  
Natale Ferreira Gontijo-de-Amorim ◽  
Christina Maeda Takiya ◽  
Radovan Borojevic ◽  
Donatella Benati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Facial Skin

scholarly journals Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft

Cells ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 282 ◽  
Author(s):  
Pietro Gentile ◽  
Maria Piccinno ◽  
Claudio Calabrese
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Ex Vivo ◽  
Stromal Vascular Fraction ◽  
Enzymatic Digestion ◽  
Histological Evaluation ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Differentiation Potential ◽  
New Therapeutic Approaches

Human adipose-derived stem cells localize in the stromal-vascular portion, and can be ex vivo isolated using a combination of washing steps and enzymatic digestion. For this study, we undertook a histological evaluation of traditional fat graft compared with fat graft enriched with stromal vascular fraction cells isolated by the Celution™ system to assess the interactions between cells and adipose tissue before the breast injection. In addition, we reported on histological analyses of biopsies derived from fat grafted (traditional or enriched with SVFs) in the breast in order to assess the quality of the adipose tissue, fibrosis and vessels. The hASCs derived from enzymatic digestion were systematically characterized for growth features, phenotype and multi-potent differentiation potential. They fulfill the definition of mesenchymal stem cells, albeit with a higher neural phenotype profile. These cells also express genes that constitute the core circuitry of self-renewal such as OCT4, SOX2, NANOG and neurogenic lineage genes such as NEUROD1, PAX6 and SOX3. Such findings support the hypothesis that hASCs may have a potential usefulness in neurodegenerative conditions. These data can be helpful for the development of new therapeutic approaches in personalized medicine to assess safety and efficacy of the breast reconstruction.

Sign in / Sign up

Export Citation Format

Share Document